;;; GNU Guix --- Functional package management for GNU ;;; Copyright © 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021 Ricardo Wurmus ;;; Copyright © 2015, 2016, 2017, 2018 Ben Woodcroft ;;; Copyright © 2015, 2016, 2018, 2019, 2020 Pjotr Prins ;;; Copyright © 2015 Andreas Enge ;;; Copyright © 2016, 2020, 2021 Roel Janssen ;;; Copyright © 2016, 2017, 2018, 2019, 2020, 2021 Efraim Flashner ;;; Copyright © 2016, 2020 Marius Bakke ;;; Copyright © 2016, 2018 Raoul Bonnal ;;; Copyright © 2017, 2018 Tobias Geerinckx-Rice ;;; Copyright © 2017, 2021 Arun Isaac ;;; Copyright © 2018 Joshua Sierles, Nextjournal ;;; Copyright © 2018 Gábor Boskovits ;;; Copyright © 2018, 2019, 2020, 2021 Mădălin Ionel Patrașcu ;;; Copyright © 2019, 2020 Maxim Cournoyer ;;; Copyright © 2019 Brian Leung ;;; Copyright © 2019 Brett Gilio ;;; Copyright © 2020 Björn Höfling ;;; Copyright © 2020 Jakub Kądziołka ;;; Copyright © 2020 Pierre Langlois ;;; Copyright © 2020 Bonface Munyoki Kilyungi ;;; Copyright © 2021 Tim Howes ;;; Copyright © 2021 Hong Li ;;; ;;; This file is part of GNU Guix. ;;; ;;; GNU Guix is free software; you can redistribute it and/or modify it ;;; under the terms of the GNU General Public License as published by ;;; the Free Software Foundation; either version 3 of the License, or (at ;;; your option) any later version. ;;; ;;; GNU Guix is distributed in the hope that it will be useful, but ;;; WITHOUT ANY WARRANTY; without even the implied warranty of ;;; MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the ;;; GNU General Public License for more details. ;;; ;;; You should have received a copy of the GNU General Public License ;;; along with GNU Guix. If not, see . (define-module (gnu packages bioinformatics) #:use-module ((guix licenses) #:prefix license:) #:use-module (guix packages) #:use-module (guix utils) #:use-module (guix download) #:use-module (guix git-download) #:use-module (guix hg-download) #:use-module (guix build-system ant) #:use-module (guix build-system gnu) #:use-module (guix build-system cmake) #:use-module (guix build-system go) #:use-module (guix build-system haskell) #:use-module (guix build-system meson) #:use-module (guix build-system ocaml) #:use-module (guix build-system perl) #:use-module (guix build-system python) #:use-module (guix build-system qt) #:use-module (guix build-system r) #:use-module (guix build-system ruby) #:use-module (guix build-system scons) #:use-module (guix build-system trivial) #:use-module (guix deprecation) #:use-module (gnu packages) #:use-module (gnu packages admin) #:use-module (gnu packages assembly) #:use-module (gnu packages autotools) #:use-module (gnu packages algebra) #:use-module (gnu packages base) #:use-module (gnu packages bash) #:use-module (gnu packages bison) #:use-module (gnu packages bioconductor) #:use-module (gnu packages boost) #:use-module (gnu packages check) #:use-module (gnu packages code) #:use-module (gnu packages commencement) #:use-module (gnu packages cmake) #:use-module (gnu packages compression) #:use-module (gnu packages cpio) #:use-module (gnu packages cran) #:use-module (gnu packages curl) #:use-module (gnu packages documentation) #:use-module (gnu packages databases) #:use-module (gnu packages datastructures) #:use-module (gnu packages dlang) #:use-module (gnu packages file) #:use-module (gnu packages flex) #:use-module (gnu packages gawk) #:use-module (gnu packages gcc) #:use-module (gnu packages gd) #:use-module (gnu packages golang) #:use-module (gnu packages glib) #:use-module (gnu packages graph) #:use-module (gnu packages graphics) #:use-module (gnu packages graphviz) #:use-module (gnu packages groff) #:use-module (gnu packages gtk) #:use-module (gnu packages guile) #:use-module (gnu packages guile-xyz) #:use-module (gnu packages haskell-check) #:use-module (gnu packages haskell-web) #:use-module (gnu packages haskell-xyz) #:use-module (gnu packages image) #:use-module (gnu packages image-processing) #:use-module (gnu packages imagemagick) #:use-module (gnu packages java) #:use-module (gnu packages java-compression) #:use-module (gnu packages jemalloc) #:use-module (gnu packages linux) #:use-module (gnu packages lisp-xyz) #:use-module (gnu packages logging) #:use-module (gnu packages lsof) #:use-module (gnu packages machine-learning) #:use-module (gnu packages man) #:use-module (gnu packages maths) #:use-module (gnu packages mpi) #:use-module (gnu packages ncurses) #:use-module (gnu packages node) #:use-module (gnu packages ocaml) #:use-module (gnu packages pcre) #:use-module (gnu packages parallel) #:use-module (gnu packages pdf) #:use-module (gnu packages perl) #:use-module (gnu packages perl-check) #:use-module (gnu packages pkg-config) #:use-module (gnu packages popt) #:use-module (gnu packages protobuf) #:use-module (gnu packages python) #:use-module (gnu packages python-build) #:use-module (gnu packages python-check) #:use-module (gnu packages python-compression) #:use-module (gnu packages python-crypto) #:use-module (gnu packages python-science) #:use-module (gnu packages python-web) #:use-module (gnu packages python-xyz) #:use-module (gnu packages qt) #:use-module (gnu packages rdf) #:use-module (gnu packages readline) #:use-module (gnu packages rsync) #:use-module (gnu packages ruby) #:use-module (gnu packages serialization) #:use-module (gnu packages shells) #:use-module (gnu packages sphinx) #:use-module (gnu packages statistics) #:use-module (gnu packages swig) #:use-module (gnu packages tbb) #:use-module (gnu packages tex) #:use-module (gnu packages texinfo) #:use-module (gnu packages textutils) #:use-module (gnu packages time) #:use-module (gnu packages tls) #:use-module (gnu packages vim) #:use-module (gnu packages web) #:use-module (gnu packages wget) #:use-module (gnu packages xml) #:use-module (gnu packages xorg) #:use-module (srfi srfi-1) #:use-module (srfi srfi-26) #:use-module (ice-9 match)) (define-public aragorn (package (name "aragorn") (version "1.2.38") (source (origin (method url-fetch) (uri (string-append "http://mbio-serv2.mbioekol.lu.se/ARAGORN/Downloads/aragorn" version ".tgz")) (sha256 (base32 "09i1rg716smlbnixfm7q1ml2mfpaa2fpn3hwjg625ysmfwwy712b")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are no tests #:phases (modify-phases %standard-phases (delete 'configure) (replace 'build (lambda _ (invoke "gcc" "-O3" "-ffast-math" "-finline-functions" "-o" "aragorn" (string-append "aragorn" ,version ".c")) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (man (string-append out "/share/man/man1"))) (install-file "aragorn" bin) (install-file "aragorn.1" man)) #t))))) (home-page "http://mbio-serv2.mbioekol.lu.se/ARAGORN") (synopsis "Detect tRNA, mtRNA and tmRNA genes in nucleotide sequences") (description "Aragorn identifies transfer RNA, mitochondrial RNA and transfer-messenger RNA from nucleotide sequences, based on homology to known tRNA consensus sequences and RNA structure. It also outputs the secondary structure of the predicted RNA.") (license license:gpl2))) (define-public bamm (package (name "bamm") (version "1.7.3") (source (origin (method git-fetch) ;; BamM is not available on pypi. (uri (git-reference (url "https://github.com/Ecogenomics/BamM") (commit version) (recursive? #t))) (file-name (git-file-name name version)) (sha256 (base32 "1p83ahi984ipslxlg4yqy1gdnya9rkn1v71z8djgxkm9d2chw4c5")) (modules '((guix build utils))) (snippet `(begin ;; Delete bundled htslib. (delete-file-recursively "c/htslib-1.3.1") #t)))) (build-system python-build-system) (arguments `(#:python ,python-2 ; BamM is Python 2 only. ;; Do not use bundled libhts. Do use the bundled libcfu because it has ;; been modified from its original form. #:configure-flags (let ((htslib (assoc-ref %build-inputs "htslib"))) (list "--with-libhts-lib" (string-append htslib "/lib") "--with-libhts-inc" (string-append htslib "/include/htslib"))) #:phases (modify-phases %standard-phases (add-after 'unpack 'autogen (lambda _ (with-directory-excursion "c" (let ((sh (which "sh"))) (for-each make-file-writable (find-files "." ".*")) ;; Use autogen so that 'configure' works. (substitute* "autogen.sh" (("/bin/sh") sh)) (setenv "CONFIG_SHELL" sh) (invoke "./autogen.sh"))) #t)) (delete 'build) ;; Run tests after installation so compilation only happens once. (delete 'check) (add-after 'install 'wrap-executable (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (path (getenv "PATH"))) (wrap-program (string-append out "/bin/bamm") `("PATH" ":" prefix (,path)))) #t)) (add-after 'wrap-executable 'post-install-check (lambda* (#:key inputs outputs #:allow-other-keys) (setenv "PATH" (string-append (assoc-ref outputs "out") "/bin:" (getenv "PATH"))) (setenv "PYTHONPATH" (string-append (assoc-ref outputs "out") "/lib/python" (string-take (string-take-right (assoc-ref inputs "python") 5) 3) "/site-packages:" (getenv "PYTHONPATH"))) ;; There are 2 errors printed, but they are safe to ignore: ;; 1) [E::hts_open_format] fail to open file ... ;; 2) samtools view: failed to open ... (invoke "nosetests") #t))))) (native-inputs `(("autoconf" ,autoconf) ("automake" ,automake) ("libtool" ,libtool) ("zlib" ,zlib) ("python-nose" ,python2-nose) ("python-pysam" ,python2-pysam))) (inputs `(("htslib" ,htslib-1.3) ; At least one test fails on htslib-1.4+. ("samtools" ,samtools) ("bwa" ,bwa) ("grep" ,grep) ("sed" ,sed) ("coreutils" ,coreutils))) (propagated-inputs `(("python-numpy" ,python2-numpy))) (home-page "https://ecogenomics.github.io/BamM/") (synopsis "Metagenomics-focused BAM file manipulator") (description "BamM is a C library, wrapped in python, to efficiently generate and parse BAM files, specifically for the analysis of metagenomic data. For instance, it implements several methods to assess contig-wise read coverage.") (license license:lgpl3+))) (define-public bamtools (package (name "bamtools") (version "2.5.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/pezmaster31/bamtools") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0nfb2ypcx9959xnbz6wxh6py3xfizgmg8nrknxl95c507m9hmq8b")))) (build-system cmake-build-system) (arguments `(#:tests? #f ;no "check" target #:phases (modify-phases %standard-phases (add-before 'configure 'set-ldflags (lambda* (#:key outputs #:allow-other-keys) (setenv "LDFLAGS" (string-append "-Wl,-rpath=" (assoc-ref outputs "out") "/lib/bamtools")) #t))))) (inputs `(("zlib" ,zlib))) (home-page "https://github.com/pezmaster31/bamtools") (synopsis "C++ API and command-line toolkit for working with BAM data") (description "BamTools provides both a C++ API and a command-line toolkit for handling BAM files.") (license license:expat))) (define-public bamutils (package (name "bamutils") (version "1.0.13") (source (origin (method url-fetch) (uri (string-append "https://genome.sph.umich.edu/w/images/7/70/" "BamUtilLibStatGen." version ".tgz")) (sha256 (base32 "0asr1kmjbr3cyf4hkg865y8c2s30v87xvws4q6c8pyfi6wfd1h8n")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; There are no tests. #:make-flags `("USER_WARNINGS=-std=gnu++98" ,(string-append "INSTALLDIR=" (assoc-ref %outputs "out") "/bin")) #:phases (modify-phases %standard-phases (delete 'configure)))) (inputs `(("zlib" ,zlib))) (home-page "https://genome.sph.umich.edu/wiki/BamUtil") (synopsis "Programs for working on SAM/BAM files") (description "This package provides several programs that perform operations on SAM/BAM files. All of these programs are built into a single executable called @code{bam}.") (license license:gpl3+))) (define-public bcftools (package (name "bcftools") (version "1.12") (source (origin (method url-fetch) (uri (string-append "https://github.com/samtools/bcftools/" "releases/download/" version "/bcftools-" version ".tar.bz2")) (sha256 (base32 "1x94l1hy2pi3lbz0sxlbw0g6q5z5apcrhrlcwda94ns9n4r6a3ks")) (modules '((guix build utils))) (snippet '(begin ;; Delete bundled htslib. (delete-file-recursively "htslib-1.12") #t)))) (build-system gnu-build-system) (arguments `(#:configure-flags (list "--enable-libgsl") #:test-target "test" #:phases (modify-phases %standard-phases (add-before 'check 'patch-tests (lambda _ (substitute* "test/test.pl" (("/bin/bash") (which "bash"))) #t))))) (native-inputs `(("htslib" ,htslib) ("perl" ,perl))) (inputs `(("gsl" ,gsl) ("zlib" ,zlib))) (home-page "https://samtools.github.io/bcftools/") (synopsis "Utilities for variant calling and manipulating VCFs and BCFs") (description "BCFtools is a set of utilities that manipulate variant calls in the Variant Call Format (VCF) and its binary counterpart BCF. All commands work transparently with both VCFs and BCFs, both uncompressed and BGZF-compressed.") ;; The sources are dual MIT/GPL, but becomes GPL-only when USE_GPL=1. (license (list license:gpl3+ license:expat)))) (define-public bcftools-1.9 (package (inherit bcftools) (name "bcftools") (version "1.9") (source (origin (method url-fetch) (uri (string-append "https://github.com/samtools/bcftools/" "releases/download/" version "/bcftools-" version ".tar.bz2")) (sha256 (base32 "1j3h638i8kgihzyrlnpj82xg1b23sijibys9hvwari3fy7kd0dkg")) (modules '((guix build utils))) (snippet '(begin ;; Delete bundled htslib. (delete-file-recursively "htslib-1.9") #t)))) (build-system gnu-build-system) (native-inputs `(("htslib" ,htslib-1.9) ("perl" ,perl))))) (define-public bedops (package (name "bedops") (version "2.4.35") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/bedops/bedops") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0mmgsgwz5r9w76hzgxkxc9s9lkdhhaf7vr6i02b09vbswvs1fyqx")))) (build-system gnu-build-system) (arguments '(#:tests? #f #:make-flags (list (string-append "BINDIR=" %output "/bin")) #:phases (modify-phases %standard-phases (add-after 'unpack 'unpack-tarballs (lambda _ ;; FIXME: Bedops includes tarballs of minimally patched upstream ;; libraries jansson, zlib, and bzip2. We cannot just use stock ;; libraries because at least one of the libraries (zlib) is ;; patched to add a C++ function definition (deflateInit2cpp). ;; Until the Bedops developers offer a way to link against system ;; libraries we have to build the in-tree copies of these three ;; libraries. ;; See upstream discussion: ;; https://github.com/bedops/bedops/issues/124 ;; Unpack the tarballs to benefit from shebang patching. (with-directory-excursion "third-party" (invoke "tar" "xvf" "jansson-2.6.tar.bz2") (invoke "tar" "xvf" "zlib-1.2.7.tar.bz2") (invoke "tar" "xvf" "bzip2-1.0.6.tar.bz2")) ;; Disable unpacking of tarballs in Makefile. (substitute* "system.mk/Makefile.linux" (("^\tbzcat .*") "\t@echo \"not unpacking\"\n") (("\\./configure") "CONFIG_SHELL=bash ./configure")) (substitute* "third-party/zlib-1.2.7/Makefile.in" (("^SHELL=.*$") "SHELL=bash\n")) #t)) (delete 'configure)))) (home-page "https://github.com/bedops/bedops") (synopsis "Tools for high-performance genomic feature operations") (description "BEDOPS is a suite of tools to address common questions raised in genomic studies---mostly with regard to overlap and proximity relationships between data sets. It aims to be scalable and flexible, facilitating the efficient and accurate analysis and management of large-scale genomic data. BEDOPS provides tools that perform highly efficient and scalable Boolean and other set operations, statistical calculations, archiving, conversion and other management of genomic data of arbitrary scale. Tasks can be easily split by chromosome for distributing whole-genome analyses across a computational cluster.") (license license:gpl2+))) (define-public bedtools (package (name "bedtools") (version "2.30.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/arq5x/bedtools2/releases/" "download/v" version "/" "bedtools-" version ".tar.gz")) (sha256 (base32 "1f2hh79l7dn147c2xyfgf5wfjvlqfw32kjfnnh2n1qy6rpzx2fik")))) (build-system gnu-build-system) (arguments '(#:test-target "test" #:make-flags (list (string-append "prefix=" (assoc-ref %outputs "out"))) #:phases (modify-phases %standard-phases (delete 'configure)))) (native-inputs `(("python" ,python-wrapper))) (inputs `(("samtools" ,samtools) ("zlib" ,zlib))) (home-page "https://github.com/arq5x/bedtools2") (synopsis "Tools for genome analysis and arithmetic") (description "Collectively, the bedtools utilities are a swiss-army knife of tools for a wide-range of genomics analysis tasks. The most widely-used tools enable genome arithmetic: that is, set theory on the genome. For example, bedtools allows one to intersect, merge, count, complement, and shuffle genomic intervals from multiple files in widely-used genomic file formats such as BAM, BED, GFF/GTF, VCF.") (license license:expat))) ;; Later releases of bedtools produce files with more columns than ;; what Ribotaper expects. (define-public bedtools-2.18 (package (inherit bedtools) (name "bedtools") (version "2.18.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/arq5x/bedtools2/" "releases/download/v" version "/bedtools-" version ".tar.gz")) (sha256 (base32 "11rvca19ncg03kxd0wzlfx5ws7r3nisd0z8s9j9n182d8ksp2pxz")))) (arguments '(#:test-target "test" #:phases (modify-phases %standard-phases (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (for-each (lambda (file) (install-file file bin)) (find-files "bin" ".*"))) #t))))))) (define-public pbbam (package (name "pbbam") (version "0.23.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/PacificBiosciences/pbbam") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "0h9gkrpf2lrxklxp72xfl5bi3h5zcm5hprrya9gf0hr3xwlbpp0x")))) (build-system meson-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'find-googletest (lambda* (#:key inputs #:allow-other-keys) ;; It doesn't find gtest_main because there's no pkg-config file ;; for it. Find it another way. (substitute* "tests/meson.build" (("pbbam_gtest_dep = dependency\\('gtest_main'.*") (format #f "cpp = meson.get_compiler('cpp') pbbam_gtest_dep = cpp.find_library('gtest_main', dirs : '~a')\n" (assoc-ref inputs "googletest")))) #t))) ;; TODO: tests/pbbam_test cannot be linked ;; ld: tests/59830eb@@pbbam_test@exe/src_test_Accuracy.cpp.o: ;; undefined reference to symbol '_ZTIN7testing4TestE' ;; ld: /gnu/store/...-googletest-1.8.0/lib/libgtest.so: ;; error adding symbols: DSO missing from command line #:tests? #f #:configure-flags '("-Dtests=false"))) ;; These libraries are listed as "Required" in the pkg-config file. (propagated-inputs `(("htslib" ,htslib) ("zlib" ,zlib))) (inputs `(("boost" ,boost) ("samtools" ,samtools))) (native-inputs `(("googletest" ,googletest) ("pkg-config" ,pkg-config) ("python" ,python-wrapper))) ; for tests (home-page "https://github.com/PacificBiosciences/pbbam") (synopsis "Work with PacBio BAM files") (description "The pbbam software package provides components to create, query, and edit PacBio BAM files and associated indices. These components include a core C++ library, bindings for additional languages, and command-line utilities. This library is not intended to be used as a general-purpose BAM utility - all input and output BAMs must adhere to the PacBio BAM format specification. Non-PacBio BAMs will cause exceptions to be thrown.") (license license:bsd-3))) (define-public pbgzip (let ((commit "2b09f97b5f20b6d83c63a5c6b408d152e3982974")) (package (name "pbgzip") (version (git-version "0.0.0" "0" commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/nh13/pbgzip") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "1mlmq0v96irbz71bgw5zcc43g1x32zwnxx21a5p1f1ch4cikw1yd")))) (build-system gnu-build-system) (native-inputs `(("autoconf" ,autoconf) ("automake" ,automake))) (inputs `(("zlib" ,zlib))) (home-page "https://github.com/nh13/pbgzip") (synopsis "Parallel Block GZIP") (description "This package implements parallel block gzip. For many formats, in particular genomics data formats, data are compressed in fixed-length blocks such that they can be easily indexed based on a (genomic) coordinate order, since typically each block is sorted according to this order. This allows for each block to be individually compressed (deflated), or more importantly, decompressed (inflated), with the latter enabling random retrieval of data in large files (gigabytes to terabytes). @code{pbgzip} is not limited to any particular format, but certain features are tailored to genomics data formats when enabled. Parallel decompression is somewhat faster, but the true speedup comes during compression.") (license license:expat)))) (define-public blasr-libcpp (package (name "blasr-libcpp") (version "5.3.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/PacificBiosciences/blasr_libcpp") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "0cn5l42zyq67sj0g2imqkhayz2iqvv0a1pgpbmlq0qynjmsrbfd2")))) (build-system meson-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'link-with-hdf5 (lambda* (#:key inputs #:allow-other-keys) (let ((hdf5 (assoc-ref inputs "hdf5"))) (substitute* "meson.build" (("libblasr_deps = \\[" m) (string-append m (format #f "cpp.find_library('hdf5', dirs : '~a'), \ cpp.find_library('hdf5_cpp', dirs : '~a'), " hdf5 hdf5))))) #t)) (add-after 'unpack 'find-googletest (lambda* (#:key inputs #:allow-other-keys) ;; It doesn't find gtest_main because there's no pkg-config file ;; for it. Find it another way. (substitute* "unittest/meson.build" (("libblasr_gtest_dep = dependency\\('gtest_main'.*") (format #f "cpp = meson.get_compiler('cpp') libblasr_gtest_dep = cpp.find_library('gtest_main', dirs : '~a')\n" (assoc-ref inputs "googletest")))) #t))) ;; TODO: unittest/libblasr_unittest cannot be linked ;; ld: ;; unittest/df08227@@libblasr_unittest@exe/alignment_utils_FileUtils_gtest.cpp.o: ;; undefined reference to symbol ;; '_ZN7testing8internal9DeathTest6CreateEPKcPKNS0_2REES3_iPPS1_' ;; ld: /gnu/store/...-googletest-1.8.0/lib/libgtest.so: ;; error adding symbols: DSO missing from command line #:tests? #f #:configure-flags '("-Dtests=false"))) (inputs `(("boost" ,boost) ("hdf5" ,hdf5) ("pbbam" ,pbbam) ("zlib" ,zlib))) (native-inputs `(("googletest" ,googletest) ("pkg-config" ,pkg-config))) (home-page "https://github.com/PacificBiosciences/blasr_libcpp") (synopsis "Library for analyzing PacBio genomic sequences") (description "This package provides three libraries used by applications for analyzing PacBio genomic sequences. This library contains three sub-libraries: pbdata, hdf and alignment.") (license license:bsd-3))) (define-public blasr (package (name "blasr") (version "5.3.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/PacificBiosciences/blasr") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1skgy2mvz8gsgfh1gc2nfgwvpyzb1hpmp2cf2773h5wsj8nw22kl")))) (build-system meson-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'link-with-hdf5 (lambda* (#:key inputs #:allow-other-keys) (let ((hdf5 (assoc-ref inputs "hdf5"))) (substitute* "meson.build" (("blasr_deps = \\[" m) (string-append m (format #f "cpp.find_library('hdf5', dirs : '~a'), \ cpp.find_library('hdf5_cpp', dirs : '~a'), " hdf5 hdf5))))) #t))) ;; Tests require "cram" executable, which is not packaged. #:tests? #f #:configure-flags '("-Dtests=false"))) (inputs `(("boost" ,boost) ("blasr-libcpp" ,blasr-libcpp) ("hdf5" ,hdf5) ("pbbam" ,pbbam) ("zlib" ,zlib))) (native-inputs `(("pkg-config" ,pkg-config))) (home-page "https://github.com/PacificBiosciences/blasr") (synopsis "PacBio long read aligner") (description "Blasr is a genomic sequence aligner for processing PacBio long reads.") (license license:bsd-3))) (define-public ribotaper (package (name "ribotaper") (version "1.3.1") (source (origin (method url-fetch) (uri (string-append "https://ohlerlab.mdc-berlin.de/" "files/RiboTaper/RiboTaper_Version_" version ".tar.gz")) (sha256 (base32 "0ykjbps1y3z3085q94npw8i9x5gldc6shy8vlc08v76zljsm07hv")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'install 'wrap-executables (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out"))) (for-each (lambda (script) (wrap-program (string-append out "/bin/" script) `("R_LIBS_SITE" ":" = (,(getenv "R_LIBS_SITE"))))) '("create_annotations_files.bash" "create_metaplots.bash" "Ribotaper_ORF_find.sh" "Ribotaper.sh"))) #t))))) (inputs `(("bedtools" ,bedtools-2.18) ("samtools" ,samtools-0.1) ("r-minimal" ,r-minimal) ("r-foreach" ,r-foreach) ("r-xnomial" ,r-xnomial) ("r-domc" ,r-domc) ("r-multitaper" ,r-multitaper) ("r-seqinr" ,r-seqinr))) (home-page "https://ohlerlab.mdc-berlin.de/software/RiboTaper_126/") (synopsis "Define translated ORFs using ribosome profiling data") (description "Ribotaper is a method for defining translated @dfn{open reading frames} (ORFs) using ribosome profiling (ribo-seq) data. This package provides the Ribotaper pipeline.") (license license:gpl3+))) (define-public ribodiff (package (name "ribodiff") (version "0.2.2") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ratschlab/RiboDiff") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0x75nlp7qnmm64jasbi6l21f2cy99r2cjyl6b4hr8zf2bq22drnz")))) (build-system python-build-system) (arguments `(#:python ,python-2 #:phases (modify-phases %standard-phases ;; Generate an installable executable script wrapper. (add-after 'unpack 'patch-setup.py (lambda _ (substitute* "setup.py" (("^(.*)packages=.*" line prefix) (string-append line "\n" prefix "scripts=['scripts/TE.py'],\n"))) #t))))) (inputs `(("python-numpy" ,python2-numpy) ("python-matplotlib" ,python2-matplotlib) ("python-scipy" ,python2-scipy) ("python-statsmodels" ,python2-statsmodels))) (native-inputs `(("python-mock" ,python2-mock) ("python-nose" ,python2-nose))) (home-page "https://public.bmi.inf.ethz.ch/user/zhongy/RiboDiff/") (synopsis "Detect translation efficiency changes from ribosome footprints") (description "RiboDiff is a statistical tool that detects the protein translational efficiency change from Ribo-Seq (ribosome footprinting) and RNA-Seq data. It uses a generalized linear model to detect genes showing difference in translational profile taking mRNA abundance into account. It facilitates us to decipher the translational regulation that behave independently with transcriptional regulation.") (license license:gpl3+))) (define-public bioawk (package (name "bioawk") (version "1.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/lh3/bioawk") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1pxc3zdnirxbf9a0az698hd8xdik7qkhypm7v6hn922x8y9qmspm")))) (build-system gnu-build-system) (inputs `(("zlib" ,zlib))) (native-inputs `(("bison" ,bison))) (arguments `(#:tests? #f ; There are no tests to run. ;; Bison must generate files, before other targets can build. #:parallel-build? #f #:phases (modify-phases %standard-phases (delete 'configure) ; There is no configure phase. (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (man (string-append out "/share/man/man1"))) (mkdir-p man) (copy-file "awk.1" (string-append man "/bioawk.1")) (install-file "bioawk" bin)) #t))))) (home-page "https://github.com/lh3/bioawk") (synopsis "AWK with bioinformatics extensions") (description "Bioawk is an extension to Brian Kernighan's awk, adding the support of several common biological data formats, including optionally gzip'ed BED, GFF, SAM, VCF, FASTA/Q and TAB-delimited formats with column names. It also adds a few built-in functions and a command line option to use TAB as the input/output delimiter. When the new functionality is not used, bioawk is intended to behave exactly the same as the original BWK awk.") (license license:x11))) (define-public python-htsget (package (name "python-htsget") (version "0.2.5") (source (origin (method url-fetch) (uri (pypi-uri "htsget" version)) (sha256 (base32 "0ic07q85vhw9djf23k57b21my7i5xp400m8gfqgr5gcryqvdr0yk")))) (build-system python-build-system) (native-inputs `(("python-setuptools-scm" ,python-setuptools-scm))) (propagated-inputs `(("python-humanize" ,python-humanize) ("python-requests" ,python-requests) ("python-six" ,python-six))) (home-page "https://pypi.org/project/htsget/") (synopsis "Python API and command line interface for the GA4GH htsget API") (description "This package is a client implementation of the GA4GH htsget protocol. It provides a simple and reliable way to retrieve genomic data from servers supporting the protocol.") (license license:asl2.0))) (define-public python-pybedtools (package (name "python-pybedtools") (version "0.8.2") (source (origin (method url-fetch) (uri (pypi-uri "pybedtools" version)) (sha256 (base32 "0wc7z8g8prgdx7n5chjva2fdq03wiwhqisjjxzkjg1j5k5ha7151")))) (build-system python-build-system) (arguments `(#:modules ((ice-9 ftw) (srfi srfi-1) (srfi srfi-26) (guix build utils) (guix build python-build-system)) ;; See https://github.com/daler/pybedtools/issues/192 #:phases (modify-phases %standard-phases (add-after 'unpack 'disable-broken-tests (lambda _ (substitute* "pybedtools/test/test_helpers.py" ;; Requires internet access. (("def test_chromsizes") "def _do_not_test_chromsizes") ;; Broken as a result of the workaround used in the check phase ;; (see: https://github.com/daler/pybedtools/issues/192). (("def test_getting_example_beds") "def _do_not_test_getting_example_beds")) ;; This issue still occurs on python2 (substitute* "pybedtools/test/test_issues.py" (("def test_issue_303") "def _test_issue_303")))) ;; TODO: Remove phase after it's part of PYTHON-BUILD-SYSTEM. ;; build system. ;; Force the Cythonization of C++ files to guard against compilation ;; problems. (add-after 'unpack 'remove-cython-generated-files (lambda _ (let ((cython-sources (map (cut string-drop-right <> 4) (find-files "." "\\.pyx$"))) (c/c++-files (find-files "." "\\.(c|cpp|cxx)$"))) (define (strip-extension filename) (string-take filename (string-index-right filename #\.))) (define (cythonized? c/c++-file) (member (strip-extension c/c++-file) cython-sources)) (for-each delete-file (filter cythonized? c/c++-files)) #t))) (add-after 'remove-cython-generated-files 'generate-cython-extensions (lambda _ (invoke "python" "setup.py" "cythonize"))) (replace 'check (lambda _ (let* ((build-root-directory (string-append (getcwd) "/build/")) (build (string-append build-root-directory (find (cut string-prefix? "lib" <>) (scandir build-root-directory))))) (setenv "PYTHONPATH" (string-append build ":" (getenv "PYTHONPATH")))) ;; The tests need to be run from elsewhere... (mkdir-p "/tmp/test") (copy-recursively "pybedtools/test" "/tmp/test") (with-directory-excursion "/tmp/test" (invoke "pytest" "-v" "--doctest-modules"))))))) (propagated-inputs `(("bedtools" ,bedtools) ("samtools" ,samtools) ("python-matplotlib" ,python-matplotlib) ("python-pysam" ,python-pysam) ("python-pyyaml" ,python-pyyaml))) (native-inputs `(("python-numpy" ,python-numpy) ("python-pandas" ,python-pandas) ("python-cython" ,python-cython) ("kentutils" ,kentutils) ; for bedGraphToBigWig ("python-six" ,python-six) ;; For the test suite. ("python-pytest" ,python-pytest) ("python-psutil" ,python-psutil))) (home-page "https://pythonhosted.org/pybedtools/") (synopsis "Python wrapper for BEDtools programs") (description "pybedtools is a Python wrapper for Aaron Quinlan's BEDtools programs, which are widely used for genomic interval manipulation or \"genome algebra\". pybedtools extends BEDTools by offering feature-level manipulations from with Python.") (license license:gpl2+))) (define-public python2-pybedtools (let ((pybedtools (package-with-python2 python-pybedtools))) (package (inherit pybedtools) (native-inputs `(("python2-pathlib" ,python2-pathlib) ,@(package-native-inputs pybedtools)))))) (define-public python-biom-format (package (name "python-biom-format") (version "2.1.7") (source (origin (method git-fetch) ;; Use GitHub as source because PyPI distribution does not contain ;; test data: https://github.com/biocore/biom-format/issues/693 (uri (git-reference (url "https://github.com/biocore/biom-format") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1rna16lyk5aqhnv0dp77wwaplias93f1vw28ad3jmyw6hwkai05v")) (modules '((guix build utils))) (snippet '(begin ;; Delete generated C files. (for-each delete-file (find-files "." "\\.c")) #t)))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'use-cython (lambda _ (setenv "USE_CYTHON" "1") #t)) (add-after 'unpack 'disable-broken-tests (lambda _ (substitute* "biom/tests/test_cli/test_validate_table.py" (("^(.+)def test_invalid_hdf5" m indent) (string-append indent "@npt.dec.skipif(True, msg='Guix')\n" m))) (substitute* "biom/tests/test_table.py" (("^(.+)def test_from_hdf5_issue_731" m indent) (string-append indent "@npt.dec.skipif(True, msg='Guix')\n" m))) #t)) (add-before 'reset-gzip-timestamps 'make-files-writable (lambda* (#:key outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) (for-each (lambda (file) (chmod file #o644)) (find-files out "\\.gz")) #t)))))) (propagated-inputs `(("python-numpy" ,python-numpy) ("python-scipy" ,python-scipy) ("python-flake8" ,python-flake8) ("python-future" ,python-future) ("python-click" ,python-click) ("python-h5py" ,python-h5py) ;; FIXME: Upgrade to pandas 1.0 when ;; https://github.com/biocore/biom-format/issues/837 is resolved. ("python-pandas" ,python-pandas-0.25))) (native-inputs `(("python-cython" ,python-cython) ("python-pytest" ,python-pytest) ("python-pytest-cov" ,python-pytest-cov) ("python-nose" ,python-nose))) (home-page "http://www.biom-format.org") (synopsis "Biological Observation Matrix (BIOM) format utilities") (description "The BIOM file format is designed to be a general-use format for representing counts of observations e.g. operational taxonomic units, KEGG orthology groups or lipid types, in one or more biological samples e.g. microbiome samples, genomes, metagenomes.") (license license:bsd-3) (properties `((python2-variant . ,(delay python2-biom-format)))))) (define-public python2-biom-format (let ((base (package-with-python2 (strip-python2-variant python-biom-format)))) (package (inherit base) (arguments (substitute-keyword-arguments (package-arguments base) ((#:phases phases) `(modify-phases ,phases ;; Do not require the unmaintained pyqi library. (add-after 'unpack 'remove-pyqi (lambda _ (substitute* "setup.py" (("install_requires.append\\(\"pyqi\"\\)") "pass")) #t))))))))) (define-public python-pairtools (package (name "python-pairtools") (version "0.3.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/mirnylab/pairtools") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0gr8y13q7sd6yai6df4aavl2470n1f9s3cib6r473z4hr8hcbwmc")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'fix-references (lambda _ (substitute* '("pairtools/pairtools_merge.py" "pairtools/pairtools_sort.py") (("/bin/bash") (which "bash"))) #t)) (replace 'check (lambda* (#:key inputs outputs #:allow-other-keys) (add-installed-pythonpath inputs outputs) (with-directory-excursion "/tmp" (invoke "pytest" "-v"))))))) (native-inputs `(("python-cython" ,python-cython) ("python-nose" ,python-nose) ("python-pytest" ,python-pytest))) (inputs `(("python" ,python-wrapper))) (propagated-inputs `(("htslib" ,htslib) ; for bgzip, looked up in PATH ("samtools" ,samtools) ; looked up in PATH ("lz4" ,lz4) ; for lz4c ("python-click" ,python-click) ("python-numpy" ,python-numpy))) (home-page "https://github.com/mirnylab/pairtools") (synopsis "Process mapped Hi-C data") (description "Pairtools is a simple and fast command-line framework to process sequencing data from a Hi-C experiment. Process pair-end sequence alignments and perform the following operations: @itemize @item detect ligation junctions (a.k.a. Hi-C pairs) in aligned paired-end sequences of Hi-C DNA molecules @item sort @code{.pairs} files for downstream analyses @item detect, tag and remove PCR/optical duplicates @item generate extensive statistics of Hi-C datasets @item select Hi-C pairs given flexibly defined criteria @item restore @code{.sam} alignments from Hi-C pairs. @end itemize ") (license license:expat))) (define-public bioperl-minimal (let* ((inputs `(("perl-module-build" ,perl-module-build) ("perl-data-stag" ,perl-data-stag) ("perl-libwww" ,perl-libwww) ("perl-uri" ,perl-uri))) (transitive-inputs (map (compose package-name cadr) (delete-duplicates (concatenate (map (compose package-transitive-target-inputs cadr) inputs)))))) (package (name "bioperl-minimal") (version "1.7.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/bioperl/bioperl-live") (commit (string-append "release-" (string-map (lambda (c) (if (char=? c #\.) #\- c)) version))))) (file-name (git-file-name name version)) (sha256 (base32 "0wl8yvzcls59pwwk6m8ahy87pwg6nnibzy5cldbvmcwg2x2w7783")))) (build-system perl-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'install 'wrap-programs (lambda* (#:key outputs #:allow-other-keys) ;; Make sure all executables in "bin" find the required Perl ;; modules at runtime. As the PERL5LIB variable contains also ;; the paths of native inputs, we pick the transitive target ;; inputs from %build-inputs. (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin/")) (path (string-join (cons (string-append out "/lib/perl5/site_perl") (map (lambda (name) (assoc-ref %build-inputs name)) ',transitive-inputs)) ":"))) (for-each (lambda (file) (wrap-program file `("PERL5LIB" ":" prefix (,path)))) (find-files bin "\\.pl$")) #t)))))) (inputs inputs) (native-inputs `(("perl-test-most" ,perl-test-most))) (home-page "https://metacpan.org/release/BioPerl") (synopsis "Bioinformatics toolkit") (description "BioPerl is the product of a community effort to produce Perl code which is useful in biology. Examples include Sequence objects, Alignment objects and database searching objects. These objects not only do what they are advertised to do in the documentation, but they also interact - Alignment objects are made from the Sequence objects, Sequence objects have access to Annotation and SeqFeature objects and databases, Blast objects can be converted to Alignment objects, and so on. This means that the objects provide a coordinated and extensible framework to do computational biology.") (license license:perl-license)))) (define-public perl-bio-db-hts (package (name "perl-bio-db-hts") (version "3.01") (source (origin (method url-fetch) (uri (string-append "mirror://cpan/authors/id/A/AV/AVULLO/Bio-DB-HTS-" version ".tar.gz")) (sha256 (base32 "0hjg0igfkpvh27zdkdr6pa7cqm9n6r7cwz0np74cl4wmawgvr9hj")))) (build-system perl-build-system) (native-inputs `(("perl-module-build" ,perl-module-build) ("pkg-config" ,pkg-config))) (propagated-inputs `(("bioperl-minimal" ,bioperl-minimal) ("htslib" ,htslib-1.9))) (home-page "https://metacpan.org/release/Bio-DB-HTS") (synopsis "Perl interface to HTS library for DNA sequencing") (description "This is a Perl interface to the HTS library for DNA sequencing.") (license license:asl2.0))) (define-public python-biopython (package (name "python-biopython") (version "1.70") (source (origin (method url-fetch) ;; use PyPi rather than biopython.org to ease updating (uri (pypi-uri "biopython" version)) (sha256 (base32 "0nz4n9d2y2dg849gn1z0vjlkwcpzzkzy3fij7x94a6ixy2c54z2a")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-before 'check 'set-home ;; Some tests require a home directory to be set. (lambda _ (setenv "HOME" "/tmp") #t))))) (propagated-inputs `(("python-numpy" ,python-numpy))) (home-page "https://biopython.org/") (synopsis "Tools for biological computation in Python") (description "Biopython is a set of tools for biological computation including parsers for bioinformatics files into Python data structures; interfaces to common bioinformatics programs; a standard sequence class and tools for performing common operations on them; code to perform data classification; code for dealing with alignments; code making it easy to split up parallelizable tasks into separate processes; and more.") (license (license:non-copyleft "http://www.biopython.org/DIST/LICENSE")))) (define-public python2-biopython (package-with-python2 python-biopython)) (define-public python-fastalite (package (name "python-fastalite") (version "0.3") (source (origin (method url-fetch) (uri (pypi-uri "fastalite" version)) (sha256 (base32 "1qli6pxp77i9xn2wfciq2zaxhl82bdxb33cpzqzj1z25yd036wqj")))) (build-system python-build-system) (arguments `(#:tests? #f)) ; Test data is not distributed. (home-page "https://github.com/nhoffman/fastalite") (synopsis "Simplest possible FASTA parser") (description "This library implements a FASTA and a FASTQ parser without relying on a complex dependency tree.") (license license:expat))) (define-public python2-fastalite (package-with-python2 python-fastalite)) (define-public bpp-core ;; The last release was in 2014 and the recommended way to install from source ;; is to clone the git repository, so we do this. ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page (let ((commit "7d8bced0d1a87291ea8dd7046b7fb5ff9c35c582")) (package (name "bpp-core") (version (string-append "2.2.0-1." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "http://biopp.univ-montp2.fr/git/bpp-core") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "10djsq5vlnkilv436gnmh4irpk49v29pa69r6xiryg32xmvn909j")))) (build-system cmake-build-system) (arguments `(#:parallel-build? #f)) (home-page "http://biopp.univ-montp2.fr") (synopsis "C++ libraries for Bioinformatics") (description "Bio++ is a set of C++ libraries for Bioinformatics, including sequence analysis, phylogenetics, molecular evolution and population genetics. It is Object Oriented and is designed to be both easy to use and computer efficient. Bio++ intends to help programmers to write computer expensive programs, by providing them a set of re-usable tools.") (license license:cecill-c)))) (define-public bpp-phyl ;; The last release was in 2014 and the recommended way to install from source ;; is to clone the git repository, so we do this. ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page (let ((commit "0c07167b629f68b569bf274d1ad0c4af83276ae2")) (package (name "bpp-phyl") (version (string-append "2.2.0-1." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "http://biopp.univ-montp2.fr/git/bpp-phyl") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "1ssjgchzwj3iai26kyly7gwkdv8sk59nqhkb1wpap3sf5m6kyllh")))) (build-system cmake-build-system) (arguments `(#:parallel-build? #f ;; If out-of-source, test data is not copied into the build directory ;; so the tests fail. #:out-of-source? #f)) (inputs `(("bpp-core" ,bpp-core) ("bpp-seq" ,bpp-seq))) (home-page "http://biopp.univ-montp2.fr") (synopsis "Bio++ phylogenetic Library") (description "Bio++ is a set of C++ libraries for Bioinformatics, including sequence analysis, phylogenetics, molecular evolution and population genetics. This library provides phylogenetics-related modules.") (license license:cecill-c)))) (define-public bpp-popgen ;; The last release was in 2014 and the recommended way to install from source ;; is to clone the git repository, so we do this. ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page (let ((commit "e472bac9b1a148803895d747cd6d0c5904f85d9f")) (package (name "bpp-popgen") (version (string-append "2.2.0-1." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "http://biopp.univ-montp2.fr/git/bpp-popgen") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "0yn82dzn1n5629nzja68xfrhi655709rjanyryb36vzkmymy6dw5")))) (build-system cmake-build-system) (arguments `(#:parallel-build? #f #:tests? #f)) ; There are no tests. (inputs `(("bpp-core" ,bpp-core) ("bpp-seq" ,bpp-seq))) (home-page "http://biopp.univ-montp2.fr") (synopsis "Bio++ population genetics library") (description "Bio++ is a set of C++ libraries for Bioinformatics, including sequence analysis, phylogenetics, molecular evolution and population genetics. This library provides population genetics-related modules.") (license license:cecill-c)))) (define-public bpp-seq ;; The last release was in 2014 and the recommended way to install from source ;; is to clone the git repository, so we do this. ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page (let ((commit "6cfa07965ce152e5598a89df2fa80a75973bfa33")) (package (name "bpp-seq") (version (string-append "2.2.0-1." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "http://biopp.univ-montp2.fr/git/bpp-seq") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "1nys5jq7jqvdg40d91wsmj3q2yzy4276cp7sp44n67p468f27zf2")))) (build-system cmake-build-system) (arguments `(#:parallel-build? #f ;; If out-of-source, test data is not copied into the build directory ;; so the tests fail. #:out-of-source? #f)) (inputs `(("bpp-core" ,bpp-core))) (home-page "http://biopp.univ-montp2.fr") (synopsis "Bio++ sequence library") (description "Bio++ is a set of C++ libraries for Bioinformatics, including sequence analysis, phylogenetics, molecular evolution and population genetics. This library provides sequence-related modules.") (license license:cecill-c)))) (define-public bppsuite ;; The last release was in 2014 and the recommended way to install from source ;; is to clone the git repository, so we do this. ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page (let ((commit "c516147f57aa50961121cd505bed52cd7603698b")) (package (name "bppsuite") (version (string-append "2.2.0-1." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "http://biopp.univ-montp2.fr/git/bppsuite") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "1y87pxvw0jxjizhq2dr9g2r91md45k1p9ih2sl1yy1y3p934l2kb")))) (build-system cmake-build-system) (arguments `(#:parallel-build? #f #:tests? #f)) ; There are no tests. (native-inputs `(("groff" ,groff) ("man-db" ,man-db) ("texinfo" ,texinfo))) (inputs `(("bpp-core" ,bpp-core) ("bpp-seq" ,bpp-seq) ("bpp-phyl" ,bpp-phyl) ("bpp-phyl" ,bpp-popgen))) (home-page "http://biopp.univ-montp2.fr") (synopsis "Bioinformatics tools written with the Bio++ libraries") (description "Bio++ is a set of C++ libraries for Bioinformatics, including sequence analysis, phylogenetics, molecular evolution and population genetics. This package provides command line tools using the Bio++ library.") (license license:cecill-c)))) (define-public blast+ (package (name "blast+") (version "2.11.0") (source (origin (method url-fetch) (uri (string-append "https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/" version "/ncbi-blast-" version "+-src.tar.gz")) (sha256 (base32 "0m0r9vkw631ky1za1wilsfk9k9spwqh22nkrb9a57rbwmrc1i3nq")) (modules '((guix build utils))) (snippet '(begin ;; Remove bundled bzip2, zlib and pcre. (delete-file-recursively "c++/src/util/compress/bzip2") (delete-file-recursively "c++/src/util/compress/zlib") (delete-file-recursively "c++/src/util/regexp") (substitute* "c++/src/util/compress/Makefile.in" (("bzip2 zlib api") "api")) ;; Remove useless msbuild directory (delete-file-recursively "c++/src/build-system/project_tree_builder/msbuild") #t)))) (build-system gnu-build-system) (arguments `(;; There are two(!) tests for this massive library, and both fail with ;; "unparsable timing stats". ;; ERR [127] -- [serial/datatool] datatool.sh (unparsable timing stats) ;; ERR [127] -- [serial/datatool] datatool_xml.sh (unparsable timing stats) #:tests? #f #:out-of-source? #t #:parallel-build? #f ; not supported #:phases (modify-phases %standard-phases (add-before 'configure 'set-HOME ;; $HOME needs to be set at some point during the configure phase (lambda _ (setenv "HOME" "/tmp") #t)) (add-after 'unpack 'enter-dir (lambda _ (chdir "c++") #t)) (add-after 'enter-dir 'fix-build-system (lambda _ (define (which* cmd) (cond ((string=? cmd "date") ;; make call to "date" deterministic "date -d @0") ((which cmd) => identity) (else (format (current-error-port) "WARNING: Unable to find absolute path for ~s~%" cmd) #f))) ;; Rewrite hardcoded paths to various tools (substitute* (append '("src/build-system/configure.ac" "src/build-system/configure" "src/build-system/helpers/run_with_lock.c" "scripts/common/impl/if_diff.sh" "scripts/common/impl/run_with_lock.sh" "src/build-system/Makefile.configurables.real" "src/build-system/Makefile.in.top" "src/build-system/Makefile.meta.gmake=no" "src/build-system/Makefile.meta.in" "src/build-system/Makefile.meta_l" "src/build-system/Makefile.meta_p" "src/build-system/Makefile.meta_r" "src/build-system/Makefile.mk.in" "src/build-system/Makefile.requirements" "src/build-system/Makefile.rules_with_autodep.in") (find-files "scripts/common/check" "\\.sh$")) (("(/usr/bin/|/bin/)([a-z][-_.a-z]*)" all dir cmd) (or (which* cmd) all))) (substitute* (find-files "src/build-system" "^config.*") (("LN_S=/bin/\\$LN_S") (string-append "LN_S=" (which "ln"))) (("^PATH=.*") "")) ;; rewrite "/var/tmp" in check script (substitute* "scripts/common/check/check_make_unix.sh" (("/var/tmp") "/tmp")) ;; do not reset PATH (substitute* (find-files "scripts/common/impl/" "\\.sh$") (("^ *PATH=.*") "") (("action=/bin/") "action=") (("export PATH") ":")) #t)) (replace 'configure (lambda* (#:key inputs outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out")) (lib (string-append (assoc-ref outputs "lib") "/lib")) (include (string-append (assoc-ref outputs "include") "/include/ncbi-tools++"))) ;; The 'configure' script doesn't recognize things like ;; '--enable-fast-install'. (invoke "./configure.orig" (string-append "--with-build-root=" (getcwd) "/build") (string-append "--prefix=" out) (string-append "--libdir=" lib) (string-append "--includedir=" include) (string-append "--with-bz2=" (assoc-ref inputs "bzip2")) (string-append "--with-z=" (assoc-ref inputs "zlib")) (string-append "--with-pcre=" (assoc-ref inputs "pcre")) ;; Each library is built twice by default, once ;; with "-static" in its name, and again ;; without. "--without-static" "--with-dll") #t)))))) (outputs '("out" ; 21 MB "lib" ; 226 MB "include")) ; 33 MB (inputs `(("bzip2" ,bzip2) ("lmdb" ,lmdb) ("zlib" ,zlib) ("pcre" ,pcre) ("perl" ,perl) ("python" ,python-wrapper))) (native-inputs `(("cpio" ,cpio))) (home-page "https://blast.ncbi.nlm.nih.gov") (synopsis "Basic local alignment search tool") (description "BLAST is a popular method of performing a DNA or protein sequence similarity search, using heuristics to produce results quickly. It also calculates an “expect value” that estimates how many matches would have occurred at a given score by chance, which can aid a user in judging how much confidence to have in an alignment.") ;; Most of the sources are in the public domain, with the following ;; exceptions: ;; * Expat: ;; * ./c++/include/util/bitset/ ;; * ./c++/src/html/ncbi_menu*.js ;; * Boost license: ;; * ./c++/include/util/impl/floating_point_comparison.hpp ;; * LGPL 2+: ;; * ./c++/include/dbapi/driver/odbc/unix_odbc/ ;; * ASL 2.0: ;; * ./c++/src/corelib/teamcity_* (license (list license:public-domain license:expat license:boost1.0 license:lgpl2.0+ license:asl2.0)))) (define-public bless (package (name "bless") (version "1p02") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/bless-ec/bless.v" version ".tgz")) (sha256 (base32 "0rm0gw2s18dqwzzpl3c2x1z05ni2v0xz5dmfk3d33j6g4cgrlrdd")) (modules '((guix build utils))) (snippet `(begin ;; Remove bundled boost, pigz, zlib, and .git directory ;; FIXME: also remove bundled sources for murmurhash3 and ;; kmc once packaged. (delete-file-recursively "boost") (delete-file-recursively "pigz") (delete-file-recursively "google-sparsehash") (delete-file-recursively "zlib") (delete-file-recursively ".git") #t)))) (build-system gnu-build-system) (arguments '(#:tests? #f ;no "check" target #:make-flags (list (string-append "ZLIB=" (assoc-ref %build-inputs "zlib:static") "/lib/libz.a") (string-append "LDFLAGS=" (string-join '("-lboost_filesystem" "-lboost_system" "-lboost_iostreams" "-lz" "-fopenmp")))) #:phases (modify-phases %standard-phases (add-after 'unpack 'do-not-build-bundled-pigz (lambda* (#:key inputs outputs #:allow-other-keys) (substitute* "Makefile" (("cd pigz/pigz-2.3.3; make") "")) #t)) (add-after 'unpack 'patch-paths-to-executables (lambda* (#:key inputs outputs #:allow-other-keys) (substitute* "parse_args.cpp" (("kmc_binary = .*") (string-append "kmc_binary = \"" (assoc-ref outputs "out") "/bin/kmc\";")) (("pigz_binary = .*") (string-append "pigz_binary = \"" (assoc-ref inputs "pigz") "/bin/pigz\";"))) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (for-each (lambda (file) (install-file file bin)) '("bless" "kmc/bin/kmc")) #t))) (delete 'configure)))) (native-inputs `(("perl" ,perl))) (inputs `(("openmpi" ,openmpi) ("boost" ,boost) ("sparsehash" ,sparsehash) ("pigz" ,pigz) ("zlib:static" ,zlib "static") ("zlib" ,zlib))) (supported-systems '("x86_64-linux")) (home-page "https://sourceforge.net/p/bless-ec/wiki/Home/") (synopsis "Bloom-filter-based error correction tool for NGS reads") (description "@dfn{Bloom-filter-based error correction solution for high-throughput sequencing reads} (BLESS) uses a single minimum-sized bloom filter is a correction tool for genomic reads produced by @dfn{Next-generation sequencing} (NGS). BLESS produces accurate correction results with much less memory compared with previous solutions and is also able to tolerate a higher false-positive rate. BLESS can extend reads like DNA assemblers to correct errors at the end of reads.") (license license:gpl3+))) (define-public bowtie (package (name "bowtie") (version "2.3.4.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/BenLangmead/bowtie2") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1zl3cf327y2p7p03cavymbh7b00djc7lncfaqih33n96iy9q8ibp")) (modules '((guix build utils))) (snippet '(begin (substitute* "Makefile" ;; replace BUILD_HOST and BUILD_TIME for deterministic build (("-DBUILD_HOST=.*") "-DBUILD_HOST=\"\\\"guix\\\"\"") (("-DBUILD_TIME=.*") "-DBUILD_TIME=\"\\\"0\\\"\"")) #t)))) (build-system gnu-build-system) (arguments '(#:make-flags (list "allall" "WITH_TBB=1" (string-append "prefix=" (assoc-ref %outputs "out"))) #:phases (modify-phases %standard-phases (delete 'configure) (replace 'check (lambda _ (invoke "perl" "scripts/test/simple_tests.pl" "--bowtie2=./bowtie2" "--bowtie2-build=./bowtie2-build") #t))))) (inputs `(("tbb" ,tbb) ("zlib" ,zlib) ("python" ,python-wrapper))) (native-inputs `(("perl" ,perl) ("perl-clone" ,perl-clone) ("perl-test-deep" ,perl-test-deep) ("perl-test-simple" ,perl-test-simple))) (home-page "http://bowtie-bio.sourceforge.net/bowtie2/index.shtml") (synopsis "Fast and sensitive nucleotide sequence read aligner") (description "Bowtie 2 is a fast and memory-efficient tool for aligning sequencing reads to long reference sequences. It is particularly good at aligning reads of about 50 up to 100s or 1,000s of characters, and particularly good at aligning to relatively long (e.g. mammalian) genomes. Bowtie 2 indexes the genome with an FM Index to keep its memory footprint small: for the human genome, its memory footprint is typically around 3.2 GB. Bowtie 2 supports gapped, local, and paired-end alignment modes.") (supported-systems '("x86_64-linux")) (license license:gpl3+))) (define-public bowtie1 (package (name "bowtie1") (version "1.3.0") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/bowtie-bio/bowtie/" version "/bowtie-" version "-src.zip")) (sha256 (base32 "11dbihdnrizc6qhx9xsw77w3q5ssx642alaqzvhxx32ak9glvq04")) (modules '((guix build utils))) (snippet '(substitute* "Makefile" ;; replace BUILD_HOST and BUILD_TIME for deterministic build (("-DBUILD_HOST=.*") "-DBUILD_HOST=\"\\\"guix\\\"\"") (("-DBUILD_TIME=.*") "-DBUILD_TIME=\"\\\"0\\\"\""))))) (build-system gnu-build-system) (arguments '(#:tests? #f ; no "check" target #:make-flags (list "CC=gcc" "all" (string-append "prefix=" (assoc-ref %outputs "out"))) #:phases (modify-phases %standard-phases (delete 'configure)))) (inputs `(("python-wrapper" ,python-wrapper) ("tbb" ,tbb) ("zlib" ,zlib))) (supported-systems '("x86_64-linux")) (home-page "http://bowtie-bio.sourceforge.net/index.shtml") (synopsis "Fast aligner for short nucleotide sequence reads") (description "Bowtie is a fast, memory-efficient short read aligner. It aligns short DNA sequences (reads) to the human genome at a rate of over 25 million 35-bp reads per hour. Bowtie indexes the genome with a Burrows-Wheeler index to keep its memory footprint small: typically about 2.2 GB for the human genome (2.9 GB for paired-end).") (license license:artistic2.0))) (define-public tophat (package (name "tophat") (version "2.1.1") (source (origin (method url-fetch) (uri (string-append "http://ccb.jhu.edu/software/tophat/downloads/tophat-" version ".tar.gz")) (sha256 (base32 "19add02kv2xhd6ihd779dr7x35ggym3jqr0m5c4315i1yfb0p11p")) (modules '((guix build utils))) (snippet '(begin ;; Remove bundled SeqAn and samtools (delete-file-recursively "src/SeqAn-1.4.2") (delete-file-recursively "src/samtools-0.1.18") #t)))) (build-system gnu-build-system) (arguments '(#:parallel-build? #f ; not supported #:phases (modify-phases %standard-phases (add-after 'set-paths 'hide-default-gcc (lambda* (#:key inputs #:allow-other-keys) (let ((gcc (assoc-ref inputs "gcc"))) ;; Remove the default GCC from CPLUS_INCLUDE_PATH to prevent ;; conflicts with the GCC 5 input. (setenv "CPLUS_INCLUDE_PATH" (string-join (delete (string-append gcc "/include/c++") (string-split (getenv "CPLUS_INCLUDE_PATH") #\:)) ":")) #t))) (add-after 'unpack 'use-system-samtools (lambda* (#:key inputs #:allow-other-keys) (substitute* "src/Makefile.in" (("(noinst_LIBRARIES = )\\$\\(SAMLIB\\)" _ prefix) prefix) (("\\$\\(SAMPROG\\): \\$\\(SAMLIB\\)") "") (("SAMPROG = samtools_0\\.1\\.18") "") (("\\$\\(samtools_0_1_18_SOURCES\\)") "") (("am__EXEEXT_1 = samtools_0\\.1\\.18\\$\\(EXEEXT\\)") "")) (substitute* '("src/common.cpp" "src/tophat.py") (("samtools_0.1.18") (which "samtools"))) (substitute* '("src/common.h" "src/bam2fastx.cpp") (("#include \"bam.h\"") "#include ") (("#include \"sam.h\"") "#include ")) (substitute* '("src/bwt_map.h" "src/map2gtf.h" "src/align_status.h") (("#include ") "#include ") (("#include ") "#include ")) #t))))) (native-inputs `(("gcc@5" ,gcc-5))) ;; doesn't build with later versions (inputs `(("boost" ,boost) ("bowtie" ,bowtie) ("ncurses" ,ncurses) ("perl" ,perl) ("python" ,python-2) ("samtools" ,samtools-0.1) ("seqan" ,seqan-1) ("zlib" ,zlib))) (home-page "https://ccb.jhu.edu/software/tophat/index.shtml") (synopsis "Spliced read mapper for RNA-Seq data") (description "TopHat is a fast splice junction mapper for nucleotide sequence reads produced by the RNA-Seq method. It aligns RNA-Seq reads to mammalian-sized genomes using the ultra high-throughput short read aligner Bowtie, and then analyzes the mapping results to identify splice junctions between exons.") ;; TopHat is released under the Boost Software License, Version 1.0 ;; See https://github.com/infphilo/tophat/issues/11#issuecomment-121589893 (license license:boost1.0))) (define-public bwa (package (name "bwa") (version "0.7.17") (source (origin (method url-fetch) (uri (string-append "https://github.com/lh3/bwa/releases/download/v" version "/bwa-" version ".tar.bz2")) (sha256 (base32 "1zfhv2zg9v1icdlq4p9ssc8k01mca5d1bd87w71py2swfi74s6yy")))) (build-system gnu-build-system) (arguments '(#:tests? #f ;no "check" target #:phases (modify-phases %standard-phases (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (lib (string-append out "/lib")) (doc (string-append out "/share/doc/bwa")) (man (string-append out "/share/man/man1"))) (install-file "bwa" bin) (install-file "libbwa.a" lib) (install-file "README.md" doc) (install-file "bwa.1" man)) #t)) ;; no "configure" script (delete 'configure)))) (inputs `(("zlib" ,zlib))) ;; Non-portable SSE instructions are used so building fails on platforms ;; other than x86_64. (supported-systems '("x86_64-linux")) (home-page "http://bio-bwa.sourceforge.net/") (synopsis "Burrows-Wheeler sequence aligner") (description "BWA is a software package for mapping low-divergent sequences against a large reference genome, such as the human genome. It consists of three algorithms: BWA-backtrack, BWA-SW and BWA-MEM. The first algorithm is designed for Illumina sequence reads up to 100bp, while the rest two for longer sequences ranged from 70bp to 1Mbp. BWA-MEM and BWA-SW share similar features such as long-read support and split alignment, but BWA-MEM, which is the latest, is generally recommended for high-quality queries as it is faster and more accurate. BWA-MEM also has better performance than BWA-backtrack for 70-100bp Illumina reads.") (license license:gpl3+))) (define-public bwa-pssm (package (inherit bwa) (name "bwa-pssm") (version "0.5.11") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/pkerpedjiev/bwa-pssm") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "076c4q0cdqz8jgylb067y9zmvxglppnzi3qiscn0xiypgc6lgb5r")))) (build-system gnu-build-system) (inputs `(("gdsl" ,gdsl) ("zlib" ,zlib) ("perl" ,perl))) (home-page "http://bwa-pssm.binf.ku.dk/") (synopsis "Burrows-Wheeler transform-based probabilistic short read mapper") (description "BWA-PSSM is a probabilistic short genomic sequence read aligner based on the use of @dfn{position specific scoring matrices} (PSSM). Like many of the existing aligners it is fast and sensitive. Unlike most other aligners, however, it is also adaptible in the sense that one can direct the alignment based on known biases within the data set. It is coded as a modification of the original BWA alignment program and shares the genome index structure as well as many of the command line options.") (license license:gpl3+))) (define-public bwa-meth (package (name "bwa-meth") (version "0.2.2") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/brentp/bwa-meth") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "17j31i7zws5j7mhsq9x3qgkxly6mlmrgwhfq0qbflgxrmx04yaiz")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'keep-references-to-bwa (lambda* (#:key inputs #:allow-other-keys) (substitute* "bwameth.py" (("bwa (mem|index)" _ command) (string-append (which "bwa") " " command)) ;; There's an ill-advised check for "samtools" on PATH. (("^checkX.*") "")) #t))))) (inputs `(("bwa" ,bwa))) (native-inputs `(("python-toolshed" ,python-toolshed))) (home-page "https://github.com/brentp/bwa-meth") (synopsis "Fast and accurante alignment of BS-Seq reads") (description "BWA-Meth works for single-end reads and for paired-end reads from the directional protocol (most common). It uses the method employed by methylcoder and Bismark of in silico conversion of all C's to T's in both reference and reads. It recovers the original read (needed to tabulate methylation) by attaching it as a comment which BWA appends as a tag to the read. It performs favorably to existing aligners gauged by number of on and off-target reads for a capture method that targets CpG-rich region.") (license license:expat))) (define-public python-bx-python (package (name "python-bx-python") (version "0.8.2") (source (origin (method url-fetch) (uri (pypi-uri "bx-python" version)) (sha256 (base32 "11kksg2rbzihpmcid823xvg42xi88m7sz58rzk29abybkxy0rszs")))) (build-system python-build-system) ;; Tests fail because test data are not included (arguments '(#:tests? #f)) (propagated-inputs `(("python-numpy" ,python-numpy) ("python-six" ,python-six))) (inputs `(("zlib" ,zlib))) (native-inputs `(("python-lzo" ,python-lzo) ("python-nose" ,python-nose) ("python-cython" ,python-cython))) (home-page "https://github.com/bxlab/bx-python") (synopsis "Tools for manipulating biological data") (description "bx-python provides tools for manipulating biological data, particularly multiple sequence alignments.") (license license:expat))) (define-public python2-bx-python (package-with-python2 python-bx-python)) (define-public python-pyega3 (package (name "python-pyega3") (version "3.4.1") (source (origin (method url-fetch) (uri (pypi-uri "pyega3" version)) (sha256 (base32 "1k736in8g27rarx65ym9xk50x53zjg75h37bb8ljynxv04rypx2q")))) (build-system python-build-system) (arguments `(#:tests? #f)) ; The tests require network access. (native-inputs `(("python-psutil" ,python-psutil) ("python-htsget" ,python-htsget))) (propagated-inputs `(("python-requests" ,python-requests) ("python-tqdm" ,python-tqdm) ("python-urllib3" ,python-urllib3) ("python-responses" ,python-responses))) (home-page "https://github.com/EGA-archive/ega-download-client") (synopsis "Python client for EGA") (description "This package is a python-based tool for viewing and downloading files from authorized EGA datasets. It uses the EGA data API and has several key features: @itemize @item Files are transferred over secure https connections and received unencrypted, so no need for decryption after download. @item Downloads resume from where they left off in the event that the connection is interrupted. @item Supports file segmenting and parallelized download of segments, improving overall performance. @item After download completes, file integrity is verified using checksums. @item Implements the GA4GH-compliant htsget protocol for download of genomic ranges for data files with accompanying index files. @end itemize\n") (license license:asl2.0))) (define-public python-pysam (package (name "python-pysam") (version "0.15.1") (source (origin (method git-fetch) ;; Test data is missing on PyPi. (uri (git-reference (url "https://github.com/pysam-developers/pysam") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1vj367w6xbn9bpmksm162l1aipf7cj97h1q83y7jcpm33ihwpf7x")) (modules '((guix build utils))) (snippet '(begin ;; Drop bundled htslib. TODO: Also remove samtools ;; and bcftools. (delete-file-recursively "htslib") #t)))) (build-system python-build-system) (arguments `(#:modules ((ice-9 ftw) (srfi srfi-26) (guix build python-build-system) (guix build utils)) #:phases (modify-phases %standard-phases (add-before 'build 'set-flags (lambda* (#:key inputs #:allow-other-keys) (setenv "HTSLIB_MODE" "external") (setenv "HTSLIB_LIBRARY_DIR" (string-append (assoc-ref inputs "htslib") "/lib")) (setenv "HTSLIB_INCLUDE_DIR" (string-append (assoc-ref inputs "htslib") "/include")) (setenv "LDFLAGS" "-lncurses") (setenv "CFLAGS" "-D_CURSES_LIB=1") #t)) (replace 'check (lambda* (#:key inputs outputs #:allow-other-keys) ;; This file contains tests that require a connection to the ;; internet. (delete-file "tests/tabix_test.py") ;; FIXME: This test fails (delete-file "tests/AlignmentFile_test.py") ;; Add first subdirectory of "build" directory to PYTHONPATH. (setenv "PYTHONPATH" (string-append (getenv "PYTHONPATH") ":" (getcwd) "/build/" (car (scandir "build" (negate (cut string-prefix? "." <>)))))) ;; Step out of source dir so python does not import from CWD. (with-directory-excursion "tests" (setenv "HOME" "/tmp") (invoke "make" "-C" "pysam_data") (invoke "make" "-C" "cbcf_data") ;; Running nosetests without explicitly asking for a single ;; process leads to a crash. Running with multiple processes ;; fails because the tests are not designed to run in parallel. ;; FIXME: tests keep timing out on some systems. (invoke "nosetests" "-v" "--processes" "1"))))))) (propagated-inputs `(("htslib" ,htslib-1.9))) ; Included from installed header files. (inputs `(("ncurses" ,ncurses) ("curl" ,curl) ("zlib" ,zlib))) (native-inputs `(("python-cython" ,python-cython) ;; Dependencies below are are for tests only. ("samtools" ,samtools-1.9) ("bcftools" ,bcftools-1.9) ("python-nose" ,python-nose))) (home-page "https://github.com/pysam-developers/pysam") (synopsis "Python bindings to the SAMtools C API") (description "Pysam is a Python module for reading and manipulating files in the SAM/BAM format. Pysam is a lightweight wrapper of the SAMtools C API. It also includes an interface for tabix.") (license license:expat))) (define-public python2-pysam (package-with-python2 python-pysam)) (define-public python-twobitreader (package (name "python-twobitreader") (version "3.1.6") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/benjschiller/twobitreader") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1qbxvv1h58cismbk1anpjrkpghsaiy64a11ir3lhy6qch6xf8n62")))) (build-system python-build-system) ;; Tests are not included (arguments '(#:tests? #f)) (native-inputs `(("python-sphinx" ,python-sphinx))) (home-page "https://github.com/benjschiller/twobitreader") (synopsis "Python library for reading .2bit files") (description "twobitreader is a Python library for reading .2bit files as used by the UCSC genome browser.") (license license:artistic2.0))) (define-public python2-twobitreader (package-with-python2 python-twobitreader)) (define-public python-plastid (package (name "python-plastid") (version "0.4.8") (source (origin (method url-fetch) (uri (pypi-uri "plastid" version)) (sha256 (base32 "0l24dd3q66if8yj042m4s0g95n6acn7im1imqd3p6h8ns43kxhj8")))) (build-system python-build-system) (arguments ;; Some test files are not included. `(#:tests? #f)) (propagated-inputs `(("python-numpy" ,python-numpy) ("python-scipy" ,python-scipy) ("python-pandas" ,python-pandas) ("python-pysam" ,python-pysam) ("python-matplotlib" ,python-matplotlib) ("python-biopython" ,python-biopython) ("python-twobitreader" ,python-twobitreader) ("python-termcolor" ,python-termcolor))) (native-inputs `(("python-cython" ,python-cython) ("python-nose" ,python-nose))) (home-page "https://github.com/joshuagryphon/plastid") (synopsis "Python library for genomic analysis") (description "plastid is a Python library for genomic analysis – in particular, high-throughput sequencing data – with an emphasis on simplicity.") (license license:bsd-3))) (define-public python2-plastid (package-with-python2 python-plastid)) (define-public tetoolkit (package (name "tetoolkit") (version "2.0.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/mhammell-laboratory/tetoolkit") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1yzi0kfpzip8zpjb82x1ik6h22yzfyjiz2dv85v6as2awwqvk807")))) (build-system python-build-system) (arguments `(#:python ,python-2 ; not guaranteed to work with Python 3 #:phases (modify-phases %standard-phases (add-after 'unpack 'make-writable (lambda _ (for-each make-file-writable (find-files ".")) #t)) (add-after 'unpack 'patch-invocations (lambda* (#:key inputs #:allow-other-keys) (substitute* '("bin/TEtranscripts" "bin/TEcount") (("'sort ") (string-append "'" (which "sort") " ")) (("'rm -f ") (string-append "'" (which "rm") " -f ")) (("'Rscript'") (string-append "'" (which "Rscript") "'"))) (substitute* "TEToolkit/IO/ReadInputs.py" (("BamToBED") (which "bamToBed"))) (substitute* "TEToolkit/Normalization.py" (("\"Rscript\"") (string-append "\"" (which "Rscript") "\""))) #t)) (add-after 'install 'wrap-program (lambda* (#:key outputs #:allow-other-keys) ;; Make sure the executables find R packages. (let ((out (assoc-ref outputs "out"))) (for-each (lambda (script) (wrap-program (string-append out "/bin/" script) `("R_LIBS_SITE" ":" = (,(getenv "R_LIBS_SITE"))))) '("TEtranscripts" "TEcount"))) #t))))) (inputs `(("coreutils" ,coreutils) ("bedtools" ,bedtools) ("python-argparse" ,python2-argparse) ("python-pysam" ,python2-pysam) ("r-minimal" ,r-minimal) ("r-deseq2" ,r-deseq2))) (home-page "https://github.com/mhammell-laboratory/tetoolkit") (synopsis "Transposable elements in differential enrichment analysis") (description "This is package for including transposable elements in differential enrichment analysis of sequencing datasets. TEtranscripts and TEcount take RNA-seq (and similar data) and annotates reads to both genes and transposable elements. TEtranscripts then performs differential analysis using DESeq2. Note that TEtranscripts and TEcount rely on specially curated GTF files, which are not included due to their size.") (license license:gpl3+))) (define-public cd-hit (package (name "cd-hit") (version "4.6.8") (source (origin (method url-fetch) (uri (string-append "https://github.com/weizhongli/cdhit" "/releases/download/V" version "/cd-hit-v" version "-2017-0621-source.tar.gz")) (sha256 (base32 "1b4mwm2520ixjbw57sil20f9iixzw4bkdqqwgg1fc3pzm6rz4zmn")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are no tests #:make-flags ;; Executables are copied directly to the PREFIX. (list (string-append "PREFIX=" (assoc-ref %outputs "out") "/bin") ;; Support longer sequences (e.g. Pacbio sequences) "MAX_SEQ=60000000") #:phases (modify-phases %standard-phases ;; No "configure" script (delete 'configure) ;; Remove sources of non-determinism (add-after 'unpack 'be-timeless (lambda _ (substitute* "cdhit-utility.c++" ((" \\(built on \" __DATE__ \"\\)") "")) (substitute* "cdhit-common.c++" (("__DATE__") "\"0\"") (("\", %s, \" __TIME__ \"\\\\n\", date") "")) #t)) ;; The "install" target does not create the target directory. (add-before 'install 'create-target-dir (lambda* (#:key outputs #:allow-other-keys) (mkdir-p (string-append (assoc-ref outputs "out") "/bin")) #t))))) (inputs `(("perl" ,perl))) (home-page "http://weizhongli-lab.org/cd-hit/") (synopsis "Cluster and compare protein or nucleotide sequences") (description "CD-HIT is a program for clustering and comparing protein or nucleotide sequences. CD-HIT is designed to be fast and handle extremely large databases.") ;; The manual says: "It can be copied under the GNU General Public License ;; version 2 (GPLv2)." (license license:gpl2))) (define-public clipper (package (name "clipper") (version "2.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/YeoLab/clipper") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1bcag4lb5bkzsj2vg7lrq24aw6yfgq275ifrbhd82l7kqgbbjbkv")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-before 'reset-gzip-timestamps 'make-files-writable (lambda* (#:key outputs #:allow-other-keys) ;; Make sure .gz files are writable so that the ;; 'reset-gzip-timestamps' phase can do its work. (let ((out (assoc-ref outputs "out"))) (for-each make-file-writable (find-files out "\\.gz$")) #t)))))) (inputs `(("htseq" ,htseq) ("python-pybedtools" ,python-pybedtools) ("python-cython" ,python-cython) ("python-scikit-learn" ,python-scikit-learn) ("python-matplotlib" ,python-matplotlib) ("python-pandas" ,python-pandas) ("python-pysam" ,python-pysam) ("python-numpy" ,python-numpy) ("python-scipy" ,python-scipy))) (native-inputs `(("python-setuptools-git" ,python-setuptools-git) ("python-mock" ,python-mock) ; for tests ("python-nose" ,python-nose) ; for tests ("python-pytz" ,python-pytz))) ; for tests (home-page "https://github.com/YeoLab/clipper") (synopsis "CLIP peak enrichment recognition") (description "CLIPper is a tool to define peaks in CLIP-seq datasets.") (license license:gpl2))) (define-public codingquarry (package (name "codingquarry") (version "2.0") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/codingquarry/CodingQuarry_v" version ".tar.gz")) (sha256 (base32 "0115hkjflsnfzn36xppwf9h9avfxlavr43djqmshkkzbgjzsz60i")))) (build-system gnu-build-system) (arguments '(#:tests? #f ; no "check" target #:phases (modify-phases %standard-phases (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (doc (string-append out "/share/doc/codingquarry"))) (install-file "INSTRUCTIONS.pdf" doc) (copy-recursively "QuarryFiles" (string-append out "/QuarryFiles")) (install-file "CodingQuarry" bin) (install-file "CufflinksGTF_to_CodingQuarryGFF3.py" bin)) #t))))) (inputs `(("openmpi" ,openmpi))) (native-search-paths (list (search-path-specification (variable "QUARRY_PATH") (files '("QuarryFiles"))))) (native-inputs `(("python" ,python-2))) ; Only Python 2 is supported (synopsis "Fungal gene predictor") (description "CodingQuarry is a highly accurate, self-training GHMM fungal gene predictor designed to work with assembled, aligned RNA-seq transcripts.") (home-page "https://sourceforge.net/projects/codingquarry/") (license license:gpl3+))) (define-public couger (package (name "couger") (version "1.8.2") (source (origin (method url-fetch) (uri (string-append "http://couger.oit.duke.edu/static/assets/COUGER" version ".zip")) (sha256 (base32 "04p2b14nmhzxw5h72mpzdhalv21bx4w9b87z0wpw0xzxpysyncmq")))) (build-system gnu-build-system) (arguments `(#:tests? #f #:phases (modify-phases %standard-phases (delete 'configure) (delete 'build) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin"))) (copy-recursively "src" (string-append out "/src")) (mkdir bin) ;; Add "src" directory to module lookup path. (substitute* "couger" (("from argparse") (string-append "import sys\nsys.path.append(\"" out "\")\nfrom argparse"))) (install-file "couger" bin)) #t)) (add-after 'install 'wrap-program (lambda* (#:key inputs outputs #:allow-other-keys) ;; Make sure 'couger' runs with the correct PYTHONPATH. (let* ((out (assoc-ref outputs "out")) (path (getenv "PYTHONPATH"))) (wrap-program (string-append out "/bin/couger") `("PYTHONPATH" ":" prefix (,path)))) #t))))) (inputs `(("python" ,python-2) ("python2-pillow" ,python2-pillow) ("python2-numpy" ,python2-numpy) ("python2-scipy" ,python2-scipy) ("python2-matplotlib" ,python2-matplotlib))) (propagated-inputs `(("r-minimal" ,r-minimal) ("libsvm" ,libsvm) ("randomjungle" ,randomjungle))) (native-inputs `(("unzip" ,unzip))) (home-page "http://couger.oit.duke.edu") (synopsis "Identify co-factors in sets of genomic regions") (description "COUGER can be applied to any two sets of genomic regions bound by paralogous TFs (e.g., regions derived from ChIP-seq experiments) to identify putative co-factors that provide specificity to each TF. The framework determines the genomic targets uniquely-bound by each TF, and identifies a small set of co-factors that best explain the in vivo binding differences between the two TFs. COUGER uses classification algorithms (support vector machines and random forests) with features that reflect the DNA binding specificities of putative co-factors. The features are generated either from high-throughput TF-DNA binding data (from protein binding microarray experiments), or from large collections of DNA motifs.") (license license:gpl3+))) (define-public clustal-omega (package (name "clustal-omega") (version "1.2.4") (source (origin (method url-fetch) (uri (string-append "http://www.clustal.org/omega/clustal-omega-" version ".tar.gz")) (sha256 (base32 "1vm30mzncwdv881vrcwg11vzvrsmwy4wg80j5i0lcfk6dlld50w6")))) (build-system gnu-build-system) (inputs `(("argtable" ,argtable))) (home-page "http://www.clustal.org/omega/") (synopsis "Multiple sequence aligner for protein and DNA/RNA") (description "Clustal-Omega is a general purpose multiple sequence alignment (MSA) program for protein and DNA/RNA. It produces high quality MSAs and is capable of handling data-sets of hundreds of thousands of sequences in reasonable time.") (license license:gpl2+))) (define-public crossmap (package (name "crossmap") (version "0.3.8") (source (origin (method url-fetch) (uri (pypi-uri "CrossMap" version)) (sha256 (base32 "1sb2f2qbxya4fzw3yjl09vbrs8vfmw22zrygrvz004sf9gb1vkan")))) (build-system python-build-system) (inputs `(("python-bx-python" ,python-bx-python) ("python-numpy" ,python-numpy) ("python-pybigwig" ,python-pybigwig) ("python-pysam" ,python-pysam) ("zlib" ,zlib))) (native-inputs `(("python-cython" ,python-cython) ("python-nose" ,python-nose))) (home-page "http://crossmap.sourceforge.net/") (synopsis "Convert genome coordinates between assemblies") (description "CrossMap is a program for conversion of genome coordinates or annotation files between different genome assemblies. It supports most commonly used file formats including SAM/BAM, Wiggle/BigWig, BED, GFF/GTF, VCF.") (license license:gpl2+))) (define-public python-dnaio (package (name "python-dnaio") (version "0.3") (source (origin (method url-fetch) (uri (pypi-uri "dnaio" version)) (sha256 (base32 "0f16m7hdlm0fz1n7y5asy0v9ghyrq17ni1p9iybq22ddzyd49r27")))) (build-system python-build-system) (native-inputs `(("python-cython" ,python-cython) ("python-pytest" ,python-pytest) ("python-xopen" ,python-xopen))) (home-page "https://github.com/marcelm/dnaio/") (synopsis "Read FASTA and FASTQ files efficiently") (description "dnaio is a Python library for fast parsing of FASTQ and also FASTA files. The code was previously part of the cutadapt tool.") (license license:expat))) (define-public python-deeptoolsintervals (package (name "python-deeptoolsintervals") (version "0.1.9") (source (origin (method url-fetch) (uri (pypi-uri "deeptoolsintervals" version)) (sha256 (base32 "1xnl80nblysj6dylj4683wgrfa425rkx4dp5k65hvwdns9pw753x")))) (build-system python-build-system) (inputs `(("zlib" ,zlib))) (home-page "https://github.com/deeptools/deeptools_intervals") (synopsis "Create GTF-based interval trees with associated meta-data") (description "This package provides a Python module creating/accessing GTF-based interval trees with associated meta-data. It is primarily used by the @code{deeptools} package.") (license license:expat))) (define-public python-deeptools (package (name "python-deeptools") (version "3.4.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/deeptools/deepTools") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "0l09vyynz6s6w7fnyd94rpys4a6aja6kp4gli64pngdxdz3md1nl")))) (build-system python-build-system) (native-inputs `(("python-mock" ,python-mock) ("python-nose" ,python-nose))) (propagated-inputs `(("python-matplotlib" ,python-matplotlib) ("python-numpy" ,python-numpy) ("python-numpydoc" ,python-numpydoc) ("python-py2bit" ,python-py2bit) ("python-pybigwig" ,python-pybigwig) ("python-pysam" ,python-pysam) ("python-scipy" ,python-scipy) ("python-deeptoolsintervals" ,python-deeptoolsintervals) ("python-plotly" ,python-plotly-2.4.1))) (home-page "https://pypi.org/project/deepTools/") (synopsis "Useful tools for exploring deep sequencing data") (description "This package addresses the challenge of handling large amounts of data that are now routinely generated from DNA sequencing centers. @code{deepTools} contains useful modules to process the mapped reads data for multiple quality checks, creating normalized coverage files in standard bedGraph and bigWig file formats, that allow comparison between different files. Finally, using such normalized and standardized files, deepTools can create many publication-ready visualizations to identify enrichments and for functional annotations of the genome.") ;; The file deeptools/cm.py is licensed under the BSD license. The ;; remainder of the code is licensed under the MIT license. (license (list license:bsd-3 license:expat)))) (define-deprecated deeptools python-deeptools) (define-public cutadapt (package (name "cutadapt") (version "2.1") (source (origin (method url-fetch) (uri (pypi-uri "cutadapt" version)) (sha256 (base32 "1vqmsfkm6llxzmsz9wcfcvzx9a9f8iabvwik2rbyn7nc4wm25z89")))) (build-system python-build-system) (inputs `(("python-dnaio" ,python-dnaio) ("python-xopen" ,python-xopen))) (native-inputs `(("python-cython" ,python-cython) ("python-pytest" ,python-pytest) ("python-setuptools-scm" ,python-setuptools-scm))) (home-page "https://cutadapt.readthedocs.io/en/stable/") (synopsis "Remove adapter sequences from nucleotide sequencing reads") (description "Cutadapt finds and removes adapter sequences, primers, poly-A tails and other types of unwanted sequence from high-throughput sequencing reads.") (license license:expat))) (define-public libbigwig (package (name "libbigwig") (version "0.4.4") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/dpryan79/libBigWig") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "09693dmf1scdac5pyq6qyn8b4mcipvnmc370k9a5z41z81m3dcsj")))) (build-system gnu-build-system) (arguments `(#:test-target "test" #:tests? #f ; tests require access to the web #:make-flags (list "CC=gcc" (string-append "prefix=" (assoc-ref %outputs "out"))) #:phases (modify-phases %standard-phases (delete 'configure)))) (inputs `(("zlib" ,zlib) ("curl" ,curl))) (native-inputs `(("doxygen" ,doxygen) ;; Need for tests ("python" ,python-2))) (home-page "https://github.com/dpryan79/libBigWig") (synopsis "C library for handling bigWig files") (description "This package provides a C library for parsing local and remote BigWig files.") (license license:expat))) (define-public python-pybigwig (package (name "python-pybigwig") (version "0.3.17") (source (origin (method url-fetch) (uri (pypi-uri "pyBigWig" version)) (sha256 (base32 "157x6v48y299zm382krf1dw08fdxg95im8lnabhp5vc94s04zxj1")) (modules '((guix build utils))) (snippet '(begin ;; Delete bundled libBigWig sources (delete-file-recursively "libBigWig") #t)))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'link-with-libBigWig (lambda* (#:key inputs #:allow-other-keys) (substitute* "setup.py" (("libs=\\[") "libs=[\"BigWig\", ")) #t))))) (propagated-inputs `(("python-numpy" ,python-numpy))) (inputs `(("libbigwig" ,libbigwig) ("zlib" ,zlib) ("curl" ,curl))) (home-page "https://github.com/dpryan79/pyBigWig") (synopsis "Access bigWig files in Python using libBigWig") (description "This package provides Python bindings to the libBigWig library for accessing bigWig files.") (license license:expat))) (define-public python2-pybigwig (package-with-python2 python-pybigwig)) (define-public python-schema-salad (package (name "python-schema-salad") (version "7.1.20210316164414") (source (origin (method url-fetch) (uri (pypi-uri "schema-salad" version)) (sha256 (base32 "04jaykdpgfnkrghvli5swxzqp7yba842am4bz42hcfljsmkrxvrk")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-before 'check 'skip-failing-tests (lambda _ ;; Skip tests that require network access. (substitute* "schema_salad/tests/test_cwl11.py" (("^def test_(secondaryFiles|outputBinding)" all) (string-append "@pytest.mark.skip(reason=" "\"test requires network access\")\n" all))) #t))))) (propagated-inputs `(("python-cachecontrol" ,python-cachecontrol-0.11) ("python-lockfile" ,python-lockfile) ("python-mistune" ,python-mistune) ("python-rdflib" ,python-rdflib) ("python-rdflib-jsonld" ,python-rdflib-jsonld) ("python-requests" ,python-requests) ("python-ruamel.yaml" ,python-ruamel.yaml) ("python-typing-extensions" ,python-typing-extensions))) (native-inputs `(("python-pytest" ,python-pytest) ("python-pytest-runner" ,python-pytest-runner))) (home-page "https://github.com/common-workflow-language/schema_salad") (synopsis "Schema Annotations for Linked Avro Data (SALAD)") (description "Salad is a schema language for describing JSON or YAML structured linked data documents. Salad schema describes rules for preprocessing, structural validation, and hyperlink checking for documents described by a Salad schema. Salad supports rich data modeling with inheritance, template specialization, object identifiers, object references, documentation generation, code generation, and transformation to RDF. Salad provides a bridge between document and record oriented data modeling and the Semantic Web.") (license license:asl2.0))) (define-public cwltool (package (name "cwltool") (version "3.0.20210319143721") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/common-workflow-language/cwltool") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1sgs9ckyxb9f9169mc3wm9lnjg4080ai42xqsrwpw9l8apy4c9m5")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'loosen-version-restrictions (lambda _ (substitute* "setup.py" (("== 1.5.1") ">=1.5.1") ; prov ((", < 3.5") "") ; shellescape ((" >= 6.0.2, < 6.2") "")) ; pytest #t)) (add-after 'unpack 'dont-use-git (lambda _ (substitute* "gittaggers.py" (("self.git_timestamp_tag\\(\\)") (string-append "time.strftime('.%Y%m%d%H%M%S', time.gmtime(int(" (string-drop ,version 4) ")))"))) #t)) (add-after 'unpack 'modify-tests (lambda _ ;; Tries to connect to the internet. (delete-file "tests/test_content_type.py") (delete-file "tests/test_udocker.py") (delete-file "tests/test_http_input.py") (substitute* "tests/test_load_tool.py" (("def test_load_graph_fragment_from_packed") (string-append "@pytest.mark.skip(reason=\"Disabled by Guix\")\n" "def test_load_graph_fragment_from_packed"))) (substitute* "tests/test_examples.py" (("def test_env_filtering") (string-append "@pytest.mark.skip(reason=\"Disabled by Guix\")\n" "def test_env_filtering"))) ;; Tries to use cwl-runners. (substitute* "tests/test_examples.py" (("def test_v1_0_arg_empty_prefix_separate_false") (string-append "@pytest.mark.skip(reason=\"Disabled by Guix\")\n" "def test_v1_0_arg_empty_prefix_separate_false"))) #t))))) (propagated-inputs `(("python-argcomplete" ,python-argcomplete) ("python-bagit" ,python-bagit) ("python-coloredlogs" ,python-coloredlogs) ("python-mypy-extensions" ,python-mypy-extensions) ("python-prov" ,python-prov) ("python-pydot" ,python-pydot) ("python-psutil" ,python-psutil) ("python-rdflib" ,python-rdflib) ("python-requests" ,python-requests) ("python-ruamel.yaml" ,python-ruamel.yaml) ("python-schema-salad" ,python-schema-salad) ("python-shellescape" ,python-shellescape) ("python-typing-extensions" ,python-typing-extensions) ;; Not listed as needed but still necessary: ("node" ,node))) (native-inputs `(("python-arcp" ,python-arcp) ("python-humanfriendly" ,python-humanfriendly) ("python-mock" ,python-mock) ("python-pytest" ,python-pytest) ("python-pytest-cov" ,python-pytest-cov) ("python-pytest-mock" ,python-pytest-mock) ("python-pytest-runner" ,python-pytest-runner) ("python-rdflib-jsonld" ,python-rdflib-jsonld))) (home-page "https://github.com/common-workflow-language/common-workflow-language") (synopsis "Common Workflow Language reference implementation") (description "This is the reference implementation of the @acronym{CWL, Common Workflow Language} standards. The CWL open standards are for describing analysis workflows and tools in a way that makes them portable and scalable across a variety of software and hardware environments, from workstations to cluster, cloud, and high performance computing (HPC) environments. CWL is designed to meet the needs of data-intensive science, such as Bioinformatics, Medical Imaging, Astronomy, Physics, and Chemistry. The @acronym{cwltool, CWL reference implementation} is intended to be feature complete and to provide comprehensive validation of CWL files as well as provide other tools related to working with CWL descriptions.") (license license:asl2.0))) (define-public python-dendropy (package (name "python-dendropy") (version "4.4.0") (source (origin (method git-fetch) ;; Source from GitHub so that tests are included. (uri (git-reference (url "https://github.com/jeetsukumaran/DendroPy") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "097hfyv2kaf4x92i4rjx0paw2cncxap48qivv8zxng4z7nhid0x9")))) (build-system python-build-system) (home-page "https://dendropy.org/") (synopsis "Library for phylogenetics and phylogenetic computing") (description "DendroPy is a library for phylogenetics and phylogenetic computing: reading, writing, simulation, processing and manipulation of phylogenetic trees (phylogenies) and characters.") (license license:bsd-3))) (define-public python2-dendropy (let ((base (package-with-python2 python-dendropy))) (package/inherit base (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'remove-failing-test (lambda _ ;; This test fails when the full test suite is run, as documented ;; at https://github.com/jeetsukumaran/DendroPy/issues/74 (substitute* "tests/test_dataio_nexml_reader_tree_list.py" (("test_collection_comments_and_annotations") "do_not_test_collection_comments_and_annotations")) #t))) ,@(package-arguments base)))))) (define-public python-py2bit (package (name "python-py2bit") (version "0.3.0") (source (origin (method url-fetch) (uri (pypi-uri "py2bit" version)) (sha256 (base32 "1vw2nvw1yrl7ikkqsqs1pg239yr5nspvd969r1x9arms1k25a1a5")))) (build-system python-build-system) (home-page "https://github.com/dpryan79/py2bit") (synopsis "Access 2bit files using lib2bit") (description "This package provides Python bindings for lib2bit to access 2bit files with Python.") (license license:expat))) (define-public delly (package (name "delly") (version "0.8.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/dellytools/delly") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1ibnplgfzj96w8glkx17v7sld3pm402fr5ybmf3h0rlcryabxrqy")) (modules '((guix build utils))) (snippet '(begin (delete-file-recursively "src/htslib") #t)))) (build-system gnu-build-system) (arguments `(#:tests? #f ; There are no tests to run. #:make-flags (list "PARALLEL=1" ; Allow parallel execution at run-time. (string-append "prefix=" (assoc-ref %outputs "out"))) #:phases (modify-phases %standard-phases (delete 'configure) ; There is no configure phase. (add-after 'install 'install-templates (lambda* (#:key outputs #:allow-other-keys) (let ((templates (string-append (assoc-ref outputs "out") "/share/delly/templates"))) (mkdir-p templates) (copy-recursively "excludeTemplates" templates) #t)))))) (inputs `(("boost" ,boost) ("bzip2" ,bzip2) ("htslib" ,htslib) ("zlib" ,zlib))) (home-page "https://github.com/dellytools/delly") (synopsis "Integrated structural variant prediction method") (description "Delly is an integrated structural variant prediction method that can discover and genotype deletions, tandem duplications, inversions and translocations at single-nucleotide resolution in short-read massively parallel sequencing data. It uses paired-ends and split-reads to sensitively and accurately delineate genomic rearrangements throughout the genome.") (license license:gpl3+))) (define-public trf (package (name "trf") (version "4.09.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/Benson-Genomics-Lab/TRF") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0fhwr4s1mf8nw8fr5imwjvjr42b59p97zr961ifm8xl1bajz4wpg")))) (build-system gnu-build-system) (home-page "https://github.com/Benson-Genomics-Lab/TRF") (synopsis "Tandem Repeats Finder: a program to analyze DNA sequences") (description "A tandem repeat in DNA is two or more adjacent, approximate copies of a pattern of nucleotides. Tandem Repeats Finder is a program to locate and display tandem repeats in DNA sequences. In order to use the program, the user submits a sequence in FASTA format. The output consists of two files: a repeat table file and an alignment file. Submitted sequences may be of arbitrary length. Repeats with pattern size in the range from 1 to 2000 bases are detected.") (license license:agpl3+))) (define-public repeat-masker (package (name "repeat-masker") (version "4.1.1") (source (origin (method url-fetch) (uri (string-append "http://www.repeatmasker.org/" "RepeatMasker/RepeatMasker-" version ".tar.gz")) (sha256 (base32 "03144sl9kh5ni2i33phi7x2pjndzbm5bjw3r4kqvmm6hxyb4k4x2")))) (build-system gnu-build-system) (arguments `(#:tests? #false ; there are none #:phases (modify-phases %standard-phases (delete 'configure) (replace 'build (lambda* (#:key inputs outputs #:allow-other-keys) (let ((share (string-append (assoc-ref outputs "out") "/share/RepeatMasker"))) (mkdir-p share) (copy-recursively "." share) (with-directory-excursion share (invoke "perl" "configure" "--trf_prgm" (which "trf") "--hmmer_dir" (string-append (assoc-ref inputs "hmmer") "/bin")))))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (share (string-append out "/share/RepeatMasker")) (bin (string-append out "/bin")) (path (getenv "PERL5LIB"))) (install-file (string-append share "/RepeatMasker") bin) (wrap-program (string-append bin "/RepeatMasker") `("PERL5LIB" ":" prefix (,path ,share))))))))) (inputs `(("perl" ,perl) ("perl-text-soundex" ,perl-text-soundex) ("python" ,python) ("python-h5py" ,python-h5py) ("hmmer" ,hmmer) ("trf" ,trf))) (home-page "https://github.com/Benson-Genomics-Lab/TRF") (synopsis "Tandem Repeats Finder: a program to analyze DNA sequences") (description "A tandem repeat in DNA is two or more adjacent, approximate copies of a pattern of nucleotides. Tandem Repeats Finder is a program to locate and display tandem repeats in DNA sequences. In order to use the program, the user submits a sequence in FASTA format. The output consists of two files: a repeat table file and an alignment file. Submitted sequences may be of arbitrary length. Repeats with pattern size in the range from 1 to 2000 bases are detected.") (license license:osl2.1))) (define-public diamond (package (name "diamond") (version "0.9.30") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/bbuchfink/diamond") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0k6f3kb6cniw11xw6763kkbs1sl0yack7xsy7q5fl5v170ssphq4")))) (build-system cmake-build-system) (arguments '(#:tests? #f ; no "check" target #:phases (modify-phases %standard-phases (add-after 'unpack 'remove-native-compilation (lambda _ (substitute* "CMakeLists.txt" (("-march=native") "")) #t))))) (inputs `(("zlib" ,zlib))) (home-page "https://github.com/bbuchfink/diamond") (synopsis "Accelerated BLAST compatible local sequence aligner") (description "DIAMOND is a BLAST-compatible local aligner for mapping protein and translated DNA query sequences against a protein reference database (BLASTP and BLASTX alignment mode). The speedup over BLAST is up to 20,000 on short reads at a typical sensitivity of 90-99% relative to BLAST depending on the data and settings.") (license license:agpl3+))) (define-public discrover (package (name "discrover") (version "1.6.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/maaskola/discrover") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "173fwi2vb6a5kp406hm3jj6j7v4whww796f2qcygp4rpvamh307y")))) (build-system cmake-build-system) (arguments `(#:tests? #f ; there are no tests #:phases (modify-phases %standard-phases (add-after 'unpack 'fix-latex-errors (lambda _ (with-fluids ((%default-port-encoding #f)) (substitute* "doc/references.bib" (("\\{S\\}illanp[^,]+,") "{S}illanp{\\\"a}{\\\"a},"))) ;; XXX: I just can't get pdflatex to not complain about these ;; characters. They end up in the manual via the generated ;; discrover-cli-help.txt. (substitute* "src/hmm/cli.cpp" (("µ") "mu") (("η") "eta") (("≤") "<=")) ;; This seems to be a syntax error. (substitute* "doc/discrover-manual.tex" (("theverbbox\\[t\\]") "theverbbox")) #t)) (add-after 'unpack 'add-missing-includes (lambda _ (substitute* "src/executioninformation.hpp" (("#define EXECUTIONINFORMATION_HPP" line) (string-append line "\n#include "))) (substitute* "src/plasma/fasta.hpp" (("#define FASTA_HPP" line) (string-append line "\n#include "))) #t)) ;; FIXME: this is needed because we're using texlive-union, which ;; doesn't handle fonts correctly. It expects to be able to generate ;; fonts in the home directory. (add-before 'build 'setenv-HOME (lambda _ (setenv "HOME" "/tmp") #t))))) (inputs `(("boost" ,boost) ("cairo" ,cairo) ("rmath-standalone" ,rmath-standalone))) (native-inputs `(("texlive" ,(texlive-union (list texlive-fonts-cm texlive-fonts-amsfonts texlive-latex-doi texlive-latex-examplep texlive-latex-hyperref texlive-latex-ms texlive-latex-natbib texlive-bibtex ; style files used by natbib texlive-latex-pgf ; tikz texlive-latex-verbatimbox))) ("imagemagick" ,imagemagick))) (home-page "https://dorina.mdc-berlin.de/public/rajewsky/discrover/") (synopsis "Discover discriminative nucleotide sequence motifs") (description "Discrover is a motif discovery method to find binding sites of nucleic acid binding proteins.") (license license:gpl3+))) (define-public eigensoft (package (name "eigensoft") (version "7.2.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/DReichLab/EIG") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1c141fqvhnzibmnf22sv23vbmzm20kjjyrib44cfh75wyndp2d9k")) (modules '((guix build utils))) ;; Remove pre-built binaries. (snippet '(begin (delete-file-recursively "bin") (mkdir "bin") #t)))) (build-system gnu-build-system) (arguments `(#:tests? #f ; There are no tests. #:make-flags '("CC=gcc") #:phases (modify-phases %standard-phases ;; There is no configure phase, but the Makefile is in a ;; sub-directory. (replace 'configure (lambda _ (chdir "src") #t)) ;; The provided install target only copies executables to ;; the "bin" directory in the build root. (add-after 'install 'actually-install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin"))) (for-each (lambda (file) (install-file file bin)) (find-files "../bin" ".*")) #t)))))) (inputs `(("gsl" ,gsl) ("lapack" ,lapack) ("openblas" ,openblas) ("perl" ,perl) ("gfortran" ,gfortran "lib"))) (home-page "https://github.com/DReichLab/EIG") (synopsis "Tools for population genetics") (description "The EIGENSOFT package provides tools for population genetics and stratification correction. EIGENSOFT implements methods commonly used in population genetics analyses such as PCA, computation of Tracy-Widom statistics, and finding related individuals in structured populations. It comes with a built-in plotting script and supports multiple file formats and quantitative phenotypes.") ;; The license of the eigensoft tools is Expat, but since it's ;; linking with the GNU Scientific Library (GSL) the effective ;; license is the GPL. (license license:gpl3+))) (define-public edirect (package (name "edirect") (version "13.3.20200128") (source (origin (method url-fetch) (uri (string-append "ftp://ftp.ncbi.nlm.nih.gov/entrez/entrezdirect" "/versions/" version "/edirect-" version ".tar.gz")) (sha256 (base32 "093zp7klv81ph0y8mm8d78a9hnpfxbv2kdym70gzdf3vz176rw33")) (modules '((guix build utils))) (snippet '(begin (delete-file "Mozilla-CA.tar.gz") (substitute* "rchive.go" ;; This go library does not have any license. (("github.com/fiam/gounidecode/unidecode") "golang.org/rainycape/unidecode")) #t)))) (build-system perl-build-system) (arguments `(#:phases (modify-phases %standard-phases (delete 'configure) (delete 'build) (delete 'check) ; simple check after install (add-after 'unpack 'patch-programs (lambda* (#:key inputs #:allow-other-keys) ;; Ignore errors about missing xtract.Linux and rchive.Linux. (substitute* "pm-refresh" (("cat \\\"\\$target") "grep ^[[:digit:]] \"$target")) #t)) (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin")) (edirect-go (assoc-ref inputs "edirect-go-programs"))) (for-each (lambda (file) (install-file file bin)) '("archive-pubmed" "asp-cp" "asp-ls" "download-ncbi-data" "download-pubmed" "edirect.pl" "efetch" "epost" "esearch" "fetch-pubmed" "ftp-cp" "ftp-ls" "has-asp" "index-pubmed" "pm-prepare" "pm-refresh" "pm-stash" "pm-collect" "pm-index" "pm-invert" "pm-merge" "pm-promote")) (symlink (string-append edirect-go "/bin/xtract.Linux") (string-append bin "/xtract")) (symlink (string-append edirect-go "/bin/rchive.Linux") (string-append bin "/rchive"))) #t)) (add-after 'install 'wrap-program (lambda* (#:key outputs #:allow-other-keys) ;; Make sure everything can run in a pure environment. (let ((out (assoc-ref outputs "out")) (path (getenv "PERL5LIB"))) (for-each (lambda (file) (wrap-program file `("PERL5LIB" ":" prefix (,path))) (wrap-program file `("PATH" ":" prefix (,(string-append out "/bin") ,(dirname (which "sed")) ,(dirname (which "gzip")) ,(dirname (which "grep")) ,(dirname (which "perl")) ,(dirname (which "uname")))))) (find-files out "."))) #t)) (add-after 'wrap-program 'check (lambda* (#:key outputs #:allow-other-keys) (invoke (string-append (assoc-ref outputs "out") "/bin/edirect.pl") "-filter" "-help") #t))))) (inputs `(("edirect-go-programs" ,edirect-go-programs) ("perl-html-parser" ,perl-html-parser) ("perl-encode-locale" ,perl-encode-locale) ("perl-file-listing" ,perl-file-listing) ("perl-html-tagset" ,perl-html-tagset) ("perl-html-tree" ,perl-html-tree) ("perl-http-cookies" ,perl-http-cookies) ("perl-http-date" ,perl-http-date) ("perl-http-message" ,perl-http-message) ("perl-http-negotiate" ,perl-http-negotiate) ("perl-lwp-mediatypes" ,perl-lwp-mediatypes) ("perl-lwp-protocol-https" ,perl-lwp-protocol-https) ("perl-net-http" ,perl-net-http) ("perl-uri" ,perl-uri) ("perl-www-robotrules" ,perl-www-robotrules) ("perl-xml-simple" ,perl-xml-simple) ("perl" ,perl))) (home-page "https://www.ncbi.nlm.nih.gov/books/NBK179288/") (synopsis "Tools for accessing the NCBI's set of databases") (description "Entrez Direct (EDirect) is a method for accessing the National Center for Biotechnology Information's (NCBI) set of interconnected databases (publication, sequence, structure, gene, variation, expression, etc.) from a terminal. Functions take search terms from command-line arguments. Individual operations are combined to build multi-step queries. Record retrieval and formatting normally complete the process. EDirect also provides an argument-driven function that simplifies the extraction of data from document summaries or other results that are returned in structured XML format. This can eliminate the need for writing custom software to answer ad hoc questions.") (native-search-paths ;; Ideally this should be set for LWP somewhere. (list (search-path-specification (variable "PERL_LWP_SSL_CA_FILE") (file-type 'regular) (separator #f) (files '("/etc/ssl/certs/ca-certificates.crt"))))) (license license:public-domain))) (define-public edirect-go-programs (package (inherit edirect) (name "edirect-go-programs") (build-system go-build-system) (arguments `(#:install-source? #f #:tests? #f ; No tests. #:import-path "ncbi.nlm.nih.gov/entrez/edirect" #:phases (modify-phases %standard-phases (replace 'build (lambda* (#:key import-path #:allow-other-keys) (with-directory-excursion (string-append "src/" import-path) (invoke "go" "build" "-v" "-x" "j2x.go") (invoke "go" "build" "-v" "-x" "t2x.go") (invoke "go" "build" "-v" "-x" "-o" "xtract.Linux" "xtract.go" "common.go") (invoke "go" "build" "-v" "-x" "-o" "rchive.Linux" "rchive.go" "common.go") (invoke "go" "build" "-v" "-x" "-o" "symbols.Linux" "s2p.go")))) (replace 'install (lambda* (#:key outputs import-path #:allow-other-keys) (let ((dest (string-append (assoc-ref outputs "out") "/bin")) (source (string-append "src/" import-path "/"))) (for-each (lambda (file) (format #t "installing ~a~%" file) (install-file (string-append source file) dest)) '("j2x" "t2x" "symbols.Linux" "xtract.Linux" "rchive.Linux")) #t)))))) (native-inputs '()) (propagated-inputs '()) (inputs `(("go-github-com-fatih-color" ,go-github-com-fatih-color) ("go-github-com-fogleman-gg" ,go-github-com-fogleman-gg) ("go-github-com-gedex-inflector" ,go-github-com-gedex-inflector) ("go-github-com-golang-freetype" ,go-github-com-golang-freetype) ("go-github-com-klauspost-cpuid" ,go-github-com-klauspost-cpuid) ("go-github-com-pbnjay-memory" ,go-github-com-pbnjay-memory) ("go-github-com-surgebase-porter2" ,go-github-com-surgebase-porter2) ("go-golang-org-rainycape-unidecode" ,go-golang-org-rainycape-unidecode) ("go-golang-org-x-image" ,go-golang-org-x-image) ("go-golang-org-x-text" ,go-golang-org-x-text))))) (define-public exonerate (package (name "exonerate") (version "2.4.0") (source (origin (method url-fetch) (uri (string-append "http://ftp.ebi.ac.uk/pub/software/vertebrategenomics/exonerate/" "exonerate-" version ".tar.gz")) (sha256 (base32 "0hj0m9xygiqsdxvbg79wq579kbrx1mdrabi2bzqz2zn9qwfjcjgq")))) (build-system gnu-build-system) (arguments `(#:parallel-build? #f)) ; Building in parallel fails on some machines. (native-inputs `(("pkg-config" ,pkg-config))) (inputs `(("glib" ,glib))) (home-page "https://www.ebi.ac.uk/about/vertebrate-genomics/software/exonerate") (synopsis "Generic tool for biological sequence alignment") (description "Exonerate is a generic tool for pairwise sequence comparison. It allows the alignment of sequences using a many alignment models, either exhaustive dynamic programming or a variety of heuristics.") (license license:gpl3))) (define-public express (package (name "express") (version "1.5.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/adarob/eXpress") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "18nb22n7x820fzjngf4qgyb3mspqkw7xyk7v7s5ps6wfrd8qwscb")))) (build-system cmake-build-system) (arguments `(#:tests? #f ;no "check" target #:phases (modify-phases %standard-phases (add-after 'unpack 'use-shared-boost-libs-and-set-bamtools-paths (lambda* (#:key inputs #:allow-other-keys) (substitute* "CMakeLists.txt" (("set\\(Boost_USE_STATIC_LIBS ON\\)") "set(Boost_USE_STATIC_LIBS OFF)") (("\\$\\{CMAKE_CURRENT_SOURCE_DIR\\}/bamtools/include") (string-append (assoc-ref inputs "bamtools") "/include/bamtools"))) (substitute* "src/CMakeLists.txt" (("\\$\\{CMAKE_CURRENT_SOURCE_DIR\\}/\\.\\./bamtools/lib") (string-append (assoc-ref inputs "bamtools") "/lib")) (("libprotobuf.a") "libprotobuf.so")) #t)) (add-after 'unpack 'remove-update-check (lambda _ (substitute* "src/main.cpp" (("#include \"update_check.h\"") "") (("check_version\\(PACKAGE_VERSION\\);") "")) #t))))) (inputs `(("boost" ,boost) ("bamtools" ,bamtools) ("protobuf" ,protobuf) ("zlib" ,zlib))) (home-page "http://bio.math.berkeley.edu/eXpress") (synopsis "Streaming quantification for high-throughput genomic sequencing") (description "eXpress is a streaming tool for quantifying the abundances of a set of target sequences from sampled subsequences. Example applications include transcript-level RNA-Seq quantification, allele-specific/haplotype expression analysis (from RNA-Seq), transcription factor binding quantification in ChIP-Seq, and analysis of metagenomic data.") (license license:artistic2.0))) (define-public express-beta-diversity (package (name "express-beta-diversity") (version "1.0.8") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/dparks1134/ExpressBetaDiversity") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0s0yzg5c21349rh7x4w9266jsvnp7j1hp9cf8sk32hz8nvrj745x")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (delete 'configure) (add-before 'build 'enter-source (lambda _ (chdir "source") #t)) (replace 'check (lambda _ (invoke "../bin/ExpressBetaDiversity" "-u") #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (install-file "../scripts/convertToEBD.py" bin) (install-file "../bin/ExpressBetaDiversity" bin) #t)))))) (inputs `(("python" ,python-2))) (home-page "https://github.com/dparks1134/ExpressBetaDiversity") (synopsis "Taxon- and phylogenetic-based beta diversity measures") (description "Express Beta Diversity (EBD) calculates ecological beta diversity (dissimilarity) measures between biological communities. EBD implements a variety of diversity measures including those that make use of phylogenetic similarity of community members.") (license license:gpl3+))) (define-public fasttree (package (name "fasttree") (version "2.1.10") (source (origin (method url-fetch) (uri (string-append "http://www.microbesonline.org/fasttree/FastTree-" version ".c")) (sha256 (base32 "0vcjdvy1j4m702vmak4svbfkrpcw63k7wymfksjp9a982zy8kjsl")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no "check" target #:phases (modify-phases %standard-phases (delete 'unpack) (delete 'configure) (replace 'build (lambda* (#:key source #:allow-other-keys) (invoke "gcc" "-O3" "-finline-functions" "-funroll-loops" "-Wall" "-o" "FastTree" source "-lm") (invoke "gcc" "-DOPENMP" "-fopenmp" "-O3" "-finline-functions" "-funroll-loops" "-Wall" "-o" "FastTreeMP" source "-lm") #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (install-file "FastTree" bin) (install-file "FastTreeMP" bin) #t)))))) (home-page "http://www.microbesonline.org/fasttree") (synopsis "Infers approximately-maximum-likelihood phylogenetic trees") (description "FastTree can handle alignments with up to a million of sequences in a reasonable amount of time and memory. For large alignments, FastTree is 100-1,000 times faster than PhyML 3.0 or RAxML 7.") (license license:gpl2+))) (define-public fastx-toolkit (package (name "fastx-toolkit") (version "0.0.14") (source (origin (method url-fetch) (uri (string-append "https://github.com/agordon/fastx_toolkit/releases/download/" version "/fastx_toolkit-" version ".tar.bz2")) (sha256 (base32 "01jqzw386873sr0pjp1wr4rn8fsga2vxs1qfmicvx1pjr72007wy")))) (build-system gnu-build-system) (inputs `(("libgtextutils" ,libgtextutils))) (native-inputs `(("gcc" ,gcc-6) ;; doesn't build with later versions ("pkg-config" ,pkg-config))) (home-page "http://hannonlab.cshl.edu/fastx_toolkit/") (synopsis "Tools for FASTA/FASTQ file preprocessing") (description "The FASTX-Toolkit is a collection of command line tools for Short-Reads FASTA/FASTQ files preprocessing. Next-Generation sequencing machines usually produce FASTA or FASTQ files, containing multiple short-reads sequences. The main processing of such FASTA/FASTQ files is mapping the sequences to reference genomes. However, it is sometimes more productive to preprocess the files before mapping the sequences to the genome---manipulating the sequences to produce better mapping results. The FASTX-Toolkit tools perform some of these preprocessing tasks.") (license license:agpl3+))) (define-public flexbar (package (name "flexbar") (version "3.4.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/seqan/flexbar") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1pq9sxvdnldl14libk234m72dqhwgzs3acgl943wchwdqlcsi5r2")))) (build-system cmake-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'do-not-tune-to-CPU (lambda _ (substitute* "src/CMakeLists.txt" ((" -march=native") "")) #t)) (replace 'check (lambda* (#:key outputs #:allow-other-keys) (setenv "PATH" (string-append (getcwd) ":" (getenv "PATH"))) (with-directory-excursion "../source/test" (invoke "bash" "flexbar_test.sh")) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (string-append (assoc-ref outputs "out"))) (bin (string-append out "/bin/"))) (install-file "flexbar" bin)) #t))))) (inputs `(("tbb" ,tbb) ("zlib" ,zlib))) (native-inputs `(("pkg-config" ,pkg-config) ("seqan" ,seqan))) (home-page "https://github.com/seqan/flexbar") (synopsis "Barcode and adapter removal tool for sequencing platforms") (description "Flexbar preprocesses high-throughput nucleotide sequencing data efficiently. It demultiplexes barcoded runs and removes adapter sequences. Moreover, trimming and filtering features are provided. Flexbar increases read mapping rates and improves genome and transcriptome assemblies. It supports next-generation sequencing data in fasta/q and csfasta/q format from Illumina, Roche 454, and the SOLiD platform.") (license license:bsd-3))) (define-public fraggenescan (package (name "fraggenescan") (version "1.30") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/fraggenescan/" "FragGeneScan" version ".tar.gz")) (sha256 (base32 "158dcnwczgcyhwm4qlx19sanrwgdpzf6bn2y57mbpx55lkgz1mzj")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (delete 'configure) (add-before 'build 'patch-paths (lambda* (#:key outputs #:allow-other-keys) (let* ((out (string-append (assoc-ref outputs "out"))) (share (string-append out "/share/fraggenescan/"))) (substitute* "run_FragGeneScan.pl" (("system\\(\"rm") (string-append "system(\"" (which "rm"))) (("system\\(\"mv") (string-append "system(\"" (which "mv"))) (("\\\"awk") (string-append "\"" (which "awk"))) ;; This script and other programs expect the training files ;; to be in the non-standard location bin/train/XXX. Change ;; this to be share/fraggenescan/train/XXX instead. (("^\\$train.file = \\$dir.*") (string-append "$train_file = \"" share "train/\".$FGS_train_file;"))) (substitute* "run_hmm.c" (("^ strcat\\(train_dir, \\\"train/\\\"\\);") (string-append " strcpy(train_dir, \"" share "/train/\");")))) #t)) (replace 'build (lambda _ (invoke "make" "clean") (invoke "make" "fgs") #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (string-append (assoc-ref outputs "out"))) (bin (string-append out "/bin/")) (share (string-append out "/share/fraggenescan/train"))) (install-file "run_FragGeneScan.pl" bin) (install-file "FragGeneScan" bin) (copy-recursively "train" share)) #t)) (delete 'check) (add-after 'install 'post-install-check ;; In lieu of 'make check', run one of the examples and check the ;; output files gets created. (lambda* (#:key outputs #:allow-other-keys) (let* ((out (string-append (assoc-ref outputs "out"))) (bin (string-append out "/bin/")) (frag (string-append bin "run_FragGeneScan.pl"))) ;; Test complete genome. (invoke frag "-genome=./example/NC_000913.fna" "-out=./test2" "-complete=1" "-train=complete") (unless (and (file-exists? "test2.faa") (file-exists? "test2.ffn") (file-exists? "test2.gff") (file-exists? "test2.out")) (error "Expected files do not exist.")) ;; Test incomplete sequences. (invoke frag "-genome=./example/NC_000913-fgs.ffn" "-out=out" "-complete=0" "-train=454_30") #t)))))) (inputs `(("perl" ,perl) ("python" ,python-2))) ;not compatible with python 3. (home-page "https://sourceforge.net/projects/fraggenescan/") (synopsis "Finds potentially fragmented genes in short reads") (description "FragGeneScan is a program for predicting bacterial and archaeal genes in short and error-prone DNA sequencing reads. It can also be applied to predict genes in incomplete assemblies or complete genomes.") ;; GPL3+ according to private correspondense with the authors. (license license:gpl3+))) (define-public fxtract (let ((util-commit "776ca85a18a47492af3794745efcb4a905113115")) (package (name "fxtract") (version "2.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ctSkennerton/fxtract") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "0hab3gpwf4w9s87qlbswq6ws1qqybh4dcqk79q1ahyldzai5fgp5")))) (build-system gnu-build-system) (arguments `(#:make-flags (list (string-append "PREFIX=" (assoc-ref %outputs "out")) "CC=gcc") #:test-target "fxtract_test" #:phases (modify-phases %standard-phases (delete 'configure) (add-before 'build 'copy-util (lambda* (#:key inputs #:allow-other-keys) (rmdir "util") (copy-recursively (assoc-ref inputs "ctskennerton-util") "util") #t)) ;; Do not use make install as this requires additional dependencies. (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out"/bin"))) (install-file "fxtract" bin) #t)))))) (inputs `(("pcre" ,pcre) ("zlib" ,zlib))) (native-inputs ;; ctskennerton-util is licensed under GPL2. `(("ctskennerton-util" ,(origin (method git-fetch) (uri (git-reference (url "https://github.com/ctSkennerton/util") (commit util-commit))) (file-name (string-append "ctstennerton-util-" util-commit "-checkout")) (sha256 (base32 "0cls1hd4vgj3f36fpzzg4xc77d6f3hpc60cbpfmn2gdr7ykzzad7")))))) (home-page "https://github.com/ctSkennerton/fxtract") (synopsis "Extract sequences from FASTA and FASTQ files") (description "Fxtract extracts sequences from a protein or nucleotide fastx (FASTA or FASTQ) file given a subsequence. It uses a simple substring search for basic tasks but can change to using POSIX regular expressions, PCRE, hash lookups or multi-pattern searching as required. By default fxtract looks in the sequence of each record but can also be told to look in the header, comment or quality sections.") ;; 'util' requires SSE instructions. (supported-systems '("x86_64-linux")) (license license:expat)))) (define-public gemma (package (name "gemma") (version "0.98.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/genetics-statistics/GEMMA") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1p8a7kkfn1mmrg017aziy544aha8i9h6wd1x2dk3w2794wl33qb7")) (modules '((guix build utils))) (snippet '(begin (delete-file-recursively "contrib") #t)))) (build-system gnu-build-system) (inputs `(("gsl" ,gsl) ("openblas" ,openblas) ("zlib" ,zlib))) (native-inputs `(("catch" ,catch-framework2-1) ("perl" ,perl) ("shunit2" ,shunit2) ("which" ,which))) (arguments `(#:phases (modify-phases %standard-phases (delete 'configure) (add-after 'unpack 'prepare-build (lambda* (#:key inputs #:allow-other-keys) (mkdir-p "bin") (substitute* "Makefile" (("/usr/local/opt/openblas") (assoc-ref inputs "openblas"))) #t)) (replace 'check (lambda* (#:key tests? #:allow-other-keys) (when tests? ;; 'make slow-check' expects shunit2-2.0.3. (with-directory-excursion "test" (invoke "./test_suite.sh")) #t))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (install-file "bin/gemma" (string-append (assoc-ref outputs "out") "/bin")) #t))))) (home-page "https://github.com/genetics-statistics/GEMMA") (synopsis "Tool for genome-wide efficient mixed model association") (description "@acronym{GEMMA, Genome-wide Efficient Mixed Model Association} provides a standard linear mixed model resolver with application in @acronym{GWAS, genome-wide association studies}.") (license license:gpl3))) (define-public grit (package (name "grit") (version "2.0.5") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/nboley/grit") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1l5v8vfvfbrpmgnrvbrbv40d0arhxcnmxgv2f1mlcqfa3q6bkqm9")))) (build-system python-build-system) (arguments `(#:python ,python-2 #:phases (modify-phases %standard-phases (add-after 'unpack 'generate-from-cython-sources (lambda* (#:key inputs outputs #:allow-other-keys) ;; Delete these C files to force fresh generation from pyx sources. (delete-file "grit/sparsify_support_fns.c") (delete-file "grit/call_peaks_support_fns.c") (substitute* "setup.py" (("Cython.Setup") "Cython.Build")) #t))))) (inputs `(("python-scipy" ,python2-scipy) ("python-numpy" ,python2-numpy) ("python-pysam" ,python2-pysam) ("python-networkx" ,python2-networkx))) (native-inputs `(("python-cython" ,python2-cython))) ;; The canonical home page times out as of 2020-01-21. (home-page "https://github.com/nboley/grit") (synopsis "Tool for integrative analysis of RNA-seq type assays") (description "GRIT is designed to use RNA-seq, TES, and TSS data to build and quantify full length transcript models. When none of these data sources are available, GRIT can be run by providing a candidate set of TES or TSS sites. In addition, GRIT can merge in reference junctions and gene boundaries. GRIT can also be run in quantification mode, where it uses a provided GTF file and just estimates transcript expression.") (license license:gpl3+))) (define-public hisat (package (name "hisat") (version "0.1.4") (source (origin (method url-fetch) (uri (string-append "http://ccb.jhu.edu/software/hisat/downloads/hisat-" version "-beta-source.zip")) (sha256 (base32 "1k381ydranqxp09yf2y7w1d0chz5d59vb6jchi89hbb0prq19lk5")))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no check target #:make-flags '("allall" ;; Disable unsupported `popcnt' instructions on ;; architectures other than x86_64 ,@(if (string-prefix? "x86_64" (or (%current-target-system) (%current-system))) '() '("POPCNT_CAPABILITY=0"))) #:phases (modify-phases %standard-phases (add-after 'unpack 'patch-sources (lambda _ ;; XXX Cannot use snippet because zip files are not supported (substitute* "Makefile" (("^CC = .*$") "CC = gcc") (("^CPP = .*$") "CPP = g++") ;; replace BUILD_HOST and BUILD_TIME for deterministic build (("-DBUILD_HOST=.*") "-DBUILD_HOST=\"\\\"guix\\\"\"") (("-DBUILD_TIME=.*") "-DBUILD_TIME=\"\\\"0\\\"\"")) (substitute* '("hisat-build" "hisat-inspect") (("/usr/bin/env") (which "env"))) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (for-each (lambda (file) (install-file file bin)) (find-files "." "hisat(-(build|align|inspect)(-(s|l)(-debug)*)*)*$"))) #t)) (delete 'configure)))) (native-inputs `(("unzip" ,unzip))) (inputs `(("perl" ,perl) ("python" ,python) ("zlib" ,zlib))) ;; Non-portable SSE instructions are used so building fails on platforms ;; other than x86_64. (supported-systems '("x86_64-linux")) (home-page "https://ccb.jhu.edu/software/hisat/index.shtml") (synopsis "Hierarchical indexing for spliced alignment of transcripts") (description "HISAT is a fast and sensitive spliced alignment program for mapping RNA-seq reads. In addition to one global FM index that represents a whole genome, HISAT uses a large set of small FM indexes that collectively cover the whole genome. These small indexes (called local indexes) combined with several alignment strategies enable effective alignment of RNA-seq reads, in particular, reads spanning multiple exons.") (license license:gpl3+))) (define-public hisat2 (package (name "hisat2") (version "2.2.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/DaehwanKimLab/hisat2/") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0lmzdhzjkvxw7n5w40pbv5fgzd4cz0f9pxczswn3d4cr0k10k754")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no check target #:make-flags (list "CC=gcc" "CXX=g++" "allall") #:modules ((guix build gnu-build-system) (guix build utils) (srfi srfi-26)) #:phases (modify-phases %standard-phases (add-after 'unpack 'make-deterministic (lambda _ (substitute* "Makefile" (("`date`") "0")))) (delete 'configure) (add-before 'build 'build-manual (lambda _ (mkdir-p "doc") (invoke "make" "doc"))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin/")) (doc (string-append out "/share/doc/hisat2/"))) (for-each (cut install-file <> bin) (find-files "." "hisat2(-(build|align|inspect)(-(s|l)(-debug)*)*)*$")) (mkdir-p doc) (install-file "doc/manual.inc.html" doc))))))) (native-inputs `(("perl" ,perl) ("pandoc" ,pandoc))) ; for documentation (inputs `(("python" ,python-wrapper))) (home-page "https://daehwankimlab.github.io/hisat2/") (synopsis "Graph-based alignment of genomic sequencing reads") (description "HISAT2 is a fast and sensitive alignment program for mapping next-generation sequencing reads (both DNA and RNA) to a population of human genomes (as well as to a single reference genome). In addition to using one global @dfn{graph FM} (GFM) index that represents a population of human genomes, HISAT2 uses a large set of small GFM indexes that collectively cover the whole genome. These small indexes, combined with several alignment strategies, enable rapid and accurate alignment of sequencing reads. This new indexing scheme is called a @dfn{Hierarchical Graph FM index} (HGFM).") ;; HISAT2 contains files from Bowtie2, which is released under ;; GPLv2 or later. The HISAT2 source files are released under ;; GPLv3 or later. (license license:gpl3+))) (define-public hmmer (package (name "hmmer") (version "3.3.2") (source (origin (method url-fetch) (uri (string-append "http://eddylab.org/software/hmmer/hmmer-" version ".tar.gz")) (sha256 (base32 "0s9wf6n0qanbx8qs6igfl3vyjikwbrvh4d9d6mv54yp3xysykzlj")))) (build-system gnu-build-system) (native-inputs `(("perl" ,perl) ("python" ,python))) ; for tests (home-page "http://hmmer.org/") (synopsis "Biosequence analysis using profile hidden Markov models") (description "HMMER is used for searching sequence databases for homologs of protein sequences, and for making protein sequence alignments. It implements methods using probabilistic models called profile hidden Markov models (profile HMMs).") ;; hmmer uses non-portable SSE intrinsics so building fails on other ;; platforms. (supported-systems '("x86_64-linux" "i686-linux")) (license license:bsd-3))) (define-public htseq (package (name "htseq") (version "0.9.1") (source (origin (method url-fetch) (uri (pypi-uri "HTSeq" version)) (sha256 (base32 "11flgb1381xdhk43bzbfm3vhnszkpqg6jk76rpa5xd1zbrvvlnxg")))) (build-system python-build-system) (native-inputs `(("python-cython" ,python-cython))) ;; Numpy needs to be propagated when htseq is used as a Python library. (propagated-inputs `(("python-numpy" ,python-numpy))) (inputs `(("python-pysam" ,python-pysam) ("python-matplotlib" ,python-matplotlib))) (home-page "https://htseq.readthedocs.io/") (synopsis "Analysing high-throughput sequencing data with Python") (description "HTSeq is a Python package that provides infrastructure to process data from high-throughput sequencing assays.") (license license:gpl3+))) (define-public python2-htseq (package-with-python2 htseq)) (define-public java-htsjdk (package (name "java-htsjdk") (version "2.3.0") ; last version without build dependency on gradle (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/samtools/htsjdk") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1b178ixcabanm834ydjl3jiakpyxdmki32hqfv2abrzn3rcwa28i")) (modules '((guix build utils))) (snippet ;; Delete pre-built binaries '(begin (delete-file-recursively "lib") (mkdir-p "lib") #t)))) (build-system ant-build-system) (arguments `(#:tests? #f ; test require Internet access #:jdk ,icedtea-8 #:make-flags (list (string-append "-Ddist=" (assoc-ref %outputs "out") "/share/java/htsjdk/")) #:build-target "all" #:phases (modify-phases %standard-phases ;; The build phase also installs the jars (delete 'install)))) (inputs `(("java-ngs" ,java-ngs) ("java-snappy-1" ,java-snappy-1) ("java-commons-compress" ,java-commons-compress) ("java-commons-logging-minimal" ,java-commons-logging-minimal) ("java-commons-jexl-2" ,java-commons-jexl-2) ("java-xz" ,java-xz))) (native-inputs `(("java-testng" ,java-testng))) (home-page "http://samtools.github.io/htsjdk/") (synopsis "Java API for high-throughput sequencing data (HTS) formats") (description "HTSJDK is an implementation of a unified Java library for accessing common file formats, such as SAM and VCF, used for high-throughput sequencing (HTS) data. There are also an number of useful utilities for manipulating HTS data.") (license license:expat))) (define-public java-htsjdk-latest (package (name "java-htsjdk") (version "2.14.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/samtools/htsjdk") (commit version))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "1lmya1fdjy03mz6zmdmd86j9v9vfhqb3952mqq075navx1i6g4bc")))) (build-system ant-build-system) (arguments `(#:tests? #f ; test require Scala #:jdk ,icedtea-8 #:jar-name "htsjdk.jar" #:phases (modify-phases %standard-phases (add-after 'unpack 'remove-useless-build.xml (lambda _ (delete-file "build.xml") #t)) ;; The tests require the scalatest package. (add-after 'unpack 'remove-tests (lambda _ (delete-file-recursively "src/test") #t))))) (inputs `(("java-ngs" ,java-ngs) ("java-snappy-1" ,java-snappy-1) ("java-commons-compress" ,java-commons-compress) ("java-commons-logging-minimal" ,java-commons-logging-minimal) ("java-commons-jexl-2" ,java-commons-jexl-2) ("java-xz" ,java-xz))) (native-inputs `(("java-junit" ,java-junit))) (home-page "http://samtools.github.io/htsjdk/") (synopsis "Java API for high-throughput sequencing data (HTS) formats") (description "HTSJDK is an implementation of a unified Java library for accessing common file formats, such as SAM and VCF, used for high-throughput sequencing (HTS) data. There are also an number of useful utilities for manipulating HTS data.") (license license:expat))) ;; This is needed for picard 2.10.3 (define-public java-htsjdk-2.10.1 (package (inherit java-htsjdk-latest) (name "java-htsjdk") (version "2.10.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/samtools/htsjdk") (commit version))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "1kxh7slm2pm3x9p6jxa1wqsq9a31dhiiflhxnxqcisan4k3rwia2")))) (build-system ant-build-system) (arguments `(#:tests? #f ; tests require Scala #:jdk ,icedtea-8 #:jar-name "htsjdk.jar" #:phases (modify-phases %standard-phases (add-after 'unpack 'remove-useless-build.xml (lambda _ (delete-file "build.xml") #t)) ;; The tests require the scalatest package. (add-after 'unpack 'remove-tests (lambda _ (delete-file-recursively "src/test") #t))))))) ;; This version matches java-htsjdk 2.3.0. Later versions also require a more ;; recent version of java-htsjdk, which depends on gradle. (define-public java-picard (package (name "java-picard") (version "2.3.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/broadinstitute/picard") (commit version))) (file-name (string-append "java-picard-" version "-checkout")) (sha256 (base32 "1ll7mf4r3by92w2nhlmpa591xd1f46xlkwh59mq6fvbb5pdwzvx6")) (modules '((guix build utils))) (snippet '(begin ;; Delete pre-built binaries. (delete-file-recursively "lib") (mkdir-p "lib") (substitute* "build.xml" ;; Remove build-time dependency on git. (("failifexecutionfails=\"true\"") "failifexecutionfails=\"false\"") ;; Use our htsjdk. (("depends=\"compile-htsjdk, ") "depends=\"") (("depends=\"compile-htsjdk-tests, ") "depends=\"") ;; Build picard-lib.jar before building picard.jar (("name=\"picard-jar\" depends=\"" line) (string-append line "picard-lib-jar, "))) #t)))) (build-system ant-build-system) (arguments `(#:build-target "picard-jar" #:test-target "test" ;; Tests require jacoco:coverage. #:tests? #f #:make-flags (list (string-append "-Dhtsjdk_lib_dir=" (assoc-ref %build-inputs "java-htsjdk") "/share/java/htsjdk/") "-Dhtsjdk-classes=dist/tmp" (string-append "-Dhtsjdk-version=" ,(package-version java-htsjdk))) #:jdk ,icedtea-8 #:phases (modify-phases %standard-phases ;; FIXME: this phase fails with "duplicate entry: htsjdk/samtools/AbstractBAMFileIndex$1.class" (delete 'generate-jar-indices) (add-after 'unpack 'use-our-htsjdk (lambda* (#:key inputs #:allow-other-keys) (substitute* "build.xml" (("\\$\\{htsjdk\\}/lib") (string-append (assoc-ref inputs "java-htsjdk") "/share/java/htsjdk/"))) #t)) (add-after 'unpack 'make-test-target-independent (lambda* (#:key inputs #:allow-other-keys) (substitute* "build.xml" (("name=\"test\" depends=\"compile, ") "name=\"test\" depends=\"")) #t)) (replace 'install (install-jars "dist"))))) (inputs `(("java-htsjdk" ,java-htsjdk) ("java-guava" ,java-guava))) (native-inputs `(("java-testng" ,java-testng))) (home-page "http://broadinstitute.github.io/picard/") (synopsis "Tools for manipulating high-throughput sequencing data and formats") (description "Picard is a set of Java command line tools for manipulating high-throughput sequencing (HTS) data and formats. Picard is implemented using the HTSJDK Java library to support accessing file formats that are commonly used for high-throughput sequencing data such as SAM, BAM, CRAM and VCF.") (license license:expat))) ;; This is needed for dropseq-tools (define-public java-picard-2.10.3 (package (name "java-picard") (version "2.10.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/broadinstitute/picard") (commit version))) (file-name (string-append "java-picard-" version "-checkout")) (sha256 (base32 "1ajlx31l6i1k3y2rhnmgq07sz99g2czqfqgkr9mihmdjp3gwjhvi")))) (build-system ant-build-system) (arguments `(#:jar-name "picard.jar" ;; Tests require jacoco:coverage. #:tests? #f #:jdk ,icedtea-8 #:main-class "picard.cmdline.PicardCommandLine" #:modules ((guix build ant-build-system) (guix build utils) (guix build java-utils) (sxml simple) (sxml transform) (sxml xpath)) #:phases (modify-phases %standard-phases ;; FIXME: this phase fails with "duplicate entry: htsjdk/samtools/AbstractBAMFileIndex$1.class" (delete 'generate-jar-indices) (add-after 'unpack 'remove-useless-build.xml (lambda _ (delete-file "build.xml") #t)) ;; This is necessary to ensure that htsjdk is found when using ;; picard.jar as an executable. (add-before 'build 'edit-classpath-in-manifest (lambda* (#:key inputs #:allow-other-keys) (chmod "build.xml" #o664) (call-with-output-file "build.xml.new" (lambda (port) (sxml->xml (pre-post-order (with-input-from-file "build.xml" (lambda _ (xml->sxml #:trim-whitespace? #t))) `((target . ,(lambda (tag . kids) (let ((name ((sxpath '(name *text*)) (car kids))) ;; FIXME: We're breaking the line ;; early with a dummy path to ;; ensure that the store reference ;; isn't broken apart and can still ;; be found by the reference ;; scanner. (msg (format #f "\ Class-Path: /~a \ ~a/share/java/htsjdk.jar${line.separator}${line.separator}" ;; maximum line length is 70 (string-tabulate (const #\b) 57) (assoc-ref inputs "java-htsjdk")))) (if (member "manifest" name) `(,tag ,@kids (replaceregexp (@ (file "${manifest.file}") (match "\\r\\n\\r\\n") (replace "${line.separator}"))) (echo (@ (message ,msg) (file "${manifest.file}") (append "true")))) `(,tag ,@kids))))) (*default* . ,(lambda (tag . kids) `(,tag ,@kids))) (*text* . ,(lambda (_ txt) txt)))) port))) (rename-file "build.xml.new" "build.xml") #t))))) (propagated-inputs `(("java-htsjdk" ,java-htsjdk-2.10.1))) (native-inputs `(("java-testng" ,java-testng) ("java-guava" ,java-guava))) (home-page "http://broadinstitute.github.io/picard/") (synopsis "Tools for manipulating high-throughput sequencing data and formats") (description "Picard is a set of Java command line tools for manipulating high-throughput sequencing (HTS) data and formats. Picard is implemented using the HTSJDK Java library to support accessing file formats that are commonly used for high-throughput sequencing data such as SAM, BAM, CRAM and VCF.") (license license:expat))) ;; This is the last version of Picard to provide net.sf.samtools (define-public java-picard-1.113 (package (inherit java-picard) (name "java-picard") (version "1.113") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/broadinstitute/picard") (commit version))) (file-name (string-append "java-picard-" version "-checkout")) (sha256 (base32 "0lkpvin2fz3hhly4l02kk56fqy8lmlgyzr9kmvljk6ry6l1hw973")) (modules '((guix build utils))) (snippet '(begin ;; Delete pre-built binaries. (delete-file-recursively "lib") (mkdir-p "lib") #t)))) (build-system ant-build-system) (arguments `(#:build-target "picard-jar" #:test-target "test" ;; FIXME: the class path at test time is wrong. ;; [testng] Error: A JNI error has occurred, please check your installation and try again ;; [testng] Exception in thread "main" java.lang.NoClassDefFoundError: com/beust/jcommander/ParameterException #:tests? #f #:jdk ,icedtea-8 #:ant ,ant/java8 ;; This is only used for tests. #:make-flags (list "-Dsamjdk.intel_deflater_so_path=lib/jni/libIntelDeflater.so") #:phases (modify-phases %standard-phases ;; FIXME: This phase fails. (delete 'generate-jar-indices) ;; Do not use bundled ant bzip2. (add-after 'unpack 'use-ant-bzip (lambda* (#:key inputs #:allow-other-keys) (substitute* "build.xml" (("\\$\\{lib\\}/apache-ant-1.8.2-bzip2.jar") (string-append (assoc-ref inputs "ant") "/lib/ant.jar"))) #t)) (add-after 'unpack 'make-test-target-independent (lambda* (#:key inputs #:allow-other-keys) (substitute* "build.xml" (("name=\"test\" depends=\"compile, ") "name=\"test\" depends=\"compile-tests, ") (("name=\"compile\" depends=\"compile-src, compile-tests\"") "name=\"compile\" depends=\"compile-src\"")) #t)) (add-after 'unpack 'fix-deflater-path (lambda* (#:key outputs #:allow-other-keys) (substitute* "src/java/net/sf/samtools/Defaults.java" (("getStringProperty\\(\"intel_deflater_so_path\", null\\)") (string-append "getStringProperty(\"intel_deflater_so_path\", \"" (assoc-ref outputs "out") "/lib/jni/libIntelDeflater.so" "\")"))) #t)) ;; Build the deflater library, because we've previously deleted the ;; pre-built one. This can only be built with access to the JDK ;; sources. (add-after 'build 'build-jni (lambda* (#:key inputs #:allow-other-keys) (mkdir-p "lib/jni") (mkdir-p "jdk-src") (invoke "tar" "--strip-components=1" "-C" "jdk-src" "-xf" (assoc-ref inputs "jdk-src")) (invoke "javah" "-jni" "-classpath" "classes" "-d" "lib/" "net.sf.samtools.util.zip.IntelDeflater") (with-directory-excursion "src/c/inteldeflater" (invoke "gcc" "-I../../../lib" "-I." (string-append "-I" (assoc-ref inputs "jdk") "/include/linux") "-I../../../jdk-src/src/share/native/common/" "-I../../../jdk-src/src/solaris/native/common/" "-c" "-O3" "-fPIC" "IntelDeflater.c") (invoke "gcc" "-shared" "-o" "../../../lib/jni/libIntelDeflater.so" "IntelDeflater.o" "-lz" "-lstdc++")) #t)) ;; We can only build everything else after building the JNI library. (add-after 'build-jni 'build-rest (lambda* (#:key make-flags #:allow-other-keys) (apply invoke `("ant" "all" ,@make-flags)) #t)) (add-before 'build 'set-JAVA6_HOME (lambda _ (setenv "JAVA6_HOME" (getenv "JAVA_HOME")) #t)) (replace 'install (install-jars "dist")) (add-after 'install 'install-jni-lib (lambda* (#:key outputs #:allow-other-keys) (let ((jni (string-append (assoc-ref outputs "out") "/lib/jni"))) (mkdir-p jni) (install-file "lib/jni/libIntelDeflater.so" jni) #t)))))) (inputs `(("java-snappy-1" ,java-snappy-1) ("java-commons-jexl-2" ,java-commons-jexl-2) ("java-cofoja" ,java-cofoja) ("ant" ,ant/java8) ; for bzip2 support at runtime ("zlib" ,zlib))) (native-inputs `(("ant-apache-bcel" ,ant-apache-bcel) ("ant-junit" ,ant-junit) ("java-testng" ,java-testng) ("java-commons-bcel" ,java-commons-bcel) ("java-jcommander" ,java-jcommander) ("jdk" ,icedtea-8 "jdk") ("jdk-src" ,(car (assoc-ref (package-native-inputs icedtea-8) "jdk-drop"))))))) (define-public fastqc (package (name "fastqc") (version "0.11.5") (source (origin (method url-fetch) (uri (string-append "http://www.bioinformatics.babraham.ac.uk/" "projects/fastqc/fastqc_v" version "_source.zip")) (sha256 (base32 "18rrlkhcrxvvvlapch4dpj6xc6mpayzys8qfppybi8jrpgx5cc5f")))) (build-system ant-build-system) (arguments `(#:tests? #f ; there are no tests #:build-target "build" #:phases (modify-phases %standard-phases (add-after 'unpack 'fix-dependencies (lambda* (#:key inputs #:allow-other-keys) (substitute* "build.xml" (("jbzip2-0.9.jar") (string-append (assoc-ref inputs "java-jbzip2") "/share/java/jbzip2.jar")) (("sam-1.103.jar") (string-append (assoc-ref inputs "java-picard-1.113") "/share/java/sam-1.112.jar")) (("cisd-jhdf5.jar") (string-append (assoc-ref inputs "java-cisd-jhdf5") "/share/java/sis-jhdf5.jar"))) #t)) ;; There is no installation target (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (share (string-append out "/share/fastqc/")) (exe (string-append share "/fastqc"))) (for-each mkdir-p (list bin share)) (copy-recursively "bin" share) (substitute* exe (("my \\$java_bin = 'java';") (string-append "my $java_bin = '" (assoc-ref inputs "java") "/bin/java';"))) (chmod exe #o555) (symlink exe (string-append bin "/fastqc")) #t)))))) (inputs `(("java" ,icedtea) ("perl" ,perl) ; needed for the wrapper script ("java-cisd-jhdf5" ,java-cisd-jhdf5) ("java-picard-1.113" ,java-picard-1.113) ("java-jbzip2" ,java-jbzip2))) (native-inputs `(("unzip" ,unzip))) (home-page "https://www.bioinformatics.babraham.ac.uk/projects/fastqc/") (synopsis "Quality control tool for high throughput sequence data") (description "FastQC aims to provide a simple way to do some quality control checks on raw sequence data coming from high throughput sequencing pipelines. It provides a modular set of analyses which you can use to give a quick impression of whether your data has any problems of which you should be aware before doing any further analysis. The main functions of FastQC are: @itemize @item Import of data from BAM, SAM or FastQ files (any variant); @item Providing a quick overview to tell you in which areas there may be problems; @item Summary graphs and tables to quickly assess your data; @item Export of results to an HTML based permanent report; @item Offline operation to allow automated generation of reports without running the interactive application. @end itemize\n") (license license:gpl3+))) (define-public fastp (package (name "fastp") (version "0.14.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/OpenGene/fastp") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1r6ms5zbf5rps4rgp4z73nczadl00b5rqylw8f684isfz27dp0xh")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are none #:make-flags (list (string-append "BINDIR=" (assoc-ref %outputs "out") "/bin")) #:phases (modify-phases %standard-phases (delete 'configure) (add-before 'install 'create-target-dir (lambda* (#:key outputs #:allow-other-keys) (mkdir-p (string-append (assoc-ref outputs "out") "/bin")) #t))))) (inputs `(("zlib" ,zlib))) (home-page "https://github.com/OpenGene/fastp/") (synopsis "All-in-one FastQ preprocessor") (description "Fastp is a tool designed to provide fast all-in-one preprocessing for FastQ files. This tool has multi-threading support to afford high performance.") (license license:expat))) (define-public htslib (package (name "htslib") (version "1.12") (source (origin (method url-fetch) (uri (string-append "https://github.com/samtools/htslib/releases/download/" version "/htslib-" version ".tar.bz2")) (sha256 (base32 "1jplnvizgr0fyyvvmkfmnsywrrpqhid3760vw15bllz98qdi9012")))) (build-system gnu-build-system) ;; Let htslib translate "gs://" and "s3://" to regular https links with ;; "--enable-gcs" and "--enable-s3". For these options to work, we also ;; need to set "--enable-libcurl". (arguments `(#:configure-flags '("--enable-gcs" "--enable-libcurl" "--enable-s3"))) (inputs `(("curl" ,curl) ("openssl" ,openssl))) ;; This is referred to in the pkg-config file as a required library. (propagated-inputs `(("zlib" ,zlib))) (native-inputs `(("perl" ,perl))) (home-page "https://www.htslib.org") (synopsis "C library for reading/writing high-throughput sequencing data") (description "HTSlib is a C library for reading/writing high-throughput sequencing data. It also provides the @command{bgzip}, @command{htsfile}, and @command{tabix} utilities.") ;; Files under cram/ are released under the modified BSD license; ;; the rest is released under the Expat license (license (list license:expat license:bsd-3)))) (define-public htslib-1.9 (package (inherit htslib) (name "htslib") (version "1.9") (source (origin (method url-fetch) (uri (string-append "https://github.com/samtools/htslib/releases/download/" version "/htslib-" version ".tar.bz2")) (sha256 (base32 "16ljv43sc3fxmv63w7b2ff8m1s7h89xhazwmbm1bicz8axq8fjz0")))))) ;; This package should be removed once no packages rely upon it. (define htslib-1.3 (package (inherit htslib) (version "1.3.1") (source (origin (method url-fetch) (uri (string-append "https://github.com/samtools/htslib/releases/download/" version "/htslib-" version ".tar.bz2")) (sha256 (base32 "1rja282fwdc25ql6izkhdyh8ppw8x2fs0w0js78zgkmqjlikmma9")))))) (define-public idr (package (name "idr") (version "2.0.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/nboley/idr") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "04j876h6z444v2q79drxx283d3k5snd72kj895wbalnl42206x9g")) ;; Delete generated C code. (snippet '(begin (delete-file "idr/inv_cdf.c") #t)))) (build-system python-build-system) ;; There is only one test ("test_inv_cdf.py") and it tests features that ;; are no longer part of this package. It also asserts False, which ;; causes the tests to always fail. (arguments `(#:tests? #f)) (propagated-inputs `(("python-scipy" ,python-scipy) ("python-sympy" ,python-sympy) ("python-numpy" ,python-numpy) ("python-matplotlib" ,python-matplotlib))) (native-inputs `(("python-cython" ,python-cython))) (home-page "https://github.com/nboley/idr") (synopsis "Tool to measure the irreproducible discovery rate (IDR)") (description "The IDR (Irreproducible Discovery Rate) framework is a unified approach to measure the reproducibility of findings identified from replicate experiments and provide highly stable thresholds based on reproducibility.") (license license:gpl2+))) (define-public jellyfish (package (name "jellyfish") (version "2.3.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/gmarcais/Jellyfish/" "releases/download/v" version "/jellyfish-" version ".tar.gz")) (sha256 (base32 "0npa62wzasdibas5zp3n8j3armsci4kyvh0jw7jr0am4gg7vg5g1")))) (build-system gnu-build-system) (outputs '("out" ;for library "ruby" ;for Ruby bindings "python")) ;for Python bindings (arguments `(#:configure-flags (list "--without-sse" ; configure script probes for CPU features when SSE is enabled. (string-append "--enable-ruby-binding=" (assoc-ref %outputs "ruby")) (string-append "--enable-python-binding=" (assoc-ref %outputs "python"))) #:phases (modify-phases %standard-phases (add-before 'check 'set-SHELL-variable (lambda _ ;; generator_manager.hpp either uses /bin/sh or $SHELL ;; to run tests. (setenv "SHELL" (which "bash"))))))) (native-inputs `(("bc" ,bc) ("time" ,time) ("ruby" ,ruby) ("python" ,python-wrapper) ("pkg-config" ,pkg-config))) (inputs `(("htslib" ,htslib))) (synopsis "Tool for fast counting of k-mers in DNA") (description "Jellyfish is a tool for fast, memory-efficient counting of k-mers in DNA. A k-mer is a substring of length k, and counting the occurrences of all such substrings is a central step in many analyses of DNA sequence. Jellyfish is a command-line program that reads FASTA and multi-FASTA files containing DNA sequences. It outputs its k-mer counts in a binary format, which can be translated into a human-readable text format using the @code{jellyfish dump} command, or queried for specific k-mers with @code{jellyfish query}.") (home-page "http://www.genome.umd.edu/jellyfish.html") ;; JELLYFISH seems to be 64-bit only. (supported-systems '("x86_64-linux" "aarch64-linux" "mips64el-linux")) ;; One of these licenses may be picked (license (list license:gpl3+ license:bsd-3)))) (define-public khmer (package (name "khmer") (version "3.0.0a3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/dib-lab/khmer") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "01l4jczglkl7yfhgvzx8j0df7k54bk1r8sli9ll16i1mis0d8f37")) (modules '((guix build utils))) (snippet '(begin ;; Delete bundled libraries. We do not replace the bundled seqan ;; as it is a modified subset of the old version 1.4.1. ;; ;; We do not replace the bundled MurmurHash as the canonical ;; repository for this code 'SMHasher' is unsuitable for providing ;; a library. See ;; https://lists.gnu.org/archive/html/guix-devel/2016-06/msg00977.html (delete-file-recursively "third-party/zlib") (delete-file-recursively "third-party/bzip2") (delete-file-recursively "third-party/seqan") (substitute* "setup.cfg" (("# libraries = z,bz2") "libraries = z,bz2") (("include:third-party/zlib:third-party/bzip2") "include:")) #t)))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'set-cc (lambda _ (setenv "CC" "gcc") #t)) (add-before 'reset-gzip-timestamps 'make-files-writable (lambda* (#:key outputs #:allow-other-keys) ;; Make sure .gz files are writable so that the ;; 'reset-gzip-timestamps' phase can do its work. (let ((out (assoc-ref outputs "out"))) (for-each make-file-writable (find-files out "\\.gz$")) #t)))))) (native-inputs `(("python-cython" ,python-cython) ("python-pytest" ,python-pytest) ("python-pytest-runner" ,python-pytest-runner))) (inputs `(("zlib" ,zlib) ("bzip2" ,bzip2) ("seqan" ,seqan-1) ("python-screed" ,python-screed) ("python-bz2file" ,python-bz2file))) (home-page "https://khmer.readthedocs.org/") (synopsis "K-mer counting, filtering and graph traversal library") (description "The khmer software is a set of command-line tools for working with DNA shotgun sequencing data from genomes, transcriptomes, metagenomes and single cells. Khmer can make de novo assemblies faster, and sometimes better. Khmer can also identify and fix problems with shotgun data.") ;; When building on i686, armhf and mips64el, we get the following error: ;; error: ['khmer', 'khmer.tests', 'oxli'] require 64-bit operating system (supported-systems '("x86_64-linux" "aarch64-linux")) (license license:bsd-3))) (define-public kaiju (package (name "kaiju") (version "1.6.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/bioinformatics-centre/kaiju") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "119pzi0ddzv9mjg4wwa6han0cwr3k3ssn7kirvsjfcq05mi5ka0x")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; There are no tests. #:phases (modify-phases %standard-phases (delete 'configure) (add-before 'build 'move-to-src-dir (lambda _ (chdir "src") #t)) (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p bin) (chdir "..") (copy-recursively "bin" bin)) #t))))) (inputs `(("perl" ,perl) ("zlib" ,zlib))) (home-page "http://kaiju.binf.ku.dk/") (synopsis "Fast and sensitive taxonomic classification for metagenomics") (description "Kaiju is a program for sensitive taxonomic classification of high-throughput sequencing reads from metagenomic whole genome sequencing experiments.") (license license:gpl3+))) (define-public macs (package (name "macs") (version "2.2.7.1") (source (origin ;; The PyPi tarball does not contain tests. (method git-fetch) (uri (git-reference (url "https://github.com/macs3-project/MACS") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "08zsgh65xbpv1md2s3wqmrk9g2mz6izmn59ryw5lbac54120p291")) (modules '((guix build utils))) ;; Remove files generated by Cython (snippet '(begin (for-each (lambda (file) (let ((generated-file (string-append (string-drop-right file 3) "c"))) (when (file-exists? generated-file) (delete-file generated-file)))) (find-files "." "\\.pyx$")) (delete-file "MACS2/IO/CallPeakUnitPrecompiled.c"))))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (replace 'check (lambda* (#:key tests? inputs outputs #:allow-other-keys) (when tests? (add-installed-pythonpath inputs outputs) (invoke "pytest" "-v"))))))) (inputs `(("python-numpy" ,python-numpy))) (native-inputs `(("python-cython" ,python-cython) ("python-pytest" ,python-pytest))) (home-page "https://github.com/macs3-project/MACS") (synopsis "Model based analysis for ChIP-Seq data") (description "MACS is an implementation of a ChIP-Seq analysis algorithm for identifying transcript factor binding sites named Model-based Analysis of ChIP-Seq (MACS). MACS captures the influence of genome complexity to evaluate the significance of enriched ChIP regions and it improves the spatial resolution of binding sites through combining the information of both sequencing tag position and orientation.") (license license:bsd-3))) (define-public mafft (package (name "mafft") (version "7.475") (source (origin (method url-fetch) (uri (string-append "https://mafft.cbrc.jp/alignment/software/mafft-" version "-without-extensions-src.tgz")) (file-name (string-append name "-" version ".tgz")) (sha256 (base32 "0i2i2m3blh2xkbkdk48hxfssks30ny0v381gdl7zwhcvp0axs26r")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no automated tests, though there are tests in the read me #:make-flags (let ((out (assoc-ref %outputs "out"))) (list (string-append "PREFIX=" out) (string-append "BINDIR=" (string-append out "/bin")))) #:phases (modify-phases %standard-phases (add-after 'unpack 'enter-dir (lambda _ (chdir "core") #t)) (add-after 'enter-dir 'patch-makefile (lambda _ ;; on advice from the MAFFT authors, there is no need to ;; distribute mafft-profile, mafft-distance, or ;; mafft-homologs.rb as they are too "specialised". (substitute* "Makefile" ;; remove mafft-homologs.rb from SCRIPTS (("^SCRIPTS = mafft mafft-homologs.rb") "SCRIPTS = mafft") ;; remove mafft-homologs from MANPAGES (("^MANPAGES = mafft.1 mafft-homologs.1") "MANPAGES = mafft.1") ;; remove mafft-distance from PROGS (("^PROGS = dvtditr dndfast7 dndblast sextet5 mafft-distance") "PROGS = dvtditr dndfast7 dndblast sextet5") ;; remove mafft-profile from PROGS (("splittbfast disttbfast tbfast mafft-profile 2cl mccaskillwrap") "splittbfast disttbfast tbfast f2cl mccaskillwrap") (("^rm -f mafft-profile mafft-profile.exe") "#") (("^rm -f mafft-distance mafft-distance.exe") ")#") ;; do not install MAN pages in libexec folder (("^\t\\$\\(INSTALL\\) -m 644 \\$\\(MANPAGES\\) \ \\$\\(DESTDIR\\)\\$\\(LIBDIR\\)") "#")) #t)) (add-after 'enter-dir 'patch-paths (lambda* (#:key inputs #:allow-other-keys) (substitute* '("pairash.c" "mafft.tmpl") (("perl") (which "perl")) (("([\"`| ])awk" _ prefix) (string-append prefix (which "awk"))) (("grep") (which "grep"))) #t)) (delete 'configure) (add-after 'install 'wrap-programs (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (path (string-append (assoc-ref %build-inputs "coreutils") "/bin:"))) (for-each (lambda (file) (wrap-program file `("PATH" ":" prefix (,path)))) (find-files bin))) #t))))) (inputs `(("perl" ,perl) ("ruby" ,ruby) ("gawk" ,gawk) ("grep" ,grep) ("coreutils" ,coreutils))) (home-page "https://mafft.cbrc.jp/alignment/software/") (synopsis "Multiple sequence alignment program") (description "MAFFT offers a range of multiple alignment methods for nucleotide and protein sequences. For instance, it offers L-INS-i (accurate; for alignment of <~200 sequences) and FFT-NS-2 (fast; for alignment of <~30,000 sequences).") (license (license:non-copyleft "https://mafft.cbrc.jp/alignment/software/license.txt" "BSD-3 with different formatting")))) (define-public mash (package (name "mash") (version "2.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/marbl/mash") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "049hwcc059p2fd9vwndn63laifvvsi0wmv84i6y1fr79k15dxwy6")) (modules '((guix build utils))) (snippet '(begin ;; Delete bundled kseq. ;; TODO: Also delete bundled murmurhash and open bloom filter. (delete-file "src/mash/kseq.h") #t)))) (build-system gnu-build-system) (arguments `(#:tests? #f ; No tests. #:configure-flags (list (string-append "--with-capnp=" (assoc-ref %build-inputs "capnproto")) (string-append "--with-gsl=" (assoc-ref %build-inputs "gsl"))) #:make-flags (list "CC=gcc") #:phases (modify-phases %standard-phases (add-after 'unpack 'fix-includes (lambda _ (substitute* '("src/mash/Sketch.cpp" "src/mash/CommandFind.cpp" "src/mash/CommandScreen.cpp") (("^#include \"kseq\\.h\"") "#include \"htslib/kseq.h\"")) #t)) (add-after 'fix-includes 'use-c++14 (lambda _ ;; capnproto 0.7 requires c++14 to build (substitute* "configure.ac" (("c\\+\\+11") "c++14")) (substitute* "Makefile.in" (("c\\+\\+11") "c++14")) #t))))) (native-inputs `(("autoconf" ,autoconf) ;; Capnproto and htslib are statically embedded in the final ;; application. Therefore we also list their licenses, below. ("capnproto" ,capnproto) ("htslib" ,htslib))) (inputs `(("gsl" ,gsl) ("zlib" ,zlib))) (supported-systems '("x86_64-linux")) (home-page "https://mash.readthedocs.io") (synopsis "Fast genome and metagenome distance estimation using MinHash") (description "Mash is a fast sequence distance estimator that uses the MinHash algorithm and is designed to work with genomes and metagenomes in the form of assemblies or reads.") (license (list license:bsd-3 ; Mash license:expat ; HTSlib and capnproto license:public-domain ; MurmurHash 3 license:cpl1.0)))) ; Open Bloom Filter (define-public metabat (package (name "metabat") (version "2.12.1") (source (origin (method git-fetch) (uri (git-reference (url "https://bitbucket.org/berkeleylab/metabat.git") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0hyg2smw1nz69mfvjpk45xyyychmda92c80a0cv7baji84ri4iyn")) (patches (search-patches "metabat-fix-compilation.patch")))) (build-system scons-build-system) (arguments `(#:scons ,scons-python2 #:scons-flags (list (string-append "PREFIX=" (assoc-ref %outputs "out")) (string-append "BOOST_ROOT=" (assoc-ref %build-inputs "boost"))) #:tests? #f ;; Tests are run during the build phase. #:phases (modify-phases %standard-phases (add-after 'unpack 'fix-includes (lambda _ (substitute* "src/BamUtils.h" (("^#include \"bam/bam\\.h\"") "#include \"samtools/bam.h\"") (("^#include \"bam/sam\\.h\"") "#include \"samtools/sam.h\"")) (substitute* "src/KseqReader.h" (("^#include \"bam/kseq\\.h\"") "#include \"htslib/kseq.h\"")) #t)) (add-after 'unpack 'fix-scons (lambda* (#:key inputs #:allow-other-keys) (substitute* "SConstruct" (("^htslib_dir += 'samtools'") (string-append "htslib_dir = '" (assoc-ref inputs "htslib") "'")) (("^samtools_dir = 'samtools'") (string-append "samtools_dir = '" (assoc-ref inputs "samtools") "'")) (("^findStaticOrShared\\('bam', hts_lib") (string-append "findStaticOrShared('bam', '" (assoc-ref inputs "samtools") "/lib'")) ;; Do not distribute README. (("^env\\.Install\\(idir_prefix, 'README\\.md'\\)") "")) #t))))) (inputs `(("zlib" ,zlib) ("perl" ,perl) ("samtools" ,samtools) ("htslib" ,htslib) ("boost" ,boost))) (home-page "https://bitbucket.org/berkeleylab/metabat") (synopsis "Reconstruction of single genomes from complex microbial communities") (description "Grouping large genomic fragments assembled from shotgun metagenomic sequences to deconvolute complex microbial communities, or metagenome binning, enables the study of individual organisms and their interactions. MetaBAT is an automated metagenome binning software, which integrates empirical probabilistic distances of genome abundance and tetranucleotide frequency.") ;; The source code contains inline assembly. (supported-systems '("x86_64-linux" "i686-linux")) (license (license:non-copyleft "file://license.txt" "See license.txt in the distribution.")))) (define-public minced (package (name "minced") (version "0.3.2") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ctSkennerton/minced") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1f5h9him0gd355cnx7p6pnxpknhckd4g0v62mg8zyhfbx9as25fv")))) (build-system gnu-build-system) (arguments `(#:test-target "test" #:phases (modify-phases %standard-phases (delete 'configure) (add-before 'check 'fix-test (lambda _ ;; Fix test for latest version. (substitute* "t/Aquifex_aeolicus_VF5.expected" (("minced:0.1.6") "minced:0.2.0")) #t)) (replace 'install ; No install target. (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (wrapper (string-append bin "/minced"))) ;; Minced comes with a wrapper script that tries to figure out where ;; it is located before running the JAR. Since these paths are known ;; to us, we build our own wrapper to avoid coreutils dependency. (install-file "minced.jar" bin) (with-output-to-file wrapper (lambda _ (display (string-append "#!" (assoc-ref inputs "bash") "/bin/sh\n\n" (assoc-ref inputs "jre") "/bin/java -jar " bin "/minced.jar \"$@\"\n")))) (chmod wrapper #o555)) #t))))) (native-inputs `(("jdk" ,icedtea "jdk"))) (inputs `(("bash" ,bash) ("jre" ,icedtea "out"))) (home-page "https://github.com/ctSkennerton/minced") (synopsis "Mining CRISPRs in Environmental Datasets") (description "MinCED is a program to find Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in DNA sequences. It can be used for unassembled metagenomic reads, but is mainly designed for full genomes and assembled metagenomic sequence.") (license license:gpl3+))) (define-public miso (package (name "miso") (version "0.5.4") (source (origin (method url-fetch) (uri (pypi-uri "misopy" version)) (sha256 (base32 "1z3x0vd8ma7pdrnywj7i3kgwl89sdkwrrn62zl7r5calqaq2hyip")) (modules '((guix build utils))) (snippet '(begin (substitute* "setup.py" ;; Use setuptools, or else the executables are not ;; installed. (("distutils.core") "setuptools") ;; Use "gcc" instead of "cc" for compilation. (("^defines") "cc.set_executables( compiler='gcc', compiler_so='gcc', linker_exe='gcc', linker_so='gcc -shared'); defines")) #t)))) (build-system python-build-system) (arguments `(#:python ,python-2 ; only Python 2 is supported #:tests? #f)) ; no "test" target (inputs `(("samtools" ,samtools) ("python-numpy" ,python2-numpy) ("python-pysam" ,python2-pysam) ("python-scipy" ,python2-scipy) ("python-matplotlib" ,python2-matplotlib))) (native-inputs `(("python-mock" ,python2-mock) ; for tests ("python-pytz" ,python2-pytz))) ; for tests (home-page "https://www.genes.mit.edu/burgelab/miso/index.html") (synopsis "Mixture of Isoforms model for RNA-Seq isoform quantitation") (description "MISO (Mixture-of-Isoforms) is a probabilistic framework that quantitates the expression level of alternatively spliced genes from RNA-Seq data, and identifies differentially regulated isoforms or exons across samples. By modeling the generative process by which reads are produced from isoforms in RNA-Seq, the MISO model uses Bayesian inference to compute the probability that a read originated from a particular isoform.") (license license:gpl2))) (define-public muscle (package (name "muscle") (version "3.8.1551") (source (origin (method url-fetch/tarbomb) (uri (string-append "http://www.drive5.com/muscle/muscle_src_" version ".tar.gz")) (sha256 (base32 "0bj8kj7sdizy3987zx6w7axihk40fk8rn76mpbqqjcnd64i5a367")))) (build-system gnu-build-system) (arguments `(#:make-flags (list "LDLIBS = -lm") #:phases (modify-phases %standard-phases (delete 'configure) (replace 'check ;; There are no tests, so just test if it runs. (lambda _ (invoke "./muscle" "-version") #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin"))) (install-file "muscle" bin) #t)))))) (home-page "http://www.drive5.com/muscle") (synopsis "Multiple sequence alignment program") (description "MUSCLE aims to be a fast and accurate multiple sequence alignment program for nucleotide and protein sequences.") ;; License information found in 'muscle -h' and usage.cpp. (license license:public-domain))) (define-public newick-utils ;; There are no recent releases so we package from git. (let ((commit "da121155a977197cab9fbb15953ca1b40b11eb87")) (package (name "newick-utils") (version (string-append "1.6-1." (string-take commit 8))) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/tjunier/newick_utils") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "1hkw21rq1mwf7xp0rmbb2gqc0i6p11108m69i7mr7xcjl268pxnb")))) (build-system gnu-build-system) (inputs ;; XXX: TODO: Enable Lua and Guile bindings. ;; https://github.com/tjunier/newick_utils/issues/13 `(("libxml2" ,libxml2) ("flex" ,flex) ("bison" ,bison))) (native-inputs `(("autoconf" ,autoconf) ("automake" ,automake) ("libtool" ,libtool))) (synopsis "Programs for working with newick format phylogenetic trees") (description "Newick-utils is a suite of utilities for processing phylogenetic trees in Newick format. Functions include re-rooting, extracting subtrees, trimming, pruning, condensing, drawing (ASCII graphics or SVG).") (home-page "https://github.com/tjunier/newick_utils") (license license:bsd-3)))) (define-public orfm (package (name "orfm") (version "0.7.1") (source (origin (method url-fetch) (uri (string-append "https://github.com/wwood/OrfM/releases/download/v" version "/orfm-" version ".tar.gz")) (sha256 (base32 "16iigyr2gd8x0imzkk1dr3k5xsds9bpmwg31ayvjg0f4pir9rwqr")))) (build-system gnu-build-system) (inputs `(("zlib" ,zlib))) (native-inputs `(("ruby-bio-commandeer" ,ruby-bio-commandeer) ("ruby-rspec" ,ruby-rspec) ("ruby" ,ruby))) (synopsis "Simple and not slow open reading frame (ORF) caller") (description "An ORF caller finds stretches of DNA that, when translated, are not interrupted by stop codons. OrfM finds and prints these ORFs.") (home-page "https://github.com/wwood/OrfM") (license license:lgpl3+))) (define-public python2-pbcore (package (name "python2-pbcore") (version "1.2.10") (source (origin (method url-fetch) (uri (pypi-uri "pbcore" version)) (sha256 (base32 "1kjmv891d6qbpp4shhhvkl02ff4q5xlpnls2513sm2cjcrs52f1i")))) (build-system python-build-system) (arguments `(#:python ,python-2 ;pbcore < 2.0 requires Python 2.7 #:phases (modify-phases %standard-phases (add-after 'unpack 'remove-sphinx-dependency (lambda _ ;; Sphinx is only required for documentation tests, which ;; we do not run; furthermore it depends on python2-sphinx ;; which is no longer maintained. (substitute* "requirements-dev.txt" (("^sphinx") "")) #t))))) (propagated-inputs `(("python-cython" ,python2-cython) ("python-numpy" ,python2-numpy) ("python-pysam" ,python2-pysam) ("python-h5py" ,python2-h5py))) (native-inputs `(("python-nose" ,python2-nose) ("python-pyxb" ,python2-pyxb))) (home-page "https://pacificbiosciences.github.io/pbcore/") (synopsis "Library for reading and writing PacBio data files") (description "The pbcore package provides Python APIs for interacting with PacBio data files and writing bioinformatics applications.") (license license:bsd-3))) (define-public python2-warpedlmm (package (name "python2-warpedlmm") (version "0.21") (source (origin (method url-fetch) (uri (pypi-uri "WarpedLMM" version ".zip")) (sha256 (base32 "1agfz6zqa8nc6cw47yh0s3y14gkpa9wqazwcj7mwwj3ffnw39p3j")))) (build-system python-build-system) (arguments `(#:python ,python-2 ; requires Python 2.7 #:tests? #f ; test data are not included #:phases (modify-phases %standard-phases (add-after 'unpack 'use-weave (lambda _ (substitute* "warpedlmm/util/linalg.py" (("from scipy import linalg, weave") "from scipy import linalg\nimport weave")) #t))))) (propagated-inputs `(("python-scipy" ,python2-scipy) ("python-numpy" ,python2-numpy) ("python-matplotlib" ,python2-matplotlib) ("python-fastlmm" ,python2-fastlmm) ("python-pandas" ,python2-pandas) ("python-pysnptools" ,python2-pysnptools) ("python-weave" ,python2-weave))) (native-inputs `(("python-mock" ,python2-mock) ("python-nose" ,python2-nose) ("unzip" ,unzip))) (home-page "https://github.com/PMBio/warpedLMM") (synopsis "Implementation of warped linear mixed models") (description "WarpedLMM is a Python implementation of the warped linear mixed model, which automatically learns an optimal warping function (or transformation) for the phenotype as it models the data.") (license license:asl2.0))) (define-public pbtranscript-tofu (let ((commit "8f5467fe6a4472bcfb4226c8720993c8507adfe4")) (package (name "pbtranscript-tofu") (version (string-append "2.2.3." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/PacificBiosciences/cDNA_primer") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "1lgnpi35ihay42qx0b6yl3kkgra723i413j33kvs0kvs61h82w0f")) (modules '((guix build utils))) (snippet '(begin ;; remove bundled Cython sources (delete-file "pbtranscript-tofu/pbtranscript/Cython-0.20.1.tar.gz") #t)))) (build-system python-build-system) (arguments `(#:python ,python-2 ;; FIXME: Tests fail with "No such file or directory: ;; pbtools/pbtranscript/modified_bx_intervals/intersection_unique.so" #:tests? #f #:phases (modify-phases %standard-phases (add-after 'unpack 'enter-directory (lambda _ (chdir "pbtranscript-tofu/pbtranscript/") #t)) ;; With setuptools version 18.0 and later this setup.py hack causes ;; a build error, so we disable it. (add-after 'enter-directory 'patch-setuppy (lambda _ (substitute* "setup.py" (("if 'setuptools.extension' in sys.modules:") "if False:")) #t))))) (inputs `(("python-numpy" ,python2-numpy) ("python-bx-python" ,python2-bx-python) ("python-networkx" ,python2-networkx) ("python-scipy" ,python2-scipy) ("python-pbcore" ,python2-pbcore) ("python-h5py" ,python2-h5py))) (native-inputs `(("python-cython" ,python2-cython) ("python-nose" ,python2-nose))) (home-page "https://github.com/PacificBiosciences/cDNA_primer") (synopsis "Analyze transcriptome data generated with the Iso-Seq protocol") (description "pbtranscript-tofu contains scripts to analyze transcriptome data generated using the PacBio Iso-Seq protocol.") (license license:bsd-3)))) (define-public prank (package (name "prank") (version "170427") (source (origin (method url-fetch) (uri (string-append "http://wasabiapp.org/download/prank/prank.source." version ".tgz")) (sha256 (base32 "0nc8g9c5rkdxcir46s0in9ci1sxwzbjibxrvkksf22ybnplvagk2")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'enter-src-dir (lambda _ (chdir "src") #t)) (add-after 'unpack 'remove-m64-flag ;; Prank will build with the correct 'bit-ness' without this flag ;; and this allows building on 32-bit machines. (lambda _ (substitute* "src/Makefile" (("-m64") "")) #t)) (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (man (string-append out "/share/man/man1")) (path (string-append (assoc-ref %build-inputs "mafft") "/bin:" (assoc-ref %build-inputs "exonerate") "/bin:" (assoc-ref %build-inputs "bppsuite") "/bin"))) (install-file "prank" bin) (wrap-program (string-append bin "/prank") `("PATH" ":" prefix (,path))) (install-file "prank.1" man)) #t))))) (inputs `(("mafft" ,mafft) ("exonerate" ,exonerate) ("bppsuite" ,bppsuite))) (home-page "http://wasabiapp.org/software/prank/") (synopsis "Probabilistic multiple sequence alignment program") (description "PRANK is a probabilistic multiple sequence alignment program for DNA, codon and amino-acid sequences. It is based on a novel algorithm that treats insertions correctly and avoids over-estimation of the number of deletion events. In addition, PRANK borrows ideas from maximum likelihood methods used in phylogenetics and correctly takes into account the evolutionary distances between sequences. Lastly, PRANK allows for defining a potential structure for sequences to be aligned and then, simultaneously with the alignment, predicts the locations of structural units in the sequences.") (license license:gpl2+))) (define-public proteinortho (package (name "proteinortho") (version "6.0.14") (source (origin (method git-fetch) (uri (git-reference (url "https://gitlab.com/paulklemm_PHD/proteinortho.git") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0pmy617zy2z2w6hjqxjhf3rzikf5n3mpia80ysq8233vfr7wrzff")) (modules '((guix build utils))) (snippet '(begin ;; remove pre-built scripts (delete-file-recursively "src/BUILD/") #t)))) (build-system gnu-build-system) (arguments `(#:test-target "test" #:make-flags '("CC=gcc") #:phases (modify-phases %standard-phases (replace 'configure ;; There is no configure script, so we modify the Makefile directly. (lambda* (#:key outputs #:allow-other-keys) (substitute* "Makefile" (("INSTALLDIR=.*") (string-append "INSTALLDIR=" (assoc-ref outputs "out") "/bin\n"))) #t)) (add-before 'install 'make-install-directory ;; The install directory is not created during 'make install'. (lambda* (#:key outputs #:allow-other-keys) (mkdir-p (string-append (assoc-ref outputs "out") "/bin")) #t)) (add-after 'install 'wrap-programs (lambda* (#:key inputs outputs #:allow-other-keys) (let ((path (getenv "PATH")) (out (assoc-ref outputs "out"))) (for-each (lambda (script) (wrap-script script `("PATH" ":" prefix (,path)))) (cons (string-append out "/bin/proteinortho") (find-files out "\\.(pl|py)$")))) #t))))) (inputs `(("guile" ,guile-3.0) ; for wrap-script ("diamond" ,diamond) ("perl" ,perl) ("python" ,python-wrapper) ("blast+" ,blast+) ("lapack" ,lapack) ("openblas" ,openblas))) (native-inputs `(("which" ,which))) (home-page "http://www.bioinf.uni-leipzig.de/Software/proteinortho") (synopsis "Detect orthologous genes across species") (description "Proteinortho is a tool to detect orthologous genes across different species. For doing so, it compares similarities of given gene sequences and clusters them to find significant groups. The algorithm was designed to handle large-scale data and can be applied to hundreds of species at once.") (license license:gpl3+))) (define-public pyicoteo (package (name "pyicoteo") (version "2.0.7") (source (origin (method git-fetch) (uri (git-reference (url "https://bitbucket.org/regulatorygenomicsupf/pyicoteo.git") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0hz5g8d25lbjy1wpscr490l0lmyvaix893hhax4fxnh1h9w34w8p")))) (build-system python-build-system) (arguments `(#:python ,python-2 ; does not work with Python 3 #:tests? #f)) ; there are no tests (inputs `(("python2-matplotlib" ,python2-matplotlib))) (home-page "https://bitbucket.org/regulatorygenomicsupf/pyicoteo") (synopsis "Analyze high-throughput genetic sequencing data") (description "Pyicoteo is a suite of tools for the analysis of high-throughput genetic sequencing data. It works with genomic coordinates. There are currently six different command-line tools: @enumerate @item pyicoregion: for generating exploratory regions automatically; @item pyicoenrich: for differential enrichment between two conditions; @item pyicoclip: for calling CLIP-Seq peaks without a control; @item pyicos: for genomic coordinates manipulation; @item pyicoller: for peak calling on punctuated ChIP-Seq; @item pyicount: to count how many reads from N experiment files overlap in a region file; @item pyicotrocol: to combine operations from pyicoteo. @end enumerate\n") (license license:gpl3+))) (define-public prodigal (package (name "prodigal") ;; Check for a new home page when updating this package: ;; https://github.com/hyattpd/Prodigal/issues/36#issuecomment-536617588 (version "2.6.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/hyattpd/Prodigal") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1fs1hqk83qjbjhrvhw6ni75zakx5ki1ayy3v6wwkn3xvahc9hi5s")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no check target #:make-flags (list (string-append "INSTALLDIR=" (assoc-ref %outputs "out") "/bin")) #:phases (modify-phases %standard-phases (delete 'configure)))) (home-page "https://github.com/hyattpd/Prodigal") (synopsis "Protein-coding gene prediction for Archaea and Bacteria") (description "Prodigal runs smoothly on finished genomes, draft genomes, and metagenomes, providing gene predictions in GFF3, Genbank, or Sequin table format. It runs quickly, in an unsupervised fashion, handles gaps, handles partial genes, and identifies translation initiation sites.") (license license:gpl3+))) (define-public roary (package (name "roary") (version "3.12.0") (source (origin (method url-fetch) (uri (string-append "mirror://cpan/authors/id/A/AJ/AJPAGE/Bio-Roary-" version ".tar.gz")) (sha256 (base32 "0qxrds9wx7cfhlkihrp6697kx0flhhxymap9fwan0b3rbdhcnmff")))) (build-system perl-build-system) (arguments `(#:phases (modify-phases %standard-phases (delete 'configure) (delete 'build) (replace 'check (lambda _ ;; The tests are not run by default, so we run each test file ;; directly. (setenv "PATH" (string-append (getcwd) "/bin" ":" (getenv "PATH"))) (setenv "PERL5LIB" (string-append (getcwd) "/lib" ":" (getenv "PERL5LIB"))) (for-each (lambda (file) (display file)(display "\n") (invoke "perl" file)) (find-files "t" ".*\\.t$")) #t)) (replace 'install ;; There is no 'install' target in the Makefile. (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (perl (string-append out "/lib/perl5/site_perl")) (roary-plots "contrib/roary_plots")) (mkdir-p bin) (mkdir-p perl) (copy-recursively "bin" bin) (copy-recursively "lib" perl) #t))) (add-after 'install 'wrap-programs (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (perl5lib (getenv "PERL5LIB")) (path (getenv "PATH"))) (for-each (lambda (prog) (let ((binary (string-append out "/" prog))) (wrap-program binary `("PERL5LIB" ":" prefix (,(string-append perl5lib ":" out "/lib/perl5/site_perl")))) (wrap-program binary `("PATH" ":" prefix (,(string-append path ":" out "/bin")))))) (find-files "bin" ".*[^R]$")) (let ((file (string-append out "/bin/roary-create_pan_genome_plots.R")) (r-site-lib (getenv "R_LIBS_SITE")) (coreutils-path (string-append (assoc-ref inputs "coreutils") "/bin"))) (wrap-program file `("R_LIBS_SITE" ":" prefix (,(string-append r-site-lib ":" out "/site-library/")))) (wrap-program file `("PATH" ":" prefix (,(string-append coreutils-path ":" out "/bin")))))) #t))))) (native-inputs `(("perl-env-path" ,perl-env-path) ("perl-test-files" ,perl-test-files) ("perl-test-most" ,perl-test-most) ("perl-test-output" ,perl-test-output))) (inputs `(("perl-array-utils" ,perl-array-utils) ("bioperl" ,bioperl-minimal) ("perl-digest-md5-file" ,perl-digest-md5-file) ("perl-exception-class" ,perl-exception-class) ("perl-file-find-rule" ,perl-file-find-rule) ("perl-file-grep" ,perl-file-grep) ("perl-file-slurper" ,perl-file-slurper) ("perl-file-which" ,perl-file-which) ("perl-graph" ,perl-graph) ("perl-graph-readwrite" ,perl-graph-readwrite) ("perl-log-log4perl" ,perl-log-log4perl) ("perl-moose" ,perl-moose) ("perl-perlio-utf8_strict" ,perl-perlio-utf8_strict) ("perl-text-csv" ,perl-text-csv) ("bedtools" ,bedtools) ("cd-hit" ,cd-hit) ("blast+" ,blast+) ("mcl" ,mcl) ("parallel" ,parallel) ("prank" ,prank) ("mafft" ,mafft) ("fasttree" ,fasttree) ("grep" ,grep) ("sed" ,sed) ("gawk" ,gawk) ("r-minimal" ,r-minimal) ("r-ggplot2" ,r-ggplot2) ("coreutils" ,coreutils))) (home-page "https://sanger-pathogens.github.io/Roary/") (synopsis "High speed stand-alone pan genome pipeline") (description "Roary is a high speed stand alone pan genome pipeline, which takes annotated assemblies in GFF3 format (produced by the Prokka program) and calculates the pan genome. Using a standard desktop PC, it can analyse datasets with thousands of samples, without compromising the quality of the results. 128 samples can be analysed in under 1 hour using 1 GB of RAM and a single processor. Roary is not intended for metagenomics or for comparing extremely diverse sets of genomes.") (license license:gpl3))) (define-public raxml (package (name "raxml") (version "8.2.12") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/stamatak/standard-RAxML") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1jqjzhch0rips0vp04prvb8vmc20c5pdmsqn8knadcf91yy859fh")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; There are no tests. ;; Use 'standard' Makefile rather than SSE or AVX ones. #:make-flags (list "-f" "Makefile.HYBRID.gcc") #:phases (modify-phases %standard-phases (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (executable "raxmlHPC-HYBRID")) (install-file executable bin) (symlink (string-append bin "/" executable) "raxml")) #t))))) (inputs `(("openmpi" ,openmpi))) (home-page "https://cme.h-its.org/exelixis/web/software/raxml/index.html") (synopsis "Randomized Axelerated Maximum Likelihood phylogenetic trees") (description "RAxML is a tool for phylogenetic analysis and post-analysis of large phylogenies.") ;; The source includes x86 specific code (supported-systems '("x86_64-linux" "i686-linux")) (license license:gpl2+))) (define-public rsem (package (name "rsem") (version "1.3.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/deweylab/RSEM") (commit (string-append "v" version)))) (sha256 (base32 "1jlq11d1p8qp64w75yj8cnbbd1a93viq10pzsbwal7vdn8fg13j1")) (file-name (git-file-name name version)) (modules '((guix build utils))) (snippet '(begin ;; remove bundled copy of boost and samtools (delete-file-recursively "boost") (delete-file-recursively "samtools-1.3") #t)))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no "check" target #:make-flags (list (string-append "BOOST=" (assoc-ref %build-inputs "boost") "/include/") (string-append "SAMHEADERS=" (assoc-ref %build-inputs "htslib") "/include/htslib/sam.h") (string-append "SAMLIBS=" (assoc-ref %build-inputs "htslib") "/lib/libhts.so")) #:phases (modify-phases %standard-phases ;; No "configure" script. ;; Do not build bundled samtools library. (replace 'configure (lambda _ (substitute* "Makefile" (("^all : \\$\\(PROGRAMS\\).*") "all: $(PROGRAMS)\n") (("^\\$\\(SAMLIBS\\).*") "")) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (string-append (assoc-ref outputs "out"))) (bin (string-append out "/bin/")) (perl (string-append out "/lib/perl5/site_perl"))) (mkdir-p bin) (mkdir-p perl) (for-each (lambda (file) (install-file file bin)) (find-files "." "rsem-.*")) (install-file "rsem_perl_utils.pm" perl)) #t)) (add-after 'install 'wrap-program (lambda* (#:key outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) (for-each (lambda (prog) (wrap-program (string-append out "/bin/" prog) `("PERL5LIB" ":" prefix (,(string-append out "/lib/perl5/site_perl"))))) '("rsem-calculate-expression" "rsem-control-fdr" "rsem-generate-data-matrix" "rsem-generate-ngvector" "rsem-plot-transcript-wiggles" "rsem-prepare-reference" "rsem-run-ebseq" "rsem-run-prsem-testing-procedure"))) #t))))) (inputs `(("boost" ,boost) ("r-minimal" ,r-minimal) ("perl" ,perl) ("htslib" ,htslib-1.3) ("zlib" ,zlib))) (home-page "http://deweylab.biostat.wisc.edu/rsem/") (synopsis "Estimate gene expression levels from RNA-Seq data") (description "RSEM is a software package for estimating gene and isoform expression levels from RNA-Seq data. The RSEM package provides a user-friendly interface, supports threads for parallel computation of the EM algorithm, single-end and paired-end read data, quality scores, variable-length reads and RSPD estimation. In addition, it provides posterior mean and 95% credibility interval estimates for expression levels. For visualization, it can generate BAM and Wiggle files in both transcript-coordinate and genomic-coordinate.") (license license:gpl3+))) (define-public rseqc (package (name "rseqc") (version "3.0.1") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/rseqc/" "RSeQC-" version ".tar.gz")) (sha256 (base32 "0gbb9iyb7swiv5455fm5rg98r7l6qn27v564yllqjd574hncpx6m")))) (build-system python-build-system) (inputs `(("python-cython" ,python-cython) ("python-bx-python" ,python-bx-python) ("python-pybigwig" ,python-pybigwig) ("python-pysam" ,python-pysam) ("python-numpy" ,python-numpy) ("zlib" ,zlib))) (native-inputs `(("python-nose" ,python-nose))) (home-page "http://rseqc.sourceforge.net/") (synopsis "RNA-seq quality control package") (description "RSeQC provides a number of modules that can comprehensively evaluate high throughput sequence data, especially RNA-seq data. Some basic modules inspect sequence quality, nucleotide composition bias, PCR bias and GC bias, while RNA-seq specific modules evaluate sequencing saturation, mapped reads distribution, coverage uniformity, strand specificity, etc.") (license license:gpl3+))) (define-public seek ;; There are no release tarballs. According to the installation ;; instructions at http://seek.princeton.edu/installation.jsp, the latest ;; stable release is identified by this changeset ID. (let ((changeset "2329130") (revision "1")) (package (name "seek") (version (string-append "0-" revision "." changeset)) (source (origin (method hg-fetch) (uri (hg-reference (url "https://bitbucket.org/libsleipnir/sleipnir") (changeset changeset))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "0qrvilwh18dpbhkf92qvxbmay0j75ra3jg2wrhz67gf538zzphsx")))) (build-system gnu-build-system) (arguments `(#:modules ((srfi srfi-1) (guix build gnu-build-system) (guix build utils)) #:phases (let ((dirs '("SeekMiner" "SeekEvaluator" "SeekPrep" "Distancer" "Data2DB" "PCL2Bin"))) (modify-phases %standard-phases (replace 'bootstrap (lambda _ (substitute* "gen_tools_am" (("/usr/bin/env.*") (which "perl"))) (invoke "bash" "gen_auto") #t)) (add-after 'build 'build-additional-tools (lambda* (#:key make-flags #:allow-other-keys) (for-each (lambda (dir) (with-directory-excursion (string-append "tools/" dir) (apply invoke "make" make-flags))) dirs) #t)) (add-after 'install 'install-additional-tools (lambda* (#:key make-flags #:allow-other-keys) (for-each (lambda (dir) (with-directory-excursion (string-append "tools/" dir) (apply invoke `("make" ,@make-flags "install")))) dirs) #t)))))) (inputs `(("gsl" ,gsl) ("boost" ,boost) ("libsvm" ,libsvm) ("readline" ,readline) ("gengetopt" ,gengetopt) ("log4cpp" ,log4cpp))) (native-inputs `(("autoconf" ,autoconf) ("automake" ,automake) ("perl" ,perl))) (home-page "http://seek.princeton.edu") (synopsis "Gene co-expression search engine") (description "SEEK is a computational gene co-expression search engine. SEEK provides biologists with a way to navigate the massive human expression compendium that now contains thousands of expression datasets. SEEK returns a robust ranking of co-expressed genes in the biological area of interest defined by the user's query genes. It also prioritizes thousands of expression datasets according to the user's query of interest.") (license license:cc-by3.0)))) (define-public samtools (package (name "samtools") (version "1.12") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/samtools/samtools/" version "/samtools-" version ".tar.bz2")) (sha256 (base32 "1jrdj2idpma5ja9cg0rr73b565vdbr9wyy6zig54bidicc2pg8vd")) (modules '((guix build utils))) (snippet '(begin ;; Delete bundled htslib. (delete-file-recursively "htslib-1.12") #t)))) (build-system gnu-build-system) (arguments `(#:modules ((ice-9 ftw) (ice-9 regex) (guix build gnu-build-system) (guix build utils)) #:configure-flags (list "--with-ncurses") #:phases (modify-phases %standard-phases (add-after 'unpack 'patch-tests (lambda _ (substitute* "test/test.pl" ;; The test script calls out to /bin/bash (("/bin/bash") (which "bash"))) #t)) (add-after 'install 'install-library (lambda* (#:key outputs #:allow-other-keys) (let ((lib (string-append (assoc-ref outputs "out") "/lib"))) (install-file "libbam.a" lib) #t))) (add-after 'install 'install-headers (lambda* (#:key outputs #:allow-other-keys) (let ((include (string-append (assoc-ref outputs "out") "/include/samtools/"))) (for-each (lambda (file) (install-file file include)) (scandir "." (lambda (name) (string-match "\\.h$" name)))) #t)))))) (native-inputs `(("pkg-config" ,pkg-config))) (inputs `(("htslib" ,htslib) ("ncurses" ,ncurses) ("perl" ,perl) ("python" ,python) ("zlib" ,zlib))) (home-page "http://samtools.sourceforge.net") (synopsis "Utilities to efficiently manipulate nucleotide sequence alignments") (description "Samtools implements various utilities for post-processing nucleotide sequence alignments in the SAM, BAM, and CRAM formats, including indexing, variant calling (in conjunction with bcftools), and a simple alignment viewer.") (license license:expat))) (define-public samtools-1.9 (package (inherit samtools) (name "samtools") (version "1.9") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/samtools/samtools/" version "/samtools-" version ".tar.bz2")) (sha256 (base32 "10ilqbmm7ri8z431sn90lvbjwizd0hhkf9rcqw8j823hf26nhgq8")) (modules '((guix build utils))) (snippet '(begin ;; Delete bundled htslib. (delete-file-recursively "htslib-1.9") #t)))) (inputs `(("htslib" ,htslib-1.9) ("ncurses" ,ncurses) ("perl" ,perl) ("python" ,python) ("zlib" ,zlib))))) (define-public samtools-0.1 ;; This is the most recent version of the 0.1 line of samtools. The input ;; and output formats differ greatly from that used and produced by samtools ;; 1.x and is still used in many bioinformatics pipelines. (package (inherit samtools) (version "0.1.19") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/samtools/samtools/" version "/samtools-" version ".tar.bz2")) (sha256 (base32 "1m33xsfwz0s8qi45lylagfllqg7fphf4dr0780rsvw75av9wk06h")))) (arguments `(#:tests? #f ;no "check" target #:make-flags (list "LIBCURSES=-lncurses") ,@(substitute-keyword-arguments (package-arguments samtools) ((#:phases phases) `(modify-phases ,phases (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p bin) (install-file "samtools" bin) #t))) (delete 'patch-tests) (delete 'configure)))))))) (define-public mosaik (let ((commit "5c25216d3522d6a33e53875cd76a6d65001e4e67")) (package (name "mosaik") (version "2.2.30") (source (origin ;; There are no release tarballs nor tags. (method git-fetch) (uri (git-reference (url "https://github.com/wanpinglee/MOSAIK") (commit commit))) (file-name (string-append name "-" version)) (sha256 (base32 "17gj3s07cm77r41z92awh0bim7w7q7fbn0sf5nkqmcm1vw052qgw")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no tests #:make-flags (list "CC=gcc") #:phases (modify-phases %standard-phases (replace 'configure (lambda _ (chdir "src") #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p bin) (copy-recursively "../bin" bin) #t)))))) (inputs `(("perl" ,perl) ("zlib:static" ,zlib "static") ("zlib" ,zlib))) (supported-systems '("x86_64-linux")) (home-page "https://github.com/wanpinglee/MOSAIK") (synopsis "Map nucleotide sequence reads to reference genomes") (description "MOSAIK is a program for mapping second and third-generation sequencing reads to a reference genome. MOSAIK can align reads generated by all the major sequencing technologies, including Illumina, Applied Biosystems SOLiD, Roche 454, Ion Torrent and Pacific BioSciences SMRT.") ;; MOSAIK is released under the GPLv2+ with the exception of third-party ;; code released into the public domain: ;; 1. fastlz by Ariya Hidayat - http://www.fastlz.org/ ;; 2. MD5 implementation - RSA Data Security, RFC 1321 (license (list license:gpl2+ license:public-domain))))) (define-public mosaicatcher (package (name "mosaicatcher") (version "0.3.1") (source (origin ;; There are no release tarballs nor tags. (method git-fetch) (uri (git-reference (url "https://github.com/friendsofstrandseq/mosaicatcher") (commit (string-append version "-dev")))) (file-name (git-file-name name version)) (sha256 (base32 "1n2s5wvvj2y0vfgjkg1q11xahpbagxz7h2vf5q7qyy25s12kbzbd")) (patches (search-patches "mosaicatcher-unbundle-htslib.patch")))) (build-system cmake-build-system) (arguments `(#:tests? #false ; there are no tests #:phases (modify-phases %standard-phases (add-after 'unpack 'chdir (lambda _ (chdir "src"))) (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((target (assoc-ref outputs "out")) (bin (string-append target "/bin")) (share (string-append target "/share/mosaicatcher"))) (install-file "mosaic" bin) (mkdir-p share) (copy-recursively "../R" share))))))) (inputs `(("boost" ,boost) ("htslib" ,htslib))) (home-page "https://github.com/friendsofstrandseq/mosaicatcher") (synopsis "Count and classify Strand-seq reads") (description "Mosaicatcher counts Strand-seq reads and classifies strand states of each chromosome in each cell using a Hidden Markov Model.") (license license:expat))) (define-public ngs-sdk (package (name "ngs-sdk") (version "2.10.5") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ncbi/ngs") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1ix51c25hjn57w93qmwzw80xh2i34wx8j2hn7szh8p6w8i3az5qa")))) (build-system gnu-build-system) (arguments `(#:parallel-build? #f ; not supported #:tests? #f ; no "check" target #:phases (modify-phases %standard-phases (replace 'configure (lambda* (#:key outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) ;; Allow 'konfigure.perl' to find 'package.prl'. (setenv "PERL5LIB" (string-append ".:" (getenv "PERL5LIB"))) ;; The 'configure' script doesn't recognize things like ;; '--enable-fast-install'. (invoke "./configure" (string-append "--build-prefix=" (getcwd) "/build") (string-append "--prefix=" out)) #t))) (add-after 'unpack 'enter-dir (lambda _ (chdir "ngs-sdk") #t))))) (native-inputs `(("perl" ,perl))) ;; According to the test ;; unless ($MARCH =~ /x86_64/i || $MARCH =~ /i?86/i) ;; in ngs-sdk/setup/konfigure.perl (supported-systems '("i686-linux" "x86_64-linux")) (home-page "https://github.com/ncbi/ngs") (synopsis "API for accessing Next Generation Sequencing data") (description "NGS is a domain-specific API for accessing reads, alignments and pileups produced from Next Generation Sequencing. The API itself is independent from any particular back-end implementation, and supports use of multiple back-ends simultaneously.") (license license:public-domain))) (define-public java-ngs (package (inherit ngs-sdk) (name "java-ngs") (arguments `(,@(substitute-keyword-arguments `(#:modules ((guix build gnu-build-system) (guix build utils) (srfi srfi-1) (srfi srfi-26)) ,@(package-arguments ngs-sdk)) ((#:phases phases) `(modify-phases ,phases (replace 'enter-dir (lambda _ (chdir "ngs-java") #t))))))) (inputs `(("jdk" ,icedtea "jdk") ("ngs-sdk" ,ngs-sdk))) (synopsis "Java bindings for NGS SDK"))) (define-public ncbi-vdb (package (name "ncbi-vdb") (version "2.10.6") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ncbi/ncbi-vdb") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "0m8hlxscidsfqm9x9fyi62q6lpf1dv5115kgjjgnrkl49q9c27m6")))) (build-system gnu-build-system) (arguments `(#:parallel-build? #f ; not supported #:tests? #f ; no "check" target #:make-flags '("HAVE_HDF5=1") #:phases (modify-phases %standard-phases (add-after 'unpack 'make-files-writable (lambda _ (for-each make-file-writable (find-files "." ".*")) #t)) (add-before 'configure 'set-perl-search-path (lambda _ ;; Work around "dotless @INC" build failure. (setenv "PERL5LIB" (string-append (getcwd) "/setup:" (getenv "PERL5LIB"))) #t)) ;; See https://github.com/ncbi/ncbi-vdb/issues/14 (add-after 'unpack 'patch-krypto-flags (lambda _ (substitute* "libs/krypto/Makefile" (("-Wa,-march=generic64\\+aes") "") (("-Wa,-march=generic64\\+sse4") "")) #t)) (replace 'configure (lambda* (#:key inputs outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) ;; Override include path for libmagic (substitute* "setup/package.prl" (("name => 'magic', Include => '/usr/include'") (string-append "name=> 'magic', Include => '" (assoc-ref inputs "libmagic") "/include" "'"))) ;; Install kdf5 library (needed by sra-tools) (substitute* "build/Makefile.install" (("LIBRARIES_TO_INSTALL =") "LIBRARIES_TO_INSTALL = kdf5.$(VERSION_LIBX) kdf5.$(VERSION_SHLX)")) (substitute* "build/Makefile.env" (("CFLAGS =" prefix) (string-append prefix "-msse2 "))) ;; Override search path for ngs-java (substitute* "setup/package.prl" (("/usr/local/ngs/ngs-java") (assoc-ref inputs "java-ngs"))) ;; The 'configure' script doesn't recognize things like ;; '--enable-fast-install'. (invoke "./configure" (string-append "--build-prefix=" (getcwd) "/build") (string-append "--prefix=" (assoc-ref outputs "out")) (string-append "--debug") (string-append "--with-xml2-prefix=" (assoc-ref inputs "libxml2")) (string-append "--with-ngs-sdk-prefix=" (assoc-ref inputs "ngs-sdk")) (string-append "--with-hdf5-prefix=" (assoc-ref inputs "hdf5"))) #t))) (add-after 'install 'install-interfaces (lambda* (#:key outputs #:allow-other-keys) ;; Install interface libraries. On i686 the interface libraries ;; are installed to "linux/gcc/i386", so we need to use the Linux ;; architecture name ("i386") instead of the target system prefix ;; ("i686"). (mkdir (string-append (assoc-ref outputs "out") "/ilib")) (copy-recursively (string-append "build/ncbi-vdb/linux/gcc/" ,(system->linux-architecture (or (%current-target-system) (%current-system))) "/rel/ilib") (string-append (assoc-ref outputs "out") "/ilib")) ;; Install interface headers (copy-recursively "interfaces" (string-append (assoc-ref outputs "out") "/include")) #t)) ;; These files are needed by sra-tools. (add-after 'install 'install-configuration-files (lambda* (#:key outputs #:allow-other-keys) (let ((target (string-append (assoc-ref outputs "out") "/kfg"))) (mkdir target) (install-file "libs/kfg/default.kfg" target) (install-file "libs/kfg/certs.kfg" target)) #t))))) (inputs `(("libxml2" ,libxml2) ("ngs-sdk" ,ngs-sdk) ("java-ngs" ,java-ngs) ("libmagic" ,file) ("hdf5" ,hdf5))) (native-inputs `(("perl" ,perl))) ;; NCBI-VDB requires SSE capability. (supported-systems '("i686-linux" "x86_64-linux")) (home-page "https://github.com/ncbi/ncbi-vdb") (synopsis "Database engine for genetic information") (description "The NCBI-VDB library implements a highly compressed columnar data warehousing engine that is most often used to store genetic information. Databases are stored in a portable image within the file system, and can be accessed/downloaded on demand across HTTP.") (license license:public-domain))) (define-public plink (package (name "plink") (version "1.07") (source (origin (method url-fetch) (uri (string-append "http://pngu.mgh.harvard.edu/~purcell/plink/dist/plink-" version "-src.zip")) (sha256 (base32 "0as8gxm4pjyc8dxmm1sl873rrd7wn5qs0l29nqfnl31x8i467xaa")) (patches (search-patches "plink-1.07-unclobber-i.patch" "plink-endian-detection.patch")))) (build-system gnu-build-system) (arguments '(#:tests? #f ;no "check" target #:make-flags (list (string-append "LIB_LAPACK=" (assoc-ref %build-inputs "lapack") "/lib/liblapack.so") "WITH_LAPACK=1" "FORCE_DYNAMIC=1" ;; disable phoning home "WITH_WEBCHECK=") #:phases (modify-phases %standard-phases ;; no "configure" script (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (install-file "plink" bin) #t)))))) (inputs `(("zlib" ,zlib) ("lapack" ,lapack))) (native-inputs `(("unzip" ,unzip))) (home-page "http://pngu.mgh.harvard.edu/~purcell/plink/") (synopsis "Whole genome association analysis toolset") (description "PLINK is a whole genome association analysis toolset, designed to perform a range of basic, large-scale analyses in a computationally efficient manner. The focus of PLINK is purely on analysis of genotype/phenotype data, so there is no support for steps prior to this (e.g. study design and planning, generating genotype or CNV calls from raw data). Through integration with gPLINK and Haploview, there is some support for the subsequent visualization, annotation and storage of results.") ;; Code is released under GPLv2, except for fisher.h, which is under ;; LGPLv2.1+ (license (list license:gpl2 license:lgpl2.1+)))) (define-public plink-ng (package (inherit plink) (name "plink-ng") (version "2.00a2.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/chrchang/plink-ng") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1p88lz9agzjlspjhciz61qjc36cfniv4nkxszyy0njqyc5rzc0cd")))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no "check" target #:make-flags (list "BLASFLAGS=-llapack -lopenblas" "CFLAGS=-Wall -O2 -DDYNAMIC_ZLIB=1" "ZLIB=-lz" "BIN=plink prettify" (string-append "CC=" ,(cc-for-target)) (string-append "PREFIX=" (assoc-ref %outputs "out")) "DESTDIR=") #:phases (modify-phases %standard-phases (add-after 'unpack 'chdir (lambda _ (chdir "1.9") #t)) (delete 'configure)))) ; no "configure" script (inputs `(("lapack" ,lapack) ("openblas" ,openblas) ("zlib" ,zlib))) (home-page "https://www.cog-genomics.org/plink/") (license license:gpl3+))) (define-public smithlab-cpp (let ((revision "1") (commit "728a097bec88c6f4b8528b685932049e660eff2e")) (package (name "smithlab-cpp") (version (string-append "0." revision "." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/smithlabcode/smithlab_cpp") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "0d476lmj312xk77kr9fzrv7z1bv96yfyx0w7y62ycmnfbx32ll74")))) (build-system gnu-build-system) (arguments `(#:modules ((guix build gnu-build-system) (guix build utils) (srfi srfi-26)) #:tests? #f ;no "check" target #:phases (modify-phases %standard-phases (add-after 'unpack 'use-samtools-headers (lambda _ (substitute* '("SAM.cpp" "SAM.hpp") (("sam.h") "samtools/sam.h")) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (lib (string-append out "/lib")) (include (string-append out "/include/smithlab-cpp"))) (mkdir-p lib) (mkdir-p include) (for-each (cut install-file <> lib) (find-files "." "\\.o$")) (for-each (cut install-file <> include) (find-files "." "\\.hpp$"))) #t)) (delete 'configure)))) (inputs `(("samtools" ,samtools-0.1) ("zlib" ,zlib))) (home-page "https://github.com/smithlabcode/smithlab_cpp") (synopsis "C++ helper library for functions used in Smith lab projects") (description "Smithlab CPP is a C++ library that includes functions used in many of the Smith lab bioinformatics projects, such as a wrapper around Samtools data structures, classes for genomic regions, mapped sequencing reads, etc.") (license license:gpl3+)))) (define-public preseq (package (name "preseq") (version "2.0.3") (source (origin (method url-fetch) (uri (string-append "https://github.com/smithlabcode/preseq/" "releases/download/v" version "/preseq_v" version ".tar.bz2")) (sha256 (base32 "149x9xmk1wy1gff85325yfzqc0qk4sgp1w6gbyj9cnji4x1dszbl")) (modules '((guix build utils))) (snippet '(begin ;; Remove bundled samtools. (delete-file-recursively "samtools") #t)))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no "check" target #:phases (modify-phases %standard-phases (delete 'configure)) #:make-flags (list (string-append "PREFIX=" (assoc-ref %outputs "out")) (string-append "LIBBAM=" (assoc-ref %build-inputs "samtools") "/lib/libbam.a") (string-append "SMITHLAB_CPP=" (assoc-ref %build-inputs "smithlab-cpp") "/lib") "PROGS=preseq" "INCLUDEDIRS=$(SMITHLAB_CPP)/../include/smithlab-cpp $(SAMTOOLS_DIR)"))) (inputs `(("gsl" ,gsl) ("samtools" ,samtools-0.1) ("smithlab-cpp" ,smithlab-cpp) ("zlib" ,zlib))) (home-page "http://smithlabresearch.org/software/preseq/") (synopsis "Program for analyzing library complexity") (description "The preseq package is aimed at predicting and estimating the complexity of a genomic sequencing library, equivalent to predicting and estimating the number of redundant reads from a given sequencing depth and how many will be expected from additional sequencing using an initial sequencing experiment. The estimates can then be used to examine the utility of further sequencing, optimize the sequencing depth, or to screen multiple libraries to avoid low complexity samples.") (license license:gpl3+))) (define-public python-screed (package (name "python-screed") (version "1.0") (source (origin (method url-fetch) (uri (pypi-uri "screed" version)) (sha256 (base32 "148vcb7w2wr6a4w6vs2bsxanbqibxfk490zbcbg4m61s8669zdjx")))) (build-system python-build-system) (arguments '(#:phases (modify-phases %standard-phases ;; Tests must be run after installation, as the "screed" command does ;; not exist right after building. (delete 'check) (add-after 'install 'check (lambda* (#:key inputs outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) (setenv "PYTHONPATH" (string-append out "/lib/python" (string-take (string-take-right (assoc-ref inputs "python") 5) 3) "/site-packages:" (getenv "PYTHONPATH"))) (setenv "PATH" (string-append out "/bin:" (getenv "PATH")))) (invoke "python" "setup.py" "test") #t))))) (native-inputs `(("python-pytest" ,python-pytest) ("python-pytest-cov" ,python-pytest-cov) ("python-pytest-runner" ,python-pytest-runner))) (inputs `(("python-bz2file" ,python-bz2file))) (home-page "https://github.com/dib-lab/screed/") (synopsis "Short read sequence database utilities") (description "Screed parses FASTA and FASTQ files and generates databases. Values such as sequence name, sequence description, sequence quality and the sequence itself can be retrieved from these databases.") (license license:bsd-3))) (define-public python2-screed (package-with-python2 python-screed)) (define-public sra-tools (package (name "sra-tools") (version "2.10.6") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ncbi/sra-tools") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1cr2mijkfs5sm35ffjs6861qsd1qkgnhnbavdv65zg5d655abbjf")))) (build-system gnu-build-system) (arguments `(#:parallel-build? #f ; not supported #:tests? #f ; no "check" target #:make-flags (list (string-append "DEFAULT_CRT=" (assoc-ref %build-inputs "ncbi-vdb") "/kfg/certs.kfg") (string-append "DEFAULT_KFG=" (assoc-ref %build-inputs "ncbi-vdb") "/kfg/default.kfg") (string-append "VDB_LIBDIR=" (assoc-ref %build-inputs "ncbi-vdb") ,(if (string-prefix? "x86_64" (or (%current-target-system) (%current-system))) "/lib64" "/lib32"))) #:phases (modify-phases %standard-phases (add-before 'configure 'set-perl-search-path (lambda _ ;; Work around "dotless @INC" build failure. (setenv "PERL5LIB" (string-append (getcwd) "/setup:" (getenv "PERL5LIB"))) #t)) (replace 'configure (lambda* (#:key inputs outputs #:allow-other-keys) ;; The build system expects a directory containing the sources and ;; raw build output of ncbi-vdb, including files that are not ;; installed. Since we are building against an installed version of ;; ncbi-vdb, the following modifications are needed. (substitute* "setup/konfigure.perl" ;; Make the configure script look for the "ilib" directory of ;; "ncbi-vdb" without first checking for the existence of a ;; matching library in its "lib" directory. (("^ my \\$f = File::Spec->catdir\\(\\$libdir, \\$lib\\);") "my $f = File::Spec->catdir($ilibdir, $ilib);") ;; Look for interface libraries in ncbi-vdb's "ilib" directory. (("my \\$ilibdir = File::Spec->catdir\\(\\$builddir, 'ilib'\\);") "my $ilibdir = File::Spec->catdir($dir, 'ilib');")) ;; Dynamic linking (substitute* "tools/copycat/Makefile" (("smagic-static") "lmagic")) (substitute* "tools/driver-tool/utf8proc/Makefile" (("CC\\?=gcc") "myCC=gcc") (("\\(CC\\)") "(myCC)")) ;; The 'configure' script doesn't recognize things like ;; '--enable-fast-install'. (invoke "./configure" (string-append "--build-prefix=" (getcwd) "/build") (string-append "--prefix=" (assoc-ref outputs "out")) (string-append "--debug") (string-append "--with-fuse-prefix=" (assoc-ref inputs "fuse")) (string-append "--with-magic-prefix=" (assoc-ref inputs "libmagic")) ;; TODO: building with libxml2 fails with linker errors #; (string-append "--with-xml2-prefix=" (assoc-ref inputs "libxml2")) (string-append "--with-ncbi-vdb-sources=" (assoc-ref inputs "ncbi-vdb")) (string-append "--with-ncbi-vdb-build=" (assoc-ref inputs "ncbi-vdb")) (string-append "--with-ngs-sdk-prefix=" (assoc-ref inputs "ngs-sdk")) (string-append "--with-hdf5-prefix=" (assoc-ref inputs "hdf5"))) #t))))) (native-inputs `(("perl" ,perl))) (inputs `(("ngs-sdk" ,ngs-sdk) ("ncbi-vdb" ,ncbi-vdb) ("libmagic" ,file) ("fuse" ,fuse) ("hdf5" ,hdf5-1.10) ("zlib" ,zlib) ("python" ,python-wrapper))) (home-page "https://trace.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?view=software") (synopsis "Tools and libraries for reading and writing sequencing data") (description "The SRA Toolkit from NCBI is a collection of tools and libraries for reading of sequencing files from the Sequence Read Archive (SRA) database and writing files into the .sra format.") (license license:public-domain))) (define-public seqan (package (name "seqan") (version "2.4.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/seqan/seqan/releases/" "download/seqan-v" version "/seqan-library-" version ".tar.xz")) (sha256 (base32 "19a1rlxx03qy1i1iriicly68w64yjxbv24g9gdywnfmq998v35yx")))) ;; The documentation is 7.8MB and the includes are 3.6MB heavy, so it ;; makes sense to split the outputs. (outputs '("out" "doc")) (build-system trivial-build-system) (arguments `(#:modules ((guix build utils)) #:builder (begin (use-modules (guix build utils)) (let ((tar (assoc-ref %build-inputs "tar")) (xz (assoc-ref %build-inputs "xz")) (out (assoc-ref %outputs "out")) (doc (assoc-ref %outputs "doc"))) (setenv "PATH" (string-append tar "/bin:" xz "/bin")) (invoke "tar" "xvf" (assoc-ref %build-inputs "source")) (chdir (string-append "seqan-library-" ,version)) (copy-recursively "include" (string-append out "/include")) (copy-recursively "share" (string-append doc "/share")) #t)))) (native-inputs `(("source" ,source) ("tar" ,tar) ("xz" ,xz))) (home-page "https://www.seqan.de") (synopsis "Library for nucleotide sequence analysis") (description "SeqAn is a C++ library of efficient algorithms and data structures for the analysis of sequences with the focus on biological data. It contains algorithms and data structures for string representation and their manipulation, online and indexed string search, efficient I/O of bioinformatics file formats, sequence alignment, and more.") (license license:bsd-3))) (define-public seqan-1 (package (inherit seqan) (name "seqan") (version "1.4.2") (source (origin (method url-fetch) (uri (string-append "https://packages.seqan.de/seqan-library/" "seqan-library-" version ".tar.bz2")) (sha256 (base32 "05s3wrrwn50f81aklfm65i4a749zag1vr8z03k21xm0pdxy47yvp")))) ;; The documentation is 7.8MB and the includes are 3.6MB heavy, so it ;; makes sense to split the outputs. (outputs '("out" "doc")) (build-system trivial-build-system) (arguments `(#:modules ((guix build utils)) #:builder (begin (use-modules (guix build utils)) (let ((tar (assoc-ref %build-inputs "tar")) (bzip (assoc-ref %build-inputs "bzip2")) (out (assoc-ref %outputs "out")) (doc (assoc-ref %outputs "doc"))) (setenv "PATH" (string-append tar "/bin:" bzip "/bin")) (invoke "tar" "xvf" (assoc-ref %build-inputs "source")) (chdir (string-append "seqan-library-" ,version)) (copy-recursively "include" (string-append out "/include")) (copy-recursively "share" (string-append doc "/share")) #t)))) (native-inputs `(("source" ,source) ("tar" ,tar) ("bzip2" ,bzip2))))) (define-public seqmagick (package (name "seqmagick") (version "0.8.0") (source (origin (method url-fetch) (uri (pypi-uri "seqmagick" version)) (sha256 (base32 "0pf98da7i59q47gwrbx0wjk6xlvbybiwphw80w7h4ydjj0579a2b")))) (build-system python-build-system) (inputs `(("python-biopython" ,python-biopython))) (native-inputs `(("python-nose" ,python-nose))) (home-page "https://github.com/fhcrc/seqmagick") (synopsis "Tools for converting and modifying sequence files") (description "Bioinformaticians often have to convert sequence files between formats and do little manipulations on them, and it's not worth writing scripts for that. Seqmagick is a utility to expose the file format conversion in BioPython in a convenient way. Instead of having a big mess of scripts, there is one that takes arguments.") (license license:gpl3))) (define-public seqtk (package (name "seqtk") (version "1.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/lh3/seqtk") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1bfzlqa84b5s1qi22blmmw2s8xdyp9h9ydcq22pfjhh5gab3yz6l")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (delete 'configure) (replace 'check ;; There are no tests, so we just run a sanity check. (lambda _ (invoke "./seqtk" "seq") #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (install-file "seqtk" bin) #t)))))) (inputs `(("zlib" ,zlib))) (home-page "https://github.com/lh3/seqtk") (synopsis "Toolkit for processing biological sequences in FASTA/Q format") (description "Seqtk is a fast and lightweight tool for processing sequences in the FASTA or FASTQ format. It parses both FASTA and FASTQ files which can be optionally compressed by gzip.") (license license:expat))) (define-public snap-aligner (package (name "snap-aligner") (version "1.0beta.18") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/amplab/snap") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "01w3qq4wm07z73vky0cfwlmrbf50n3w722cxrlzxfi99mnb808d8")))) (build-system gnu-build-system) (arguments '(#:phases (modify-phases %standard-phases (delete 'configure) (replace 'check (lambda _ (invoke "./unit_tests") #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin"))) (install-file "snap-aligner" bin) (install-file "SNAPCommand" bin) #t)))))) (native-inputs `(("zlib" ,zlib))) (home-page "http://snap.cs.berkeley.edu/") (synopsis "Short read DNA sequence aligner") (description "SNAP is a fast and accurate aligner for short DNA reads. It is optimized for modern read lengths of 100 bases or higher, and takes advantage of these reads to align data quickly through a hash-based indexing scheme.") ;; 32-bit systems are not supported by the unpatched code. ;; Following the bug reports https://github.com/amplab/snap/issues/68 and ;; https://bugs.debian.org/cgi-bin/bugreport.cgi?bug=812378 we see that ;; systems without a lot of memory cannot make good use of this program. (supported-systems '("x86_64-linux")) (license license:asl2.0))) (define-public sortmerna (package (name "sortmerna") (version "2.1b") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/biocore/sortmerna") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "0j3mbz4n25738yijmjbr5r4fyvkgm8v5vn3sshyfvmyqf5q9byqf")))) (build-system gnu-build-system) (outputs '("out" ;for binaries "db")) ;for sequence databases (arguments `(#:phases (modify-phases %standard-phases (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (db (assoc-ref outputs "db")) (share (string-append db "/share/sortmerna/rRNA_databases"))) (install-file "sortmerna" bin) (install-file "indexdb_rna" bin) (for-each (lambda (file) (install-file file share)) (find-files "rRNA_databases" ".*fasta")) #t)))))) (inputs `(("zlib" ,zlib))) (home-page "https://bioinfo.lifl.fr/RNA/sortmerna/") (synopsis "Biological sequence analysis tool for NGS reads") (description "SortMeRNA is a biological sequence analysis tool for filtering, mapping and @acronym{OTU, operational taxonomic unit} picking of @acronym{NGS, next generation sequencing} reads. The core algorithm is based on approximate seeds and allows for fast and sensitive analyses of nucleotide sequences. The main application of SortMeRNA is filtering rRNA from metatranscriptomic data.") ;; The source includes x86 specific code (supported-systems '("x86_64-linux" "i686-linux")) (license license:lgpl3))) (define-public star (package (name "star") (version "2.7.8a") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/alexdobin/STAR") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "0zc5biymja9zml9yizcj1h68fq9c6sxfcav8a0lbgvgsm44rvans")) (modules '((guix build utils))) (snippet '(begin (substitute* "source/Makefile" (("/bin/rm") "rm")) ;; Remove pre-built binaries and bundled htslib sources. (delete-file-recursively "bin/MacOSX_x86_64") (delete-file-recursively "bin/Linux_x86_64") (delete-file-recursively "bin/Linux_x86_64_static") (delete-file-recursively "source/htslib") #t)))) (build-system gnu-build-system) (arguments '(#:tests? #f ;no check target #:make-flags '("STAR") #:phases (modify-phases %standard-phases (add-after 'unpack 'enter-source-dir (lambda _ (chdir "source") #t)) (add-after 'enter-source-dir 'make-reproducible (lambda _ (substitute* "Makefile" (("(COMPILATION_TIME_PLACE=\")(.*)(\")" _ pre mid post) (string-append pre "Built with Guix" post))) #t)) ;; See https://github.com/alexdobin/STAR/pull/562 (add-after 'enter-source-dir 'add-missing-header (lambda _ (substitute* "SoloReadFeature_inputRecords.cpp" (("#include \"binarySearch2.h\"" h) (string-append h "\n#include "))) #t)) (add-after 'enter-source-dir 'do-not-use-bundled-htslib (lambda _ (substitute* "Makefile" (("(Depend.list: \\$\\(SOURCES\\) parametersDefault\\.xxd) htslib" _ prefix) prefix)) (substitute* '("BAMfunctions.cpp" "signalFromBAM.h" "bam_cat.h" "bam_cat.c" "STAR.cpp" "bamRemoveDuplicates.cpp") (("#include \"htslib/([^\"]+\\.h)\"" _ header) (string-append "#include <" header ">"))) (substitute* "IncludeDefine.h" (("\"htslib/(htslib/[^\"]+.h)\"" _ header) (string-append "<" header ">"))) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (install-file "STAR" bin)) #t)) (delete 'configure)))) (native-inputs `(("xxd" ,xxd))) (inputs `(("htslib" ,htslib) ("zlib" ,zlib))) (home-page "https://github.com/alexdobin/STAR") (synopsis "Universal RNA-seq aligner") (description "The Spliced Transcripts Alignment to a Reference (STAR) software is based on a previously undescribed RNA-seq alignment algorithm that uses sequential maximum mappable seed search in uncompressed suffix arrays followed by seed clustering and stitching procedure. In addition to unbiased de novo detection of canonical junctions, STAR can discover non-canonical splices and chimeric (fusion) transcripts, and is also capable of mapping full-length RNA sequences.") ;; Only 64-bit systems are supported according to the README. (supported-systems '("x86_64-linux" "mips64el-linux")) ;; STAR is licensed under GPLv3 or later; htslib is MIT-licensed. (license license:gpl3+))) (define-public star-for-pigx (package (inherit star) (name "star") (version "2.7.3a") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/alexdobin/STAR") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1hgiqw5qhs0pc1xazzihcfd92na02xyq2kb469z04y1v51kpvvjq")) (modules '((guix build utils))) (snippet '(begin (substitute* "source/Makefile" (("/bin/rm") "rm")) ;; Remove pre-built binaries and bundled htslib sources. (delete-file-recursively "bin/MacOSX_x86_64") (delete-file-recursively "bin/Linux_x86_64") (delete-file-recursively "bin/Linux_x86_64_static") (delete-file-recursively "source/htslib") #t)))))) (define-public starlong (package (inherit star) (name "starlong") (arguments (substitute-keyword-arguments (package-arguments star) ((#:make-flags flags) `(list "STARlong")) ((#:phases phases) `(modify-phases ,phases ;; Allow extra long sequence reads. (add-after 'unpack 'make-extra-long (lambda _ (substitute* "source/IncludeDefine.h" (("(#define DEF_readNameLengthMax ).*" _ match) (string-append match "900000\n"))) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (install-file "STARlong" bin)) #t)))))))) (define-public subread (package (name "subread") (version "1.6.0") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/subread/subread-" version "/subread-" version "-source.tar.gz")) (sha256 (base32 "0ah0n4jx6ksk2m2j7xk385x2qzmk1y4rfc6a4mfrdqrlq721w99i")))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no "check" target ;; The CC and CCFLAGS variables are set to contain a lot of x86_64 ;; optimizations by default, so we override these flags such that x86_64 ;; flags are only added when the build target is an x86_64 system. #:make-flags (list (let ((system ,(or (%current-target-system) (%current-system))) (flags '("-ggdb" "-fomit-frame-pointer" "-ffast-math" "-funroll-loops" "-fmessage-length=0" "-O9" "-Wall" "-DMAKE_FOR_EXON" "-DMAKE_STANDALONE" "-DSUBREAD_VERSION=\\\"${SUBREAD_VERSION}\\\"")) (flags64 '("-mmmx" "-msse" "-msse2" "-msse3"))) (if (string-prefix? "x86_64" system) (string-append "CCFLAGS=" (string-join (append flags flags64))) (string-append "CCFLAGS=" (string-join flags)))) "-f" "Makefile.Linux" "CC=gcc ${CCFLAGS}") #:phases (modify-phases %standard-phases (add-after 'unpack 'enter-dir (lambda _ (chdir "src") #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (mkdir-p bin) (copy-recursively "../bin" bin)) #t)) ;; no "configure" script (delete 'configure)))) (inputs `(("zlib" ,zlib))) (home-page "http://bioinf.wehi.edu.au/subread-package/") (synopsis "Tool kit for processing next-gen sequencing data") (description "The subread package contains the following tools: subread aligner, a general-purpose read aligner; subjunc aligner: detecting exon-exon junctions and mapping RNA-seq reads; featureCounts: counting mapped reads for genomic features; exactSNP: a SNP caller that discovers SNPs by testing signals against local background noises.") (license license:gpl3+))) (define-public stringtie (package (name "stringtie") (version "1.2.1") (source (origin (method url-fetch) (uri (string-append "http://ccb.jhu.edu/software/stringtie/dl/" "stringtie-" version ".tar.gz")) (sha256 (base32 "1cqllsc1maq4kh92isi8yadgzbmnf042hlnalpk3y59aph1z3bfz")) (modules '((guix build utils))) (snippet '(begin (delete-file-recursively "samtools-0.1.18") #t)))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no test suite #:phases (modify-phases %standard-phases ;; no configure script (delete 'configure) (add-before 'build 'use-system-samtools (lambda _ (substitute* "Makefile" (("stringtie: \\$\\{BAM\\}/libbam\\.a") "stringtie: ")) (substitute* '("gclib/GBam.h" "gclib/GBam.cpp") (("#include \"(bam|sam|kstring).h\"" _ header) (string-append "#include "))) #t)) (add-after 'unpack 'remove-duplicate-typedef (lambda _ ;; This typedef conflicts with the typedef in ;; glibc-2.25/include/bits/types.h (substitute* "gclib/GThreads.h" (("typedef long long __intmax_t;") "")) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (install-file "stringtie" bin) #t)))))) (inputs `(("samtools" ,samtools-0.1) ("zlib" ,zlib))) (home-page "http://ccb.jhu.edu/software/stringtie/") (synopsis "Transcript assembly and quantification for RNA-Seq data") (description "StringTie is a fast and efficient assembler of RNA-Seq sequence alignments into potential transcripts. It uses a novel network flow algorithm as well as an optional de novo assembly step to assemble and quantitate full-length transcripts representing multiple splice variants for each gene locus. Its input can include not only the alignments of raw reads used by other transcript assemblers, but also alignments of longer sequences that have been assembled from those reads. To identify differentially expressed genes between experiments, StringTie's output can be processed either by the Cuffdiff or Ballgown programs.") (license license:artistic2.0))) (define-public taxtastic (package (name "taxtastic") (version "0.8.11") (source (origin ;; The Pypi version does not include tests. (method git-fetch) (uri (git-reference (url "https://github.com/fhcrc/taxtastic") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1sv8mkg64jn7zdwf1jj71c16686yrwxk0apb1l8sjszy9p166g0p")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'prepare-directory (lambda _ ;; The git checkout must be writable for tests. (for-each make-file-writable (find-files ".")) ;; This test fails, but the error is not caught by the test ;; framework, so the tests fail... (substitute* "tests/test_taxit.py" (("self.cmd_fails\\(''\\)") "self.cmd_fails('nothing')")) ;; This version file is expected to be created with git describe. (mkdir-p "taxtastic/data") (with-output-to-file "taxtastic/data/ver" (lambda () (display ,version))) #t)) (add-after 'unpack 'python37-compatibility (lambda _ (substitute* "taxtastic/utils.py" (("import csv") "import csv, errno") (("os.errno") "errno")) #t)) (replace 'check ;; Note, this fails to run with "-v" as it tries to write to a ;; closed output stream. (lambda _ (invoke "python" "-m" "unittest") #t))))) (propagated-inputs `(("python-sqlalchemy" ,python-sqlalchemy) ("python-decorator" ,python-decorator) ("python-biopython" ,python-biopython) ("python-pandas" ,python-pandas) ("python-psycopg2" ,python-psycopg2) ("python-fastalite" ,python-fastalite) ("python-pyyaml" ,python-pyyaml) ("python-six" ,python-six) ("python-jinja2" ,python-jinja2) ("python-dendropy" ,python-dendropy))) (home-page "https://github.com/fhcrc/taxtastic") (synopsis "Tools for taxonomic naming and annotation") (description "Taxtastic is software written in python used to build and maintain reference packages i.e. collections of reference trees, reference alignments, profiles, and associated taxonomic information.") (license license:gpl3+))) (define-public vcftools (package (name "vcftools") (version "0.1.16") (source (origin (method url-fetch) (uri (string-append "https://github.com/vcftools/vcftools/releases/download/v" version "/vcftools-" version ".tar.gz")) (sha256 (base32 "1qqlx7flfv7axrjwkaz6njkscsl1d0jw98ns8d8bh1n1hd1pgz6v")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no "check" target #:make-flags (list "CFLAGS=-O2" ; override "-m64" flag (string-append "PREFIX=" (assoc-ref %outputs "out")) (string-append "MANDIR=" (assoc-ref %outputs "out") "/share/man/man1")))) (native-inputs `(("pkg-config" ,pkg-config))) (inputs `(("perl" ,perl) ("zlib" ,zlib))) (home-page "https://vcftools.github.io/") (synopsis "Tools for working with VCF files") (description "VCFtools is a program package designed for working with VCF files, such as those generated by the 1000 Genomes Project. The aim of VCFtools is to provide easily accessible methods for working with complex genetic variation data in the form of VCF files.") ;; The license is declared as LGPLv3 in the README and ;; at https://vcftools.github.io/license.html (license license:lgpl3))) (define-public infernal (package (name "infernal") (version "1.1.4") (source (origin (method url-fetch) (uri (string-append "http://eddylab.org/software/infernal/" "infernal-" version ".tar.gz")) (sha256 (base32 "1z4mgwqg1j4n5ika08ai8mg9yjyjhf4821jp83v2bgwzxrykqjgr")))) (build-system gnu-build-system) (native-inputs `(("perl" ,perl) ("python" ,python))) ; for tests (home-page "http://eddylab.org/infernal/") (synopsis "Inference of RNA alignments") (description "Infernal (\"INFERence of RNA ALignment\") is a tool for searching DNA sequence databases for RNA structure and sequence similarities. It is an implementation of a special case of profile stochastic context-free grammars called @dfn{covariance models} (CMs). A CM is like a sequence profile, but it scores a combination of sequence consensus and RNA secondary structure consensus, so in many cases, it is more capable of identifying RNA homologs that conserve their secondary structure more than their primary sequence.") ;; Infernal 1.1.3 requires VMX or SSE capability for parallel instructions. (supported-systems '("i686-linux" "x86_64-linux")) (license license:bsd-3))) (define-public r-snapatac (package (name "r-snapatac") (version "2.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/r3fang/SnapATAC") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "037jzlbl436fi7lkpq7d83i2vd1crnrik3vac2x6xj75dbikb2av")))) (properties `((upstream-name . "SnapATAC"))) (build-system r-build-system) (propagated-inputs `(("r-bigmemory" ,r-bigmemory) ("r-doparallel" ,r-doparallel) ("r-dosnow" ,r-dosnow) ("r-edger" ,r-edger) ("r-foreach" ,r-foreach) ("r-genomicranges" ,r-genomicranges) ("r-igraph" ,r-igraph) ("r-iranges" ,r-iranges) ("r-irlba" ,r-irlba) ("r-matrix" ,r-matrix) ("r-plyr" ,r-plyr) ("r-plot3d" ,r-plot3d) ("r-rann" ,r-rann) ("r-raster" ,r-raster) ("r-rcolorbrewer" ,r-rcolorbrewer) ("r-rhdf5" ,r-rhdf5) ("r-rtsne" ,r-rtsne) ("r-scales" ,r-scales) ("r-viridis" ,r-viridis))) (home-page "https://github.com/r3fang/SnapATAC") (synopsis "Single nucleus analysis package for ATAC-Seq") (description "This package provides a fast and accurate analysis toolkit for single cell ATAC-seq (Assay for transposase-accessible chromatin using sequencing). Single cell ATAC-seq can resolve the heterogeneity of a complex tissue and reveal cell-type specific regulatory landscapes. However, the exceeding data sparsity has posed unique challenges for the data analysis. This package @code{r-snapatac} is an end-to-end bioinformatics pipeline for analyzing large- scale single cell ATAC-seq data which includes quality control, normalization, clustering analysis, differential analysis, motif inference and exploration of single cell ATAC-seq sequencing data.") (license license:gpl3))) (define-public r-shinycell (let ((commit "aecbd56e66802f28e397f5ae1f19403aadd12163") (revision "1")) (package (name "r-shinycell") (version (git-version "2.0.0" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/SGDDNB/ShinyCell") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "13jn2ikmvljnzayk485g1mmq5abcp9m1b8n1djdb1agmn83zaki5")))) (properties `((upstream-name . "ShinyCell"))) (build-system r-build-system) (propagated-inputs `(("r-data-table" ,r-data-table) ("r-ggplot2" ,r-ggplot2) ("r-glue" ,r-glue) ("r-gridextra" ,r-gridextra) ("r-hdf5r" ,r-hdf5r) ("r-matrix" ,r-matrix) ("r-r-utils" ,r-r-utils) ("r-rcolorbrewer" ,r-rcolorbrewer) ("r-readr" ,r-readr) ("r-reticulate" ,r-reticulate))) (home-page "https://github.com/SGDDNB/ShinyCell") (synopsis "Shiny interactive web apps for single-cell data") (description "This package provides Shiny apps for interactive exploration of single-cell data.") (license license:gpl3)))) (define-public r-archr (let ((commit "46b519ffb6f73edf132497ac31650d19ef055dc1") (revision "1")) (package (name "r-archr") (version (git-version "1.0.0" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/GreenleafLab/ArchR") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "1zj3sdfhgn2q2256fmz61a92vw1wylyck632d7842d6knd0v92v8")))) (properties `((upstream-name . "ArchR"))) (build-system r-build-system) (propagated-inputs `(("r-biocgenerics" ,r-biocgenerics) ("r-biostrings" ,r-biostrings) ("r-chromvar" ,r-chromvar) ("r-complexheatmap" ,r-complexheatmap) ("r-data-table" ,r-data-table) ("r-genomicranges" ,r-genomicranges) ("r-ggplot2" ,r-ggplot2) ("r-ggrepel" ,r-ggrepel) ("r-gridextra" ,r-gridextra) ("r-gtable" ,r-gtable) ("r-gtools" ,r-gtools) ("r-magrittr" ,r-magrittr) ("r-matrix" ,r-matrix) ("r-matrixstats" ,r-matrixstats) ("r-motifmatchr" ,r-motifmatchr) ("r-nabor" ,r-nabor) ("r-plyr" ,r-plyr) ("r-rcpp" ,r-rcpp) ("r-rhdf5" ,r-rhdf5) ("r-rsamtools" ,r-rsamtools) ("r-s4vectors" ,r-s4vectors) ("r-stringr" ,r-stringr) ("r-summarizedexperiment" ,r-summarizedexperiment) ("r-uwot" ,r-uwot))) (home-page "https://github.com/GreenleafLab/ArchR") (synopsis "Analyze single-cell regulatory chromatin in R") (description "This package is designed to streamline scATAC analyses in R.") (license license:gpl2+)))) (define-public r-scde (package (name "r-scde") (version "1.99.2") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/hms-dbmi/scde") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "10na2gyka24mszdxf92wz9h2c13hdf1ww30c68gfsw53lvvhhhxb")))) (build-system r-build-system) (propagated-inputs `(("r-rcpp" ,r-rcpp) ("r-rcpparmadillo" ,r-rcpparmadillo) ("r-mgcv" ,r-mgcv) ("r-rook" ,r-rook) ("r-rjson" ,r-rjson) ("r-cairo" ,r-cairo) ("r-rcolorbrewer" ,r-rcolorbrewer) ("r-edger" ,r-edger) ("r-quantreg" ,r-quantreg) ("r-nnet" ,r-nnet) ("r-rmtstat" ,r-rmtstat) ("r-extremes" ,r-extremes) ("r-pcamethods" ,r-pcamethods) ("r-biocparallel" ,r-biocparallel) ("r-flexmix" ,r-flexmix))) (home-page "https://hms-dbmi.github.io/scde/") (synopsis "R package for analyzing single-cell RNA-seq data") (description "The SCDE package implements a set of statistical methods for analyzing single-cell RNA-seq data. SCDE fits individual error models for single-cell RNA-seq measurements. These models can then be used for assessment of differential expression between groups of cells, as well as other types of analysis. The SCDE package also contains the pagoda framework which applies pathway and gene set overdispersion analysis to identify aspects of transcriptional heterogeneity among single cells.") ;; See https://github.com/hms-dbmi/scde/issues/38 (license license:gpl2))) (define-public r-centipede (package (name "r-centipede") (version "1.2") (source (origin (method url-fetch) (uri (string-append "http://download.r-forge.r-project.org/" "src/contrib/CENTIPEDE_" version ".tar.gz")) (sha256 (base32 "1hsx6qgwr0i67fhy9257zj7s0ppncph2hjgbia5nn6nfmj0ax6l9")))) (build-system r-build-system) (home-page "http://centipede.uchicago.edu/") (synopsis "Predict transcription factor binding sites") (description "CENTIPEDE applies a hierarchical Bayesian mixture model to infer regions of the genome that are bound by particular transcription factors. It starts by identifying a set of candidate binding sites, and then aims to classify the sites according to whether each site is bound or not bound by a transcription factor. CENTIPEDE is an unsupervised learning algorithm that discriminates between two different types of motif instances using as much relevant information as possible.") (license (list license:gpl2+ license:gpl3+)))) (define-public r-demultiplex (let ((commit "6e2a1422c8e6f418cfb271997eebc91f9195f299") (revision "1")) (package (name "r-demultiplex") (version (git-version "1.0.2" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/chris-mcginnis-ucsf/MULTI-seq") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "01kv88wp8vdaq07sjk0d3d1cb553mq1xqg0war81pgmg63bgi38w")))) (properties `((upstream-name . "deMULTIplex"))) (build-system r-build-system) (propagated-inputs `(("r-kernsmooth" ,r-kernsmooth) ("r-reshape2" ,r-reshape2) ("r-rtsne" ,r-rtsne) ("r-shortread" ,r-shortread) ("r-stringdist" ,r-stringdist))) (home-page "https://github.com/chris-mcginnis-ucsf/MULTI-seq") (synopsis "MULTI-seq pre-processing and classification tools") (description "deMULTIplex is an R package for analyzing single-cell RNA sequencing data generated with the MULTI-seq sample multiplexing method. The package includes software to @enumerate @item Convert raw MULTI-seq sample barcode library FASTQs into a sample barcode UMI count matrix, and @item Classify cell barcodes into sample barcode groups. @end enumerate ") (license license:cc0)))) (define-public vsearch (package (name "vsearch") (version "2.9.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/torognes/vsearch") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0vhrpjfdf75ba04b24xknp41790cvcgwl0vgpy7qbzj5xh2521ss")) (patches (search-patches "vsearch-unbundle-cityhash.patch")) (snippet '(begin ;; Remove bundled cityhash sources. The vsearch source is adjusted ;; for this in the patch. (delete-file "src/city.h") (delete-file "src/citycrc.h") (delete-file "src/city.cc") #t)))) (build-system gnu-build-system) (inputs `(("zlib" ,zlib) ("bzip2" ,bzip2) ("cityhash" ,cityhash))) (native-inputs `(("autoconf" ,autoconf) ("automake" ,automake))) (synopsis "Sequence search tools for metagenomics") (description "VSEARCH supports DNA sequence searching, clustering, chimera detection, dereplication, pairwise alignment, shuffling, subsampling, sorting and masking. The tool takes advantage of parallelism in the form of SIMD vectorization as well as multiple threads to perform accurate alignments at high speed. VSEARCH uses an optimal global aligner (full dynamic programming Needleman-Wunsch).") (home-page "https://github.com/torognes/vsearch") ;; vsearch uses non-portable SSE intrinsics so building fails on other ;; platforms. (supported-systems '("x86_64-linux")) ;; Dual licensed; also includes public domain source. (license (list license:gpl3 license:bsd-2)))) (define-public pardre (package (name "pardre") ;; The source of 1.1.5 changed in place, so we append "-1" to the version. (version "1.1.5-1") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/pardre/ParDRe-rel" "1.1.5" ".tar.gz")) (sha256 (base32 "17j73nc0viq4f6qj50nrndsrif5d6b71q8fl87m54psiv0ilns2b")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no tests included #:phases (modify-phases %standard-phases (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (install-file "ParDRe" bin) #t)))))) (inputs `(("openmpi" ,openmpi) ("zlib" ,zlib))) (synopsis "Parallel tool to remove duplicate DNA reads") (description "ParDRe is a parallel tool to remove duplicate genetic sequence reads. Duplicate reads can be seen as identical or nearly identical sequences with some mismatches. This tool lets users avoid the analysis of unnecessary reads, reducing the time of subsequent procedures with the dataset (e.g. assemblies, mappings, etc.). The tool is implemented with MPI in order to exploit the parallel capabilities of multicore clusters. It is faster than multithreaded counterparts (end of 2015) for the same number of cores and, thanks to the message-passing technology, it can be executed on clusters.") (home-page "https://sourceforge.net/projects/pardre/") (license license:gpl3+))) (define-public ngshmmalign (package (name "ngshmmalign") (version "0.1.1") (source (origin (method url-fetch) (uri (string-append "https://github.com/cbg-ethz/ngshmmalign/" "releases/download/" version "/ngshmmalign-" version ".tar.bz2")) (sha256 (base32 "0jryvlssi2r2ii1dxnx39yk6bh4yqgq010fnxrgfgbaj3ykijlzv")))) (build-system cmake-build-system) (arguments '(#:tests? #false)) ; there are none (inputs `(("boost" ,boost))) (home-page "https://github.com/cbg-ethz/ngshmmalign/") (synopsis "Profile HMM aligner for NGS reads") (description "ngshmmalign is a profile HMM aligner for NGS reads designed particularly for small genomes (such as those of RNA viruses like HIV-1 and HCV) that experience substantial biological insertions and deletions.") (license license:gpl2+))) (define-public prinseq (package (name "prinseq") (version "0.20.4") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/prinseq/standalone/" "prinseq-lite-" version ".tar.gz")) (sha256 (base32 "0vxmzvmm67whxrqdaaamwgjk7cf0fzfs5s673jgg00kz7g70splv")))) (build-system gnu-build-system) (arguments `(#:tests? #false ; no check target #:phases (modify-phases %standard-phases (delete 'configure) (delete 'build) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (scripts (find-files "." "prinseq.*.pl"))) (substitute* scripts (("\"perl -pe") (string-append "\"" (which "perl") " -pe"))) (for-each (lambda (file) (chmod file #o555) (install-file file bin) (wrap-script (string-append bin "/" (basename file)) `("PERL5LIB" ":" prefix (,(getenv "PERL5LIB"))))) scripts))))))) (inputs `(("guile" ,guile-3.0) ; for wrapper scripts ("perl" ,perl) ("perl-cairo" ,perl-cairo) ("perl-data-dumper" ,perl-data-dumper) ("perl-digest-md5" ,perl-digest-md5) ("perl-getopt-long" ,perl-getopt-long) ("perl-json" ,perl-json) ("perl-statistics-pca" ,perl-statistics-pca))) (home-page "http://prinseq.sourceforge.net/") (synopsis "Preprocess sequence data in FASTA or FASTQ formats") (description "PRINSEQ is a bioinformatics tool to help you preprocess your genomic or metagenomic sequence data in FASTA or FASTQ formats. The tool is written in Perl and can be helpful if you want to filter, reformat, or trim your sequence data. It also generates basic statistics for your sequences.") (license license:gpl3+))) (define-public shorah (package (name "shorah") (version "1.99.2") (source (origin (method url-fetch) (uri (string-append "https://github.com/cbg-ethz/shorah" "/releases/download/v" version "/shorah-" version ".tar.xz")) (sha256 (base32 "158dir9qcqspknlnyfr9zwk41x48nrh5wcg10k2grh9cidp9daiq")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'fix-test-wrapper (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (substitute* "examples/run_end2end_test" (("\\$\\{interpreter\\} ../\\$\\{testscript\\}") (string-append bin "/${testscript}")))))) (delete 'check) (add-after 'install 'wrap-programs (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (site (string-append out "/lib/python" ,(version-major+minor (package-version python)) "/site-packages")) (pythonpath (getenv "PYTHONPATH")) (script (string-append out "/bin/shorah"))) (chmod script #o555) (wrap-program script `("PYTHONPATH" ":" prefix (,site ,pythonpath)))))) (add-after 'wrap-programs 'check (lambda* (#:key tests? #:allow-other-keys) (when tests? (invoke "make" "check"))))))) (inputs `(("boost" ,boost) ("htslib" ,htslib) ("python" ,python) ("python-biopython" ,python-biopython) ("python-numpy" ,python-numpy) ("zlib" ,zlib))) (native-inputs `(("pkg-config" ,pkg-config))) (home-page "") (synopsis "Short reads assembly into haplotypes") (description "ShoRAH is a project for the analysis of next generation sequencing data. It is designed to analyse genetically heterogeneous samples. Its tools provide error correction, haplotype reconstruction and estimation of the frequency of the different genetic variants present in a mixed sample.") (license license:gpl3+))) (define-public ruby-bio-kseq (package (name "ruby-bio-kseq") (version "0.0.2") (source (origin (method url-fetch) (uri (rubygems-uri "bio-kseq" version)) (sha256 (base32 "1xyaha46khb5jc6wzkbf7040jagac49jbimn0vcrzid0j8jdikrz")))) (build-system ruby-build-system) (arguments `(#:test-target "spec")) (native-inputs `(("bundler" ,bundler) ("ruby-rspec" ,ruby-rspec) ("ruby-rake-compiler" ,ruby-rake-compiler))) (inputs `(("zlib" ,zlib))) (synopsis "Ruby bindings for the kseq.h FASTA/Q parser") (description "@code{Bio::Kseq} provides ruby bindings to the @code{kseq.h} FASTA and FASTQ parsing code. It provides a fast iterator over sequences and their quality scores.") (home-page "https://github.com/gusevfe/bio-kseq") (license license:expat))) (define-public bio-locus (package (name "bio-locus") (version "0.0.7") (source (origin (method url-fetch) (uri (rubygems-uri "bio-locus" version)) (sha256 (base32 "02vmrxyimkj9sahsp4zhfhnmbvz6dbbqz1y01vglf8cbwvkajfl0")))) (build-system ruby-build-system) (native-inputs `(("ruby-rspec" ,ruby-rspec))) (synopsis "Tool for fast querying of genome locations") (description "Bio-locus is a tabix-like tool for fast querying of genome locations. Many file formats in bioinformatics contain records that start with a chromosome name and a position for a SNP, or a start-end position for indels. Bio-locus allows users to store this chr+pos or chr+pos+alt information in a database.") (home-page "https://github.com/pjotrp/bio-locus") (license license:expat))) (define-public bio-blastxmlparser (package (name "bio-blastxmlparser") (version "2.0.4") (source (origin (method url-fetch) (uri (rubygems-uri "bio-blastxmlparser" version)) (sha256 (base32 "1wf4qygcmdjgcqm6flmvsagfr1gs9lf63mj32qv3z1f481zc5692")))) (build-system ruby-build-system) (propagated-inputs `(("ruby-bio-logger" ,ruby-bio-logger) ("ruby-nokogiri" ,ruby-nokogiri))) (inputs `(("ruby-rspec" ,ruby-rspec))) (synopsis "Fast big data BLAST XML parser and library") (description "Very fast parallel big-data BLAST XML file parser which can be used as command line utility. Use blastxmlparser to: Parse BLAST XML; filter output; generate FASTA, JSON, YAML, RDF, JSON-LD, HTML, CSV, tabular output etc.") (home-page "https://github.com/pjotrp/blastxmlparser") (license license:expat))) (define-public bioruby (package (name "bioruby") (version "1.5.2") (source (origin (method url-fetch) (uri (rubygems-uri "bio" version)) (sha256 (base32 "1d56amdsjv1mag7m6gv2w0xij8hqx1v5xbdjsix8sp3yp36m7938")))) (build-system ruby-build-system) (propagated-inputs `(("ruby-libxml" ,ruby-libxml))) (native-inputs `(("which" ,which))) ; required for test phase (arguments `(#:phases (modify-phases %standard-phases (add-before 'build 'patch-test-command (lambda _ (substitute* '("test/functional/bio/test_command.rb") (("/bin/sh") (which "sh"))) (substitute* '("test/functional/bio/test_command.rb") (("/bin/ls") (which "ls"))) (substitute* '("test/functional/bio/test_command.rb") (("which") (which "which"))) (substitute* '("test/functional/bio/test_command.rb", "test/data/command/echoarg2.sh") (("/bin/echo") (which "echo"))) #t))))) (synopsis "Ruby library, shell and utilities for bioinformatics") (description "BioRuby comes with a comprehensive set of Ruby development tools and libraries for bioinformatics and molecular biology. BioRuby has components for sequence analysis, pathway analysis, protein modelling and phylogenetic analysis; it supports many widely used data formats and provides easy access to databases, external programs and public web services, including BLAST, KEGG, GenBank, MEDLINE and GO.") (home-page "http://bioruby.org/") ;; Code is released under Ruby license, except for setup ;; (LGPLv2.1+) and scripts in samples (which have GPL2 and GPL2+) (license (list license:ruby license:lgpl2.1+ license:gpl2+ )))) (define-public bio-vcf (package (name "bio-vcf") (version "0.9.5") (source (origin (method url-fetch) (uri (rubygems-uri "bio-vcf" version)) (sha256 (base32 "1glw5pn9s8z13spxk6yyfqaz80n9lga67f33w35nkpq9dwi2vg6g")))) (build-system ruby-build-system) (native-inputs `(("ruby-cucumber" ,ruby-cucumber))) (synopsis "Smart VCF parser DSL") (description "Bio-vcf provides a @acronym{DSL, domain specific language} for processing the VCF format. Record named fields can be queried with regular expressions. Bio-vcf is a new generation VCF parser, filter and converter. Bio-vcf is not only very fast for genome-wide (WGS) data, it also comes with a filtering, evaluation and rewrite language and can output any type of textual data, including VCF header and contents in RDF and JSON.") (home-page "https://github.com/vcflib/bio-vcf") (license license:expat))) (define-public r-phantompeakqualtools (let ((commit "8d2b2d18c686d894ef5908b37da7adf72a07ef42") (revision "1")) (package (name "r-phantompeakqualtools") (version (git-version "1.2.2" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/kundajelab/phantompeakqualtools") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "00anrvcwsp02d98qhj1xpj85644h2pp4kfzq6dgbmwmdr6jvy7p4")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; There are no tests. #:phases (modify-phases %standard-phases (delete 'configure) (delete 'build) (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let ((script (string-append (assoc-ref outputs "out") "/share/scripts"))) (install-file "run_spp.R" script))))))) (inputs `(("r" ,r-minimal))) (propagated-inputs `(("r-catools" ,r-catools) ("r-snow" ,r-snow) ("r-snowfall" ,r-snowfall) ("r-bitops" ,r-bitops) ("r-rsamtools" ,r-rsamtools) ("r-spp" ,r-spp) ("gawk" ,gawk) ("samtools" ,samtools) ("boost" ,boost) ("gzip" ,gzip))) (home-page "https://github.com/kundajelab/phantompeakqualtools") (synopsis "Informative enrichment for ChIP-seq data") (description "This package computes informative enrichment and quality measures for ChIP-seq/DNase-seq/FAIRE-seq/MNase-seq data. It can also be used to obtain robust estimates of the predominant fragment length or characteristic tag shift values in these assays.") (license license:bsd-3)))) (define-public r-r4rna (package (name "r-r4rna") (version "0.1.4") (source (origin (method url-fetch) (uri (string-append "http://www.e-rna.org/r-chie/files/R4RNA_" version ".tar.gz")) (sha256 (base32 "1p0i78wh76jfgmn9jphbwwaz6yy6pipzfg08xs54cxavxg2j81p5")))) (build-system r-build-system) (propagated-inputs `(("r-optparse" ,r-optparse) ("r-rcolorbrewer" ,r-rcolorbrewer))) (home-page "https://www.e-rna.org/r-chie/index.cgi") (synopsis "Analysis framework for RNA secondary structure") (description "The R4RNA package aims to be a general framework for the analysis of RNA secondary structure and comparative analysis in R.") (license license:gpl3+))) (define-public rcas-web (package (name "rcas-web") (version "0.1.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/BIMSBbioinfo/rcas-web/" "releases/download/v" version "/rcas-web-" version ".tar.gz")) (sha256 (base32 "0wq951aj45gqki1bickg876i993lmawkp8x24agg264br5x716db")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-before 'configure 'find-RCAS ;; The configure script can't find non-1.3.x versions of RCAS because ;; its R expression ‘1.10.1 >= 1.3.4’ evaluates to false. (lambda _ (substitute* "configure" (("1\\.3\\.4") "0.0.0")) #t)) (add-after 'install 'wrap-executable (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (json (assoc-ref inputs "guile-json")) (redis (assoc-ref inputs "guile-redis")) (path (string-append json "/share/guile/site/2.2:" redis "/share/guile/site/2.2"))) (wrap-program (string-append out "/bin/rcas-web") `("GUILE_LOAD_PATH" ":" = (,path)) `("GUILE_LOAD_COMPILED_PATH" ":" = (,path)) `("R_LIBS_SITE" ":" = (,(getenv "R_LIBS_SITE"))))) #t))))) (inputs `(("r-minimal" ,r-minimal) ("r-rcas" ,r-rcas) ("guile" ,guile-2.2) ("guile-json" ,guile-json-1) ("guile-redis" ,guile2.2-redis))) (native-inputs `(("pkg-config" ,pkg-config))) (home-page "https://github.com/BIMSBbioinfo/rcas-web") (synopsis "Web interface for RNA-centric annotation system (RCAS)") (description "This package provides a simple web interface for the @dfn{RNA-centric annotation system} (RCAS).") (license license:agpl3+))) (define-public r-chipkernels (let ((commit "c9cfcacb626b1221094fb3490ea7bac0fd625372") (revision "1")) (package (name "r-chipkernels") (version (git-version "1.1" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ManuSetty/ChIPKernels") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "14bj5qhjm1hsm9ay561nfbqi9wxsa7y487df2idsaaf6z10nw4v0")))) (build-system r-build-system) (propagated-inputs `(("r-iranges" ,r-iranges) ("r-xvector" ,r-xvector) ("r-biostrings" ,r-biostrings) ("r-bsgenome" ,r-bsgenome) ("r-gtools" ,r-gtools) ("r-genomicranges" ,r-genomicranges) ("r-sfsmisc" ,r-sfsmisc) ("r-kernlab" ,r-kernlab) ("r-s4vectors" ,r-s4vectors) ("r-biocgenerics" ,r-biocgenerics))) (home-page "https://github.com/ManuSetty/ChIPKernels") (synopsis "Build string kernels for DNA Sequence analysis") (description "ChIPKernels is an R package for building different string kernels used for DNA Sequence analysis. A dictionary of the desired kernel must be built and this dictionary can be used for determining kernels for DNA Sequences.") (license license:gpl2+)))) (define-public r-seqgl (package (name "r-seqgl") (version "1.1.4") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ManuSetty/SeqGL") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1r6ywvhxl3ffv48lgj7sbd582mcc6dha3ksgc2qjlvjrnkbj3799")))) (build-system r-build-system) (propagated-inputs `(("r-biostrings" ,r-biostrings) ("r-chipkernels" ,r-chipkernels) ("r-genomicranges" ,r-genomicranges) ("r-spams" ,r-spams) ("r-wgcna" ,r-wgcna) ("r-fastcluster" ,r-fastcluster))) (home-page "https://github.com/ManuSetty/SeqGL") (synopsis "Group lasso for Dnase/ChIP-seq data") (description "SeqGL is a group lasso based algorithm to extract transcription factor sequence signals from ChIP, DNase and ATAC-seq profiles. This package presents a method which uses group lasso to discriminate between bound and non bound genomic regions to accurately identify transcription factors bound at the specific regions.") (license license:gpl2+))) (define-public emboss (package (name "emboss") (version "6.5.7") (source (origin (method url-fetch) (uri (string-append "ftp://emboss.open-bio.org/pub/EMBOSS/old/" (version-major+minor version) ".0/" "EMBOSS-" version ".tar.gz")) (sha256 (base32 "0vsmz96gc411yj2iyzdrsmg4l2n1nhgmp7vrgzlxx3xixv9xbf0q")))) (build-system gnu-build-system) (arguments `(#:configure-flags (list (string-append "--with-hpdf=" (assoc-ref %build-inputs "libharu"))) #:phases (modify-phases %standard-phases (add-after 'unpack 'fix-checks (lambda _ ;; The PNGDRIVER tests check for the presence of libgd, libpng ;; and zlib, but assume that they are all found at the same ;; prefix. (substitute* "configure.in" (("CHECK_PNGDRIVER") "LIBS=\"$LIBS -lgd -lpng -lz -lm\" AC_DEFINE([PLD_png], [1], [Define to 1 if PNG support is available]) AM_CONDITIONAL(AMPNG, true)")) #t)) (add-after 'fix-checks 'disable-update-check (lambda _ ;; At build time there is no connection to the Internet, so ;; looking for updates will not work. (substitute* "Makefile.am" (("\\$\\(bindir\\)/embossupdate") "")) #t)) (add-after 'disable-update-check 'autogen (lambda _ (invoke "autoreconf" "-vif") #t))))) (inputs `(("perl" ,perl) ("libpng" ,libpng) ("gd" ,gd) ("libx11" ,libx11) ("libharu" ,libharu) ("zlib" ,zlib))) (native-inputs `(("autoconf" ,autoconf) ("automake" ,automake) ("libtool" ,libtool) ("pkg-config" ,pkg-config))) (home-page "http://emboss.sourceforge.net") (synopsis "Molecular biology analysis suite") (description "EMBOSS is the \"European Molecular Biology Open Software Suite\". EMBOSS is an analysis package specially developed for the needs of the molecular biology (e.g. EMBnet) user community. The software automatically copes with data in a variety of formats and even allows transparent retrieval of sequence data from the web. It also provides a number of libraries for the development of software in the field of molecular biology. EMBOSS also integrates a range of currently available packages and tools for sequence analysis into a seamless whole.") (license license:gpl2+))) (define-public bits (let ((revision "1") (commit "3cc4567896d9d6442923da944beb704750a08d2d")) (package (name "bits") ;; The version is 2.13.0 even though no release archives have been ;; published as yet. (version (git-version "2.13.0" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/arq5x/bits") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "17n2kffk4kmhivd8c98g2vr6y1s23vbg4sxlxs689wni66797hbs")))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no tests included #:phases (modify-phases %standard-phases (delete 'configure) (add-after 'unpack 'remove-cuda (lambda _ (substitute* "Makefile" ((".*_cuda") "") (("(bits_test_intersections) \\\\" _ match) match)) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (copy-recursively "bin" (string-append (assoc-ref outputs "out") "/bin")) #t))))) (inputs `(("gsl" ,gsl) ("zlib" ,zlib))) (home-page "https://github.com/arq5x/bits") (synopsis "Implementation of binary interval search algorithm") (description "This package provides an implementation of the BITS (Binary Interval Search) algorithm, an approach to interval set intersection. It is especially suited for the comparison of diverse genomic datasets and the exploration of large datasets of genome intervals (e.g. genes, sequence alignments).") (license license:gpl2)))) (define-public piranha ;; There is no release tarball for the latest version. The latest commit is ;; older than one year at the time of this writing. (let ((revision "1") (commit "0466d364b71117d01e4471b74c514436cc281233")) (package (name "piranha") (version (git-version "1.2.1" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/smithlabcode/piranha") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "117dc0zf20c61jam69sk4abl57ah6yi6i7qra7d7y5zrbgk12q5n")))) (build-system gnu-build-system) (arguments `(#:test-target "test" #:phases (modify-phases %standard-phases (add-after 'unpack 'copy-smithlab-cpp (lambda* (#:key inputs #:allow-other-keys) (for-each (lambda (file) (install-file file "./src/smithlab_cpp/")) (find-files (assoc-ref inputs "smithlab-cpp"))) #t)) (add-after 'install 'install-to-store (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin"))) (for-each (lambda (file) (install-file file bin)) (find-files "bin" ".*"))) #t))) #:configure-flags (list (string-append "--with-bam_tools_headers=" (assoc-ref %build-inputs "bamtools") "/include/bamtools") (string-append "--with-bam_tools_library=" (assoc-ref %build-inputs "bamtools") "/lib/bamtools")))) (inputs `(("bamtools" ,bamtools) ("samtools" ,samtools-0.1) ("gsl" ,gsl) ("smithlab-cpp" ,(let ((commit "3723e2db438c51501d0423429ff396c3035ba46a")) (origin (method git-fetch) (uri (git-reference (url "https://github.com/smithlabcode/smithlab_cpp") (commit commit))) (file-name (string-append "smithlab_cpp-" commit "-checkout")) (sha256 (base32 "0l4gvbwslw5ngziskja41c00x1r06l3yidv7y0xw9djibhykzy0g"))))))) (native-inputs `(("python" ,python-2))) (home-page "https://github.com/smithlabcode/piranha") (synopsis "Peak-caller for CLIP-seq and RIP-seq data") (description "Piranha is a peak-caller for genomic data produced by CLIP-seq and RIP-seq experiments. It takes input in BED or BAM format and identifies regions of statistically significant read enrichment. Additional covariates may optionally be provided to further inform the peak-calling process.") (license license:gpl3+)))) (define-public pepr (package (name "pepr") (version "1.0.9") (source (origin (method url-fetch) (uri (pypi-uri "PePr" version)) (sha256 (base32 "0qxjfdpl1b1y53nccws2d85f6k74zwmx8y8sd9rszcqhfayx6gdx")))) (build-system python-build-system) (arguments `(#:python ,python-2 ; python2 only #:tests? #f)) ; no tests included (propagated-inputs `(("python2-numpy" ,python2-numpy) ("python2-scipy" ,python2-scipy) ("python2-pysam" ,python2-pysam))) (home-page "https://github.com/shawnzhangyx/PePr") (synopsis "Peak-calling and prioritization pipeline for ChIP-Seq data") (description "PePr is a ChIP-Seq peak calling or differential binding analysis tool that is primarily designed for data with biological replicates. It uses a negative binomial distribution to model the read counts among the samples in the same group, and look for consistent differences between ChIP and control group or two ChIP groups run under different conditions.") (license license:gpl3+))) (define-public filevercmp (let ((commit "1a9b779b93d0b244040274794d402106907b71b7") (revision "1")) (package (name "filevercmp") (version (git-version "0" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ekg/filevercmp") (commit commit))) (file-name (git-file-name name commit)) (sha256 (base32 "1j9vxsy0y050v59h0q1d6501fcw1kjvj0d18l1xk2zyg0jzj247c")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; There are no tests to run. #:phases (modify-phases %standard-phases (delete 'configure) ; There is no configure phase. (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) (install-file "filevercmp" (string-append out "/bin")) (install-file "filevercmp.h" (string-append out "/include")) #t)))))) (home-page "https://github.com/ekg/filevercmp") (synopsis "This program compares version strings") (description "This program compares version strings. It intends to be a replacement for strverscmp.") (license license:gpl3+)))) (define-public multiqc (package (name "multiqc") (version "1.10.1") (source (origin (method url-fetch) (uri (pypi-uri "multiqc" version)) (sha256 (base32 "0y9sgjca3bp0kk3ngry4zf4q2diyzp5bvzsx5l23nsysfbfkigm4")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (replace 'check (lambda* (#:key inputs outputs tests? #:allow-other-keys) (when tests? (setenv "HOME" "/tmp") (let ((here (getcwd))) (copy-recursively (assoc-ref inputs "tests") "/tmp/tests") ;; ModuleNotFoundError: No module named 'multiqc.modules.ccs' (delete-file "/tmp/tests/unit_tests/test_ccs.py") (with-directory-excursion "/tmp/tests" (setenv "PYTHONPATH" (string-append here ":" (getenv "PYTHONPATH"))) (invoke "python" "-munittest" "discover"))))))))) (propagated-inputs `(("python-click" ,python-click) ("python-coloredlogs" ,python-coloredlogs) ("python-future" ,python-future) ("python-jinja2" ,python-jinja2) ("python-lzstring" ,python-lzstring) ("python-markdown" ,python-markdown) ("python-matplotlib" ,python-matplotlib) ("python-networkx" ,python-networkx) ("python-numpy" ,python-numpy) ("python-pyyaml" ,python-pyyaml) ("python-requests" ,python-requests) ("python-rich" ,python-rich) ("python-simplejson" ,python-simplejson) ("python-spectra" ,python-spectra))) (native-inputs `(("python-pytest" ,python-pytest) ("tests" ,(let ((commit "02272d48a382beb27489fcf9e6308a0407dc3c2e")) (origin (method git-fetch) (uri (git-reference (url "https://github.com/ewels/MultiQC_TestData") (commit commit))) (file-name (git-file-name "multiqc-test-data" commit)) (sha256 (base32 "1bha64wanrigczw4yn81din56396n61j5gqdrkslhslmskcafi91"))))))) (home-page "https://multiqc.info") (synopsis "Aggregate bioinformatics analysis reports") (description "MultiQC is a tool to aggregate bioinformatics results across many samples into a single report. It contains modules for a large number of common bioinformatics tools.") (license license:gpl3+))) (define-public variant-tools (package (name "variant-tools") (version "3.1.2") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/vatlab/varianttools") ;; There is no tag corresponding to version 3.1.2 (commit "813ae4a90d25b69abc8a40f4f70441fe09015249"))) (file-name (git-file-name name version)) (sha256 (base32 "12ibdmksj7icyqhks4xyvd61bygk4pjmxn618kp6vgk1af01y34g")))) (build-system python-build-system) (inputs `(("boost" ,boost) ("c-blosc" ,c-blosc) ("gsl" ,gsl) ("hdf5" ,hdf5) ("hdf5-blosc" ,hdf5-blosc) ("python-cython" ,python-cython) ("zlib" ,zlib))) (propagated-inputs `(("python-numpy" ,python-numpy) ("python-pycurl" ,python-pycurl) ("python-pyzmq" ,python-pyzmq) ("python-scipy" ,python-scipy) ("python-tables" ,python-tables))) (home-page "https://vatlab.github.io/vat-docs/") (synopsis "Analyze genetic variants from Next-Gen sequencing studies") (description "Variant tools is a tool for the manipulation, annotation, selection, simulation, and analysis of variants in the context of next-gen sequencing analysis. Unlike some other tools used for next-gen sequencing analysis, variant tools is project based and provides a whole set of tools to manipulate and analyze genetic variants.") (license license:gpl3+))) (define-public r-raremetals2 (package (name "r-raremetals2") (version "0.1") (source (origin (method url-fetch) (uri (string-append "http://genome.sph.umich.edu/w/images/" "b/b7/RareMETALS2_" version ".tar.gz")) (sha256 (base32 "0z5ljcgvnm06ja9lm85a3cniq7slxcy37aqqkxrdidr79an5fs4s")))) (properties `((upstream-name . "RareMETALS2"))) (build-system r-build-system) (propagated-inputs `(("r-seqminer" ,r-seqminer) ("r-mvtnorm" ,r-mvtnorm) ("r-mass" ,r-mass) ("r-compquadform" ,r-compquadform) ("r-getopt" ,r-getopt))) (home-page "http://genome.sph.umich.edu/wiki/RareMETALS2") (synopsis "Analyze gene-level association tests for binary trait") (description "The R package rareMETALS2 is an extension of the R package rareMETALS. It was designed to meta-analyze gene-level association tests for binary trait. While rareMETALS offers a near-complete solution for meta-analysis of gene-level tests for quantitative trait, it does not offer the optimal solution for binary trait. The package rareMETALS2 offers improved features for analyzing gene-level association tests in meta-analyses for binary trait.") (license license:gpl3))) (define-public r-dropbead (let ((commit "d746c6f3b32110428ea56d6a0001ce52a251c247") (revision "2")) (package (name "r-dropbead") (version (string-append "0-" revision "." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/rajewsky-lab/dropbead") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "0sbzma49aiiyw8b0jpr7fnhzys9nsqmp4hy4hdz1gzyg1lhnca26")))) (build-system r-build-system) (propagated-inputs `(("r-ggplot2" ,r-ggplot2) ("r-rcolorbrewer" ,r-rcolorbrewer) ("r-gridextra" ,r-gridextra) ("r-gplots" ,r-gplots) ("r-plyr" ,r-plyr))) (home-page "https://github.com/rajewsky-lab/dropbead") (synopsis "Basic exploration and analysis of Drop-seq data") (description "This package offers a quick and straight-forward way to explore and perform basic analysis of single cell sequencing data coming from droplet sequencing. It has been particularly tailored for Drop-seq.") (license license:gpl3)))) (define-public r-cellchat (let ((commit "21edd226ca408e4c413408f98562d71ee0b54e5d") (revision "1")) (package (name "r-cellchat") (version (git-version "1.0.0" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/sqjin/CellChat") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "0cvzl9mi8jjznpql2gv67swnk1dndn3a2h22z5l84h7lwpwjmh53")) (snippet '(for-each delete-file '("src/CellChat.so" "src/CellChat_Rcpp.o" "src/RcppExports.o"))))) (properties `((upstream-name . "CellChat"))) (build-system r-build-system) (propagated-inputs `(("r-biocgenerics" ,r-biocgenerics) ("r-circlize" ,r-circlize) ("r-colorspace" ,r-colorspace) ("r-complexheatmap" ,r-complexheatmap) ("r-cowplot" ,r-cowplot) ("r-dplyr" ,r-dplyr) ("r-expm" ,r-expm) ("r-fnn" ,r-fnn) ("r-forcats" ,r-forcats) ("r-future" ,r-future) ("r-future-apply" ,r-future-apply) ("r-gg-gap" ,r-gg-gap) ("r-ggalluvial" ,r-ggalluvial) ("r-ggplot2" ,r-ggplot2) ("r-ggrepel" ,r-ggrepel) ("r-igraph" ,r-igraph) ("r-irlba" ,r-irlba) ("r-magrittr" ,r-magrittr) ("r-matrix" ,r-matrix) ("r-nmf" ,r-nmf) ("r-patchwork" ,r-patchwork) ("r-pbapply" ,r-pbapply) ("r-rcolorbrewer" ,r-rcolorbrewer) ("r-rcpp" ,r-rcpp) ("r-rcppeigen" ,r-rcppeigen) ("r-reshape2" ,r-reshape2) ("r-reticulate" ,r-reticulate) ("r-rspectra" ,r-rspectra) ("r-rtsne" ,r-rtsne) ("r-scales" ,r-scales) ("r-shape" ,r-shape) ("r-sna" ,r-sna) ("r-stringr" ,r-stringr) ("r-svglite" ,r-svglite))) (native-inputs `(("r-knitr" ,r-knitr))) (home-page "https://github.com/sqjin/CellChat") (synopsis "Analysis of cell-cell communication from single-cell transcriptomics data") (description "This package infers, visualizes and analyzes the cell-cell communication networks from scRNA-seq data.") (license license:gpl3)))) (define-public sambamba (package (name "sambamba") (version "0.8.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/biod/sambamba") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "07dznzl6m8k7sw84jxw2kx6i3ymrapbmcmyh0fxz8wrybhw8fmwc")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there is no test target #:parallel-build? #f ; not supported #:phases (modify-phases %standard-phases (delete 'configure) (add-after 'unpack 'fix-ldc-version (lambda _ (substitute* "Makefile" ;; We use ldc2 instead of ldmd2 to compile sambamba. (("\\$\\(shell which ldmd2\\)") (which "ldc2"))) #t)) (add-after 'unpack 'unbundle-prerequisites (lambda _ (substitute* "Makefile" (("= lz4/lib/liblz4.a") "= -L-llz4") (("ldc_version_info lz4-static") "ldc_version_info")) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p bin) (copy-file (string-append "bin/sambamba-" ,version) (string-append bin "/sambamba")) #t)))))) (native-inputs `(("python" ,python))) (inputs `(("ldc" ,ldc) ("lz4" ,lz4) ("zlib" ,zlib))) (home-page "https://github.com/biod/sambamba") (synopsis "Tools for working with SAM/BAM data") (description "Sambamba is a high performance modern robust and fast tool (and library), written in the D programming language, for working with SAM and BAM files. Current parallelised functionality is an important subset of samtools functionality, including view, index, sort, markdup, and depth.") (license license:gpl2+))) (define-public ritornello (package (name "ritornello") (version "2.0.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/KlugerLab/Ritornello") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1xahvq215qld7x1w8vpa5zbrsj6p9crb9shqa2x89sb0aaxa02jk")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are no tests #:phases (modify-phases %standard-phases (add-after 'unpack 'patch-samtools-references (lambda* (#:key inputs #:allow-other-keys) (substitute* '("src/SamStream.h" "src/FLD.cpp") (("") "")) #t)) (delete 'configure) (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin/"))) (mkdir-p bin) (install-file "bin/Ritornello" bin) #t)))))) (inputs `(("samtools" ,samtools-0.1) ("fftw" ,fftw) ("boost" ,boost) ("zlib" ,zlib))) (home-page "https://github.com/KlugerLab/Ritornello") (synopsis "Control-free peak caller for ChIP-seq data") (description "Ritornello is a ChIP-seq peak calling algorithm based on signal processing that can accurately call binding events without the need to do a pair total DNA input or IgG control sample. It has been tested for use with narrow binding events such as transcription factor ChIP-seq.") (license license:gpl3+))) (define-public trim-galore (package (name "trim-galore") (version "0.6.6") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/FelixKrueger/TrimGalore") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "0yrwg6325j4sb9vnplvl3jplzab0qdhp92wl480qjinpfq88j4rs")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no tests #:phases (modify-phases %standard-phases (replace 'configure (lambda _ ;; Trim Galore tries to figure out what version of Python ;; cutadapt is using by looking at the shebang. Of course that ;; doesn't work, because cutadapt is wrapped in a shell script. (substitute* "trim_galore" (("my \\$python_return.*") "my $python_return = \"Python 3.999\";\n")) #t)) (delete 'build) (add-after 'unpack 'hardcode-tool-references (lambda* (#:key inputs #:allow-other-keys) (substitute* "trim_galore" (("\\$path_to_cutadapt = 'cutadapt'") (string-append "$path_to_cutadapt = '" (assoc-ref inputs "cutadapt") "/bin/cutadapt'")) (("\\$compression_path = \"gzip\"") (string-append "$compression_path = \"" (assoc-ref inputs "gzip") "/bin/gzip\"")) (("\"gunzip") (string-append "\"" (assoc-ref inputs "gzip") "/bin/gunzip")) (("\"pigz") (string-append "\"" (assoc-ref inputs "pigz") "/bin/pigz"))) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p bin) (install-file "trim_galore" bin) #t)))))) (inputs `(("gzip" ,gzip) ("perl" ,perl) ("pigz" ,pigz) ("cutadapt" ,cutadapt))) (native-inputs `(("unzip" ,unzip))) (home-page "https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/") (synopsis "Wrapper around Cutadapt and FastQC") (description "Trim Galore! is a wrapper script to automate quality and adapter trimming as well as quality control, with some added functionality to remove biased methylation positions for RRBS sequence files.") (license license:gpl3+))) (define-public gess (package (name "gess") (version "1.0") (source (origin (method url-fetch) (uri (string-append "http://compbio.uthscsa.edu/" "GESS_Web/files/" "gess-" version ".src.tar.gz")) (sha256 (base32 "0hyk403kxscclzfs24pvdgiv0wm03kjcziqdrp5w46cb049gz0d7")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no tests #:phases (modify-phases %standard-phases (delete 'configure) (delete 'build) (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((python (assoc-ref inputs "python")) (out (assoc-ref outputs "out")) (bin (string-append out "/bin/")) (target (string-append out "/lib/python" ,(version-major+minor (package-version python)) "/site-packages/gess/"))) (mkdir-p target) (copy-recursively "." target) ;; Make GESS.py executable (chmod (string-append target "GESS.py") #o555) ;; Add Python shebang to the top and make Matplotlib ;; usable. (substitute* (string-append target "GESS.py") (("\"\"\"Description:" line) (string-append "#!" (which "python") " import matplotlib matplotlib.use('Agg') " line))) ;; Make sure GESS has all modules in its path (wrap-script (string-append target "GESS.py") `("PYTHONPATH" ":" = (,target ,(getenv "PYTHONPATH")))) (mkdir-p bin) (symlink (string-append target "GESS.py") (string-append bin "GESS.py")) #t)))))) (inputs `(("python" ,python-2) ("python2-pysam" ,python2-pysam) ("python2-scipy" ,python2-scipy) ("python2-numpy" ,python2-numpy) ("python2-networkx" ,python2-networkx) ("python2-biopython" ,python2-biopython) ("guile" ,guile-3.0))) ; for the script wrapper (home-page "https://compbio.uthscsa.edu/GESS_Web/") (synopsis "Detect exon-skipping events from raw RNA-seq data") (description "GESS is an implementation of a novel computational method to detect de novo exon-skipping events directly from raw RNA-seq data without the prior knowledge of gene annotation information. GESS stands for the graph-based exon-skipping scanner detection scheme.") (license license:bsd-3))) (define-public phylip (package (name "phylip") (version "3.696") (source (origin (method url-fetch) (uri (string-append "http://evolution.gs.washington.edu/phylip/" "download/phylip-" version ".tar.gz")) (sha256 (base32 "01jar1rayhr2gba2pgbw49m56rc5z4p5wn3ds0m188hrlln4a2nd")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no check target #:make-flags (list "-f" "Makefile.unx" "install") #:parallel-build? #f ; not supported #:phases (modify-phases %standard-phases (add-after 'unpack 'enter-dir (lambda _ (chdir "src") #t)) (delete 'configure) (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let ((target (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p target) (for-each (lambda (file) (install-file file target)) (find-files "../exe" ".*"))) #t))))) (home-page "http://evolution.genetics.washington.edu/phylip/") (synopsis "Tools for inferring phylogenies") (description "PHYLIP (the PHYLogeny Inference Package) is a package of programs for inferring phylogenies (evolutionary trees).") (license license:bsd-2))) (define-public imp (package (name "imp") (version "2.13.0") (source (origin (method url-fetch) (uri (string-append "https://integrativemodeling.org/" version "/download/imp-" version ".tar.gz")) (sha256 (base32 "1z1vcpwbylixk0zywngg5iw0jv083jj1bqphi817jpg3fb9fx2jj")))) (build-system cmake-build-system) (arguments `( ;; CMake 3.17 or newer is required for the CMAKE_TEST_ARGUMENTS used ;; below to have an effect. #:cmake ,cmake #:configure-flags (let ((disabled-tests '("expensive" ;exclude expensive tests "IMP.modeller" ;fail to import its own modules "IMP.parallel-test_sge.py" ;fail in build container ;; The following test fails non-reproducibly on ;; an inexact numbers assertion. "IMP.em-medium_test_local_fitting.py"))) (list (string-append "-DCMAKE_CTEST_ARGUMENTS=" (string-join (list "-L" "-tests?-" ;select only tests "-E" (format #f "'(~a)'" (string-join disabled-tests "|"))) ";")))))) (native-inputs `(("python" ,python-wrapper) ("swig" ,swig))) (inputs `(("boost" ,boost) ("cgal" ,cgal) ("gsl" ,gsl) ("hdf5" ,hdf5) ("fftw" ,fftw) ("eigen" ,eigen) ;; Enabling MPI causes the build to use all the available memory and ;; fail (tested on a machine with 32 GiB of RAM). ;;("mpi" ,openmpi) ("opencv" ,opencv))) (propagated-inputs `(("python-numpy" ,python-numpy) ("python-scipy" ,python-scipy) ("python-pandas" ,python-pandas) ("python-scikit-learn" ,python-scikit-learn) ("python-networkx" ,python-networkx))) (home-page "https://integrativemodeling.org") (synopsis "Integrative modeling platform") (description "IMP's broad goal is to contribute to a comprehensive structural characterization of biomolecules ranging in size and complexity from small peptides to large macromolecular assemblies, by integrating data from diverse biochemical and biophysical experiments. IMP provides a C++ and Python toolbox for solving complex modeling problems, and a number of applications for tackling some common problems in a user-friendly way.") ;; IMP is largely available under the GNU Lesser GPL; see the file ;; COPYING.LGPL for the full text of this license. Some IMP modules are ;; available under the GNU GPL (see the file COPYING.GPL). (license (list license:lgpl2.1+ license:gpl3+)))) (define-public tadbit (package (name "tadbit") (version "1.0.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/3DGenomes/TADbit") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0hqrlymh2a2bimcfdvlssy1x5h1lp3h1c5a7jj11hmcqczzqn3ni")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'fix-problems-with-setup.py (lambda* (#:key outputs #:allow-other-keys) ;; Don't attempt to install the bash completions to ;; the home directory. (rename-file "extras/.bash_completion" "extras/tadbit") (substitute* "setup.py" (("\\(path.expanduser\\('~'\\)") (string-append "(\"" (assoc-ref outputs "out") "/etc/bash_completion.d\"")) (("extras/\\.bash_completion") "extras/tadbit")) #t)) (replace 'check (lambda* (#:key inputs outputs #:allow-other-keys) (add-installed-pythonpath inputs outputs) (invoke "python3" "test/test_all.py") #t))))) (native-inputs `(("glib" ,glib "bin") ;for gtester ("pkg-config" ,pkg-config))) (inputs ;; TODO: add Chimera for visualization `(("imp" ,imp) ("mcl" ,mcl) ("python-future" ,python-future) ("python-h5py" ,python-h5py) ("python-scipy" ,python-scipy) ("python-numpy" ,python-numpy) ("python-matplotlib" ,python-matplotlib) ("python-pysam" ,python-pysam))) (home-page "https://3dgenomes.github.io/TADbit/") (synopsis "Analyze, model, and explore 3C-based data") (description "TADbit is a complete Python library to deal with all steps to analyze, model, and explore 3C-based data. With TADbit the user can map FASTQ files to obtain raw interaction binned matrices (Hi-C like matrices), normalize and correct interaction matrices, identify and compare the so-called @dfn{Topologically Associating Domains} (TADs), build 3D models from the interaction matrices, and finally, extract structural properties from the models. TADbit is complemented by TADkit for visualizing 3D models.") (license license:gpl3+))) (define-public kentutils (package (name "kentutils") ;; 302.1.0 is out, but the only difference is the inclusion of ;; pre-built binaries. (version "302.0.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ENCODE-DCC/kentUtils") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0n1wbyjpzii2b9qhyp9r1q76j623cggpg3y8fmw78ld3z4y7ivha")) (modules '((guix build utils) (srfi srfi-26) (ice-9 ftw))) (snippet '(begin ;; Only the contents of the specified directories are free ;; for all uses, so we remove the rest. "hg/autoSql" and ;; "hg/autoXml" are nominally free, but they depend on a ;; library that is built from the sources in "hg/lib", ;; which is nonfree. (let ((free (list "." ".." "utils" "lib" "inc" "tagStorm" "parasol" "htslib")) (directory? (lambda (file) (eq? 'directory (stat:type (stat file)))))) (for-each (lambda (file) (and (directory? file) (delete-file-recursively file))) (map (cut string-append "src/" <>) (scandir "src" (lambda (file) (not (member file free))))))) ;; Only make the utils target, not the userApps target, ;; because that requires libraries we won't build. (substitute* "Makefile" ((" userApps") " utils")) ;; Only build libraries that are free. (substitute* "src/makefile" (("DIRS =.*") "DIRS =\n") (("cd jkOwnLib.*") "") ((" hgLib") "") (("cd hg.*") "")) (substitute* "src/utils/makefile" ;; These tools depend on "jkhgap.a", which is part of the ;; nonfree "src/hg/lib" directory. (("raSqlQuery") "") (("pslLiftSubrangeBlat") "") ;; Do not build UCSC tools, which may require nonfree ;; components. (("ALL_APPS =.*") "ALL_APPS = $(UTILS_APPLIST)\n")) #t)))) (build-system gnu-build-system) (arguments `( ;; There is no global test target and the test target for ;; individual tools depends on input files that are not ;; included. #:tests? #f #:phases (modify-phases %standard-phases (add-after 'unpack 'fix-permissions (lambda _ (make-file-writable "src/inc/localEnvironment.mk") #t)) (add-after 'unpack 'fix-paths (lambda _ (substitute* "Makefile" (("/bin/echo") (which "echo"))) #t)) (add-after 'unpack 'prepare-samtabix (lambda* (#:key inputs #:allow-other-keys) (copy-recursively (assoc-ref inputs "samtabix") "samtabix") #t)) (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (copy-recursively "bin" bin)) #t))))) (native-inputs `(("samtabix" ,(let ((commit "10fd107909c1ac4d679299908be4262a012965ba")) (origin (method git-fetch) (uri (git-reference (url "http://genome-source.cse.ucsc.edu/samtabix.git") (commit commit))) (file-name (git-file-name "samtabix" (string-take commit 7))) (sha256 (base32 "0c1nj64l42v395sa84n7az43xiap4i6f9n9dfz4058aqiwkhkmma"))))))) (inputs `(("zlib" ,zlib) ("tcsh" ,tcsh) ("perl" ,perl) ("libpng" ,libpng) ("mariadb-dev" ,mariadb "dev") ("openssl" ,openssl))) (home-page "https://genome.cse.ucsc.edu/index.html") (synopsis "Assorted bioinformatics utilities") (description "This package provides the kentUtils, a selection of bioinformatics utilities used in combination with the UCSC genome browser.") ;; Only a subset of the sources are released under a non-copyleft ;; free software license. All other sources are removed in a ;; snippet. See this bug report for an explanation of how the ;; license statements apply: ;; https://github.com/ENCODE-DCC/kentUtils/issues/12 (license (license:non-copyleft "http://genome.ucsc.edu/license/" "The contents of this package are free for all uses.")))) (define-public f-seq (let ((commit "6ccded34cff38cf432deed8503648b4a66953f9b") (revision "1")) (package (name "f-seq") (version (git-version "1.1" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/aboyle/F-seq") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "1nk33k0yajg2id4g59bc4szr58r2q6pdq42vgcw054m8ip9wv26h")) (modules '((guix build utils))) ;; Remove bundled Java library archives. (snippet '(begin (for-each delete-file (find-files "lib" ".*")) #t)))) (build-system ant-build-system) (arguments `(#:tests? #f ; no tests included #:phases (modify-phases %standard-phases (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((target (assoc-ref outputs "out")) (bin (string-append target "/bin")) (doc (string-append target "/share/doc/f-seq")) (lib (string-append target "/lib"))) (mkdir-p target) (mkdir-p doc) (substitute* "bin/linux/fseq" (("java") (which "java")) (("\\$REALDIR/../lib/commons-cli-1.1.jar") (string-append (assoc-ref inputs "java-commons-cli") "/share/java/commons-cli.jar")) (("REALDIR=.*") (string-append "REALDIR=" bin "\n"))) (install-file "README.txt" doc) (install-file "bin/linux/fseq" bin) (install-file "build~/fseq.jar" lib) (copy-recursively "lib" lib) #t)))))) (inputs `(("perl" ,perl) ("java-commons-cli" ,java-commons-cli))) (home-page "https://fureylab.web.unc.edu/software/fseq/") (synopsis "Feature density estimator for high-throughput sequence tags") (description "F-Seq is a software package that generates a continuous tag sequence density estimation allowing identification of biologically meaningful sites such as transcription factor binding sites (ChIP-seq) or regions of open chromatin (DNase-seq). Output can be displayed directly in the UCSC Genome Browser.") (license license:gpl3+)))) (define-public bismark (package (name "bismark") (version "0.20.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/FelixKrueger/Bismark") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "0xchm3rgilj6vfjnyzfzzymfd7djr64sbrmrvs3njbwi66jqbzw9")))) (build-system perl-build-system) (arguments `(#:tests? #f ; there are no tests #:modules ((guix build utils) (ice-9 popen) (srfi srfi-26) (guix build perl-build-system)) #:phases (modify-phases %standard-phases ;; The bundled plotly.js is minified. (add-after 'unpack 'replace-plotly.js (lambda* (#:key inputs #:allow-other-keys) (let* ((file (assoc-ref inputs "plotly.js")) (installed "plotly/plotly.js")) (let ((minified (open-pipe* OPEN_READ "uglify-js" file))) (call-with-output-file installed (cut dump-port minified <>)))) #t)) (delete 'configure) (delete 'build) (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (share (string-append out "/share/bismark")) (docdir (string-append out "/share/doc/bismark")) (docs '("Docs/Bismark_User_Guide.html")) (scripts '("bismark" "bismark_genome_preparation" "bismark_methylation_extractor" "bismark2bedGraph" "bismark2report" "coverage2cytosine" "deduplicate_bismark" "filter_non_conversion" "bam2nuc" "bismark2summary" "NOMe_filtering"))) (substitute* "bismark2report" (("\\$RealBin/plotly") (string-append share "/plotly"))) (mkdir-p share) (mkdir-p docdir) (mkdir-p bin) (for-each (lambda (file) (install-file file bin)) scripts) (for-each (lambda (file) (install-file file docdir)) docs) (copy-recursively "Docs/Images" (string-append docdir "/Images")) (copy-recursively "plotly" (string-append share "/plotly")) ;; Fix references to gunzip (substitute* (map (lambda (file) (string-append bin "/" file)) scripts) (("\"gunzip -c") (string-append "\"" (assoc-ref inputs "gzip") "/bin/gunzip -c"))) #t)))))) (inputs `(("gzip" ,gzip) ("perl-carp" ,perl-carp) ("perl-getopt-long" ,perl-getopt-long))) (native-inputs `(("plotly.js" ,(origin (method url-fetch) (uri (string-append "https://raw.githubusercontent.com/plotly/plotly.js/" "v1.39.4/dist/plotly.js")) (sha256 (base32 "138mwsr4nf5qif4mrxx286mpnagxd1xwl6k8aidrjgknaqg88zyr")))) ("uglify-js" ,uglify-js))) (home-page "https://www.bioinformatics.babraham.ac.uk/projects/bismark/") (synopsis "Map bisulfite treated sequence reads and analyze methylation") (description "Bismark is a program to map bisulfite treated sequencing reads to a genome of interest and perform methylation calls in a single step. The output can be easily imported into a genome viewer, such as SeqMonk, and enables a researcher to analyse the methylation levels of their samples straight away. Its main features are: @itemize @item Bisulfite mapping and methylation calling in one single step @item Supports single-end and paired-end read alignments @item Supports ungapped and gapped alignments @item Alignment seed length, number of mismatches etc are adjustable @item Output discriminates between cytosine methylation in CpG, CHG and CHH context @end itemize\n") (license license:gpl3+))) (define-public paml (package (name "paml") (version "4.9e") (source (origin (method url-fetch) (uri (string-append "http://abacus.gene.ucl.ac.uk/software/" "paml" version ".tgz")) (sha256 (base32 "13zf6h9fiqghwhch2h06x1zdr6s42plsnqahflp5g7myr3han3s6")) (modules '((guix build utils))) ;; Remove Windows binaries (snippet '(begin (for-each delete-file (find-files "." "\\.exe$")) ;; Some files in the original tarball have restrictive ;; permissions, which makes repackaging fail (for-each (lambda (file) (chmod file #o644)) (find-files ".")) #t)))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are no tests #:make-flags '("CC=gcc") #:phases (modify-phases %standard-phases (replace 'configure (lambda _ (substitute* "src/BFdriver.c" (("/bin/bash") (which "bash"))) (chdir "src") #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((tools '("baseml" "basemlg" "codeml" "pamp" "evolver" "yn00" "chi2")) (bin (string-append (assoc-ref outputs "out") "/bin")) (docdir (string-append (assoc-ref outputs "out") "/share/doc/paml"))) (mkdir-p bin) (for-each (lambda (file) (install-file file bin)) tools) (copy-recursively "../doc" docdir) #t)))))) (home-page "http://abacus.gene.ucl.ac.uk/software/paml.html") (synopsis "Phylogentic analysis by maximum likelihood") (description "PAML (for Phylogentic Analysis by Maximum Likelihood) contains a few programs for model fitting and phylogenetic tree reconstruction using nucleotide or amino-acid sequence data.") ;; GPLv3 only (license license:gpl3))) (define-public kallisto (package (name "kallisto") (version "0.46.2") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/pachterlab/kallisto") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0ij5n7v3m90jdfi7sn8nvglfyf58abp1f5xq42r4k73l0lfds6xi")) (modules '((guix build utils))) (snippet '(delete-file-recursively "ext/htslib/")))) (build-system cmake-build-system) (arguments `(#:tests? #f ; no "check" target #:phases (modify-phases %standard-phases (add-after 'unpack 'do-not-use-bundled-htslib (lambda _ (substitute* "CMakeLists.txt" (("^ExternalProject_Add" m) (string-append "if (NEVER)\n" m)) (("^\\)") (string-append ")\nendif(NEVER)")) (("include_directories\\(\\$\\{htslib_PREFIX.*" m) (string-append "# " m))) (substitute* "src/CMakeLists.txt" (("target_link_libraries\\(kallisto kallisto_core pthread \ \\$\\{CMAKE_CURRENT_SOURCE_DIR\\}/../ext/htslib/libhts.a\\)") "target_link_libraries(kallisto kallisto_core pthread hts)") (("include_directories\\(\\.\\./ext/htslib\\)") "")) #t))))) (inputs `(("hdf5" ,hdf5) ("htslib" ,htslib-1.9) ("zlib" ,zlib))) (home-page "https://pachterlab.github.io/kallisto/") (synopsis "Near-optimal RNA-Seq quantification") (description "Kallisto is a program for quantifying abundances of transcripts from RNA-Seq data, or more generally of target sequences using high-throughput sequencing reads. It is based on the novel idea of pseudoalignment for rapidly determining the compatibility of reads with targets, without the need for alignment. Pseudoalignment of reads preserves the key information needed for quantification, and kallisto is therefore not only fast, but also as accurate as existing quantification tools.") (license license:bsd-2))) (define-public libgff (package (name "libgff") (version "2.0.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/COMBINE-lab/libgff") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0ds9r22y8bl1rj7bhl0003kgmm6aam7g8l41mnjfrzw15d9zf9k4")))) (build-system cmake-build-system) (arguments `(#:tests? #f)) ; no tests included (home-page "https://github.com/COMBINE-lab/libgff") (synopsis "Parser library for reading/writing GFF files") (description "This is a simple \"libraryfication\" of the GFF/GTF parsing code that is used in the Cufflinks codebase. The goal of this library is to provide this functionality without the necessity of drawing in a heavy-weight dependency like SeqAn.") (license (license:x11-style "https://www.boost.org/LICENSE_1_0.txt")))) (define-public sailfish (package (name "sailfish") (version "0.10.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/kingsfordgroup/sailfish") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1amcc5hqvsl42hg4x19bi9vy47cl874s0lw1fmi0hwsdk9i8c03v")) (modules '((guix build utils))) (snippet '(begin ;; Delete bundled headers for eigen3. (delete-file-recursively "include/eigen3/") #t)))) (build-system cmake-build-system) (arguments `(#:configure-flags (list (string-append "-DBOOST_INCLUDEDIR=" (assoc-ref %build-inputs "boost") "/include/") (string-append "-DBOOST_LIBRARYDIR=" (assoc-ref %build-inputs "boost") "/lib/") (string-append "-DBoost_LIBRARIES=" "-lboost_iostreams " "-lboost_filesystem " "-lboost_system " "-lboost_thread " "-lboost_timer " "-lboost_chrono " "-lboost_program_options") "-DBoost_FOUND=TRUE" ;; Don't download RapMap---we already have it! "-DFETCHED_RAPMAP=1") ;; Tests must be run after installation and the location of the test ;; data file must be overridden. But the tests fail. It looks like ;; they are not really meant to be run. #:tests? #f #:phases (modify-phases %standard-phases ;; Boost cannot be found, even though it's right there. (add-after 'unpack 'do-not-look-for-boost (lambda* (#:key inputs #:allow-other-keys) (substitute* "CMakeLists.txt" (("find_package\\(Boost 1\\.53\\.0") "#")) #t)) (add-after 'unpack 'do-not-assign-to-macro (lambda _ (substitute* "include/spdlog/details/format.cc" (("const unsigned CHAR_WIDTH = 1;") "")) #t)) (add-after 'unpack 'prepare-rapmap (lambda* (#:key inputs #:allow-other-keys) (let ((src "external/install/src/rapmap/") (include "external/install/include/rapmap/") (rapmap (assoc-ref inputs "rapmap"))) (mkdir-p "/tmp/rapmap") (invoke "tar" "xf" (assoc-ref inputs "rapmap") "-C" "/tmp/rapmap" "--strip-components=1") (mkdir-p src) (mkdir-p include) (for-each (lambda (file) (install-file file src)) (find-files "/tmp/rapmap/src" "\\.(c|cpp)")) (copy-recursively "/tmp/rapmap/include" include)) #t)) (add-after 'unpack 'use-system-libraries (lambda* (#:key inputs #:allow-other-keys) (substitute* '("src/SailfishIndexer.cpp" "src/SailfishUtils.cpp" "src/SailfishQuantify.cpp" "src/FASTAParser.cpp" "include/PCA.hpp" "include/SailfishUtils.hpp" "include/SailfishIndex.hpp" "include/CollapsedEMOptimizer.hpp" "src/CollapsedEMOptimizer.cpp") (("#include \"jellyfish/config.h\"") "")) (substitute* "src/CMakeLists.txt" (("\\$\\{GAT_SOURCE_DIR\\}/external/install/include/jellyfish-2.2..") (string-append (assoc-ref inputs "jellyfish") "/include/jellyfish-" ,(package-version jellyfish))) (("\\$\\{GAT_SOURCE_DIR\\}/external/install/lib/libjellyfish-2.0.a") (string-append (assoc-ref inputs "jellyfish") "/lib/libjellyfish-2.0.a")) (("\\$\\{GAT_SOURCE_DIR\\}/external/install/lib/libdivsufsort.a") (string-append (assoc-ref inputs "libdivsufsort") "/lib/libdivsufsort.so")) (("\\$\\{GAT_SOURCE_DIR\\}/external/install/lib/libdivsufsort64.a") (string-append (assoc-ref inputs "libdivsufsort") "/lib/libdivsufsort64.so"))) (substitute* "CMakeLists.txt" ;; Don't prefer static libs (("SET\\(CMAKE_FIND_LIBRARY_SUFFIXES.*") "") (("find_package\\(Jellyfish.*") "") (("ExternalProject_Add\\(libjellyfish") "message(") (("ExternalProject_Add\\(libgff") "message(") (("ExternalProject_Add\\(libsparsehash") "message(") (("ExternalProject_Add\\(libdivsufsort") "message(")) ;; Ensure that Eigen headers can be found (setenv "CPLUS_INCLUDE_PATH" (string-append (assoc-ref inputs "eigen") "/include/eigen3:" (or (getenv "CPLUS_INCLUDE_PATH") ""))) #t))))) (inputs `(("boost" ,boost) ("eigen" ,eigen) ("jemalloc" ,jemalloc) ("jellyfish" ,jellyfish) ("sparsehash" ,sparsehash) ("rapmap" ,(origin (method git-fetch) (uri (git-reference (url "https://github.com/COMBINE-lab/RapMap") (commit (string-append "sf-v" version)))) (file-name (string-append "rapmap-sf-v" version "-checkout")) (sha256 (base32 "1hv79l5i576ykv5a1srj2p0q36yvyl5966m0fcy2lbi169ipjakf")) (modules '((guix build utils))) ;; These files are expected to be excluded. (snippet '(begin (delete-file-recursively "include/spdlog") (for-each delete-file '("include/xxhash.h" "src/xxhash.c")) #t)))) ("libdivsufsort" ,libdivsufsort) ("libgff" ,libgff) ("tbb" ,tbb) ("zlib" ,zlib))) (native-inputs `(("pkg-config" ,pkg-config))) (home-page "https://www.cs.cmu.edu/~ckingsf/software/sailfish/") (synopsis "Mapping-based isoform quantification from RNA-Seq reads") (description "Sailfish is a tool for genomic transcript quantification from RNA-seq data. It requires a set of target transcripts (either from a reference or de-novo assembly) to quantify. All you need to run sailfish is a fasta file containing your reference transcripts and a (set of) fasta/fastq file(s) containing your reads.") (license license:gpl3+))) (define libstadenio-for-salmon (package (name "libstadenio") (version "1.14.8") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/COMBINE-lab/staden-io_lib") (commit (string-append "v" version)))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "1x8kxxqxl892vwfbprlbyfwkkv7c34ggkc94892x9x0g37x5nbwx")))) (build-system gnu-build-system) (arguments '(#:parallel-tests? #f)) ; not supported (inputs `(("zlib" ,zlib))) (native-inputs `(("perl" ,perl))) ; for tests (home-page "https://github.com/COMBINE-lab/staden-io_lib") (synopsis "General purpose trace and experiment file library") (description "This package provides a library of file reading and writing code to provide a general purpose Trace file (and Experiment File) reading interface. The following file formats are supported: @enumerate @item SCF trace files @item ABI trace files @item ALF trace files @item ZTR trace files @item SFF trace archives @item SRF trace archives @item Experiment files @item Plain text files @item SAM/BAM sequence files @item CRAM sequence files @end enumerate\n") (license license:bsd-3))) (define-public salmon (package (name "salmon") (version "1.4.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/COMBINE-lab/salmon") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1di7y2s8cjr9480lngcmaz3wcabc1lpkyanzbhir1nkhcjmj70h4")) (modules '((guix build utils))) (snippet ;; Delete bundled headers for eigen3. '(delete-file-recursively "include/eigen3/")))) (build-system cmake-build-system) (arguments `(#:configure-flags (list (string-append "-Dlibgff_DIR=" (assoc-ref %build-inputs "libgff") "/lib") "-Dlibgff_FOUND=TRUE" "-DTBB_FOUND=TRUE" "-DTBB_VERSION=2020.3" "-DTBB_LIBRARIES=tbb -ltbbmalloc" "-DFETCHED_PUFFERFISH=TRUE" "-DUSE_SHARED_LIBS=TRUE") #:phases (modify-phases %standard-phases (add-after 'unpack 'prepare-pufferfish (lambda* (#:key inputs #:allow-other-keys) (copy-recursively (assoc-ref inputs "pufferfish") "external/pufferfish") ;; This test isn't working correctly, so compilation aborts. (substitute* "external/pufferfish/include/string_view.hpp" (("#if __has_include\\(\\)") "#if 0")) (let ((headers "external/install/pufferfish/include/pufferfish") (source "external/install/src/pufferfish")) (mkdir-p headers) (mkdir-p source) (for-each (lambda (file) (install-file (string-append "external/pufferfish/include/" file) headers)) (list "ProgOpts.hpp" "BooPHF.hpp" "SpinLock.hpp" "Kmer.hpp" "CanonicalKmer.hpp" "string_view.hpp" "CanonicalKmerIterator.hpp" "PufferfishBaseIndex.hpp" "PufferfishIndex.hpp" "PufferfishSparseIndex.hpp" "PufferfishLossyIndex.hpp" "PufferfishTypes.hpp" "rank9b.hpp" "rank9sel.hpp" "macros.hpp" "select.hpp" "Util.hpp" "PairedAlignmentFormatter.hpp" "SelectiveAlignmentUtils.hpp" "PuffAligner.hpp" "MemCollector.hpp" "MemChainer.hpp" "CommonTypes.hpp" "SAMWriter.hpp" "PufferfishConfig.hpp" "BulkChunk.hpp" "BinWriter.hpp")) (for-each (lambda (dir) (copy-recursively (string-append "external/pufferfish/include/" dir) (string-append headers "/" dir))) (list "libdivide" "ksw2pp" "compact_vector" "metro" "chobo" "sparsepp" "simde" "tsl")) (copy-recursively (string-append "external/pufferfish/src/metro/") (string-append source "/metro")) (install-file (string-append "external/pufferfish/src/rank9b.cpp") source) ;; Do not complain about not having built libtbb (substitute* "external/pufferfish/external/twopaco/CMakeLists.txt" (("add_dependencies.*") ""))))) (add-after 'unpack 'do-not-phone-home (lambda _ (substitute* "src/Salmon.cpp" (("getVersionMessage\\(\\)") "\"\"")))) (add-after 'unpack 'use-system-libraries (lambda* (#:key inputs #:allow-other-keys) ;; Ensure that all headers can be found (setenv "CPLUS_INCLUDE_PATH" (string-append (or (getenv "CPLUS_INCLUDE_PATH") "") ":" (getcwd) "/external/install/pufferfish/include:" (assoc-ref inputs "eigen") "/include/eigen3")))) (add-after 'unpack 'fix-error-message-in-tests (lambda _ (substitute* "cmake/TestSalmonQuasi.cmake" (("SALMON_QUASI_INDEX_COMMAND") "SALMON_QUASI_INDEX_CMD"))))))) (inputs `(("boost" ,boost) ("bzip2" ,bzip2) ("cereal" ,cereal) ("curl" ,curl) ("eigen" ,eigen) ("jemalloc" ,jemalloc) ("libgff" ,libgff) ("pufferfish" ,(origin (method git-fetch) (uri (git-reference (url "https://github.com/COMBINE-lab/pufferfish") (commit (string-append "salmon-v" version)))) (file-name (git-file-name "pufferfish" version)) (sha256 (base32 "0qb4a2nl1d59qasr17sslgxnkjd5kbk5mns4cjshrmsvkrqp995n")))) ("tbb" ,tbb) ("libstadenio-for-salmon" ,libstadenio-for-salmon) ("xz" ,xz) ("zlib" ,zlib))) (native-inputs `(("pkg-config" ,pkg-config))) (home-page "https://github.com/COMBINE-lab/salmon") (synopsis "Quantification from RNA-seq reads using lightweight alignments") (description "Salmon is a program to produce highly-accurate, transcript-level quantification estimates from RNA-seq data. Salmon achieves its accuracy and speed via a number of different innovations, including the use of lightweight alignments (accurate but fast-to-compute proxies for traditional read alignments) and massively-parallel stochastic collapsed variational inference.") (license license:gpl3+))) (define-public python-loompy (package (name "python-loompy") (version "2.0.17") ;; The tarball on Pypi does not include the tests. (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/linnarsson-lab/loompy") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "12a5kjgiikapv93wahfw0frszx1lblnppyz3vs5gy8fgmgngra07")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (replace 'check (lambda _ (setenv "PYTHONPATH" (string-append (getcwd) ":" (getenv "PYTHONPATH"))) (invoke "pytest" "tests") #t))))) (propagated-inputs `(("python-h5py" ,python-h5py) ("python-numpy" ,python-numpy) ("python-pandas" ,python-pandas) ("python-scipy" ,python-scipy))) (native-inputs `(("python-pytest" ,python-pytest))) (home-page "https://github.com/linnarsson-lab/loompy") (synopsis "Work with .loom files for single-cell RNA-seq data") (description "The loom file format is an efficient format for very large omics datasets, consisting of a main matrix, optional additional layers, a variable number of row and column annotations. Loom also supports sparse graphs. This library makes it easy to work with @file{.loom} files for single-cell RNA-seq data.") (license license:bsd-3))) ;; We cannot use the latest commit because it requires Java 9. (define-public java-forester (let ((commit "86b07efe302d5094b42deed9260f719a4c4ac2e6") (revision "1")) (package (name "java-forester") (version (string-append "0-" revision "." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/cmzmasek/forester") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "0vxavc1yrf84yrnf20dq26hi0lglidk8d382xrxsy4qmlbjd276z")) (modules '((guix build utils))) (snippet '(begin ;; Delete bundled jars and pre-built classes (delete-file-recursively "forester/java/resources") (delete-file-recursively "forester/java/classes") (for-each delete-file (find-files "forester/java/" "\\.jar$")) ;; Delete bundled applications (delete-file-recursively "forester_applications") #t)))) (build-system ant-build-system) (arguments `(#:tests? #f ; there are none #:jdk ,icedtea-8 #:modules ((guix build ant-build-system) (guix build utils) (guix build java-utils) (sxml simple) (sxml transform)) #:phases (modify-phases %standard-phases (add-after 'unpack 'chdir (lambda _ (chdir "forester/java") #t)) (add-after 'chdir 'fix-dependencies (lambda _ (chmod "build.xml" #o664) (call-with-output-file "build.xml.new" (lambda (port) (sxml->xml (pre-post-order (with-input-from-file "build.xml" (lambda _ (xml->sxml #:trim-whitespace? #t))) `(;; Remove all unjar tags to avoid repacking classes. (unjar . ,(lambda _ '())) (*default* . ,(lambda (tag . kids) `(,tag ,@kids))) (*text* . ,(lambda (_ txt) txt)))) port))) (rename-file "build.xml.new" "build.xml") #t)) ;; FIXME: itext is difficult to package as it depends on a few ;; unpackaged libraries. (add-after 'chdir 'remove-dependency-on-unpackaged-itext (lambda _ (delete-file "src/org/forester/archaeopteryx/PdfExporter.java") (substitute* "src/org/forester/archaeopteryx/MainFrame.java" (("pdf_written_to = PdfExporter.*") "throw new IOException(\"PDF export is not available.\");")) #t)) ;; There is no install target (replace 'install (install-jars "."))))) (propagated-inputs `(("java-commons-codec" ,java-commons-codec) ("java-openchart2" ,java-openchart2))) (home-page "https://sites.google.com/site/cmzmasek/home/software/forester") (synopsis "Phylogenomics libraries for Java") (description "Forester is a collection of Java libraries for phylogenomics and evolutionary biology research. It includes support for reading, writing, and exporting phylogenetic trees.") (license license:lgpl2.1+)))) (define-public java-forester-1.005 (package (name "java-forester") (version "1.005") (source (origin (method url-fetch) (uri (string-append "https://repo1.maven.org/maven2/" "org/biojava/thirdparty/forester/" version "/forester-" version "-sources.jar")) (file-name (string-append name "-" version ".jar")) (sha256 (base32 "04r8qv4rk3p71z4ajrvp11py1z46qrx0047j3zzs79s6lnsm3lcv")))) (build-system ant-build-system) (arguments `(#:tests? #f ; there are none #:jdk ,icedtea-8 #:modules ((guix build ant-build-system) (guix build utils) (guix build java-utils) (sxml simple) (sxml transform)) #:phases (modify-phases %standard-phases (add-after 'unpack 'fix-dependencies (lambda* (#:key inputs #:allow-other-keys) (call-with-output-file "build.xml" (lambda (port) (sxml->xml (pre-post-order (with-input-from-file "src/build.xml" (lambda _ (xml->sxml #:trim-whitespace? #t))) `(;; Remove all unjar tags to avoid repacking classes. (unjar . ,(lambda _ '())) (*default* . ,(lambda (tag . kids) `(,tag ,@kids))) (*text* . ,(lambda (_ txt) txt)))) port))) (copy-file (assoc-ref inputs "synth_look_and_feel_1.xml") "synth_look_and_feel_1.xml") (copy-file (assoc-ref inputs "phyloxml.xsd") "phyloxml.xsd") (substitute* "build.xml" (("../resources/synth_laf/synth_look_and_feel_1.xml") "synth_look_and_feel_1.xml") (("../resources/phyloxml_schema/1.10/phyloxml.xsd") "phyloxml.xsd")) #t)) ;; FIXME: itext is difficult to package as it depends on a few ;; unpackaged libraries. (add-after 'unpack 'remove-dependency-on-unpackaged-itext (lambda _ (delete-file "src/org/forester/archaeopteryx/PdfExporter.java") (substitute* '("src/org/forester/archaeopteryx/MainFrame.java" "src/org/forester/archaeopteryx/MainFrameApplication.java") (("pdf_written_to = PdfExporter.*") "throw new IOException(\"PDF export is not available.\"); /*") ((".getPrintSizeX\\(\\), getOptions\\(\\).getPrintSizeY\\(\\) \\);") "*/") (("getCurrentTreePanel\\(\\).getHeight\\(\\) \\);") "*/")) #t)) (add-after 'unpack 'delete-pre-built-classes (lambda _ (delete-file-recursively "src/classes") #t)) ;; There is no install target (replace 'install (install-jars "."))))) (propagated-inputs `(("java-commons-codec" ,java-commons-codec) ("java-openchart2" ,java-openchart2))) ;; The source archive does not contain the resources. (native-inputs `(("phyloxml.xsd" ,(origin (method url-fetch) (uri (string-append "https://raw.githubusercontent.com/cmzmasek/forester/" "b61cc2dcede0bede317db362472333115756b8c6/" "forester/resources/phyloxml_schema/1.10/phyloxml.xsd")) (file-name (string-append name "-phyloxml-" version ".xsd")) (sha256 (base32 "1zxc4m8sn4n389nqdnpxa8d0k17qnr3pm2y5y6g6vh4k0zm52npv")))) ("synth_look_and_feel_1.xml" ,(origin (method url-fetch) (uri (string-append "https://raw.githubusercontent.com/cmzmasek/forester/" "29e04321615da6b35c1e15c60e52caf3f21d8e6a/" "forester/java/classes/resources/" "synth_look_and_feel_1.xml")) (file-name (string-append name "-synth-look-and-feel-" version ".xml")) (sha256 (base32 "1gv5602gv4k7y7713y75a4jvj7i9s7nildsbdl7n9q10sc2ikg8h")))))) (home-page "https://sites.google.com/site/cmzmasek/home/software/forester") (synopsis "Phylogenomics libraries for Java") (description "Forester is a collection of Java libraries for phylogenomics and evolutionary biology research. It includes support for reading, writing, and exporting phylogenetic trees.") (license license:lgpl2.1+))) (define-public java-biojava-core (package (name "java-biojava-core") (version "4.2.11") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/biojava/biojava") (commit (string-append "biojava-" version)))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "1bvryh2bpsvash8ln79cmc9sqm8qw72hz4xzwqxcrjm8ssxszhqk")))) (build-system ant-build-system) (arguments `(#:jdk ,icedtea-8 #:jar-name "biojava-core.jar" #:source-dir "biojava-core/src/main/java/" #:test-dir "biojava-core/src/test" ;; These tests seem to require internet access. #:test-exclude (list "**/SearchIOTest.java" "**/BlastXMLParserTest.java" "**/GenbankCookbookTest.java" "**/GenbankProxySequenceReaderTest.java") #:phases (modify-phases %standard-phases (add-before 'build 'copy-resources (lambda _ (copy-recursively "biojava-core/src/main/resources" "build/classes") #t)) (add-before 'check 'copy-test-resources (lambda _ (copy-recursively "biojava-core/src/test/resources" "build/test-classes") #t))))) (propagated-inputs `(("java-log4j-api" ,java-log4j-api) ("java-log4j-core" ,java-log4j-core) ("java-slf4j-api" ,java-slf4j-api) ("java-slf4j-simple" ,java-slf4j-simple))) (native-inputs `(("java-junit" ,java-junit) ("java-hamcrest-core" ,java-hamcrest-core))) (home-page "https://biojava.org") (synopsis "Core libraries of Java framework for processing biological data") (description "BioJava is a project dedicated to providing a Java framework for processing biological data. It provides analytical and statistical routines, parsers for common file formats, reference implementations of popular algorithms, and allows the manipulation of sequences and 3D structures. The goal of the biojava project is to facilitate rapid application development for bioinformatics. This package provides the core libraries.") (license license:lgpl2.1+))) (define-public java-biojava-phylo (package (inherit java-biojava-core) (name "java-biojava-phylo") (build-system ant-build-system) (arguments `(#:jdk ,icedtea-8 #:jar-name "biojava-phylo.jar" #:source-dir "biojava-phylo/src/main/java/" #:test-dir "biojava-phylo/src/test" #:phases (modify-phases %standard-phases (add-before 'build 'copy-resources (lambda _ (copy-recursively "biojava-phylo/src/main/resources" "build/classes") #t)) (add-before 'check 'copy-test-resources (lambda _ (copy-recursively "biojava-phylo/src/test/resources" "build/test-classes") #t))))) (propagated-inputs `(("java-log4j-api" ,java-log4j-api) ("java-log4j-core" ,java-log4j-core) ("java-slf4j-api" ,java-slf4j-api) ("java-slf4j-simple" ,java-slf4j-simple) ("java-biojava-core" ,java-biojava-core) ("java-forester" ,java-forester))) (native-inputs `(("java-junit" ,java-junit) ("java-hamcrest-core" ,java-hamcrest-core))) (home-page "https://biojava.org") (synopsis "Biojava interface to the forester phylogenomics library") (description "The phylo module provides a biojava interface layer to the forester phylogenomics library for constructing phylogenetic trees."))) (define-public java-biojava-alignment (package (inherit java-biojava-core) (name "java-biojava-alignment") (build-system ant-build-system) (arguments `(#:jdk ,icedtea-8 #:jar-name "biojava-alignment.jar" #:source-dir "biojava-alignment/src/main/java/" #:test-dir "biojava-alignment/src/test" #:phases (modify-phases %standard-phases (add-before 'build 'copy-resources (lambda _ (copy-recursively "biojava-alignment/src/main/resources" "build/classes") #t)) (add-before 'check 'copy-test-resources (lambda _ (copy-recursively "biojava-alignment/src/test/resources" "build/test-classes") #t))))) (propagated-inputs `(("java-log4j-api" ,java-log4j-api) ("java-log4j-core" ,java-log4j-core) ("java-slf4j-api" ,java-slf4j-api) ("java-slf4j-simple" ,java-slf4j-simple) ("java-biojava-core" ,java-biojava-core) ("java-biojava-phylo" ,java-biojava-phylo) ("java-forester" ,java-forester))) (native-inputs `(("java-junit" ,java-junit) ("java-hamcrest-core" ,java-hamcrest-core))) (home-page "https://biojava.org") (synopsis "Biojava API for genetic sequence alignment") (description "The alignment module of BioJava provides an API that contains @itemize @item implementations of dynamic programming algorithms for sequence alignment; @item reading and writing of popular alignment file formats; @item a single-, or multi- threaded multiple sequence alignment algorithm. @end itemize\n"))) (define-public java-biojava-core-4.0 (package (inherit java-biojava-core) (name "java-biojava-core") (version "4.0.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/biojava/biojava") (commit (string-append "biojava-" version)))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "13675f6y9aqi7bi2lk3s1z7a22ynccjiqwa8izh7p97xi9wsfmd8")))))) (define-public java-biojava-phylo-4.0 (package (inherit java-biojava-core-4.0) (name "java-biojava-phylo") (build-system ant-build-system) (arguments `(#:jdk ,icedtea-8 #:jar-name "biojava-phylo.jar" #:source-dir "biojava-phylo/src/main/java/" #:test-dir "biojava-phylo/src/test" #:phases (modify-phases %standard-phases (add-before 'build 'copy-resources (lambda _ (copy-recursively "biojava-phylo/src/main/resources" "build/classes") #t)) (add-before 'check 'copy-test-resources (lambda _ (copy-recursively "biojava-phylo/src/test/resources" "build/test-classes") #t))))) (propagated-inputs `(("java-log4j-api" ,java-log4j-api) ("java-log4j-core" ,java-log4j-core) ("java-slf4j-api" ,java-slf4j-api) ("java-slf4j-simple" ,java-slf4j-simple) ("java-biojava-core" ,java-biojava-core-4.0) ("java-forester" ,java-forester-1.005))) (native-inputs `(("java-junit" ,java-junit) ("java-hamcrest-core" ,java-hamcrest-core))) (home-page "https://biojava.org") (synopsis "Biojava interface to the forester phylogenomics library") (description "The phylo module provides a biojava interface layer to the forester phylogenomics library for constructing phylogenetic trees."))) (define-public java-biojava-alignment-4.0 (package (inherit java-biojava-core-4.0) (name "java-biojava-alignment") (build-system ant-build-system) (arguments `(#:jdk ,icedtea-8 #:jar-name "biojava-alignment.jar" #:source-dir "biojava-alignment/src/main/java/" #:test-dir "biojava-alignment/src/test" #:phases (modify-phases %standard-phases (add-before 'build 'copy-resources (lambda _ (copy-recursively "biojava-alignment/src/main/resources" "build/classes") #t)) (add-before 'check 'copy-test-resources (lambda _ (copy-recursively "biojava-alignment/src/test/resources" "build/test-classes") #t))))) (propagated-inputs `(("java-log4j-api" ,java-log4j-api) ("java-log4j-core" ,java-log4j-core) ("java-slf4j-api" ,java-slf4j-api) ("java-slf4j-simple" ,java-slf4j-simple) ("java-biojava-core" ,java-biojava-core-4.0) ("java-biojava-phylo" ,java-biojava-phylo-4.0) ("java-forester" ,java-forester-1.005))) (native-inputs `(("java-junit" ,java-junit) ("java-hamcrest-core" ,java-hamcrest-core))) (home-page "https://biojava.org") (synopsis "Biojava API for genetic sequence alignment") (description "The alignment module of BioJava provides an API that contains @itemize @item implementations of dynamic programming algorithms for sequence alignment; @item reading and writing of popular alignment file formats; @item a single-, or multi- threaded multiple sequence alignment algorithm. @end itemize\n"))) (define-public dropseq-tools (package (name "dropseq-tools") (version "1.13") (source (origin (method url-fetch) (uri "http://mccarrolllab.com/download/1276/") (file-name (string-append "dropseq-tools-" version ".zip")) (sha256 (base32 "0yrffckxqk5l8b5xb6z4laq157zd9mdypr2p4b4vq2bhjzi1sj0s")) ;; Delete bundled libraries (modules '((guix build utils))) (snippet '(begin (for-each delete-file (find-files "jar/lib" "\\.jar$")) (delete-file-recursively "3rdParty") #t)))) (build-system ant-build-system) (arguments `(#:tests? #f ; test data are not included #:test-target "test" #:build-target "all" #:source-dir "public/src/" #:jdk ,icedtea-8 #:make-flags (list (string-append "-Dpicard.executable.dir=" (assoc-ref %build-inputs "java-picard") "/share/java/")) #:modules ((ice-9 match) (srfi srfi-1) (guix build utils) (guix build java-utils) (guix build ant-build-system)) #:phases (modify-phases %standard-phases ;; FIXME: fails with "java.io.FileNotFoundException: ;; /gnu/store/…-dropseq-tools-1.13/share/java/lib/biojava-alignment.jar" (delete 'generate-jar-indices) ;; All dependencies must be linked to "lib", because that's where ;; they will be searched for when the Class-Path property of the ;; manifest is computed. (add-after 'unpack 'record-references (lambda* (#:key inputs #:allow-other-keys) (mkdir-p "jar/lib") (let ((dirs (filter-map (match-lambda ((name . dir) (if (and (string-prefix? "java-" name) (not (string=? name "java-testng"))) dir #f))) inputs))) (for-each (lambda (jar) (symlink jar (string-append "jar/lib/" (basename jar)))) (append-map (lambda (dir) (find-files dir "\\.jar$")) dirs))) #t)) ;; There is no installation target (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (share (string-append out "/share/java/")) (lib (string-append share "/lib/")) (scripts (list "BAMTagHistogram" "BAMTagofTagCounts" "BaseDistributionAtReadPosition" "CollapseBarcodesInPlace" "CollapseTagWithContext" "ConvertToRefFlat" "CreateIntervalsFiles" "DetectBeadSynthesisErrors" "DigitalExpression" "Drop-seq_alignment.sh" "FilterBAM" "FilterBAMByTag" "GatherGeneGCLength" "GatherMolecularBarcodeDistributionByGene" "GatherReadQualityMetrics" "PolyATrimmer" "ReduceGTF" "SelectCellsByNumTranscripts" "SingleCellRnaSeqMetricsCollector" "TagBamWithReadSequenceExtended" "TagReadWithGeneExon" "TagReadWithInterval" "TrimStartingSequence" "ValidateReference"))) (for-each mkdir-p (list bin share lib)) (install-file "dist/dropseq.jar" share) (for-each (lambda (script) (chmod script #o555) (install-file script bin)) scripts) (substitute* (map (lambda (script) (string-append bin "/" script)) scripts) (("^java") (which "java")) (("jar_deploy_dir=.*") (string-append "jar_deploy_dir=" share "\n")))) #t)) ;; FIXME: We do this after stripping jars because we don't want it to ;; copy all these jars and strip them. We only want to install ;; links. Arguably, this is a problem with the ant-build-system. (add-after 'strip-jar-timestamps 'install-links (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (share (string-append out "/share/java/")) (lib (string-append share "/lib/"))) (for-each (lambda (jar) (symlink (readlink jar) (string-append lib (basename jar)))) (find-files "jar/lib" "\\.jar$"))) #t))))) (inputs `(("jdk" ,icedtea-8) ("java-picard" ,java-picard-2.10.3) ("java-log4j-1.2-api" ,java-log4j-1.2-api) ("java-commons-math3" ,java-commons-math3) ("java-commons-jexl2" ,java-commons-jexl-2) ("java-commons-collections4" ,java-commons-collections4) ("java-commons-lang2" ,java-commons-lang) ("java-commons-io" ,java-commons-io) ("java-snappy-1.0.3-rc3" ,java-snappy-1) ("java-guava" ,java-guava) ("java-la4j" ,java-la4j) ("java-biojava-core" ,java-biojava-core-4.0) ("java-biojava-alignment" ,java-biojava-alignment-4.0) ("java-jdistlib" ,java-jdistlib) ("java-simple-xml" ,java-simple-xml) ("java-snakeyaml" ,java-snakeyaml))) (native-inputs `(("unzip" ,unzip) ("java-testng" ,java-testng))) (home-page "http://mccarrolllab.com/dropseq/") (synopsis "Tools for Drop-seq analyses") (description "Drop-seq is a technology to enable biologists to analyze RNA expression genome-wide in thousands of individual cells at once. This package provides tools to perform Drop-seq analyses.") (license license:expat))) (define-public pigx-rnaseq (package (name "pigx-rnaseq") (version "0.0.17") (source (origin (method url-fetch) (uri (string-append "https://github.com/BIMSBbioinfo/pigx_rnaseq/" "releases/download/v" version "/pigx_rnaseq-" version ".tar.gz")) (sha256 (base32 "0k9zj50bij3sjwq08v8l8waddcx8k66m3vdq8mx5vc23p19qz42s")))) (build-system gnu-build-system) (arguments `(#:parallel-tests? #f ; not supported #:phases (modify-phases %standard-phases ;; "test.sh" runs the whole pipeline, which takes a long time and ;; might fail due to OOM. The MultiQC is also resource intensive. (add-after 'unpack 'disable-resource-intensive-test (lambda _ (substitute* "Makefile.in" (("^ tests/test_multiqc/test.sh") "") (("^ test.sh") "")))) (add-before 'check 'set-timezone ;; The readr package is picky about timezones. (lambda* (#:key inputs #:allow-other-keys) (setenv "TZ" "UTC+1") (setenv "TZDIR" (string-append (assoc-ref inputs "tzdata") "/share/zoneinfo"))))))) (inputs `(("coreutils" ,coreutils) ("sed" ,sed) ("gzip" ,gzip) ("snakemake" ,snakemake) ("multiqc" ,multiqc) ("star" ,star-for-pigx) ("hisat2" ,hisat2) ("fastp" ,fastp) ("htseq" ,htseq) ("samtools" ,samtools) ("r-minimal" ,r-minimal) ("r-rmarkdown" ,r-rmarkdown) ("r-ggplot2" ,r-ggplot2) ("r-ggpubr" ,r-ggpubr) ("r-ggrepel" ,r-ggrepel) ("r-gprofiler2" ,r-gprofiler2) ("r-deseq2" ,r-deseq2) ("r-dt" ,r-dt) ("r-knitr" ,r-knitr) ("r-pheatmap" ,r-pheatmap) ("r-corrplot" ,r-corrplot) ("r-reshape2" ,r-reshape2) ("r-plotly" ,r-plotly) ("r-scales" ,r-scales) ("r-summarizedexperiment" ,r-summarizedexperiment) ("r-crosstalk" ,r-crosstalk) ("r-tximport" ,r-tximport) ("r-rtracklayer" ,r-rtracklayer) ("r-rjson" ,r-rjson) ("salmon" ,salmon) ("pandoc" ,pandoc) ("pandoc-citeproc" ,pandoc-citeproc) ("python-wrapper" ,python-wrapper) ("python-deeptools" ,python-deeptools) ("python-pyyaml" ,python-pyyaml))) (native-inputs `(("tzdata" ,tzdata))) (home-page "https://bioinformatics.mdc-berlin.de/pigx/") (synopsis "Analysis pipeline for RNA sequencing experiments") (description "PiGX RNAseq is an analysis pipeline for preprocessing and reporting for RNA sequencing experiments. It is easy to use and produces high quality reports. The inputs are reads files from the sequencing experiment, and a configuration file which describes the experiment. In addition to quality control of the experiment, the pipeline produces a differential expression report comparing samples in an easily configurable manner.") (license license:gpl3+))) (define-public pigx-chipseq (package (name "pigx-chipseq") (version "0.0.52") (source (origin (method url-fetch) (uri (string-append "https://github.com/BIMSBbioinfo/pigx_chipseq/" "releases/download/v" version "/pigx_chipseq-" version ".tar.gz")) (sha256 (base32 "097cvc8kr3r1nq0sgjpirzmixwjl074qp4qq3sx4ngfqi06af6r9")))) (build-system gnu-build-system) ;; parts of the tests rely on access to the network (arguments '(#:tests? #f)) (inputs `(("grep" ,grep) ("coreutils" ,coreutils) ("r-minimal" ,r-minimal) ("r-argparser" ,r-argparser) ("r-biocparallel" ,r-biocparallel) ("r-biostrings" ,r-biostrings) ("r-chipseq" ,r-chipseq) ("r-corrplot" ,r-corrplot) ("r-data-table" ,r-data-table) ("r-deseq2" ,r-deseq2) ("r-dplyr" ,r-dplyr) ("r-dt" ,r-dt) ("r-genomation" ,r-genomation) ("r-genomicalignments" ,r-genomicalignments) ("r-genomicranges" ,r-genomicranges) ("r-ggplot2" ,r-ggplot2) ("r-ggrepel" ,r-ggrepel) ("r-gprofiler2" ,r-gprofiler2) ("r-heatmaply" ,r-heatmaply) ("r-htmlwidgets" ,r-htmlwidgets) ("r-jsonlite" ,r-jsonlite) ("r-pheatmap" ,r-pheatmap) ("r-plotly" ,r-plotly) ("r-rmarkdown" ,r-rmarkdown) ("r-rsamtools" ,r-rsamtools) ("r-rsubread" ,r-rsubread) ("r-rtracklayer" ,r-rtracklayer) ("r-s4vectors" ,r-s4vectors) ("r-stringr" ,r-stringr) ("r-tibble" ,r-tibble) ("r-tidyr" ,r-tidyr) ("python-wrapper" ,python-wrapper) ("python-pyyaml" ,python-pyyaml) ("python-magic" ,python-magic) ("python-xlrd" ,python-xlrd) ("trim-galore" ,trim-galore) ("macs" ,macs) ("multiqc" ,multiqc) ("perl" ,perl) ("pandoc" ,pandoc) ("pandoc-citeproc" ,pandoc-citeproc) ("fastqc" ,fastqc) ("bowtie" ,bowtie) ("idr" ,idr) ("snakemake" ,snakemake) ("samtools" ,samtools) ("bedtools" ,bedtools) ("kentutils" ,kentutils))) (native-inputs `(("python-pytest" ,python-pytest))) (home-page "https://bioinformatics.mdc-berlin.de/pigx/") (synopsis "Analysis pipeline for ChIP sequencing experiments") (description "PiGX ChIPseq is an analysis pipeline for preprocessing, peak calling and reporting for ChIP sequencing experiments. It is easy to use and produces high quality reports. The inputs are reads files from the sequencing experiment, and a configuration file which describes the experiment. In addition to quality control of the experiment, the pipeline enables to set up multiple peak calling analysis and allows the generation of a UCSC track hub in an easily configurable manner.") (license license:gpl3+))) (define-public pigx-bsseq (package (name "pigx-bsseq") (version "0.1.5") (source (origin (method url-fetch) (uri (string-append "https://github.com/BIMSBbioinfo/pigx_bsseq/" "releases/download/v" version "/pigx_bsseq-" version ".tar.gz")) (sha256 (base32 "05al5dacfp1vf1x3cq20jhd6w4xj5vaxslzaka6yrpg0av8sh3k3")))) (build-system gnu-build-system) (arguments `(;; TODO: tests currently require 12+GB of RAM. See ;; https://github.com/BIMSBbioinfo/pigx_bsseq/issues/164 #:tests? #f #:phases (modify-phases %standard-phases (add-before 'check 'set-timezone ;; The readr package is picky about timezones. (lambda* (#:key inputs #:allow-other-keys) (setenv "TZ" "UTC+1") (setenv "TZDIR" (string-append (assoc-ref inputs "tzdata") "/share/zoneinfo"))))))) (native-inputs `(("tzdata" ,tzdata))) (inputs `(("coreutils" ,coreutils) ("sed" ,sed) ("grep" ,grep) ("r-minimal" ,r-minimal) ("r-annotationhub" ,r-annotationhub) ("r-dt" ,r-dt) ("r-genomation" ,r-genomation) ("r-ggbio" ,r-ggbio) ("r-ggrepel" ,r-ggrepel) ("r-matrixstats" ,r-matrixstats) ("r-methylkit" ,r-methylkit) ("r-reshape2" ,r-reshape2) ("r-rtracklayer" ,r-rtracklayer) ("r-rmarkdown" ,r-rmarkdown) ("r-bookdown" ,r-bookdown) ("r-ggplot2" ,r-ggplot2) ("r-ggbio" ,r-ggbio) ("pandoc" ,pandoc) ("pandoc-citeproc" ,pandoc-citeproc) ("python-wrapper" ,python-wrapper) ("python-pyyaml" ,python-pyyaml) ("snakemake" ,snakemake) ("bismark" ,bismark) ("bowtie" ,bowtie) ("bwa-meth" ,bwa-meth) ("fastqc" ,fastqc) ("methyldackel" ,methyldackel) ("multiqc" ,multiqc) ("trim-galore" ,trim-galore) ("cutadapt" ,cutadapt) ("samblaster" ,samblaster) ("samtools" ,samtools))) (home-page "https://bioinformatics.mdc-berlin.de/pigx/") (synopsis "Bisulfite sequencing pipeline from fastq to methylation reports") (description "PiGx BSseq is a data processing pipeline for raw fastq read data of bisulfite experiments; it produces reports on aggregate methylation and coverage and can be used to produce information on differential methylation and segmentation.") (license license:gpl3+))) (define-public pigx-scrnaseq (package (name "pigx-scrnaseq") (version "1.1.7") (source (origin (method url-fetch) (uri (string-append "https://github.com/BIMSBbioinfo/pigx_scrnaseq/" "releases/download/v" version "/pigx_scrnaseq-" version ".tar.gz")) (sha256 (base32 "1h5mcxzwj3cidlkvy9ly5wmi48vwfsjf8dxjfirknqxr9a92hwlx")))) (build-system gnu-build-system) (inputs `(("coreutils" ,coreutils) ("perl" ,perl) ("fastqc" ,fastqc) ("flexbar" ,flexbar) ("java" ,icedtea-8) ("jellyfish" ,jellyfish) ("python-wrapper" ,python-wrapper) ("python-pyyaml" ,python-pyyaml) ("python-pandas" ,python-pandas) ("python-magic" ,python-magic) ("python-numpy" ,python-numpy) ("python-loompy" ,python-loompy) ("pandoc" ,pandoc) ("pandoc-citeproc" ,pandoc-citeproc) ("samtools" ,samtools) ("snakemake" ,snakemake) ("star" ,star-for-pigx) ("r-minimal" ,r-minimal) ("r-argparser" ,r-argparser) ("r-cowplot" ,r-cowplot) ("r-data-table" ,r-data-table) ("r-delayedarray" ,r-delayedarray) ("r-delayedmatrixstats" ,r-delayedmatrixstats) ("r-dplyr" ,r-dplyr) ("r-dropbead" ,r-dropbead) ("r-dt" ,r-dt) ("r-genomicalignments" ,r-genomicalignments) ("r-genomicfiles" ,r-genomicfiles) ("r-genomicranges" ,r-genomicranges) ("r-ggplot2" ,r-ggplot2) ("r-hdf5array" ,r-hdf5array) ("r-pheatmap" ,r-pheatmap) ("r-rmarkdown" ,r-rmarkdown) ("r-rsamtools" ,r-rsamtools) ("r-rtracklayer" ,r-rtracklayer) ("r-rtsne" ,r-rtsne) ("r-scater" ,r-scater) ("r-scran" ,r-scran) ("r-seurat" ,r-seurat) ("r-singlecellexperiment" ,r-singlecellexperiment) ("r-stringr" ,r-stringr) ("r-yaml" ,r-yaml))) (home-page "https://bioinformatics.mdc-berlin.de/pigx/") (synopsis "Analysis pipeline for single-cell RNA sequencing experiments") (description "PiGX scRNAseq is an analysis pipeline for preprocessing and quality control for single cell RNA sequencing experiments. The inputs are read files from the sequencing experiment, and a configuration file which describes the experiment. It produces processed files for downstream analysis and interactive quality reports. The pipeline is designed to work with UMI based methods.") (license license:gpl3+))) (define-public pigx-sars-cov2-ww (package (name "pigx-sars-cov2-ww") (version "0.0.2") (source (origin (method url-fetch) (uri (string-append "https://github.com/BIMSBbioinfo/pigx_sarscov2_ww/" "releases/download/v" version "/pigx_sars-cov2-ww-" version ".tar.gz")) (sha256 (base32 "0yrbza7lbzijjc8ifk06b646n959a4k7id94x3ndz795892f64l0")))) (build-system gnu-build-system) (inputs `(("bash-minimal" ,bash-minimal) ("bwa" ,bwa) ("ensembl-vep" ,ensembl-vep) ("fastqc" ,fastqc) ("kraken2" ,kraken2) ("krona-tools" ,krona-tools) ("lofreq" ,lofreq) ("multiqc" ,multiqc) ("prinseq" ,prinseq) ("python-pyyaml" ,python-pyyaml) ("python-wrapper" ,python-wrapper) ("r-base64url" ,r-base64url) ("r-dplyr" ,r-dplyr) ("r-dt" ,r-dt) ("r-ggplot2" ,r-ggplot2) ("r-magrittr" ,r-magrittr) ("r-minimal" ,r-minimal) ("r-plotly" ,r-plotly) ("r-qpcr" ,r-qpcr) ("r-reshape2" ,r-reshape2) ("r-rmarkdown" ,r-rmarkdown) ("r-stringr" ,r-stringr) ("r-tidyr" ,r-tidyr) ("samtools" ,samtools) ("snakemake" ,snakemake) ("wget" ,wget))) (home-page "https://bioinformatics.mdc-berlin.de/pigx/") (synopsis "Analysis pipeline for wastewater sequencing") (description "PiGx SARS-CoV-2 is a pipeline for analysing data from sequenced wastewater samples and identifying given variants-of-concern of SARS-CoV-2. The pipeline can be used for continuous sampling. The output report will provide an intuitive visual overview about the development of variant abundance over time and location.") (license license:gpl3+))) (define-public pigx (package (name "pigx") (version "0.0.3") (source (origin (method url-fetch) (uri (string-append "https://github.com/BIMSBbioinfo/pigx/" "releases/download/v" version "/pigx-" version ".tar.gz")) (sha256 (base32 "1i5njdy1clj5ncw45d16p7mwmqvb1ilikl9n797pxklc3f4s7mq7")))) (build-system gnu-build-system) (inputs `(("python" ,python) ("pigx-bsseq" ,pigx-bsseq) ("pigx-chipseq" ,pigx-chipseq) ("pigx-rnaseq" ,pigx-rnaseq) ("pigx-scrnaseq" ,pigx-scrnaseq))) (home-page "https://bioinformatics.mdc-berlin.de/pigx/") (synopsis "Analysis pipelines for genomics") (description "PiGx is a collection of genomics pipelines. It includes the following pipelines: @itemize @item PiGx BSseq for raw fastq read data of bisulfite experiments @item PiGx RNAseq for RNAseq samples @item PiGx scRNAseq for single cell dropseq analysis @item PiGx ChIPseq for reads from ChIPseq experiments @end itemize All pipelines are easily configured with a simple sample sheet and a descriptive settings file. The result is a set of comprehensive, interactive HTML reports with interesting findings about your samples.") (license license:gpl3+))) (define-public genrich (package (name "genrich") (version "0.5") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/jsh58/Genrich") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0x0q6z0208n3cxzqjla4rgjqpyqgwpmz27852lcvzkzaigymq4zp")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are none #:phases (modify-phases %standard-phases (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (install-file "Genrich" (string-append (assoc-ref outputs "out") "/bin")) #t))))) (inputs `(("zlib" ,zlib))) (home-page "https://github.com/jsh58/Genrich") (synopsis "Detecting sites of genomic enrichment") (description "Genrich is a peak-caller for genomic enrichment assays (e.g. ChIP-seq, ATAC-seq). It analyzes alignment files generated following the assay and produces a file detailing peaks of significant enrichment.") (license license:expat))) (define-public mantis (let ((commit "4ffd171632c2cb0056a86d709dfd2bf21bc69b84") (revision "1")) (package (name "mantis") (version (git-version "0" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/splatlab/mantis") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "0iqbr0dhmlc8mzpirmm2s4pkzkwdgrcx50yx6cv3wlr2qi064p55")))) (build-system cmake-build-system) (arguments '(#:tests? #f)) ; there are none (inputs `(("sdsl-lite" ,sdsl-lite) ("openssl" ,openssl) ("zlib" ,zlib))) (home-page "https://github.com/splatlab/mantis") (synopsis "Large-scale sequence-search index data structure") (description "Mantis is a space-efficient data structure that can be used to index thousands of raw-read genomics experiments and facilitate large-scale sequence searches on those experiments. Mantis uses counting quotient filters instead of Bloom filters, enabling rapid index builds and queries, small indexes, and exact results, i.e., no false positives or negatives. Furthermore, Mantis is also a colored de Bruijn graph representation, so it supports fast graph traversal and other topological analyses in addition to large-scale sequence-level searches.") ;; uses __uint128_t and inline assembly (supported-systems '("x86_64-linux")) (license license:bsd-3)))) (define-public sjcount ;; There is no tag for version 3.2, nor is there a release archive. (let ((commit "292d3917cadb3f6834c81e509c30e61cd7ead6e5") (revision "1")) (package (name "sjcount") (version (git-version "3.2" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/pervouchine/sjcount-full") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "0gdgj35j249f04rqgq8ymcc1xg1vi9kzbajnjqpaq2wpbh8bl234")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; requires a 1.4G test file #:make-flags (list (string-append "SAMTOOLS_DIR=" (assoc-ref %build-inputs "samtools") "/lib/")) #:phases (modify-phases %standard-phases (replace 'configure (lambda* (#:key inputs #:allow-other-keys) (substitute* "makefile" (("-I \\$\\{SAMTOOLS_DIR\\}") (string-append "-I" (assoc-ref inputs "samtools") "/include/samtools")) (("-lz ") "-lz -lpthread ")) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (for-each (lambda (tool) (install-file tool (string-append (assoc-ref outputs "out") "/bin"))) '("j_count" "b_count" "sjcount")) #t))))) (inputs `(("samtools" ,samtools-0.1) ("zlib" ,zlib))) (home-page "https://github.com/pervouchine/sjcount-full/") (synopsis "Annotation-agnostic splice junction counting pipeline") (description "Sjcount is a utility for fast quantification of splice junctions in RNA-seq data. It is annotation-agnostic and offset-aware. This version does count multisplits.") (license license:gpl3+)))) (define-public minimap2 (package (name "minimap2") (version "2.18") (source (origin (method url-fetch) (uri (string-append "https://github.com/lh3/minimap2/" "releases/download/v" version "/" "minimap2-" version ".tar.bz2")) (sha256 (base32 "1d7fvdqcqd6wns875rkyd7f34ii15gc9l1sivd2wbbpcb0fi0mbs")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are none #:modules ((guix build utils) (guix build gnu-build-system) (srfi srfi-26)) #:make-flags (list (string-append "CC=" ,(cc-for-target)) (let ((system ,(or (%current-target-system) (%current-system)))) (cond ((string-prefix? "x86_64" system) "all") ((or (string-prefix? "i586" system) (string-prefix? "i686" system)) "sse2only=1") ((string-prefix? "armhf" system) "arm_neon=1") ((string-prefix? "aarch64" system) "aarch64=1") (else "")))) #:phases (modify-phases %standard-phases (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (lib (string-append out "/lib")) (inc (string-append out "/include")) (man (string-append out "/share/man/man1"))) (install-file "minimap2" bin) (install-file "libminimap2.a" lib) (install-file "minimap2.1" man) (map (cut install-file <> inc) (find-files "." "\\.h$")) ;; Not this file. (delete-file (string-append inc "/emmintrin.h")) (mkdir-p (string-append lib "/pkgconfig")) (with-output-to-file (string-append lib "/pkgconfig/minimap2.pc") (lambda _ (format #t "prefix=~a~@ exec_prefix=${prefix}~@ libdir=${exec_prefix}/lib~@ includedir=${prefix}/include~@ ~@ Name: libminimap2~@ Version: ~a~@ Description: A versatile pairwise aligner for genomic and spliced nucleotide sequence~@ Libs: -L${libdir} -lminimap2~@ Cflags: -I${includedir}~%" out ,version)))) #t))))) (inputs `(("zlib" ,zlib))) (home-page "https://lh3.github.io/minimap2/") (synopsis "Pairwise aligner for genomic and spliced nucleotide sequences") (description "Minimap2 is a versatile sequence alignment program that aligns DNA or mRNA sequences against a large reference database. Typical use cases include: @enumerate @item mapping PacBio or Oxford Nanopore genomic reads to the human genome; @item finding overlaps between long reads with error rate up to ~15%; @item splice-aware alignment of PacBio Iso-Seq or Nanopore cDNA or Direct RNA reads against a reference genome; @item aligning Illumina single- or paired-end reads; @item assembly-to-assembly alignment; @item full-genome alignment between two closely related species with divergence below ~15%. @end enumerate\n") (license license:expat))) (define-public python-mappy (package (name "python-mappy") (version "2.18") (source (origin (method url-fetch) (uri (pypi-uri "mappy" version)) (sha256 (base32 "1a05p7rkmxa6qhm108na8flzj2v45jab06drk59kzk1ip2sgvzqq")))) (build-system python-build-system) (native-inputs `(("python-cython" ,python-cython))) (inputs `(("zlib" ,zlib))) (home-page "https://github.com/lh3/minimap2") (synopsis "Python binding for minimap2") (description "This package provides a convenient interface to minimap2, a fast and accurate C program to align genomic and transcribe nucleotide sequences.") (license license:expat))) (define-public miniasm (package (name "miniasm") (version "0.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/lh3/miniasm") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "04dv5wv8bhsw1imxwyd438bnn9kby7svp44nbcz8lsadzjjci5gs")))) (build-system gnu-build-system) (inputs `(("zlib" ,zlib))) (arguments `(#:tests? #f ; There are no tests. #:phases (modify-phases %standard-phases (delete 'configure) (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (install-file "miniasm" bin) (install-file "minidot" bin) #t)))))) (home-page "https://github.com/lh3/miniasm") (synopsis "Ultrafast de novo assembly for long noisy reads") (description "Miniasm is a very fast OLC-based de novo assembler for noisy long reads. It takes all-vs-all read self-mappings (typically by minimap) as input and outputs an assembly graph in the GFA format. Different from mainstream assemblers, miniasm does not have a consensus step. It simply concatenates pieces of read sequences to generate the final unitig sequences. Thus the per-base error rate is similar to the raw input reads.") (license license:expat))) (define-public bandage (package (name "bandage") (version "0.8.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/rrwick/Bandage") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1bbsn5f5x8wlspg4pbibqz6m5vin8c19nl224f3z3km0pkc97rwv")))) (build-system qt-build-system) (arguments `(#:phases (modify-phases %standard-phases (replace 'configure (lambda _ (invoke "qmake" "Bandage.pro"))) (replace 'check (lambda* (#:key tests? #:allow-other-keys) (when tests? (substitute* "tests/bandage_command_line_tests.sh" (("^bandagepath=.*") (string-append "bandagepath=" (getcwd) "/Bandage\n"))) (with-directory-excursion "tests" (setenv "XDG_RUNTIME_DIR" (getcwd)) (invoke "./bandage_command_line_tests.sh"))) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) (install-file "Bandage" (string-append out "/bin")) #t)))))) (inputs `(("qtbase" ,qtbase-5) ("qtsvg" ,qtsvg))) (native-inputs `(("imagemagick" ,imagemagick))) (home-page "https://rrwick.github.io/Bandage/") (synopsis "Bioinformatics Application for Navigating De novo Assembly Graphs Easily") (description "Bandage is a program for visualising de novo assembly graphs. It allows users to interact with the assembly graphs made by de novo assemblers such as Velvet, SPAdes, MEGAHIT and others. De novo assembly graphs contain not only assembled contigs but also the connections between those contigs, which were previously not easily accessible. Bandage visualises assembly graphs, with connections, using graph layout algorithms. Nodes in the drawn graph, which represent contigs, can be automatically labelled with their ID, length or depth. Users can interact with the graph by moving, labelling and colouring nodes. Sequence information can also be extracted directly from the graph viewer. By displaying connections between contigs, Bandage opens up new possibilities for analysing and improving de novo assemblies that are not possible by looking at contigs alone.") (license (list license:gpl2+ ; bundled ogdf license:gpl3+)))) (define-public libmaus2 (package (name "libmaus2") (version "2.0.786") (source (origin (method git-fetch) (uri (git-reference (url "https://gitlab.com/german.tischler/libmaus2") (commit (string-append version "-release-20210531143054")))) (file-name (git-file-name name version)) (sha256 (base32 "1rxakmwjcx2yq5sjh3v849f7dfw4xzc2fyzf6s28s3p95z84w564")))) (build-system gnu-build-system) ;; The test suite attempts to execute ../test-driver, which does not exist. (arguments '(#:tests? #false)) (propagated-inputs `(("zlib" ,zlib))) (native-inputs `(("gcc" ,gcc-10) ;Code has C++17 requirements ("pkg-config" ,pkg-config))) (home-page "https://gitlab.com/german.tischler/libmaus2") (synopsis "Collection of data structures and algorithms useful for bioinformatics") (description "libmaus2 is a collection of data structures and algorithms. It contains: @itemize @item I/O classes (single byte and UTF-8); @item @code{bitio} classes (input, output and various forms of bit level manipulation); @item text indexing classes (suffix and LCP array, fulltext and minute (FM), etc.); @item BAM sequence alignment files input/output (simple and collating); and many lower level support classes. @end itemize\n") ;; The code is explicitly available under the terms of either GPLv2 or ;; GPLv3 according to the AUTHORS file, though most files have a GPLv3+ ;; license header. (license (list license:gpl2+ license:gpl3+)))) (define-public biobambam2 (package (name "biobambam2") (version "2.0.182") (source (origin (method git-fetch) (uri (git-reference (url "https://gitlab.com/german.tischler/biobambam2") (commit (string-append version "-release-20210412001032")))) (file-name (git-file-name name version)) (sha256 (base32 "0b7w7a2a7hpkgrdn0n7hy4pilzrj82zqrh7q4bg1l0cd6bqr60m5")))) (build-system gnu-build-system) (arguments ;; The test suite attempts to execute ../test-driver, which does not exist. `(#:tests? #false #:configure-flags (list (string-append "--with-libmaus2=" (assoc-ref %build-inputs "libmaus2"))))) (inputs `(("libmaus2" ,libmaus2) ("xerces-c" ,xerces-c))) (native-inputs `(("gcc" ,gcc-10) ;Code has C++17 requirements ("pkg-config" ,pkg-config))) (home-page "https://gitlab.com/german.tischler/biobambam2") (synopsis "Tools for processing BAM files") (description "This package contains some tools for processing BAM files including: @itemize @item bamsormadup: parallel sorting and duplicate marking @item bamcollate2: reads BAM and writes BAM reordered such that alignment or collated by query name @item bammarkduplicates: reads BAM and writes BAM with duplicate alignments marked using the BAM flags field @item bammaskflags: reads BAM and writes BAM while masking (removing) bits from the flags column @item bamrecompress: reads BAM and writes BAM with a defined compression setting. This tool is capable of multi-threading. @item bamsort: reads BAM and writes BAM resorted by coordinates or query name @item bamtofastq: reads BAM and writes FastQ; output can be collated or uncollated by query name. @end itemize ") ;; The COPYING file states that the code is distributed under version 3 of ;; the GPL, but the license headers include the "or later" clause. (license license:gpl3+))) (define-public r-circus (package (name "r-circus") (version "0.1.5") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/BIMSBbioinfo/ciRcus") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0jhjn3ilb057hbf6yzrihj13ifxxs32y7nkby8l3lkm28dg4p97h")))) (build-system r-build-system) (propagated-inputs `(("r-annotationdbi" ,r-annotationdbi) ("r-annotationhub" ,r-annotationhub) ("r-biomart" ,r-biomart) ("r-data-table" ,r-data-table) ("r-dbi" ,r-dbi) ("r-genomicfeatures" ,r-genomicfeatures) ("r-genomicranges" ,r-genomicranges) ("r-ggplot2" ,r-ggplot2) ("r-hash" ,r-hash) ("r-iranges" ,r-iranges) ("r-rcolorbrewer" ,r-rcolorbrewer) ("r-rmysql" ,r-rmysql) ("r-s4vectors" ,r-s4vectors) ("r-stringr" ,r-stringr) ("r-summarizedexperiment" ,r-summarizedexperiment))) (native-inputs `(("r-knitr" ,r-knitr))) (home-page "https://github.com/BIMSBbioinfo/ciRcus") (synopsis "Annotation, analysis and visualization of circRNA data") (description "Circus is an R package for annotation, analysis and visualization of circRNA data. Users can annotate their circRNA candidates with host genes, gene featrues they are spliced from, and discriminate between known and yet unknown splice junctions. Circular-to-linear ratios of circRNAs can be calculated, and a number of descriptive plots easily generated.") (license license:artistic2.0))) (define-public gffread ;; We cannot use the tagged release because it is not in sync with gclib. ;; See https://github.com/gpertea/gffread/issues/26 (let ((commit "ba7535fcb3cea55a6e5a491d916e93b454e87fd0") (revision "1")) (package (name "gffread") (version (git-version "0.9.12" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/gpertea/gffread") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "1dl2nbcg96lxpd0drg48ssa8343nf7pw9s9mkrc4mjjmfwsin3ki")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no check target #:make-flags (list "GCLDIR=gclib") #:phases (modify-phases %standard-phases (delete 'configure) (add-after 'unpack 'copy-gclib-source (lambda* (#:key inputs #:allow-other-keys) (mkdir-p "gclib") (copy-recursively (assoc-ref inputs "gclib-source") "gclib") #t)) ;; There is no install target (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin"))) (install-file "gffread" bin)) #t))))) (native-inputs `(("gclib-source" ,(let ((version "0.10.3") (commit "54917d0849c1e83cfb057b5f712e5cb6a35d948f") (revision "1")) (origin (method git-fetch) (uri (git-reference (url "https://github.com/gpertea/gclib") (commit commit))) (file-name (git-file-name "gclib" version)) (sha256 (base32 "0b51lc0b8syrv7186fd7n8f15rwnf264qgfmm2palrwks1px24mr"))))))) (home-page "https://github.com/gpertea/gffread/") (synopsis "Parse and convert GFF/GTF files") (description "This package provides a GFF/GTF file parsing utility providing format conversions, region filtering, FASTA sequence extraction and more.") ;; gffread is under Expat, but gclib is under Artistic 2.0 (license (list license:expat license:artistic2.0))))) (define-public find-circ ;; The last release was in 2015. The license was clarified in 2017, so we ;; take the latest commit. (let ((commit "8655dca54970fcf7e92e22fbf57e1188724dda7d") (revision "1")) (package (name "find-circ") (version (git-version "1.2" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/marvin-jens/find_circ") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "0p77pbqbclqr4srms34y1b9b4njybfpjiknc11ki84f3p8skb3cg")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are none #:phases ;; There is no actual build system. (modify-phases %standard-phases (delete 'configure) (delete 'build) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (path (getenv "PYTHONPATH"))) (for-each (lambda (script) (install-file script bin) (wrap-program (string-append bin "/" script) `("PYTHONPATH" ":" prefix (,path)))) '("cmp_bed.py" "find_circ.py" "maxlength.py" "merge_bed.py" "unmapped2anchors.py"))) #t))))) (inputs `(("python2" ,python-2) ("python2-pysam" ,python2-pysam) ("python2-numpy" ,python2-numpy))) (home-page "https://github.com/marvin-jens/find_circ") (synopsis "circRNA detection from RNA-seq reads") (description "This package provides tools to detect head-to-tail spliced (back-spliced) sequencing reads, indicative of circular RNA (circRNA) in RNA-seq data.") (license license:gpl3)))) (define-public fit-sne (package (name "fit-sne") (version "1.2.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/KlugerLab/FIt-SNE") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1imq4577awc226wvygf94kpz156qdfw8xl0w0f7ss4w10lhmpmf5")))) (build-system gnu-build-system) (arguments `(#:tests? #false ; there are none #:phases ;; There is no build system. (modify-phases %standard-phases (delete 'configure) (replace 'build (lambda _ (invoke "g++" "-std=c++11" "-O3" "src/sptree.cpp" "src/tsne.cpp" "src/nbodyfft.cpp" "-o" "bin/fast_tsne" "-pthread" "-lfftw3" "-lm" "-Wno-address-of-packed-member"))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (share (string-append out "/share/fit-sne"))) (for-each (lambda (file) (install-file file bin)) (find-files "bin")) (substitute* "fast_tsne.R" (("^FAST_TSNE_SCRIPT_DIR.*") (string-append "FAST_TSNE_SCRIPT_DIR = \"" out "\"\n"))) (install-file "fast_tsne.R" share))))))) (inputs `(("fftw" ,fftw))) (home-page "https://github.com/KlugerLab/FIt-SNE") (synopsis "Fast Fourier Transform-accelerated interpolation-based t-SNE") (description "@dfn{t-Stochastic Neighborhood Embedding} (t-SNE) is a method for dimensionality reduction and visualization of high dimensional datasets. A popular implementation of t-SNE uses the Barnes-Hut algorithm to approximate the gradient at each iteration of gradient descent. This implementation differs in these ways: @itemize @item Instead of approximating the N-body simulation using Barnes-Hut, we interpolate onto an equispaced grid and use FFT to perform the convolution. @item Instead of computing nearest neighbors using vantage-point trees, we approximate nearest neighbors using the Annoy library. The neighbor lookups are multithreaded to take advantage of machines with multiple cores. @end itemize ") ;; See LICENSE.txt for details on what license applies to what files. (license (list license:bsd-4 license:expat license:asl2.0)))) (define-public python-scanpy (package (name "python-scanpy") (version "1.7.2") (source (origin (method url-fetch) (uri (pypi-uri "scanpy" version)) (sha256 (base32 "0c66adnfizsyk0h8bv2yhmay876z0klpxwpn4z6m71wly7yplpmd")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (replace 'check (lambda* (#:key inputs #:allow-other-keys) ;; These tests require Internet access. (delete-file-recursively "scanpy/tests/notebooks") (delete-file "scanpy/tests/test_clustering.py") (delete-file "scanpy/tests/test_datasets.py") (delete-file "scanpy/tests/test_highly_variable_genes.py") ;; TODO: I can't get the plotting tests to work, even with Xvfb. (delete-file "scanpy/tests/test_plotting.py") (delete-file "scanpy/tests/test_preprocessing.py") (delete-file "scanpy/tests/test_read_10x.py") ;; The following tests need anndata.tests, which aren't included ;; in the final python-anndata package. (delete-file "scanpy/tests/test_combat.py") (delete-file "scanpy/tests/test_embedding_plots.py") (delete-file "scanpy/tests/test_normalization.py") (delete-file "scanpy/tests/test_pca.py") (delete-file "scanpy/tests/external/test_scrublet.py") ;; The following tests requires 'scanorama', which isn't ;; packaged yet. (delete-file "scanpy/tests/external/test_scanorama_integrate.py") (setenv "PYTHONPATH" (string-append (getcwd) ":" (getenv "PYTHONPATH"))) (invoke "pytest") #t))))) (propagated-inputs `(("python-anndata" ,python-anndata) ("python-h5py" ,python-h5py) ("python-igraph" ,python-igraph) ("python-joblib" ,python-joblib) ("python-legacy-api-wrap" ,python-legacy-api-wrap) ("python-louvain" ,python-louvain-0.6) ("python-matplotlib" ,python-matplotlib) ("python-natsort" ,python-natsort) ("python-networkx" ,python-networkx) ("python-numba" ,python-numba) ("python-packaging" ,python-packaging) ("python-pandas" ,python-pandas) ("python-patsy" ,python-patsy) ("python-scikit-learn" ,python-scikit-learn) ("python-scipy" ,python-scipy) ("python-seaborn" ,python-seaborn) ("python-statsmodels" ,python-statsmodels) ("python-tables" ,python-tables) ("python-pytoml" ,python-pytoml) ("python-tqdm" ,python-tqdm) ("python-umap-learn" ,python-umap-learn))) (native-inputs `(("python-leidenalg" ,python-leidenalg) ("python-pytest" ,python-pytest) ("python-setuptools-scm" ,python-setuptools-scm) ("python-sinfo" ,python-sinfo))) (home-page "https://github.com/theislab/scanpy") (synopsis "Single-Cell Analysis in Python.") (description "Scanpy is a scalable toolkit for analyzing single-cell gene expression data. It includes preprocessing, visualization, clustering, pseudotime and trajectory inference and differential expression testing. The Python-based implementation efficiently deals with datasets of more than one million cells.") (license license:bsd-3))) (define-public python-bbknn (package (name "python-bbknn") (version "1.3.6") (source (origin (method url-fetch) (uri (pypi-uri "bbknn" version)) (sha256 (base32 "1jbsh01f57zj4bhvjr3jh4532zznqd6nccmgrl3qi9gnhkf7c4y0")))) (build-system python-build-system) (arguments `(#:tests? #f)) ; TODO: Enable after migration to scikit-learn. (propagated-inputs `(("python-annoy" ,python-annoy) ("python-cython" ,python-cython) ("python-numpy" ,python-numpy) ("python-scipy" ,python-scipy) ("python-umap-learn" ,python-umap-learn))) (home-page "https://github.com/Teichlab/bbknn") (synopsis "Batch balanced KNN") (description "BBKNN is a batch effect removal tool that can be directly used in the Scanpy workflow. It serves as an alternative to @code{scanpy.api.pp.neighbors()}, with both functions creating a neighbour graph for subsequent use in clustering, pseudotime and UMAP visualisation. If technical artifacts are present in the data, they will make it challenging to link corresponding cell types across different batches. BBKNN actively combats this effect by splitting your data into batches and finding a smaller number of neighbours for each cell within each of the groups. This helps create connections between analogous cells in different batches without altering the counts or PCA space.") (license license:expat))) (define-public python-drep (package (name "python-drep") (version "3.2.0") (source (origin (method url-fetch) (uri (pypi-uri "drep" version)) (sha256 (base32 "08vk0x6v5c5n7afgd5pcjhsvb424absypxy22hw1cm1n9kirbi77")))) (build-system python-build-system) (propagated-inputs `(("python-biopython" ,python-biopython) ("python-matplotlib" ,python-matplotlib) ("python-numpy" ,python-numpy) ("python-pandas" ,python-pandas) ("python-pytest" ,python-pytest) ("python-scikit-learn" ,python-scikit-learn) ("python-seaborn" ,python-seaborn) ("python-tqdm" ,python-tqdm))) (home-page "https://github.com/MrOlm/drep") (synopsis "De-replication of microbial genomes assembled from multiple samples") (description "dRep is a Python program for rapidly comparing large numbers of genomes. dRep can also \"de-replicate\" a genome set by identifying groups of highly similar genomes and choosing the best representative genome for each genome set.") (license license:expat))) (define-public instrain (package (name "instrain") (version "1.5.2") (source (origin (method url-fetch) (uri (pypi-uri "inStrain" version)) (sha256 (base32 "0ykqlpf6yz4caihsaz3ys00cyvlr7wdj4s9a8rh56q5r8xf80ic0")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'patch-relative-imports (lambda _ (substitute* "docker/run_instrain.py" (("from s3_utils") "from .s3_utils") (("from job_utils") "from .job_utils"))))))) (inputs `(("python-biopython" ,python-biopython) ("python-boto3" ,python-boto3) ("python-h5py" ,python-h5py) ("python-lmfit" ,python-lmfit) ("python-matplotlib" ,python-matplotlib) ("python-networkx" ,python-networkx) ("python-numba" ,python-numba) ("python-numpy" ,python-numpy) ("python-pandas" ,python-pandas) ("python-psutil" ,python-psutil) ("python-pysam" ,python-pysam) ("python-scikit-learn" ,python-scikit-learn) ("python-seaborn" ,python-seaborn) ("python-tqdm" ,python-tqdm) ;; drep is needed for deprecated plot utilities ("python-drep" ,python-drep))) (native-inputs `(("python-pytest" ,python-pytest))) (home-page "https://github.com/MrOlm/inStrain") (synopsis "Calculation of strain-level metrics") (description "inStrain is a Python program for analysis of co-occurring genome populations from metagenomes that allows highly accurate genome comparisons, analysis of coverage, microdiversity, and linkage, and sensitive SNP detection with gene localization and synonymous non-synonymous identification.") ;; The tool itself says that the license is "MIT", but the repository ;; contains a LICENSE file with the GPLv3. ;; See https://github.com/MrOlm/inStrain/issues/51 (license license:expat))) (define-public gffcompare (let ((commit "be56ef4349ea3966c12c6397f85e49e047361c41") (revision "1")) (package (name "gffcompare") (version (git-version "0.10.15" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/gpertea/gffcompare/") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "0cp5qpxdhw4mxpya5dld8wi3jk00zyklm6rcri426wydinrnfmkg")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no check target #:phases (modify-phases %standard-phases (delete 'configure) (add-before 'build 'copy-gclib-source (lambda* (#:key inputs #:allow-other-keys) (mkdir "../gclib") (copy-recursively (assoc-ref inputs "gclib-source") "../gclib") #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (install-file "gffcompare" bin) #t)))))) (native-inputs `(("gclib-source" ; see 'README.md' of gffcompare ,(let ((commit "54917d0849c1e83cfb057b5f712e5cb6a35d948f") (revision "1") (name "gclib") (version (git-version "0.10.3" revision commit))) (origin (method git-fetch) (uri (git-reference (url "https://github.com/gpertea/gclib/") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "0b51lc0b8syrv7186fd7n8f15rwnf264qgfmm2palrwks1px24mr"))))))) (home-page "https://github.com/gpertea/gffcompare/") (synopsis "Tool for comparing or classifing transcripts of RNA-Seq") (description "@code{gffcompare} is a tool that can: @enumerate @item compare and evaluate the accuracy of RNA-Seq transcript assemblers (Cufflinks, Stringtie); @item collapse (merge) duplicate transcripts from multiple GTF/GFF3 files (e.g. resulted from assembly of different samples); @item classify transcripts from one or multiple GTF/GFF3 files as they relate to reference transcripts provided in a annotation file (also in GTF/GFF3 format). @end enumerate") (license (list license:expat ;license for gffcompare license:artistic2.0))))) ;license for gclib (define-public intervaltree (let ((commit "b90527f9e6d51cd36ecbb50429e4524d3a418ea5")) (package (name "intervaltree") (version (git-version "0.0.0" "1" commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ekg/intervaltree/") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "0rgv6q5fl4x5d74n6p5wvdna6zmbdbqpb4jqqh6vq3670gn08xad")))) (build-system gnu-build-system) (arguments '(#:tests? #f ; No tests. #:make-flags (list (string-append "PREFIX=" (assoc-ref %outputs "out")) "DESTDIR=\"\"") #:phases (modify-phases %standard-phases (delete 'configure)))) ; There is no configure phase. (home-page "https://github.com/ekg/intervaltree") (synopsis "Minimal C++ interval tree implementation") (description "An interval tree can be used to efficiently find a set of numeric intervals overlapping or containing another interval. This library provides a basic implementation of an interval tree using C++ templates, allowing the insertion of arbitrary types into the tree.") (license license:expat)))) (define-public python-intervaltree (package (name "python-intervaltree") (version "3.0.2") (source (origin (method url-fetch) (uri (pypi-uri "intervaltree" version)) (sha256 (base32 "0wz234g6irlm4hivs2qzmnywk0ss06ckagwh15nflkyb3p462kyb")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases ;; pytest seems to have a check to make sure the user is testing ;; their checked-out code and not an installed, potentially ;; out-of-date copy. This is harmless here, since we just installed ;; the package, so we disable the check to avoid skipping tests ;; entirely. (add-before 'check 'import-mismatch-error-workaround (lambda _ (setenv "PY_IGNORE_IMPORTMISMATCH" "1") #t))))) (propagated-inputs `(("python-sortedcontainers" ,python-sortedcontainers))) (native-inputs `(("python-pytest" ,python-pytest))) (home-page "https://github.com/chaimleib/intervaltree") (synopsis "Editable interval tree data structure") (description "This package provides a mutable, self-balancing interval tree implementation for Python. Queries may be by point, by range overlap, or by range envelopment. This library was designed to allow tagging text and time intervals, where the intervals include the lower bound but not the upper bound.") (license license:asl2.0))) (define-public python-pypairix (package (name "python-pypairix") (version "0.3.7") ;; The tarball on pypi does not include the makefile to build the ;; programs. (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/4dn-dcic/pairix") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1snr3lrmsld8sy77ng6ba6wcmd33xjccf1l2f3m6pi29xis9nd6p")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-before 'build 'build-programs (lambda _ (invoke "make"))) (add-after 'install 'install-programs (lambda* (#:key outputs #:allow-other-keys) (copy-recursively "bin" (string-append (assoc-ref outputs "out") "/bin")) #t))))) (inputs `(("zlib" ,zlib))) (home-page "https://github.com/4dn-dcic/pairix") (synopsis "Support for querying pairix-indexed bgzipped text files") (description "Pypairix is a Python module for fast querying on a pairix-indexed bgzipped text file that contains a pair of genomic coordinates per line.") (license license:expat))) (define-public python-pyfaidx (package (name "python-pyfaidx") (version "0.5.8") (source (origin (method url-fetch) (uri (pypi-uri "pyfaidx" version)) (sha256 (base32 "038xi3a6zvrxbyyfpp64ka8pcjgsdq4fgw9cl5lpxbvmm1bzzw2q")))) (build-system python-build-system) (propagated-inputs `(("python-six" ,python-six))) (home-page "http://mattshirley.com") (synopsis "Random access to fasta subsequences") (description "This package provides procedures for efficient pythonic random access to fasta subsequences.") (license license:bsd-3))) (define-public python2-pyfaidx (package-with-python2 python-pyfaidx)) (define-public python-cooler (package (name "python-cooler") (version "0.8.11") (source (origin (method url-fetch) (uri (pypi-uri "cooler" version)) (sha256 (base32 "1i96fmpsimj4wrx51rxn8lw2gqxf5a2pvrj5rwdd6ivnm3pmhyrn")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'patch-tests (lambda _ (substitute* "tests/test_create.py" (("def test_roundtrip") (string-append "@pytest.mark.skip(reason=\"requires network " "access to genome.ucsc.edu\")\n" "def test_roundtrip"))) (substitute* "tests/test_util.py" (("def test_fetch_chromsizes") (string-append "@pytest.mark.skip(reason=\"requires network " "access to genome.ucsc.edu\")\n" "def test_fetch_chromsizes"))) ;; This test depends on ipytree, which contains a lot of minified ;; JavaScript. (substitute* "tests/test_fileops.py" (("def test_print_trees") "def _test_print_trees")))) (replace 'check (lambda* (#:key tests? #:allow-other-keys) (when tests? (invoke "python" "-m" "pytest" "-v"))))))) (propagated-inputs `(("python-asciitree" ,python-asciitree) ("python-biopython" ,python-biopython) ("python-click" ,python-click) ("python-cytoolz" ,python-cytoolz) ("python-dask" ,python-dask) ("python-h5py" ,python-h5py) ("python-multiprocess" ,python-multiprocess) ("python-numpy" ,python-numpy) ("python-pandas" ,python-pandas) ("python-pyfaidx" ,python-pyfaidx) ("python-pypairix" ,python-pypairix) ("python-pysam" ,python-pysam) ("python-pyyaml" ,python-pyyaml) ("python-scipy" ,python-scipy) ("python-simplejson" ,python-simplejson) ("python-six" ,python-six) ("python-sparse" ,python-sparse))) (native-inputs `(("python-codecov" ,python-codecov) ("python-mock" ,python-mock) ("python-pytest" ,python-pytest) ("python-pytest-cov" ,python-pytest-cov) ("python-pytest-flake8" ,python-pytest-flake8))) ;; Almost all the projects of the Mirnylab are moved under Open2C umbrella (home-page "https://github.com/open2c/cooler") (synopsis "Sparse binary format for genomic interaction matrices") (description "Cooler is a support library for a sparse, compressed, binary persistent storage format, called @code{cool}, used to store genomic interaction data, such as Hi-C contact matrices.") (license license:bsd-3))) (define-public python-hicmatrix (package (name "python-hicmatrix") (version "15") (source (origin ;;Pypi sources do not contain any test (method git-fetch) (uri (git-reference (url "https://github.com/deeptools/HiCMatrix") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1dshjxgb16sdfg9k1bhw2yhyngac04k4ca7aqy8g3i3pprr068r5")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (replace 'check (lambda* (#:key tests? #:allow-other-keys) (when tests? (invoke "python" "-m" "pytest" "-v"))))))) (propagated-inputs `(("python-cooler" ,python-cooler) ("python-intervaltree" ,python-intervaltree) ("python-numpy" ,python-numpy) ("python-pandas" ,python-pandas) ("python-scipy" ,python-scipy) ("python-tables" ,python-tables))) (native-inputs `(("python-pytest" ,python-pytest))) (home-page "https://github.com/deeptools/HiCMatrix/") (synopsis "HiCMatrix class for HiCExplorer and pyGenomeTracks") (description "This helper package implements the @code{HiCMatrix} class for the HiCExplorer and pyGenomeTracks packages.") (license license:gpl3+))) (define-public python-hicexplorer (package (name "python-hicexplorer") (version "2.1.4") (source (origin ;; The latest version is not available on Pypi. (method git-fetch) (uri (git-reference (url "https://github.com/deeptools/HiCExplorer") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "0q5gpbzmrkvygqgw524q36b4nrivcmyi5v194vsx0qw7b3gcmq08")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'loosen-up-requirements (lambda _ (substitute* "setup.py" (("==") ">=")) #t))))) (propagated-inputs `(("python-biopython" ,python-biopython) ("python-configparser" ,python-configparser) ("python-cooler" ,python-cooler) ("python-future" ,python-future) ("python-intervaltree" ,python-intervaltree) ("python-jinja2" ,python-jinja2) ("python-matplotlib" ,python-matplotlib) ("python-numpy" ,python-numpy) ("python-pandas" ,python-pandas) ("python-pybigwig" ,python-pybigwig) ("python-pysam" ,python-pysam) ("python-scipy" ,python-scipy) ("python-six" ,python-six) ("python-tables" ,python-tables) ("python-unidecode" ,python-unidecode))) (home-page "https://hicexplorer.readthedocs.io") (synopsis "Process, analyze and visualize Hi-C data") (description "HiCExplorer is a powerful and easy to use set of tools to process, normalize and visualize Hi-C data. HiCExplorer facilitates the creation of contact matrices, correction of contacts, TAD detection, A/B compartments, merging, reordering or chromosomes, conversion from different formats including cooler and detection of long-range contacts. Moreover, it allows the visualization of multiple contact matrices along with other types of data like genes, compartments, ChIP-seq coverage tracks (and in general any type of genomic scores), long range contacts and the visualization of viewpoints.") (license license:gpl3))) (define-public python-pygenometracks (package (name "python-pygenometracks") (version "3.3") (source (origin (method url-fetch) (uri (pypi-uri "pyGenomeTracks" version)) (sha256 (base32 "16laa0wnf4qn9fb9ych4w1vqhqwjss70v0y0f6wp4gwqfrlgac0f")))) (build-system python-build-system) (arguments `(#:tests? #f ; there are none #:phases (modify-phases %standard-phases (add-after 'unpack 'relax-requirements (lambda _ (substitute* "setup.py" (("matplotlib ==3.1.1") "matplotlib >=3.1.1")) #t))))) (propagated-inputs `(("python-future" ,python-future) ("python-gffutils" ,python-gffutils) ("python-hicmatrix" ,python-hicmatrix) ("python-intervaltree" ,python-intervaltree) ("python-matplotlib" ,python-matplotlib) ("python-numpy" ,python-numpy) ("python-pybigwig" ,python-pybigwig) ("python-pysam" ,python-pysam) ("python-tqdm" ,python-tqdm))) (native-inputs `(("python-pytest" ,python-pytest))) (home-page "https://pygenometracks.readthedocs.io") (synopsis "Program and library to plot beautiful genome browser tracks") (description "This package aims to produce high-quality genome browser tracks that are highly customizable. Currently, it is possible to plot: bigwig, bed (many options), bedgraph, links (represented as arcs), and Hi-C matrices. pyGenomeTracks can make plots with or without Hi-C data.") (license license:gpl3+))) (define-public python-hic2cool (package (name "python-hic2cool") (version "0.8.3") ;; pypi sources do not contain the test_data directory and no test can be ;; run (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/4dn-dcic/hic2cool") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "0dlnf0qfcp4jrc1nyya32a035c13xicyq16bwfnwhbb9s47mz7gl")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases ;; Two of the test-data files need to be writable. (add-after 'unpack 'make-test-data-writable (lambda _ (for-each make-file-writable (list "test_data/hic2cool_0.4.2_single_res.cool" "test_data/hic2cool_0.7.0_multi_res.mcool"))))))) (propagated-inputs `(("python-cooler" ,python-cooler) ("python-h5py" ,python-h5py) ("python-numpy" ,python-numpy) ("python-pandas" ,python-pandas) ("python-scipy" ,python-scipy))) (home-page "https://github.com/4dn-dcic/hic2cool") (synopsis "Converter for .hic and .cool files") (description "This package provides a converter between @code{.hic} files (from juicer) and single-resolution or multi-resolution @code{.cool} files (for cooler). Both @code{hic} and @code{cool} files describe Hi-C contact matrices.") (license license:expat))) (define-public r-pore (package (name "r-pore") (version "0.24") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/rpore/" version "/poRe_" version ".tar.gz")) (sha256 (base32 "0pih9nljbv8g4x8rkk29i7aqq681b782r5s5ynp4nw9yzqnmmksv")))) (properties `((upstream-name . "poRe"))) (build-system r-build-system) (propagated-inputs `(("r-bit64" ,r-bit64) ("r-data-table" ,r-data-table) ("r-rhdf5" ,r-rhdf5) ("r-shiny" ,r-shiny) ("r-svdialogs" ,r-svdialogs))) (home-page "https://sourceforge.net/projects/rpore/") (synopsis "Visualize Nanopore sequencing data") (description "This package provides graphical user interfaces to organize and visualize Nanopore sequencing data.") ;; This is free software but the license variant is unclear: ;; . (license license:bsd-3))) (define-public r-xbioc (let ((revision "1") (commit "6ff0670a37ab3036aaf1d94aa4b208310946b0b5")) (package (name "r-xbioc") (version (git-version "0.1.16" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/renozao/xbioc") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "0w8bsq5myiwkfhh83nm6is5ichiyvwa1axx2szvxnzq39x6knf66")))) (build-system r-build-system) (propagated-inputs `(("r-annotationdbi" ,r-annotationdbi) ("r-assertthat" ,r-assertthat) ("r-biobase" ,r-biobase) ("r-biocmanager" ,r-biocmanager) ("r-digest" ,r-digest) ("r-pkgmaker" ,r-pkgmaker) ("r-plyr" ,r-plyr) ("r-reshape2" ,r-reshape2) ("r-stringr" ,r-stringr))) (home-page "https://github.com/renozao/xbioc/") (synopsis "Extra base functions for Bioconductor") (description "This package provides extra utility functions to perform common tasks in the analysis of omics data, leveraging and enhancing features provided by Bioconductor packages.") (license license:gpl3+)))) (define-public r-cssam (let ((revision "1") (commit "9ec58c982fa551af0d80b1a266890d92954833f2")) (package (name "r-cssam") (version (git-version "1.4" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/shenorrLab/csSAM") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "128syf9v39gk0z3ip000qpsjbg6l1siyq6c8b0hz41dzg5achyb3")))) (build-system r-build-system) (propagated-inputs `(("r-formula" ,r-formula) ("r-ggplot2" ,r-ggplot2) ("r-pkgmaker" ,r-pkgmaker) ("r-plyr" ,r-plyr) ("r-rngtools" ,r-rngtools) ("r-scales" ,r-scales))) (home-page "https://github.com/shenorrLab/csSAM/") (synopsis "Cell type-specific statistical analysis of microarray") (description "This package implements the method csSAM that computes cell-specific differential expression from measured cell proportions using SAM.") ;; Any version (license license:lgpl2.1+)))) (define-public r-bseqsc (let ((revision "1") (commit "fef3f3e38dcf3df37103348b5780937982b43b98")) (package (name "r-bseqsc") (version (git-version "1.0" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/shenorrLab/bseqsc") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "1prw13wa20f7wlc3gkkls66n1kxz8d28qrb8icfqdwdnnv8w5qg8")))) (build-system r-build-system) (propagated-inputs `(("r-abind" ,r-abind) ("r-annotationdbi" ,r-annotationdbi) ("r-biobase" ,r-biobase) ("r-cssam" ,r-cssam) ("r-dplyr" ,r-dplyr) ("r-e1071" ,r-e1071) ("r-edger" ,r-edger) ("r-ggplot2" ,r-ggplot2) ("r-nmf" ,r-nmf) ("r-openxlsx" ,r-openxlsx) ("r-pkgmaker" ,r-pkgmaker) ("r-plyr" ,r-plyr) ("r-preprocesscore" ,r-preprocesscore) ("r-rngtools" ,r-rngtools) ("r-scales" ,r-scales) ("r-stringr" ,r-stringr) ("r-xbioc" ,r-xbioc))) (home-page "https://github.com/shenorrLab/bseqsc") (synopsis "Deconvolution of bulk sequencing experiments using single cell data") (description "BSeq-sc is a bioinformatics analysis pipeline that leverages single-cell sequencing data to estimate cell type proportion and cell type-specific gene expression differences from RNA-seq data from bulk tissue samples. This is a companion package to the publication \"A single-cell transcriptomic map of the human and mouse pancreas reveals inter- and intra-cell population structure.\" Baron et al. Cell Systems (2016) @url{https://www.ncbi.nlm.nih.gov/pubmed/27667365}.") (license license:gpl2+)))) (define-public porechop ;; The recommended way to install is to clone the git repository ;; https://github.com/rrwick/Porechop#installation (let ((commit "289d5dca4a5fc327f97b3f8cecb68ecaf1014861") (revision "1")) (package (name "porechop") (version (git-version "0.2.3" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/rrwick/Porechop") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "05ps43gig0d3ia9x5lj84lb00hbsl6ba9n7y7jz927npxbr2ym23")))) (build-system python-build-system) (home-page "https://github.com/rrwick/porechop") (synopsis "Finding, trimming or splitting adapters, in Oxford Nanopore reads") (description "The porechop package is a tool for finding and removing adapters from Oxford Nanopore reads. Adapters on the ends of reads are trimmed off, and when a read has an adapter in its middle, it is treated as chimeric and chopped into separate reads. Porechop performs thorough alignments to effectively find adapters, even at low sequence identity. Porechop also supports demultiplexing of Nanopore reads that were barcoded with the Native Barcoding Kit, PCR Barcoding Kit or Rapid Barcoding Kit.") (license license:gpl3+)))) (define-public poretools ;; The latest release was in 2016 and the latest commit is from 2017 ;; the recommended way to install is to clone the git repository ;; https://poretools.readthedocs.io/en/latest/content/installation.html (let ((commit "e426b1f09e86ac259a00c261c79df91510777407") (revision "1")) (package (name "poretools") (version (git-version "0.6.0" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/arq5x/poretools") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "0bglj833wxpp3cq430p1d3xp085ls221js2y90w7ir2x5ay8l7am")))) (build-system python-build-system) ;; requires python >=2.7, <3.0, and the same for python dependencies (arguments `(#:python ,python-2)) (inputs `(("hdf5" ,hdf5))) (propagated-inputs `(("python-dateutil" ,python2-dateutil) ("python-h5py" ,python2-h5py) ("python-matplotlib" ,python2-matplotlib) ("python-pandas" ,python2-pandas) ("python-seaborn" ,python2-seaborn))) (home-page "https://poretools.readthedocs.io") (synopsis "Toolkit for working with nanopore sequencing data") (description "The MinION from Oxford Nanopore Technologies is a nanopore sequencer. This @code{poretools} package is a flexible toolkit for exploring datasets generated by nanopore sequencing devices for the purposes of quality control and downstream analysis. Poretools operates directly on the native FAST5, a variant of the Hierarchical Data Format (HDF5) standard.") (license license:expat)))) (define-public jamm (package (name "jamm") (version "1.0.7.6") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/mahmoudibrahim/JAMM") (commit (string-append "JAMMv" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0bsa5mf9n9q5jz7mmacrra41l7r8rac5vgsn6wv1fb52ya58b970")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are none #:phases (modify-phases %standard-phases (delete 'configure) (delete 'build) (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (libexec (string-append out "/libexec/jamm")) (bin (string-append out "/bin"))) (substitute* '("JAMM.sh" "SignalGenerator.sh") (("^sPath=.*") (string-append "sPath=\"" libexec "\"\n"))) (for-each (lambda (file) (install-file file libexec)) (list "bincalculator.r" "peakfinder.r" "peakhelper.r" "signalmaker.r" "xcorr.r" "xcorrhelper.r" ;; Perl scripts "peakfilter.pl" "readshifter.pl")) (for-each (lambda (script) (chmod script #o555) (install-file script bin) (wrap-program (string-append bin "/" script) `("PATH" ":" prefix (,(string-append (assoc-ref inputs "coreutils") "/bin") ,(string-append (assoc-ref inputs "gawk") "/bin") ,(string-append (assoc-ref inputs "perl") "/bin") ,(string-append (assoc-ref inputs "r-minimal") "/bin"))) `("PERL5LIB" ":" prefix (,(getenv "PERL5LIB"))) `("R_LIBS_SITE" ":" prefix (,(getenv "R_LIBS_SITE"))))) (list "JAMM.sh" "SignalGenerator.sh"))) #t))))) (inputs `(("bash" ,bash) ("coreutils" ,coreutils) ("gawk" ,gawk) ("perl" ,perl) ("r-minimal" ,r-minimal) ;;("r-parallel" ,r-parallel) ("r-signal" ,r-signal) ("r-mclust" ,r-mclust))) (home-page "https://github.com/mahmoudibrahim/JAMM") (synopsis "Peak finder for NGS datasets") (description "JAMM is a peak finder for next generation sequencing datasets (ChIP-Seq, ATAC-Seq, DNase-Seq, etc.) that can integrate replicates and assign peak boundaries accurately. JAMM is applicable to both broad and narrow datasets.") (license license:gpl3+))) (define-public ngless (package (name "ngless") (version "1.1.0") (source (origin (method git-fetch) (uri (git-reference (url "https://gitlab.com/ngless/ngless.git") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1wim8wpqyff080dfcazynrmjwqas38m24m0v350w245mmhrapdma")))) (build-system haskell-build-system) (arguments `(#:haddock? #f ; The haddock phase fails with: NGLess/CmdArgs.hs:20:1: ; error: parse error on input import ; import Options.Applicative #:phases (modify-phases %standard-phases (add-after 'unpack 'create-Versions.hs (lambda _ (substitute* "Makefile" (("BWA_VERSION = .*") (string-append "BWA_VERSION = " ,(package-version bwa) "\n")) (("SAM_VERSION = .*") (string-append "SAM_VERSION = " ,(package-version samtools) "\n")) (("PRODIGAL_VERSION = .*") (string-append "PRODIGAL_VERSION = " ,(package-version prodigal) "\n")) (("MINIMAP2_VERSION = .*") (string-append "MINIMAP2_VERSION = " ,(package-version minimap2) "\n"))) (invoke "make" "NGLess/Dependencies/Versions.hs") #t)) (add-after 'create-Versions.hs 'create-cabal-file (lambda _ (invoke "hpack") #t)) ;; These tools are expected to be installed alongside ngless. (add-after 'install 'link-tools (lambda* (#:key inputs outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (symlink (string-append (assoc-ref inputs "prodigal") "/bin/prodigal") (string-append bin "ngless-" ,version "-prodigal")) (symlink (string-append (assoc-ref inputs "minimap2") "/bin/minimap2") (string-append bin "ngless-" ,version "-minimap2")) (symlink (string-append (assoc-ref inputs "samtools") "/bin/samtools") (string-append bin "ngless-" ,version "-samtools")) (symlink (string-append (assoc-ref inputs "bwa") "/bin/bwa") (string-append bin "ngless-" ,version "-bwa")) #t)))))) (inputs `(("prodigal" ,prodigal) ("bwa" ,bwa) ("samtools" ,samtools) ("minimap2" ,minimap2) ("ghc-aeson" ,ghc-aeson) ("ghc-ansi-terminal" ,ghc-ansi-terminal) ("ghc-async" ,ghc-async) ("ghc-atomic-write" ,ghc-atomic-write) ("ghc-bytestring-lexing" ,ghc-bytestring-lexing) ("ghc-conduit" ,ghc-conduit) ("ghc-conduit-algorithms" ,ghc-conduit-algorithms) ("ghc-conduit-extra" ,ghc-conduit-extra) ("ghc-configurator" ,ghc-configurator) ("ghc-convertible" ,ghc-convertible) ("ghc-data-default" ,ghc-data-default) ("ghc-diagrams-core" ,ghc-diagrams-core) ("ghc-diagrams-lib" ,ghc-diagrams-lib) ("ghc-diagrams-svg" ,ghc-diagrams-svg) ("ghc-double-conversion" ,ghc-double-conversion) ("ghc-edit-distance" ,ghc-edit-distance) ("ghc-either" ,ghc-either) ("ghc-errors" ,ghc-errors) ("ghc-extra" ,ghc-extra) ("ghc-filemanip" ,ghc-filemanip) ("ghc-file-embed" ,ghc-file-embed) ("ghc-gitrev" ,ghc-gitrev) ("ghc-hashtables" ,ghc-hashtables) ("ghc-http-conduit" ,ghc-http-conduit) ("ghc-inline-c" ,ghc-inline-c) ("ghc-inline-c-cpp" ,ghc-inline-c-cpp) ("ghc-intervalmap" ,ghc-intervalmap) ("ghc-missingh" ,ghc-missingh) ("ghc-optparse-applicative" ,ghc-optparse-applicative) ("ghc-regex" ,ghc-regex) ("ghc-safe" ,ghc-safe) ("ghc-safeio" ,ghc-safeio) ("ghc-strict" ,ghc-strict) ("ghc-tar" ,ghc-tar) ("ghc-tar-conduit" ,ghc-tar-conduit) ("ghc-unliftio" ,ghc-unliftio) ("ghc-unliftio-core" ,ghc-unliftio-core) ("ghc-vector" ,ghc-vector) ("ghc-yaml" ,ghc-yaml) ("ghc-zlib" ,ghc-zlib))) (propagated-inputs `(("r-r6" ,r-r6) ("r-hdf5r" ,r-hdf5r) ("r-iterators" ,r-iterators) ("r-itertools" ,r-itertools) ("r-matrix" ,r-matrix))) (native-inputs `(("ghc-hpack" ,ghc-hpack) ("ghc-quickcheck" ,ghc-quickcheck) ("ghc-test-framework" ,ghc-test-framework) ("ghc-test-framework-hunit",ghc-test-framework-hunit) ("ghc-test-framework-quickcheck2" ,ghc-test-framework-quickcheck2) ("ghc-test-framework-th" ,ghc-test-framework-th))) (home-page "https://gitlab.com/ngless/ngless") (synopsis "DSL for processing next-generation sequencing data") (description "Ngless is a domain-specific language for @dfn{next-generation sequencing} (NGS) data processing.") (license license:expat))) (define-public filtlong ;; The recommended way to install is to clone the git repository ;; https://github.com/rrwick/Filtlong#installation ;; and the lastest release is more than nine months old (let ((commit "d1bb46dfe8bc7efe6257b5ce222c04bfe8aedaab") (revision "1")) (package (name "filtlong") (version (git-version "0.2.0" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/rrwick/Filtlong") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "1xr92r820x8qlkcr3b57iw223yq8vjgyi42jr79w2xgw47qzr575")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no check target #:phases (modify-phases %standard-phases (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (scripts (string-append out "/share/filtlong/scripts"))) (install-file "bin/filtlong" bin) (install-file "scripts/histogram.py" scripts) (install-file "scripts/read_info_histograms.sh" scripts)) #t)) (add-after 'install 'wrap-program (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (path (getenv "PYTHONPATH"))) (wrap-program (string-append out "/share/filtlong/scripts/histogram.py") `("PYTHONPATH" ":" prefix (,path)))) #t)) (add-before 'check 'patch-tests (lambda _ (substitute* "scripts/read_info_histograms.sh" (("awk") (which "gawk"))) #t))))) (inputs `(("gawk" ,gawk) ;for read_info_histograms.sh ("python" ,python-2) ;required for histogram.py ("zlib" ,zlib))) (home-page "https://github.com/rrwick/Filtlong/") (synopsis "Tool for quality filtering of Nanopore and PacBio data") (description "The Filtlong package is a tool for filtering long reads by quality. It can take a set of long reads and produce a smaller, better subset. It uses both read length (longer is better) and read identity (higher is better) when choosing which reads pass the filter.") (license (list license:gpl3 ;filtlong license:asl2.0))))) ;histogram.py (define-public nanopolish ;; The recommended way to install is to clone the git repository ;; . ;; Also, the differences between release and current version seem to be ;; significant. (let ((commit "6331dc4f15b9dfabb954ba3fae9d76b6c3ca6377") (revision "1")) (package (name "nanopolish") (version (git-version "0.11.1" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/jts/nanopolish") (commit commit) (recursive? #t))) (file-name (git-file-name name version)) (sha256 (base32 "15ikl3d37y49pwd7vx36xksgsqajhf24q7qqsnpl15dqqyy5qgbc")) (modules '((guix build utils))) (snippet '(begin (delete-file-recursively "htslib") #t)))) (build-system gnu-build-system) (arguments `(#:make-flags `("HDF5=noinstall" "EIGEN=noinstall" "HTS=noinstall" "CC=gcc") #:tests? #f ; no check target #:phases (modify-phases %standard-phases (add-after 'unpack 'find-eigen (lambda* (#:key inputs #:allow-other-keys) (setenv "CPATH" (string-append (assoc-ref inputs "eigen") "/include/eigen3:" (or (getenv "CPATH") ""))) #t)) (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (scripts (string-append out "/share/nanopolish/scripts"))) (install-file "nanopolish" bin) (for-each (lambda (file) (install-file file scripts)) (find-files "scripts" ".*")) #t))) (add-after 'install 'wrap-programs (lambda* (#:key outputs #:allow-other-keys) (let ((pythonpath (getenv "PYTHONPATH")) (perl5lib (getenv "PERL5LIB")) (scripts (string-append (assoc-ref outputs "out") "/share/nanopolish/scripts"))) (for-each (lambda (file) (wrap-program file `("PYTHONPATH" ":" prefix (,pythonpath)))) (find-files scripts "\\.py")) (for-each (lambda (file) (wrap-script file `("PERL5LIB" ":" prefix (,perl5lib)))) (find-files scripts "\\.pl")))))))) (inputs `(("guile" ,guile-3.0) ; for wrappers ("eigen" ,eigen) ("hdf5" ,hdf5) ("htslib" ,htslib) ("perl" ,perl) ("bioperl" ,bioperl-minimal) ("perl-getopt-long" ,perl-getopt-long) ("python" ,python-wrapper) ("python-biopython" ,python-biopython) ("python-numpy" ,python-numpy) ("python-pysam" ,python-pysam) ("python-scikit-learn" , python-scikit-learn) ("python-scipy" ,python-scipy) ("zlib" ,zlib))) (home-page "https://github.com/jts/nanopolish") (synopsis "Signal-level analysis of Oxford Nanopore sequencing data") (description "This package analyses the Oxford Nanopore sequencing data at signal-level. Nanopolish can calculate an improved consensus sequence for a draft genome assembly, detect base modifications, call SNPs (Single nucleotide polymorphisms) and indels with respect to a reference genome and more.") (license license:expat)))) (define-public cnvkit (package (name "cnvkit") (version "0.9.5") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/etal/cnvkit") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0g2f78k68yglmj4fsfmgs8idqv3di9aj53fg0ld0hqljg8chhh82")))) (build-system python-build-system) (propagated-inputs `(("python-biopython" ,python-biopython) ("python-future" ,python-future) ("python-matplotlib" ,python-matplotlib) ("python-numpy" ,python-numpy) ("python-reportlab" ,python-reportlab) ("python-pandas" ,python-pandas) ("python-pysam" ,python-pysam) ("python-pyfaidx" ,python-pyfaidx) ("python-scipy" ,python-scipy) ;; R packages ("r-dnacopy" ,r-dnacopy))) (home-page "https://cnvkit.readthedocs.org/") (synopsis "Copy number variant detection from targeted DNA sequencing") (description "CNVkit is a Python library and command-line software toolkit to infer and visualize copy number from high-throughput DNA sequencing data. It is designed for use with hybrid capture, including both whole-exome and custom target panels, and short-read sequencing platforms such as Illumina and Ion Torrent.") (license license:asl2.0))) (define-public python-pyfit-sne (package (name "python-pyfit-sne") (version "1.0.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/KlugerLab/pyFIt-SNE") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "13wh3qkzs56azmmgnxib6xfr29g7xh09sxylzjpni5j0pp0rc5qw")))) (build-system python-build-system) (propagated-inputs `(("python-numpy" ,python-numpy))) (inputs `(("fftw" ,fftw))) (native-inputs `(("python-cython" ,python-cython))) (home-page "https://github.com/KlugerLab/pyFIt-SNE") (synopsis "FFT-accelerated Interpolation-based t-SNE") (description "t-Stochastic Neighborhood Embedding (t-SNE) is a highly successful method for dimensionality reduction and visualization of high dimensional datasets. A popular implementation of t-SNE uses the Barnes-Hut algorithm to approximate the gradient at each iteration of gradient descent. This package is a Cython wrapper for FIt-SNE.") (license license:bsd-4))) (define-public bbmap (package (name "bbmap") (version "38.90") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/bbmap/BBMap_" version ".tar.gz")) (sha256 (base32 "1wb94bcc006qq86x77z2rz0lc8m9f1kpnw6gdhjfg9bdaqf56rm3")))) (build-system ant-build-system) (arguments `(#:build-target "dist" #:tests? #f ; there are none #:make-flags (list (string-append "-Dmpijar=" (assoc-ref %build-inputs "java-openmpi") "/lib/mpi.jar")) #:modules ((guix build ant-build-system) (guix build utils) (guix build java-utils)) #:phases (modify-phases %standard-phases (add-after 'build 'build-jni-library (lambda _ (with-directory-excursion "jni" (invoke "make" "-f" "makefile.linux")))) ;; There is no install target (replace 'install (install-jars "dist")) (add-after 'install 'install-scripts-and-documentation (lambda* (#:key outputs #:allow-other-keys) (substitute* "calcmem.sh" (("\\| awk ") (string-append "| " (which "awk") " "))) (let* ((scripts (find-files "." "\\.sh$")) (out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (doc (string-append out "/share/doc/bbmap")) (jni (string-append out "/lib/jni"))) (substitute* scripts (("\\$DIR\"\"docs") doc) (("^CP=.*") (string-append "CP=" out "/share/java/BBTools.jar\n")) (("^NATIVELIBDIR.*") (string-append "NATIVELIBDIR=" jni "\n")) (("CMD=\"java") (string-append "CMD=\"" (which "java")))) (for-each (lambda (script) (install-file script bin)) scripts) ;; Install JNI library (install-file "jni/libbbtoolsjni.so" jni) ;; Install documentation (install-file "docs/readme.txt" doc) (copy-recursively "docs/guides" doc)) #t))) #:jdk ,openjdk11)) (inputs `(("gawk" ,gawk) ("java-eclipse-jdt-core" ,java-eclipse-jdt-core) ("java-eclipse-jdt-compiler-apt" ,java-eclipse-jdt-compiler-apt) ("java-openmpi" ,java-openmpi))) (home-page "https://sourceforge.net/projects/bbmap/") (synopsis "Aligner and other tools for short sequencing reads") (description "This package provides bioinformatic tools to align, deduplicate, reformat, filter and normalize DNA and RNA-seq data. It includes the following tools: BBMap, a short read aligner for DNA and RNA-seq data; BBNorm, a kmer-based error-correction and normalization tool; Dedupe, a tool to simplify assemblies by removing duplicate or contained subsequences that share a target percent identity; Reformat, to convert reads between fasta/fastq/scarf/fasta+qual/sam, interleaved/paired, and ASCII-33/64, at over 500 MB/s; and BBDuk, a tool to filter, trim, or mask reads with kmer matches to an artifact/contaminant file.") (license license:bsd-3))) (define-public velvet (package (name "velvet") (version "1.2.10") (source (origin (method url-fetch) (uri (string-append "https://www.ebi.ac.uk/~zerbino/velvet/" "velvet_" version ".tgz")) (sha256 (base32 "0h3njwy66p6bx14r3ar1byb0ccaxmxka4c65rn4iybyiqa4d8kc8")) ;; Delete bundled libraries (modules '((guix build utils))) (snippet '(begin (delete-file "Manual.pdf") (delete-file-recursively "third-party") #t)))) (build-system gnu-build-system) (arguments `(#:make-flags '("OPENMP=t") #:test-target "test" #:phases (modify-phases %standard-phases (delete 'configure) (add-after 'unpack 'fix-zlib-include (lambda _ (substitute* "src/binarySequences.c" (("../third-party/zlib-1.2.3/zlib.h") "zlib.h")) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (doc (string-append out "/share/doc/velvet"))) (mkdir-p bin) (mkdir-p doc) (install-file "velveth" bin) (install-file "velvetg" bin) (install-file "Manual.pdf" doc) (install-file "Columbus_manual.pdf" doc) #t)))))) (inputs `(("openmpi" ,openmpi) ("zlib" ,zlib))) (native-inputs `(("texlive" ,(texlive-union (list texlive-latex-graphics texlive-latex-hyperref))))) (home-page "https://www.ebi.ac.uk/~zerbino/velvet/") (synopsis "Nucleic acid sequence assembler for very short reads") (description "Velvet is a de novo genomic assembler specially designed for short read sequencing technologies, such as Solexa or 454. Velvet currently takes in short read sequences, removes errors then produces high quality unique contigs. It then uses paired read information, if available, to retrieve the repeated areas between contigs.") (license license:gpl2+))) (define-public python-velocyto (package (name "python-velocyto") (version "0.17.17") (source (origin (method url-fetch) (uri (pypi-uri "velocyto" version)) (sha256 (base32 "0fgygyzqgrq32dv6a00biq1p1cwi6kbl5iqblxq1kklj6b2mzmhs")))) (build-system python-build-system) (native-inputs `(("python-joblib" ,python-joblib))) (propagated-inputs `(("python-click" ,python-click) ("python-cython" ,python-cython) ("python-h5py" ,python-h5py) ("python-loompy" ,python-loompy) ("python-matplotlib" ,python-matplotlib) ("python-numba" ,python-numba) ("python-numpy" ,python-numpy) ("python-pandas" ,python-pandas) ("python-pysam" ,python-pysam) ("python-scikit-learn" ,python-scikit-learn) ("python-scipy" ,python-scipy))) (home-page "https://github.com/velocyto-team/velocyto.py") (synopsis "RNA velocity analysis for single cell RNA-seq data") (description "Velocyto is a library for the analysis of RNA velocity. Velocyto includes a command line tool and an analysis pipeline.") (license license:bsd-2))) (define-public arriba (package (name "arriba") (version "1.0.1") (source (origin (method url-fetch) (uri (string-append "https://github.com/suhrig/arriba/releases/" "download/v" version "/arriba_v" version ".tar.gz")) (sha256 (base32 "0jx9656ry766vb8z08m1c3im87b0c82qpnjby9wz4kcz8vn87dx2")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are none #:phases (modify-phases %standard-phases (replace 'configure (lambda* (#:key inputs #:allow-other-keys) (let ((htslib (assoc-ref inputs "htslib"))) (substitute* "Makefile" (("-I\\$\\(HTSLIB\\)/htslib") (string-append "-I" htslib "/include/htslib")) ((" \\$\\(HTSLIB\\)/libhts.a") (string-append " " htslib "/lib/libhts.so")))) (substitute* "run_arriba.sh" (("^STAR ") (string-append (which "STAR") " ")) (("samtools --version-only") (string-append (which "samtools") " --version-only")) (("samtools index") (string-append (which "samtools") " index")) (("samtools sort") (string-append (which "samtools") " sort"))) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (install-file "arriba" bin) (install-file "run_arriba.sh" bin) (install-file "draw_fusions.R" bin) (wrap-program (string-append bin "/draw_fusions.R") `("R_LIBS_SITE" ":" prefix (,(getenv "R_LIBS_SITE"))))) #t))))) (inputs `(("htslib" ,htslib) ("r-minimal" ,r-minimal) ("r-circlize" ,r-circlize) ("r-genomicalignments" ,r-genomicalignments) ("r-genomicranges" ,r-genomicranges) ("samtools" ,samtools) ("star" ,star) ("zlib" ,zlib))) (home-page "https://github.com/suhrig/arriba") (synopsis "Gene fusion detection from RNA-Seq data ") (description "Arriba is a command-line tool for the detection of gene fusions from RNA-Seq data. It was developed for the use in a clinical research setting. Therefore, short runtimes and high sensitivity were important design criteria. It is based on the fast STAR aligner and the post-alignment runtime is typically just around two minutes. In contrast to many other fusion detection tools which build on STAR, Arriba does not require to reduce the @code{alignIntronMax} parameter of STAR to detect small deletions.") ;; All code is under the Expat license with the exception of ;; "draw_fusions.R", which is under GPLv3. (license (list license:expat license:gpl3)))) (define-public adapterremoval (package (name "adapterremoval") (version "2.3.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/MikkelSchubert/adapterremoval") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1nf3ki5pfzalhrx2fr1y6pfqfi133yj2m7q4fj9irf5fb94bapwr")))) (build-system gnu-build-system) (arguments `(#:make-flags (list "COLOR_BUILD=no" (string-append "PREFIX=" (assoc-ref %outputs "out"))) #:test-target "test" #:phases (modify-phases %standard-phases (delete 'configure)))) (inputs `(("zlib" ,zlib))) (home-page "https://adapterremoval.readthedocs.io/") (synopsis "Rapid sequence adapter trimming, identification, and read merging") (description "This program searches for and removes remnant adapter sequences from @dfn{High-Throughput Sequencing} (HTS) data and (optionally) trims low quality bases from the 3' end of reads following adapter removal. AdapterRemoval can analyze both single end and paired end data, and can be used to merge overlapping paired-ended reads into (longer) consensus sequences. Additionally, the AdapterRemoval may be used to recover a consensus adapter sequence for paired-ended data, for which this information is not available.") (license license:gpl3+))) (define-public pplacer (let ((commit "807f6f3")) (package (name "pplacer") ;; The commit should be updated with each version change. (version "1.1.alpha19") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/matsen/pplacer") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "11ppbbbx20p2g9wj3ff64dhnarb12q79v7qh4rk0gj6lkbz4n7cn")))) (build-system ocaml-build-system) (arguments `(#:modules ((guix build ocaml-build-system) (guix build utils) (ice-9 ftw)) #:phases (modify-phases %standard-phases (delete 'configure) (add-after 'unpack 'fix-build-with-latest-ocaml (lambda _ (substitute* "myocamlbuild.ml" (("dep \\[\"c_pam\"\\]" m) (string-append "flag [\"ocaml\"; \"compile\"] (A \"-unsafe-string\");\n" m)) (("let run_and_read" m) (string-append " let split s ch = let x = ref [] in let rec go s = let pos = String.index s ch in x := (String.before s pos)::!x; go (String.after s (pos + 1)) in try go s with Not_found -> !x let split_nl s = split s '\\n' let before_space s = try String.before s (String.index s ' ') with Not_found -> s " m)) (("run_and_read \"ocamlfind list \\| cut -d' ' -f1\"" m) (string-append "List.map before_space (split_nl & " m ")")) ((" blank_sep_strings &") "") ((" Lexing.from_string &") "")) #t)) (add-after 'unpack 'replace-bundled-cddlib (lambda* (#:key inputs #:allow-other-keys) (let* ((cddlib-src (assoc-ref inputs "cddlib-src")) (local-dir "cddlib_guix")) (mkdir local-dir) (with-directory-excursion local-dir (invoke "tar" "xvf" cddlib-src)) (let ((cddlib-src-folder (string-append local-dir "/" (list-ref (scandir local-dir) 2) "/lib-src"))) (for-each make-file-writable (find-files "cdd_src" ".*")) (for-each (lambda (file) (copy-file file (string-append "cdd_src/" (basename file)))) (find-files cddlib-src-folder ".*[ch]$"))) #t))) (add-after 'unpack 'fix-makefile (lambda _ ;; Remove system calls to 'git'. (substitute* "Makefile" (("^DESCRIPT:=pplacer-.*") (string-append "DESCRIPT:=pplacer-$(shell uname)-v" ,version "\n"))) (substitute* "myocamlbuild.ml" (("git describe --tags --long .*\\\" with") (string-append "echo -n v" ,version "-" ,commit "\" with"))) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin"))) (copy-recursively "bin" bin)) #t))) #:ocaml ,ocaml-4.07 #:findlib ,ocaml4.07-findlib)) (inputs `(("zlib" ,zlib "static") ("gsl" ,gsl) ("ocaml-ounit" ,(package-with-ocaml4.07 ocaml-ounit)) ("ocaml-batteries" ,(package-with-ocaml4.07 ocaml-batteries)) ("ocaml-camlzip" ,(package-with-ocaml4.07 camlzip)) ("ocaml-csv" ,(package-with-ocaml4.07 ocaml-csv)) ("ocaml-sqlite3" ,ocaml4.07-sqlite3) ("ocaml-xmlm" ,(package-with-ocaml4.07 ocaml-xmlm)) ("ocaml-mcl" ,(package-with-ocaml4.07 ocaml-mcl)) ("ocaml-gsl" ,ocaml4.07-gsl-1))) (native-inputs `(("cddlib-src" ,(package-source cddlib)) ("ocamlbuild" ,(package-with-ocaml4.07 ocamlbuild)) ("pkg-config" ,pkg-config))) (propagated-inputs `(("pplacer-scripts" ,pplacer-scripts))) (synopsis "Phylogenetic placement of biological sequences") (description "Pplacer places query sequences on a fixed reference phylogenetic tree to maximize phylogenetic likelihood or posterior probability according to a reference alignment. Pplacer is designed to be fast, to give useful information about uncertainty, and to offer advanced visualization and downstream analysis.") (home-page "https://matsen.fhcrc.org/pplacer/") (license license:gpl3)))) ;; This package is installed alongside 'pplacer'. It is a separate package so ;; that it can use the python-build-system for the scripts that are ;; distributed alongside the main OCaml binaries. (define pplacer-scripts (package (inherit pplacer) (name "pplacer-scripts") (build-system python-build-system) (arguments `(#:python ,python-2 #:phases (modify-phases %standard-phases (add-after 'unpack 'enter-scripts-dir (lambda _ (chdir "scripts") #t)) (replace 'check (lambda _ (invoke "python" "-m" "unittest" "discover" "-v") #t)) (add-after 'install 'wrap-executables (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin"))) (let ((path (string-append (assoc-ref inputs "hmmer") "/bin:" (assoc-ref inputs "infernal") "/bin"))) (display path) (wrap-program (string-append bin "/refpkg_align.py") `("PATH" ":" prefix (,path)))) (let ((path (string-append (assoc-ref inputs "hmmer") "/bin"))) (wrap-program (string-append bin "/hrefpkg_query.py") `("PATH" ":" prefix (,path))))) #t))))) (inputs `(("infernal" ,infernal) ("hmmer" ,hmmer))) (propagated-inputs `(("python-biopython" ,python2-biopython) ("taxtastic" ,taxtastic))) (synopsis "Pplacer Python scripts"))) (define-public python2-checkm-genome (package (name "python2-checkm-genome") (version "1.0.13") (source (origin (method url-fetch) (uri (pypi-uri "checkm-genome" version)) (sha256 (base32 "0bm8gpxjmzxsxxl8lzwqhgx8g1dlnmp6znz7wv3hgb0gdjbf9dzz")))) (build-system python-build-system) (arguments `(#:python ,python-2 #:tests? #f)) ; some tests are interactive (propagated-inputs `(("python-dendropy" ,python2-dendropy) ("python-matplotlib" ,python2-matplotlib) ("python-numpy" ,python2-numpy) ("python-pysam" ,python2-pysam) ("python-scipy" ,python2-scipy))) (home-page "https://pypi.org/project/Checkm/") (synopsis "Assess the quality of putative genome bins") (description "CheckM provides a set of tools for assessing the quality of genomes recovered from isolates, single cells, or metagenomes. It provides robust estimates of genome completeness and contamination by using collocated sets of genes that are ubiquitous and single-copy within a phylogenetic lineage. Assessment of genome quality can also be examined using plots depicting key genomic characteristics (e.g., GC, coding density) which highlight sequences outside the expected distributions of a typical genome. CheckM also provides tools for identifying genome bins that are likely candidates for merging based on marker set compatibility, similarity in genomic characteristics, and proximity within a reference genome.") (license license:gpl3+))) (define-public umi-tools (package (name "umi-tools") (version "1.0.0") (source (origin (method url-fetch) (uri (pypi-uri "umi_tools" version)) (sha256 (base32 "08y3vz1vcx09whmbsn722lcs6jl9wyrh9i4p3k8j4cb1i32bij4a")))) (build-system python-build-system) (inputs `(("python-pandas" ,python-pandas) ("python-future" ,python-future) ("python-scipy" ,python-scipy) ("python-matplotlib" ,python-matplotlib) ("python-regex" ,python-regex) ("python-pysam" ,python-pysam))) (native-inputs `(("python-cython" ,python-cython))) (home-page "https://github.com/CGATOxford/UMI-tools") (synopsis "Tools for analyzing unique modular identifiers") (description "This package provides tools for dealing with @dfn{Unique Molecular Identifiers} (UMIs) and @dfn{Random Molecular Tags} (RMTs) in genetic sequences. There are six tools: the @code{extract} and @code{whitelist} commands are used to prepare a fastq containing UMIs @code{+/-} cell barcodes for alignment. The remaining commands, @code{group}, @code{dedup}, and @{count}/@code{count_tab}, are used to identify PCR duplicates using the UMIs and perform different levels of analysis depending on the needs of the user.") (license license:expat))) (define-public ataqv (package (name "ataqv") (version "1.0.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ParkerLab/ataqv") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "031xr6jx1aprh26y5b1lv3gzrlmzg4alfl73vvshymx8cq8asrqi")))) (build-system gnu-build-system) (arguments `(#:make-flags (list (string-append "prefix=" (assoc-ref %outputs "out")) (string-append "BOOST_ROOT=" (assoc-ref %build-inputs "boost")) (string-append "HTSLIB_ROOT=" (assoc-ref %build-inputs "htslib"))) #:test-target "test" #:phases (modify-phases %standard-phases (delete 'configure)))) (inputs `(("boost" ,boost) ("htslib" ,htslib) ("ncurses" ,ncurses) ("zlib" ,zlib))) (native-inputs `(("lcov" ,lcov))) (home-page "https://github.com/ParkerLab/ataqv") (synopsis "Toolkit for quality control and visualization of ATAC-seq data") (description "This package provides a toolkit for measuring and comparing ATAC-seq results. It was written to make it easier to spot differences that might be caused by ATAC-seq library prep or sequencing. The main program, @code{ataqv}, examines aligned reads and reports some basic metrics.") (license license:gpl3+))) (define-public r-psiplot (package (name "r-psiplot") (version "2.3.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/kcha/psiplot") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "08438h16cfry5kqh3y9hs8q1b1a8bxhblsm75knviz5r6q0n1jxh")))) (build-system r-build-system) (propagated-inputs `(("r-mass" ,r-mass) ("r-dplyr" ,r-dplyr) ("r-tidyr" ,r-tidyr) ("r-purrr" ,r-purrr) ("r-readr" ,r-readr) ("r-magrittr" ,r-magrittr) ("r-ggplot2" ,r-ggplot2))) (home-page "https://github.com/kcha/psiplot") (synopsis "Plot percent spliced-in values of alternatively-spliced exons") (description "PSIplot is an R package for generating plots of @dfn{percent spliced-in} (PSI) values of alternatively-spliced exons that were computed by vast-tools, an RNA-Seq pipeline for alternative splicing analysis. The plots are generated using @code{ggplot2}.") (license license:expat))) (define-public python-ont-fast5-api (package (name "python-ont-fast5-api") (version "1.4.4") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/nanoporetech/ont_fast5_api") (commit (string-append "release_" version)))) (file-name (git-file-name name version)) (sha256 (base32 "03cbq4zbbwhll8ml2m9k8sa31mirsvcbjkrq1yna0kkzz9fad5fm")))) (build-system python-build-system) (propagated-inputs `(("python-numpy" ,python-numpy) ("python-six" ,python-six) ("python-h5py" ,python-h5py) ("python-progressbar33" ,python-progressbar33))) (home-page "https://github.com/nanoporetech/ont_fast5_api") (synopsis "Interface to HDF5 files of the Oxford Nanopore fast5 file format") (description "This package provides a concrete implementation of the fast5 file schema using the generic @code{h5py} library, plain-named methods to interact with and reflect the fast5 file schema, and tools to convert between @code{multi_read} and @code{single_read} formats.") (license license:mpl2.0))) (define-public tbsp (let ((commit "ec8fff4410cfb13a677dbbb95cbbc60217e64907") (revision "1")) (package (name "tbsp") (version (git-version "1.0.0" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/phoenixding/tbsp") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "025ym14x8gbd6hb55lsinqj6f5qzw36i10klgs7ldzxxd7s39ki1")))) (build-system python-build-system) (arguments '(#:tests? #f)) ; no tests included (inputs `(("python-matplotlib" ,python-matplotlib) ("python-networkx" ,python-networkx) ("python-numpy" ,python-numpy) ("python-pybigwig" ,python-pybigwig) ("python-biopython" ,python-biopython) ("python-scikit-learn" ,python-scikit-learn) ("python-scipy" ,python-scipy))) (home-page "https://github.com/phoenixding/tbsp/") (synopsis "SNP-based trajectory inference") (description "Several studies focus on the inference of developmental and response trajectories from single cell RNA-Seq (scRNA-Seq) data. A number of computational methods, often referred to as pseudo-time ordering, have been developed for this task. CRISPR has also been used to reconstruct lineage trees by inserting random mutations. The tbsp package implements an alternative method to detect significant, cell type specific sequence mutations from scRNA-Seq data.") (license license:expat)))) (define-public tabixpp (package (name "tabixpp") (version "1.1.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ekg/tabixpp") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1k2a3vbq96ic4lw72iwp5s3mwwc4xhdffjj584yn6l9637q9j1yd")) (modules '((guix build utils))) (snippet `(begin (delete-file-recursively "htslib") #t)))) (build-system gnu-build-system) (inputs `(("htslib" ,htslib) ("zlib" ,zlib))) (arguments `(#:tests? #f ; There are no tests to run. #:phases (modify-phases %standard-phases (delete 'configure) ; There is no configure phase. ;; The build phase needs overriding the location of htslib. (replace 'build (lambda* (#:key inputs #:allow-other-keys) (let ((htslib-ref (assoc-ref inputs "htslib"))) (invoke "make" (string-append "HTS_LIB=" htslib-ref "/lib/libhts.a") (string-append "INCLUDES= -I" htslib-ref "/include/htslib") "HTS_HEADERS=" ; No need to check for headers here. (string-append "LIBPATH=-L. -L" htslib-ref "/include")) (invoke "g++" "-shared" "-o" "libtabixpp.so" "tabix.o" "-lhts") (invoke "ar" "rcs" "libtabixpp.a" "tabix.o")))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (lib (string-append out "/lib")) (bin (string-append out "/bin"))) (install-file "tabix++" bin) (install-file "libtabixpp.so" lib) (install-file "libtabixpp.a" lib) (install-file "tabix.hpp" (string-append out "/include")) (mkdir-p (string-append lib "/pkgconfig")) (with-output-to-file (string-append lib "/pkgconfig/tabixpp.pc") (lambda _ (format #t "prefix=~a~@ exec_prefix=${prefix}~@ libdir=${exec_prefix}/lib~@ includedir=${prefix}/include~@ ~@ ~@ Name: libtabixpp~@ Version: ~a~@ Description: C++ wrapper around tabix project~@ Libs: -L${libdir} -ltabixpp~@ Cflags: -I${includedir}~%" out ,version))) #t)))))) (home-page "https://github.com/ekg/tabixpp") (synopsis "C++ wrapper around tabix project") (description "This is a C++ wrapper around the Tabix project which abstracts some of the details of opening and jumping in tabix-indexed files.") (license license:expat))) (define-public smithwaterman (let ((commit "2610e259611ae4cde8f03c72499d28f03f6d38a7")) (package (name "smithwaterman") (version (git-version "0.0.0" "2" commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ekg/smithwaterman/") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "0i9d8zrxpiracw3mxzd9siybpy62p06rqz9mc2w93arajgbk45bs")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; There are no tests to run. #:make-flags '("libsw.a" "all") #:phases (modify-phases %standard-phases (delete 'configure) ; There is no configure phase. (add-after 'unpack 'patch-source (lambda _ (substitute* "Makefile" (("-c ") "-c -fPIC ")) #t)) (add-after 'build 'build-dynamic (lambda _ (invoke "g++" "-shared" "-o" "libsmithwaterman.so" "smithwaterman.o" "SmithWatermanGotoh.o" "disorder.o" "BandedSmithWaterman.o" "LeftAlign.o" "Repeats.o" "IndelAllele.o"))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (lib (string-append out "/lib"))) (install-file "smithwaterman" bin) (for-each (lambda (file) (install-file file (string-append out "/include/smithwaterman"))) (find-files "." "\\.h$")) (install-file "libsmithwaterman.so" lib) (install-file "libsw.a" lib) (mkdir-p (string-append lib "/pkgconfig")) (with-output-to-file (string-append lib "/pkgconfig/smithwaterman.pc") (lambda _ (format #t "prefix=~a~@ exec_prefix=${prefix}~@ libdir=${exec_prefix}/lib~@ includedir=${prefix}/include/smithwaterman~@ ~@ ~@ Name: smithwaterman~@ Version: ~a~@ Description: smith-waterman-gotoh alignment algorithm~@ Libs: -L${libdir} -lsmithwaterman~@ Cflags: -I${includedir}~%" out ,version)))) #t))))) (home-page "https://github.com/ekg/smithwaterman") (synopsis "Implementation of the Smith-Waterman algorithm") (description "Implementation of the Smith-Waterman algorithm.") ;; The licensing terms are unclear: https://github.com/ekg/smithwaterman/issues/9. (license (list license:gpl2 license:expat))))) (define-public multichoose (package (name "multichoose") (version "1.0.3") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ekg/multichoose/") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0ci5fqvmpamwgxvmyd79ygj6n3bnbl3vc7b6h1sxz58186sm3pfs")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; Tests require node. #:phases (modify-phases %standard-phases (delete 'configure) ; There is no configure phase. (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (include (string-append out "/include"))) ;; TODO: There are Python modules for these programs too. (install-file "multichoose" bin) (install-file "multipermute" bin) (install-file "multichoose.h" include) (install-file "multipermute.h" include)) #t))))) (home-page "https://github.com/ekg/multichoose") (synopsis "Efficient loopless multiset combination generation algorithm") (description "This library implements an efficient loopless multiset combination generation algorithm which is (approximately) described in \"Loopless algorithms for generating permutations, combinations, and other combinatorial configurations.\", G. Ehrlich - Journal of the ACM (JACM), 1973. (Algorithm 7.)") (license license:expat))) (define-public fsom (let ((commit "a6ef318fbd347c53189384aef7f670c0e6ce89a3")) (package (name "fsom") (version (git-version "0.0.0" "1" commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ekg/fsom/") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "0gw1lpvr812pywg9y546x0h1hhj261xwls41r6kqhddjlrcjc0pi")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; There are no tests to run. #:phases (modify-phases %standard-phases (delete 'configure) ; There is no configure phase. (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (install-file "fsom" bin)) #t))))) (home-page "https://github.com/ekg/fsom") (synopsis "Manage SOM (Self-Organizing Maps) neural networks") (description "A tiny C library for managing SOM (Self-Organizing Maps) neural networks.") (license license:gpl3)))) (define-public fastahack (package (name "fastahack") (version "1.0.0") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/ekg/fastahack/") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0rp1blskhzxf7vbh253ibpxbgl9wwgyzf1wbkxndi08d3j4vcss9")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; Unclear how to run tests: https://github.com/ekg/fastahack/issues/15 #:phases (modify-phases %standard-phases (delete 'configure) ; There is no configure phase. (add-after 'unpack 'patch-source (lambda _ (substitute* "Makefile" (("-c ") "-c -fPIC ")) #t)) (add-after 'build 'build-dynamic (lambda _ (invoke "g++" "-shared" "-o" "libfastahack.so" "Fasta.o" "FastaHack.o" "split.o" "disorder.o"))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (lib (string-append out "/lib")) (bin (string-append out "/bin"))) (mkdir-p (string-append out "/include/fastahack")) (for-each (lambda (file) (install-file file (string-append out "/include/fastahack"))) (find-files "." "\\.h$")) (install-file "fastahack" bin) (install-file "libfastahack.so" lib) (mkdir-p (string-append lib "/pkgconfig")) (with-output-to-file (string-append lib "/pkgconfig/fastahack.pc") (lambda _ (format #t "prefix=~a~@ exec_prefix=${prefix}~@ libdir=${exec_prefix}/lib~@ includedir=${prefix}/include/fastahack~@ ~@ ~@ Name: fastahack~@ Version: ~a~@ Description: Indexing and sequence extraction from FASTA files~@ Libs: -L${libdir} -lfastahack~@ Cflags: -I${includedir}~%" out ,version)))) #t))))) (home-page "https://github.com/ekg/fastahack") (synopsis "Indexing and sequence extraction from FASTA files") (description "Fastahack is a small application for indexing and extracting sequences and subsequences from FASTA files. The included library provides a FASTA reader and indexer that can be embedded into applications which would benefit from directly reading subsequences from FASTA files. The library automatically handles index file generation and use.") (license (list license:expat license:gpl2)))) (define-public vcflib (package (name "vcflib") (version "1.0.2") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/vcflib/vcflib") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1k1z3876kbzifj1sqfzsf3lgb4rw779hvkg6ryxbyq5bc2paj9kh")) (modules '((guix build utils))) (snippet '(begin (substitute* "CMakeLists.txt" ((".*fastahack.*") "") ((".*smithwaterman.*") "") (("(pkg_check_modules\\(TABIXPP)" text) (string-append "pkg_check_modules(FASTAHACK REQUIRED fastahack)\n" "pkg_check_modules(SMITHWATERMAN REQUIRED smithwaterman)\n" text)) (("\\$\\{TABIXPP_LIBRARIES\\}" text) (string-append "${FASTAHACK_LIBRARIES} " "${SMITHWATERMAN_LIBRARIES} " text))) (substitute* (find-files "." "\\.(h|c)(pp)?$") (("\"SmithWatermanGotoh.h\"") "") (("\"convert.h\"") "") (("\"disorder.h\"") "") (("Fasta.h") "fastahack/Fasta.h")) (for-each delete-file-recursively '("fastahack" "filevercmp" "fsom" "googletest" "intervaltree" "libVCFH" "multichoose" "smithwaterman")) #t)))) (build-system cmake-build-system) (inputs `(("bzip2" ,bzip2) ("htslib" ,htslib) ("fastahack" ,fastahack) ("perl" ,perl) ("python" ,python) ("smithwaterman" ,smithwaterman) ("tabixpp" ,tabixpp) ("xz" ,xz) ("zlib" ,zlib))) (native-inputs `(("pkg-config" ,pkg-config) ;; Submodules. ;; This package builds against the .o files so we need to extract the source. ("filevercmp-src" ,(package-source filevercmp)) ("fsom-src" ,(package-source fsom)) ("intervaltree-src" ,(package-source intervaltree)) ("multichoose-src" ,(package-source multichoose)))) (arguments `(#:tests? #f ; no tests #:phases (modify-phases %standard-phases (add-after 'unpack 'build-shared-library (lambda _ (substitute* "CMakeLists.txt" (("vcflib STATIC") "vcflib SHARED")) (substitute* "test/Makefile" (("libvcflib.a") "libvcflib.so")) #t)) (add-after 'unpack 'unpack-submodule-sources (lambda* (#:key inputs #:allow-other-keys) (let ((unpack (lambda (source target) (mkdir target) (with-directory-excursion target (if (file-is-directory? (assoc-ref inputs source)) (copy-recursively (assoc-ref inputs source) ".") (invoke "tar" "xvf" (assoc-ref inputs source) "--strip-components=1")))))) (and (unpack "filevercmp-src" "filevercmp") (unpack "fsom-src" "fsom") (unpack "intervaltree-src" "intervaltree") (unpack "multichoose-src" "multichoose")) #t))) ;; This pkg-config file is provided by other distributions. (add-after 'install 'install-pkg-config-file (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (pkgconfig (string-append out "/lib/pkgconfig"))) (mkdir-p pkgconfig) (with-output-to-file (string-append pkgconfig "/vcflib.pc") (lambda _ (format #t "prefix=~a~@ exec_prefix=${prefix}~@ libdir=${exec_prefix}/lib~@ includedir=${prefix}/include~@ ~@ Name: vcflib~@ Version: ~a~@ Requires: smithwaterman, fastahack, tabixpp~@ Description: C++ library for parsing and manipulating VCF files~@ Libs: -L${libdir} -lvcflib~@ Cflags: -I${includedir}~%" out ,version))) #t)))))) (home-page "https://github.com/vcflib/vcflib/") (synopsis "Library for parsing and manipulating VCF files") (description "Vcflib provides methods to manipulate and interpret sequence variation as it can be described by VCF. It is both an API for parsing and operating on records of genomic variation as it can be described by the VCF format, and a collection of command-line utilities for executing complex manipulations on VCF files.") (license license:expat))) (define-public freebayes (package (name "freebayes") (version "1.3.5") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/freebayes/freebayes") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "1l0z88gq57kva677a6xri5g9k2d9h9lk5yk1q2xmq64wqhv7dvc3")) (modules '((guix build utils))) (snippet '(begin (delete-file-recursively "contrib/htslib") #t)))) (build-system meson-build-system) (inputs `(("fastahack" ,fastahack) ("htslib" ,htslib) ("smithwaterman" ,smithwaterman) ("tabixpp" ,tabixpp) ("vcflib" ,vcflib))) (native-inputs `(("bash-tap" ,bash-tap) ("bc" ,bc) ("grep" ,grep) ; Built with perl support. ("parallel" ,parallel) ("perl" ,perl) ("pkg-config" ,pkg-config) ("samtools" ,samtools) ("simde" ,simde) ;; This submodule is needed to run the tests. ("test-simple-bash-src" ,(origin (method git-fetch) (uri (git-reference (url "https://github.com/ingydotnet/test-simple-bash/") (commit "124673ff204b01c8e96b7fc9f9b32ee35d898acc"))) (file-name "test-simple-bash-src-checkout") (sha256 (base32 "043plp6z0x9yf7mdpky1fw7zcpwn1p47px95w9mh16603zqqqpga")))))) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'patch-source (lambda* (#:key inputs #:allow-other-keys) (let ((bash-tap (assoc-ref inputs "bash-tap"))) (substitute* (find-files "test/t") (("BASH_TAP_ROOT=bash-tap") (string-append "BASH_TAP_ROOT=" bash-tap "/bin")) (("bash-tap/bash-tap-bootstrap") (string-append bash-tap "/bin/bash-tap-bootstrap")) (("source.*bash-tap-bootstrap") (string-append "source " bash-tap "/bin/bash-tap-bootstrap"))) (substitute* '("src/BedReader.cpp" "src/BedReader.h") (("../intervaltree/IntervalTree.h") "IntervalTree.h")) (substitute* "meson.build" ;; Our pkg-config file is vcflib.pc (("libvcflib") "vcflib") (("vcflib_inc,") "")) #t))) (add-after 'unpack 'unpack-submodule-sources (lambda* (#:key inputs #:allow-other-keys) (mkdir-p "test/test-simple-bash") (copy-recursively (assoc-ref inputs "test-simple-bash-src") "test/test-simple-bash") #t)) ;; The slow tests take longer than the specified timeout. ,@(if (any (cute string=? <> (%current-system)) '("armhf-linux" "aarch64-linux")) '((replace 'check (lambda* (#:key tests? #:allow-other-keys) (when tests? (invoke "meson" "test" "--timeout-multiplier" "5")) #t))) '())))) (home-page "https://github.com/freebayes/freebayes") (synopsis "Haplotype-based variant detector") (description "FreeBayes is a Bayesian genetic variant detector designed to find small polymorphisms, specifically SNPs (single-nucleotide polymorphisms), indels (insertions and deletions), MNPs (multi-nucleotide polymorphisms), and complex events (composite insertion and substitution events) smaller than the length of a short-read sequencing alignment.") (license license:expat))) (define-public samblaster (package (name "samblaster") (version "0.1.24") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/GregoryFaust/samblaster") (commit (string-append "v." version)))) (file-name (git-file-name name version)) (sha256 (base32 "0iv2ddfw8363vb2x8gr3p8g88whb6mb9m0pf71i2cqsbv6jghap7")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are none #:phases (modify-phases %standard-phases (delete 'configure) ; There is no configure phase. (replace 'install (lambda* (#:key outputs #:allow-other-keys) (install-file "samblaster" (string-append (assoc-ref outputs "out") "/bin")) #t))))) (home-page "https://github.com/GregoryFaust/samblaster") (synopsis "Mark duplicates in paired-end SAM files") (description "Samblaster is a fast and flexible program for marking duplicates in read-id grouped paired-end SAM files. It can also optionally output discordant read pairs and/or split read mappings to separate SAM files, and/or unmapped/clipped reads to a separate FASTQ file. When marking duplicates, samblaster will require approximately 20MB of memory per 1M read pairs.") (license license:expat))) (define-public r-velocyto (let ((commit "d7790346cb99f49ab9c2b23ba70dcf9d2c9fc350") (revision "1")) (package (name "r-velocyto") (version (git-version "0.6" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/velocyto-team/velocyto.R") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "16wqf70j7rd7pay2q513iyz12i8n9vrpg1bisah4lddbcpx5dz1n")))) (build-system r-build-system) (inputs `(("boost" ,boost))) (propagated-inputs `(("r-hdf5r" ,r-hdf5r) ("r-mass" ,r-mass) ("r-mgcv" ,r-mgcv) ("r-pcamethods" ,r-pcamethods) ("r-rcpp" ,r-rcpp) ("r-rcpparmadillo" ,r-rcpparmadillo) ;; Suggested packages ("r-rtsne" ,r-rtsne) ("r-cluster" ,r-cluster) ("r-abind" ,r-abind) ("r-h5" ,r-h5) ("r-biocgenerics" ,r-biocgenerics) ("r-genomicalignments" ,r-genomicalignments) ("r-rsamtools" ,r-rsamtools) ("r-edger" ,r-edger) ("r-igraph" ,r-igraph))) (home-page "https://velocyto.org") (synopsis "RNA velocity estimation in R") (description "This package provides basic routines for estimation of gene-specific transcriptional derivatives and visualization of the resulting velocity patterns.") (license license:gpl3)))) (define-public methyldackel (package (name "methyldackel") (version "0.5.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/dpryan79/MethylDackel") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1sfhf2ap75qxpnmy1ifgmxqs18rq8mah9mcgkby73vc6h0sw99ws")))) (build-system gnu-build-system) (arguments `(#:test-target "test" #:make-flags (list "CC=gcc" (string-append "prefix=" (assoc-ref %outputs "out") "/bin/")) #:phases (modify-phases %standard-phases (replace 'configure (lambda* (#:key outputs #:allow-other-keys) (substitute* "Makefile" (("-lhts ") "-lhts -lBigWig ") (("install MethylDackel \\$\\(prefix\\)" match) (string-append "install -d $(prefix); " match))) #t))))) (inputs `(("curl" ,curl) ; XXX: needed by libbigwig ("htslib" ,htslib-1.9) ("libbigwig" ,libbigwig) ("zlib" ,zlib))) ;; Needed for tests (native-inputs `(("python" ,python-wrapper))) (home-page "https://github.com/dpryan79/MethylDackel") (synopsis "Universal methylation extractor for BS-seq experiments") (description "MethylDackel will process a coordinate-sorted and indexed BAM or CRAM file containing some form of BS-seq alignments and extract per-base methylation metrics from them. MethylDackel requires an indexed fasta file containing the reference genome as well.") ;; See https://github.com/dpryan79/MethylDackel/issues/85 (license license:expat))) ;; This package bundles PCRE 8.02 and cannot be built with the current ;; version. (define-public phast (package (name "phast") (version "1.5") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/CshlSiepelLab/phast") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "10lpbllvny923jjbbyrpxahhd1m5h7sbj9gx7rd123rg10mlidki")))) (build-system gnu-build-system) (arguments `(#:make-flags (list "CC=gcc" (string-append "DESTDIR=" (assoc-ref %outputs "out"))) #:phases (modify-phases %standard-phases (replace 'configure (lambda* (#:key inputs outputs #:allow-other-keys) ;; Fix syntax (substitute* "test/Makefile" ((" ") " ")) (substitute* "Makefile" (("CLAPACKPATH=/usr/lib") (string-append "CLAPACKPATH=" (assoc-ref inputs "clapack") "/lib"))) ;; Renaming the libraries is not necessary with our version of ;; CLAPACK. (substitute* "src/lib/Makefile" (("ifdef CLAPACKPATH") "ifdef UNNECESSARY")) (substitute* "src/make-include.mk" (("-lblaswr") "-lblas") (("-ltmg") "-ltmglib") (("liblapack.a") "liblapack.so") (("libblas.a") "libblas.so") (("libf2c.a") "libf2c.so")) (substitute* "src/Makefile" (("/opt") "/share") (("/usr/") "/")) #t)) (replace 'check (lambda _ (setenv "PATH" (string-append (getcwd) "/bin:" (getenv "PATH"))) ;; Disable broken test (substitute* "test/Makefile" ((".*if.*hmrc_summary" m) (string-append "#" m))) ;; Only run the msa_view tests because the others fail for ;; unknown reasons. (invoke "make" "-C" "test" "msa_view")))))) (inputs `(("clapack" ,clapack))) (native-inputs `(("perl" ,perl))) (home-page "http://compgen.cshl.edu/phast/") (synopsis "Phylogenetic analysis with space/time models") (description "Phylogenetic Analysis with Space/Time models (PHAST) is a collection of command-line programs and supporting libraries for comparative and evolutionary genomics. Best known as the search engine behind the Conservation tracks in the University of California, Santa Cruz (UCSC) Genome Browser, PHAST also includes several tools for phylogenetic modeling, functional element identification, as well as utilities for manipulating alignments, trees and genomic annotations.") (license license:bsd-3))) (define-public python-gffutils ;; The latest release is older more than a year than the latest commit (let ((commit "4034c54600813b1402945e12faa91b3a53162cf1") (revision "1")) (package (name "python-gffutils") (version (git-version "0.9" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/daler/gffutils") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "1rwafjdnbir5wnk0ap06ww4lra3p5frhy7mfs03rlldgfnwxymsn")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (replace 'check (lambda _ ;; Tests need to access the HOME directory (setenv "HOME" "/tmp") (invoke "nosetests" "-a" "!slow"))) (add-after 'unpack 'make-gz-files-writable (lambda _ (for-each make-file-writable (find-files "." "\\.gz")) #t))))) (propagated-inputs `(("python-argcomplete" ,python-argcomplete) ("python-argh" ,python-argh) ("python-biopython" ,python-biopython) ("python-pybedtools" ,python-pybedtools) ("python-pyfaidx" ,python-pyfaidx) ("python-simplejson" ,python-simplejson) ("python-six" ,python-six))) (native-inputs `(("python-nose" , python-nose))) (home-page "https://github.com/daler/gffutils") (synopsis "Tool for manipulation of GFF and GTF files") (description "python-gffutils is a Python package for working with and manipulating the GFF and GTF format files typically used for genomic annotations. The files are loaded into a SQLite database, allowing much more complex manipulation of hierarchical features (e.g., genes, transcripts, and exons) than is possible with plain-text methods alone.") (license license:expat)))) (define-public indelfixer (package (name "indelfixer") (version "1.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/cbg-ethz/InDelFixer/") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "10ak05x8i1bx2p7rriv2rglqg1wr7c8wrhjrqlq1wm7ka99w8i79")))) (build-system ant-build-system) (arguments `(#:jar-name "InDelFixer.jar" #:source-dir "src/main/java" #:test-dir "src/test")) (inputs `(("java-commons-lang2" ,java-commons-lang) ("java-args4j" ,java-args4j))) (native-inputs `(("java-junit" ,java-junit))) (home-page "https://github.com/cbg-ethz/InDelFixer/") (synopsis "Iterative and sensitive NGS sequence aligner") (description "InDelFixer is a sensitive aligner for 454, Illumina and PacBio data, employing a full Smith-Waterman alignment against a reference. This Java command line application aligns Next-Generation Sequencing (NGS) and third-generation reads to a set of reference sequences, by a prior fast k-mer matching and removes indels, causing frame shifts. In addition, only a specific region can be considered. An iterative refinement of the alignment can be performed, by alignment against the consensus sequence with wobbles. The output is in SAM format.") (license license:gpl3+))) (define-public libsbml (package (name "libsbml") (version "5.18.0") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/sbml/libsbml/" version "/stable/libSBML-" version "-core-src.tar.gz")) (sha256 (base32 "0slkagrk3nfi2qsksv6b1brj6zhx4bj4bkib2sdycvrcd10ql2lh")))) (build-system cmake-build-system) (arguments `(#:test-target "test" #:configure-flags (list "-DWITH_CHECK=ON" (string-append "-DLIBXML_LIBRARY=" (assoc-ref %build-inputs "libxml2") "/lib/libxml2.so") (string-append "-DLIBXML_INCLUDE_DIR=" (assoc-ref %build-inputs "libxml2") "/include/libxml2")))) (propagated-inputs `(("libxml2" ,libxml2))) (native-inputs `(("check" ,check-0.14) ("swig" ,swig))) (home-page "http://sbml.org/Software/libSBML") (synopsis "Process SBML files and data streams") (description "LibSBML is a library to help you read, write, manipulate, translate, and validate SBML files and data streams. The @dfn{Systems Biology Markup Language} (SBML) is an interchange format for computer models of biological processes. SBML is useful for models of metabolism, cell signaling, and more. It continues to be evolved and expanded by an international community.") (license license:lgpl2.1+))) (define-public kraken2 (package (name "kraken2") (version "2.1.1") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/DerrickWood/kraken2") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0h7a7vygd7y5isbrnc6srwq6xj1rmyd33pm8mmcgfkmlxlg5vkg3")))) (build-system gnu-build-system) (arguments `(#:tests? #false ; there are none #:make-flags (list "-C" "src" (string-append "KRAKEN2_DIR=" (assoc-ref %outputs "out") "/bin")) #:phases (modify-phases %standard-phases (delete 'configure) (add-before 'install 'install-scripts (lambda* (#:key outputs #:allow-other-keys) (let* ((bin (string-append (assoc-ref outputs "out") "/bin")) (replacements `(("KRAKEN2_DIR" . ,bin) ("VERSION" . ,,version)))) (mkdir-p bin) (with-directory-excursion "scripts" (let ((scripts (find-files "." ".*"))) (substitute* scripts (("#####=([^=]+)=#####" _ key) (or (assoc-ref replacements key) (error (format #false "unknown key: ~a~%" key))))) (substitute* "kraken2" (("compression_program = \"bzip2\"") (string-append "compression_program = \"" (which "bzip2") "\"")) (("compression_program = \"gzip\"") (string-append "compression_program = \"" (which "gzip") "\""))) (substitute* '("download_genomic_library.sh" "download_taxonomy.sh" "16S_gg_installation.sh" "16S_silva_installation.sh" "16S_rdp_installation.sh") (("wget") (which "wget"))) (substitute* '("download_taxonomy.sh" "download_genomic_library.sh" "rsync_from_ncbi.pl") (("rsync -") (string-append (which "rsync") " -"))) (substitute* "mask_low_complexity.sh" (("which") (which "which"))) (substitute* '("mask_low_complexity.sh" "download_genomic_library.sh" "16S_silva_installation.sh") (("sed -e ") (string-append (which "sed") " -e "))) (substitute* '("rsync_from_ncbi.pl" "16S_rdp_installation.sh" "16S_silva_installation.sh" "16S_gg_installation.sh" "download_taxonomy.sh" "download_genomic_library.sh") (("gunzip") (which "gunzip"))) (for-each (lambda (script) (chmod script #o555) (install-file script bin)) scripts))))))))) (inputs `(("gzip" ,gzip) ("perl" ,perl) ("rsync" ,rsync) ("sed" ,sed) ("wget" ,wget) ("which" ,which))) (home-page "https://github.com/DerrickWood/kraken2") (synopsis "Taxonomic sequence classification system") (description "Kraken is a taxonomic sequence classifier that assigns taxonomic labels to DNA sequences. Kraken examines the k-mers within a query sequence and uses the information within those k-mers to query a database. That database maps k-mers to the lowest common ancestor (LCA) of all genomes known to contain a given k-mer.") (license license:expat))) (define-public lofreq (package (name "lofreq") (version "2.1.5") (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/CSB5/lofreq") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "0qssrn3mgjak7df6iqc1rljqd3g3a5syvg0lsv4vds43s3fq23bl")))) (build-system gnu-build-system) (arguments '(#:test-target "bug-tests" #:tests? #false)) ; test data are not included (inputs `(("htslib" ,htslib) ("python" ,python-wrapper) ("zlib" ,zlib))) (native-inputs `(("autoconf" ,autoconf) ("automake" ,automake) ("which" ,which))) (home-page "https://csb5.github.io/lofreq/") (synopsis "Sensitive variant calling from sequencing data ") (description "LoFreq is a fast and sensitive variant-caller for inferring SNVs and indels from next-generation sequencing data. It makes full use of base-call qualities and other sources of errors inherent in sequencing (e.g. mapping or base/indel alignment uncertainty), which are usually ignored by other methods or only used for filtering.") (license license:expat))) (define-public python-pyliftover (package (name "python-pyliftover") (version "0.4") ;; The version of pypi does not include test data. (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/konstantint/pyliftover") (commit version))) (file-name (git-file-name name version)) (sha256 (base32 "1j8jp9iynv2l3jv5pr0pn0p3azlama1bqg233piglzm6bqh3m2m3")))) (build-system python-build-system) (arguments `(#:tests? #false)) ; the tests access the web (native-inputs `(("python-pytest" ,python-pytest))) (home-page "https://github.com/konstantint/pyliftover") (synopsis "Python implementation of UCSC liftOver genome coordinate conversion") (description "PyLiftover is a library for quick and easy conversion of genomic (point) coordinates between different assemblies.") (license license:expat))) (define-public python-cgatcore (package (name "python-cgatcore") (version "0.6.7") ;; The version of pypi does not include test data. (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/cgat-developers/cgat-core") (commit (string-append "v" version)))) (file-name (git-file-name name version)) (sha256 (base32 "17vk88v1bx7x02ibzkc9i7ir4b5p1hcjr38jpsfzyzxr68352d5k")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'fix-references (lambda _ (substitute* "cgatcore/pipeline/execution.py" (("#!/bin/bash") (string-append "#!" (which "bash"))) (("executable=\"/bin/bash\"") (string-append "executable=\"" (which "bash") "\"")) (("\\\\time") (which "time"))))) (delete 'check) (add-after 'install 'check (lambda* (#:key tests? inputs outputs #:allow-other-keys) (when tests? (add-installed-pythonpath inputs outputs) ;; Requires network access (delete-file "tests/test_pipeline_execution.py") (invoke "python" "-m" "pytest" "-v"))))))) (native-inputs `(("python-pytest" ,python-pytest) ("lsof" ,lsof) ("hostname" ,inetutils) ("openssl" ,openssl))) (inputs `(("time" ,time))) (propagated-inputs `(("python-apsw" ,python-apsw) ("python-gevent" ,python-gevent) ("python-pandas" ,python-pandas) ("python-paramiko" ,python-paramiko) ("python-pyyaml" ,python-pyyaml) ("python-ruffus" ,python-ruffus) ("python-sqlalchemy" ,python-sqlalchemy))) (home-page "https://github.com/cgat-developers/cgat-core") (synopsis "Computational genomics analysis toolkit") (description "CGAT-core is a set of libraries and helper functions used to enable researchers to design and build computational workflows for the analysis of large-scale data-analysis.") (license license:expat))) (define-public ensembl-vep (let* ((api-version "103") (api-module (lambda (name hash) (origin (method git-fetch) (uri (git-reference (url (string-append "https://github.com/Ensembl/" name ".git")) (commit (string-append "release/" api-version)))) (file-name (string-append name "-" api-version "-checkout")) (sha256 (base32 hash)))))) (package (name "ensembl-vep") (version (string-append api-version ".1")) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/Ensembl/ensembl-vep.git") (commit (string-append "release/" version)))) (sha256 (base32 "1iq7p72cv9b38jz2v8a4slzy2n8y0md487943180ym9xc8qvw09c")))) (build-system gnu-build-system) (arguments `(#:modules ((guix build gnu-build-system) (guix build utils) (ice-9 match)) #:phases (modify-phases %standard-phases (delete 'configure) (delete 'build) ;; Tests need to run after installation (delete 'check) (replace 'install (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((modules '(("ensembl" "/") ("ensembl-variation" "/Variation") ("ensembl-funcgen" "/Funcgen") ("ensembl-io" "/"))) (scripts '(("convert_cache.pl" "vep_convert_cache.pl") ("INSTALL.pl" "vep_install.pl") ("haplo" #f) ("variant_recoder" #f) ("filter_vep" #f) ("vep" #f))) (out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (perl (string-append out "/lib/perl5/site_perl"))) (for-each (match-lambda ((name path) (let ((dir (string-append perl "/Bio/EnsEMBL" path))) (mkdir-p dir) (copy-recursively (string-append (assoc-ref inputs (string-append "api-module-" name)) "/modules/Bio/EnsEMBL" path) dir)))) modules) (copy-recursively "modules/" perl) (mkdir-p bin) (for-each (match-lambda ((script new-name) (let ((location (string-append bin "/" (or new-name (basename script))))) (copy-file script location) (chmod location #o555) (wrap-program location `("PERL5LIB" ":" prefix (,(getenv "PERL5LIB") ,perl)))))) scripts) ;; Fix path to tools (with-directory-excursion (string-append perl "/Bio/EnsEMBL") (substitute* '("Funcgen/RunnableDB/ProbeMapping/PrePipelineChecks.pm" "VEP/BaseRunner.pm" "VEP/Utils.pm" "VEP/AnnotationSource/Cache/VariationTabix.pm" "VEP/AnnotationSource/Cache/BaseSerialized.pm" "Variation/Utils/BaseVepTabixPlugin.pm" "Variation/Utils/VEP.pm" "Variation/Pipeline/ReleaseDataDumps/PreRunChecks.pm") (("`which") (string-append "`" (assoc-ref inputs "which") "/bin/which"))))))) (add-after 'install 'check (lambda* (#:key tests? inputs outputs #:allow-other-keys) (when tests? (setenv "PERL5LIB" (string-append (getenv "PERL5LIB") ":" (assoc-ref outputs "out") "/lib/perl5/site_perl")) (copy-recursively (string-append (assoc-ref inputs "source") "/t") "/tmp/t") (for-each make-file-writable (find-files "/tmp/t")) ;; TODO: haplo needs Set/IntervalTree.pm (invoke "perl" "-e" (string-append " use Test::Harness; use Test::Exception; my $dirname = \"/tmp\"; opendir TEST, \"$dirname\\/t\"; my @test_files = map {\"$dirname\\/t\\/\".$_} grep {!/^\\./ && /\\.t$/} readdir TEST; closedir TEST; @test_files = grep {!/Haplo/} @test_files; runtests(@test_files); ")))))))) (inputs `(("bioperl-minimal" ,bioperl-minimal) ("perl-bio-db-hts" ,perl-bio-db-hts) ("perl-dbi" ,perl-dbi) ("perl-dbd-mysql" ,perl-dbd-mysql) ("perl-libwww" ,perl-libwww) ("perl-http-tiny" ,perl-http-tiny) ("perl-json" ,perl-json) ("which" ,which))) (propagated-inputs `(("kentutils" ,kentutils))) (native-inputs `(("unzip" ,unzip) ("perl" ,perl) ("api-module-ensembl" ,(api-module "ensembl" "0s59rj905g72hljzfpvnx5nxwz925b917y4jp912i23f5gwxh14v")) ("api-module-ensembl-variation" ,(api-module "ensembl-variation" "1dvwdzzfjhzymq02b6n4p6j3a9q4jgq0g89hs7hj1apd7zhirgkq")) ("api-module-ensembl-funcgen" ,(api-module "ensembl-funcgen" "1x23pv38dmv0w0gby6rv3wds50qghb4v3v1mf43vk55msfxzry8n")) ("api-module-ensembl-io" ,(api-module "ensembl-io" "14adb2x934lzsq20035mazdkhrkcw0qzb0xhz6zps9vk4wixwaix")) ("perl-test-harness" ,perl-test-harness) ("perl-test-exception" ,perl-test-exception))) (home-page "http://www.ensembl.org/vep") (synopsis "Predict functional effects of genomic variants") (description "This package provides a Variant Effect Predictor, which predicts the functional effects of genomic variants. It also provides Haplosaurus, which uses phased genotype data to predict whole-transcript haplotype sequences, and Variant Recoder, which translates between different variant encodings.") (license license:asl2.0)))) (define-public r-signac (let ((commit "e0512d348adeda4a3f23a2e8f56d1fe09840e03c") (revision "1")) (package (name "r-signac") (version (git-version "1.1.1" revision commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/timoast/signac/") (commit commit))) (file-name (git-file-name name version)) (sha256 (base32 "1yihhrv7zs87ax61la1nb4y12lg3knraw4b20k5digbcwm8488lb")))) (properties `((upstream-name . "Signac"))) (build-system r-build-system) (inputs `(("zlib" ,zlib))) (propagated-inputs `(("r-annotationfilter" ,r-annotationfilter) ("r-biocgenerics" ,r-biocgenerics) ("r-biostrings" ,r-biostrings) ("r-biovizbase" ,r-biovizbase) ("r-data-table" ,r-data-table) ("r-dplyr" ,r-dplyr) ("r-fastmatch" ,r-fastmatch) ("r-future" ,r-future) ("r-future-apply" ,r-future-apply) ("r-genomeinfodb" ,r-genomeinfodb) ("r-genomicranges" ,r-genomicranges) ("r-ggbio" ,r-ggbio) ("r-ggforce" ,r-ggforce) ("r-ggplot2" ,r-ggplot2) ("r-ggrepel" ,r-ggrepel) ("r-ggseqlogo" ,r-ggseqlogo) ("r-iranges" ,r-iranges) ("r-irlba" ,r-irlba) ("r-lsa" ,r-lsa) ("r-matrix" ,r-matrix) ("r-patchwork" ,r-patchwork) ("r-pbapply" ,r-pbapply) ("r-rcpp" ,r-rcpp) ("r-rcpproll" ,r-rcpproll) ("r-rsamtools" ,r-rsamtools) ("r-s4vectors" ,r-s4vectors) ("r-scales" ,r-scales) ("r-seurat" ,r-seurat) ("r-seuratobject" ,r-seuratobject) ("r-stringi" ,r-stringi) ("r-tidyr" ,r-tidyr))) (home-page "https://github.com/timoast/signac/") (synopsis "Analysis of single-cell chromatin data") (description "This package provides a framework for the analysis and exploration of single-cell chromatin data. The Signac package contains functions for quantifying single-cell chromatin data, computing per-cell quality control metrics, dimension reduction and normalization, visualization, and DNA sequence motif analysis.") (license license:expat))))